Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 438,792 C→A 8.1% intergenic (‑58/‑48) gabR ← / → gabT transcriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943438,7920CA 8.1% 956.5 / 13.3 398intergenic (‑58/‑48)gabR/gabTtranscriptional regulator GabR/4‑aminobutyrate‑‑2‑oxoglutarate transaminase
Reads supporting (aligned to +/- strand):  ref base C (212/138);  new base A (2/46);  total (214/184)
Fisher's exact test for biased strand distribution p-value = 5.24e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

TTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCA  >  NZ_CP034943/438653‑438930
                                                                                                                                           |                                                                                                                                          
tttCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATAcc                                                                                                                                            <  1:1044506/140‑1 (MQ=255)
 ttCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCa                                                                                                                                           >  1:3169914/1‑140 (MQ=255)
 ttCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCa                                                                                                                                           >  2:4305949/1‑140 (MQ=255)
   cTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCaaa                                                                                                                                         <  1:2316398/140‑1 (MQ=255)
     ttttttAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCaaaaa                                                                                                                                       <  1:5088566/140‑1 (MQ=255)
             tttttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAg                                                                                                                               <  2:7658247/140‑1 (MQ=255)
             tttttGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAg                                                                                                                               <  1:5007085/140‑1 (MQ=255)
                  gATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATgg                                                                                                                          <  2:7196607/140‑1 (MQ=255)
                              gatatataTAATCGGATTGTTCTGAGGGGTCGTGTGTGAATGTGATGTCAATTTGCAGATTCTCCTTCTGTTACAATAAAAAATTTATATTTGTTACTTTTCATCATACAAAAAAGAAGTCACAATGGTAAGAAAATAGc                                                                                                              <  2:2275/140‑1 (MQ=255)
                                    tttaATCGGCTTGTTCTGAGCGGTCGATTGTCATTGTTATGTCCATTTGCAGATTCTCCTTATGTTACCATCAAAAATTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCATAATGGTAATAAAATAGCGACAgg                                                                                                        <  2:3815704/138‑1 (MQ=255)
                                    tataATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATTTCCATTTGCAGATTCTCCTTCTGGTACAAACAAAAAGTTATATTTGGTACTTTTCATCATACCAAAAAGAAGAAAGAATGGTAAGAAAATAGCGACAgg                                                                                                        <  2:5641362/140‑1 (MQ=255)
                                     ataATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggg                                                                                                       >  2:1044294/1‑140 (MQ=255)
                                     ataATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggg                                                                                                       >  2:7106560/1‑140 (MQ=255)
                                     ataATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAggg                                                                                                       >  2:2662768/1‑140 (MQ=255)
                                         tCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGAc                                                                                                   >  1:3959262/1‑140 (MQ=255)
                                          gggCTTGTTCTGAGCGGTCGAGGGTGAATGTGATGTCCAGTTGCAGATTCTCCTGCTGGTACCATAAAAAAGTTATAATTGGTACTTTTAATCATACCAAAAACAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                  <  2:6868961/139‑1 (MQ=255)
                                          cGGGTTGTTCTGAGCGGGCGAGTGTGAATGTGATGTCCATTTGCAGTTTGTCATTCTGGTACAATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACa                                                                                                  <  2:6025531/140‑1 (MQ=255)
                                          cGGATTGTTCTGAGCGGTCGTGTGTTAATGTGTTGTCCTTTTGCTGATTCTCCTTCTGGTACCATCAAAAAGTTATAATGGGTACTTTTCATCATACCAAAAAGAAGTAAGAATGGTAAGAAAATCGCGACAGGGGGACa                                                                                                  <  2:3099931/140‑1 (MQ=255)
                                            gCTTGTTCTGAGCGGTCGCGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATAGCAAAATGAAGTCAGCATGGTAAGAAAATAGCGCCAGGGGGACATa                                                                                                >  2:6320333/1‑140 (MQ=255)
                                             cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTAGCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                               >  1:4791664/1‑140 (MQ=255)
                                             cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                               >  1:5722338/1‑140 (MQ=255)
                                             cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                               >  1:36023/1‑140 (MQ=255)
                                             cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                               >  2:5186387/1‑140 (MQ=255)
                                             cTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAc                                                                                               >  2:6163442/1‑140 (MQ=255)
                                               tGTTCTGCGGGCTGGAGTGTTATTTTGATGTCAATTTGCAGAGACACCTTCTGGTCCCAAAAAAATTTTATATTTGTTACTTTTAAACACACAAAAAACAAGTCAGAATGTAAAGAAAACAGCGACAGGGGGACATACGc                                                                                             <  1:3788993/140‑1 (MQ=255)
                                                gTTCTGAGGGGTCGAGTGTGAATTTTATGTCCTTTTCCAGATTCGCCTTCTGGTACCATAAAAAATTTATAATTGGTACTTTTCATCTCACCAAAACGAACTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCa                                                                                            <  2:2923103/140‑1 (MQ=255)
                                                    tGAGCGGTCGTGTGTGAATGTGATGTCCTTTTGCAGATTCTCCTTCTGTTACCATAAAAATTTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGATTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAg                                                                                        <  2:3505090/140‑1 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTACGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  2:2356236/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  2:3970494/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  2:1865914/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  1:1454627/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  1:5857484/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAAGGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  2:5941591/1‑140 (MQ=255)
                                                      aGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATAACAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTc                                                                                      >  1:2102746/1‑140 (MQ=255)
                                                         tgtgtaGTGTGTATTTGTTGTCCATTGGCAGTTTCTCCTTCGGGTACCATAAAAAAGTTATAATTGTTAATTTTCTTAATACCAAAAATAAGTCAGATTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCaaa                                                                                   <  2:3887441/135‑1 (MQ=255)
                                                         gttCGAGTTTGAATGTGAGGTCCTTTTGATTTTTATCCTTCTGGTACAAAAAAAATTTTATAATTGTTACTTTTAATCTTCCAAAAAAGAAGTCAGAAGGCAAAGAAAATAGCGACAGGGGGACATACGCATGAGTCaaa                                                                                   <  1:1585152/138‑1 (MQ=255)
                                                         gtgggAGTTTTAATGTTTTGTCCTTTCGCAGTTTCTCCTTCTGGAACCATAAAAAAGTTATAATTGCTCCTTTTAAACATACCAAAAAGAAGTAAGATTGGTAATAAAACAGCGACAGGGGGACATACGCATGAGTCaaa                                                                                   <  1:828931/136‑1 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATgg                                                                                                                          >  1:7484386/1‑99 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  2:5269063/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  1:1326717/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  2:6711752/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  2:652029/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  1:735093/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  1:6274982/1‑140 (MQ=255)
                                                           tCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaaca                                                                                 >  1:6019596/1‑140 (MQ=255)
                                                              aGTGTGAATCTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGTTACTTTTCAACATACAAAAAAGAAGTCAGAATGGTAATAAAATAGAGACAGAGGGACAAACGCATGATTCAaacaaaa                                                                              >  2:7154857/1‑138 (MQ=255)
                                                               ttgtgAATGTTATGTCCATTTGCATTTTCTCCTTCTGGTACCATAAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCATACTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACag                                                                             <  2:1176176/139‑1 (MQ=255)
                                                                tgtgAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagc                                                                            >  2:2877594/1‑140 (MQ=255)
                                                                 tttattgtgATGTCAATTTTCAGTTTCTCCTTCTGGAACCAAAAAAAATTTTTATTTGGAAATTTTAATAACAAAAAAAACAAGTCAGATTGTAAACAAAATCGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                           <  1:3005949/135‑1 (MQ=255)
                                                                 tttaaTGTTATTTCCATTTGAATATTATCATTCTGTTACCATAAAAAATTTATAATTGGTAATTTTCATCATACCAAAAATAATTCAGAATGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                           <  2:2255913/137‑1 (MQ=255)
                                                                 gtgAATGTTTTGTCCTTTTGCAGAGTCTCCTTCTGGTACCACAAAAATTTTATACTTGGTACTTTTCACAATACCAAAAAGAAGTCATAATGTTAACAAAAAAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                           <  1:4249750/140‑1 (MQ=255)
                                                                 gtgAATGTTATTTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTAATTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                           <  2:5430447/140‑1 (MQ=255)
                                                                 gtgAATGTTATGTCCATTTGCAGATTCTCCTTCTGGTAACATCAAAAATTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagca                                                                           <  2:4627489/140‑1 (MQ=255)
                                                                     aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                       >  2:154229/1‑140 (MQ=255)
                                                                     aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                       >  2:5783160/1‑140 (MQ=255)
                                                                     aTGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACagcaagca                                                                       >  1:1887969/1‑140 (MQ=255)
                                                                      tgttATGTCCATTTGCATATTCTCCTTCTGGTACAATCAAAATGTTATAATTGGTACTTTTCATCATACCAAAAACAAGTCAGATTGGAAAGAAAATCGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                      <  2:1997906/140‑1 (MQ=255)
                                                                      tgtgATGGCCCGTGGCACATTCTCCTTCTGGAACCAACAAAAAGTCAAAATGGGTCCTTTTCATCATACAAAAACCATCGCAGAAGGGTACGAAAATCGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCAt                                                                      <  2:469249/140‑1 (MQ=255)
                                                                         gATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCAc                                                                   >  1:4113390/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTAGTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCCTGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:6169053/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:3438579/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:4326445/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:4947634/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:6388539/1‑140 (MQ=255)
                                                                          aTGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTATCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAAAAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACg                                                                  >  1:7420486/1‑140 (MQ=255)
                                                                           tGTCAATTTGCAGTTTCTCCTTCTGTCACAATAAAAATTTTATAATTGTTACTTTTCACCATACCAAAAATAAGTCAGATTGGTAACAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGa                                                                 <  1:4507143/140‑1 (MQ=255)
                                                                              ccATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTTGTACTTTTCATCATACAAAAAAGAAGTCAGAATGGTAAGAAAATAGAGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTg                                                              >  2:3688194/1‑140 (MQ=255)
                                                                               cATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGc                                                             >  2:3309333/1‑140 (MQ=255)
                                                                                 tttGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGccc                                                           >  1:2487500/1‑140 (MQ=255)
                                                                                   tGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAg                                                         >  1:7090488/1‑140 (MQ=255)
                                                                                      ttatgctcCTTGTGCTACCATAAACAAGTTTTAAGTTTTAGTTTACAACATACAAAAAATATCACACATTGGTACGATAATCGCGACAGGGGGACATCCGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTgg                                                      <  1:7045095/135‑1 (MQ=255)
                                                                                       gATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgc                                                     >  1:7194877/1‑140 (MQ=255)
                                                                                          tctcCTTCTGGTACCAAAAAAAATTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGTTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgcagc                                                  <  2:6760524/140‑1 (MQ=255)
                                                                                           ctcCTTCTGGTACCATCAAAAATTTATAATTGGTACTTTTCAACATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGgcagca                                                 <  2:7223588/140‑1 (MQ=255)
                                                                                           cgccTTCTGGTACAAAAAAAATTTTATATGTGTAACTTTTAATCTAACAAATAATAAGACATATTGTTAAGAATAAAGCGACAGGGGGAAATACGCATGAGTCAAACAACAGAAATAATCACGAAGGCCCAGTGgcagca                                                 <  1:1806456/138‑1 (MQ=255)
                                                                                                tCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAAc                                            >  2:2791883/1‑140 (MQ=255)
                                                                                                      aCCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGc                                      >  2:1426302/1‑140 (MQ=255)
                                                                                                        cATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCaa                                    >  1:5620190/1‑140 (MQ=255)
                                                                                                          tCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAAtt                                  >  2:7368657/1‑140 (MQ=255)
                                                                                                           cAAAAAGTTATATTTGGTCCTTGTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                 <  2:4514978/140‑1 (MQ=255)
                                                                                                           cAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                 <  2:7535537/140‑1 (MQ=255)
                                                                                                           aaaaaaGTTATAATTGGTAATTTTCAACATACCAAAAAGAAGTCAGAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                 <  2:7069495/139‑1 (MQ=255)
                                                                                                                  ttATAATTGTTAATTTTCATCATACCAAAAAGAAGTCACAATGGTAAGAAAAGAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACGGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCa                          <  2:2155017/140‑1 (MQ=255)
                                                                                                                    ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAaac                                                                                  <  2:1775820/82‑1 (MQ=255)
                                                                                                                    ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                 <  2:1642402/131‑1 (MQ=255)
                                                                                                                    ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTc                                 >  1:1642987/1‑131 (MQ=255)
                                                                                                                    ataATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCaaa                        >  1:3481502/1‑140 (MQ=255)
                                                                                                                       aTTGGTACTTTTCATCATACCAAAAAGAATTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGgt                     <  2:2553486/140‑1 (MQ=255)
                                                                                                                       aTTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGgt                     <  2:4379660/140‑1 (MQ=255)
                                                                                                                       aTTGGTACTTTTAATCATACAAAAAACAAGTCAGAAGGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGgt                     <  2:6215762/140‑1 (MQ=255)
                                                                                                                          ggTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGtgtg                  >  1:1226640/1‑140 (MQ=255)
                                                                                                                          ggTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATAAGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTAGTCTCGAAAGGtgtg                  >  2:138401/1‑140 (MQ=255)
                                                                                                                            tACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGCGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAg                >  1:544723/1‑140 (MQ=255)
                                                                                                                              cTTTTCATAATACAAAAAAGAAGTCAGACTGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCa              <  2:5044697/140‑1 (MQ=255)
                                                                                                                                  tcatcaTACCAAAATTAATTCAGAATGTTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACgg          <  2:4209068/140‑1 (MQ=255)
                                                                                                                                   catcagACCAAAAAGAAGTCAGAATGGTAAGAAAATCGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGc         <  2:2069116/140‑1 (MQ=255)
                                                                                                                                        tACAAAAAATAAGACATAATGGTAATAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCg    <  1:6646943/140‑1 (MQ=255)
                                                                                                                                        gaCCAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCg    <  1:3389643/139‑1 (MQ=255)
                                                                                                                                          ccAAAAATAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCa  <  2:4955222/140‑1 (MQ=255)
                                                                                                                                          ccAAAAAGAAGTCCGACTGGTACGAAAATAGCGACAGGGGGACATACGCATGAGTCAACCAACCGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCa  <  2:3219098/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TTTCTTTTTTTAGTTTTTGATAAATTTGCTGATAGATATAATCGGCTTGTTCTGAGCGGTCGAGTGTGAATGTGATGTCCATTTGCAGATTCTCCTTCTGGTACCATCAAAAAGTTATAATTGGTACTTTTCATCATACCAAAAAGAAGTCAGAATGGTAAGAAAATAGCGACAGGGGGACATACGCATGAGTCAAACAACAGCAAGCATCACGACTGCCCAGTGGCAGCAAAAACGGGAGCAATTCGTCTCCAAAGGTGTGAGCAACGGCAACCGCA  >  NZ_CP034943/438653‑438930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: