Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,119 T→G 14.4% L44R (CTG→CGG)  EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1190TG14.4% 1024.1 / 35.2 469L44R (CTG→CGG) EO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (211/167);  new base G (4/85);  total (216/253)
Fisher's exact test for biased strand distribution p-value = 2.40e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

ACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATA  >  NZ_CP034943/548980‑549258
                                                                                                                                           |                                                                                                                                           
acaacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCt                                                                                                                                             <  2:1468311/140‑1 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                            >  1:1832002/1‑140 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                            >  2:2625563/1‑140 (MQ=255)
 caacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTg                                                                                                                                            >  2:4265193/1‑140 (MQ=255)
  aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGGTTTATCGCTATTTCAAAGAAAACCTGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                           >  2:4844220/1‑140 (MQ=255)
  aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGTAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                           >  2:1954648/1‑140 (MQ=255)
  aacCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                           >  1:1954494/1‑140 (MQ=255)
  aacCCAAATGGAGATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTgg                                                                                                                                           >  2:4593338/1‑140 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                         <  1:3488614/140‑1 (MQ=255)
    cccAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCg                                                                                                                                         >  1:2715623/1‑140 (MQ=255)
         aTGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCa                                                                                                                                    >  1:322896/1‑140 (MQ=255)
            gATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCGCCCTTCCAAAAGACTCCTGGCGAGTCAGCt                                                                                                                                 <  2:6127623/140‑1 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGACTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                               >  2:1352951/1‑140 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTc                                                                                                                                     <  1:3380412/134‑1 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                               >  1:2323505/1‑140 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                               >  1:1555422/1‑140 (MQ=255)
              tatCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTGTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCttt                                                                                                                               >  1:6226185/1‑140 (MQ=255)
                 cccgacTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                            <  1:4725308/135‑1 (MQ=255)
                 cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                            <  1:3849562/140‑1 (MQ=255)
                 cACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                            >  2:5411147/1‑140 (MQ=255)
                 cACGCCGTTTTTGACTAGAACTCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCa                                                                                                                            <  2:1884771/140‑1 (MQ=255)
                   cGCCTTTTTTGAATATAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGCGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                          <  1:7031383/140‑1 (MQ=255)
                   cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGCCTCCTGGCGAGTCAGCTTTCCATc                                                                                                                          <  2:3817629/140‑1 (MQ=255)
                   cGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                          <  2:2339193/140‑1 (MQ=255)
                        tttttGAATATAATTCTTGATAGACCGCTTTATCAGCAGCTTTATCGATTTTTCAAACAAAACATGCACCCGGGGCGTATTAAAAAAGCAATGAAGCTCCCTACAAAAATACTCCTGGCGAGTCAGCTTTCCATCATTCa                                                                                                                     <  2:6637219/140‑1 (MQ=255)
                         ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                                                                                    >  1:4346715/1‑140 (MQ=255)
                         ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                                                                                    >  2:673927/1‑140 (MQ=255)
                         ttttGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCaa                                                                                                                    >  1:6069984/1‑140 (MQ=255)
                          ttttAATAGAAATCTGGATTTTCCGCTTTATCCGCAGCTTTATCGCAATTTCAACGAAAACAGGCATCCGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCAACAGTCaca                                                                                                                   <  2:5696943/140‑3 (MQ=255)
                           tttaaTAGAATTGGGGATATTCCGCTTTATCAGCATCTTTCTCGATATTTAAAATAAAACTTGCATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGCTTTCCATCCGTCAAAc                                                                                                                  <  2:7064069/137‑1 (MQ=255)
                           ttGAATAGAAAGCGTGATATTCCGCTTTACAAGCAGCTTTATCGATTTTTCAAACAAAACATCCATCCGGGGCTTATTCAAAAAGCAATGAAGCTCCCTTCCAAAAGACGCCGGGCGAGTCAGCTTCCCATCAGTCacac                                                                                                                  <  2:4232973/140‑1 (MQ=255)
                           ttGAATACAAATCTGTATATTCCGATTTATCAGCAGCTTTATCGCTATTTAAAAGAAAAAATGCATCCGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                                  <  2:6017450/140‑1 (MQ=255)
                                tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAACACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                                                             >  2:2458068/1‑140 (MQ=255)
                                tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGCATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCGTTCCATCAGTCACACGACTg                                                                                                             >  1:1213768/1‑140 (MQ=255)
                                tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTGCCATCAGTCAAACGACTg                                                                                                             >  2:106182/1‑140 (MQ=255)
                                       cTGTATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAACAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCACCAGTCACACGACTGTAGAACg                                                                                                      <  2:5899801/140‑1 (MQ=255)
                                       cTGTATATTCCGATTTATCAGCAGCTTTATCGCTATTAAAAACAAAACATGCATCCGGGGCTTATAAAAAAAGGAAGGAAGCTCCCTTCCAAACCACTCCTGGCGAGTCAGTTTCCCATCAGTAAAACGACTGTAGAACg                                                                                                      <  1:442483/140‑1 (MQ=255)
                                         gtatatTCCGATTTATCAGCAGCTTTATCGATATTTCAAACAAAACATCCATCAGGGGCGTATTCAAAAAGCAATGAAGCTCCCTCCCAAAACACTCCGGGCCAGTCCGCTTCCCATCAGTCAAACGACTGTAGAACGTg                                                                                                    <  1:6590261/138‑1 (MQ=255)
                                            tatTCCGCTTTATCAGCAGCTTTATCTATATTAAAAACAAAAAATCCATCAGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACACCTGGCTAGTCACCTTTCCATCAGTCAAACGACTGTAGAACGTGCCt                                                                                                 <  2:3973356/140‑1 (MQ=255)
                                                 cgatttATCAGCAGCTTTATCGATATTACAAACAAAACATGCATCAGGGGCTTATAAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACGTGCCTATGaa                                                                                            <  2:3610405/137‑1 (MQ=255)
                                                 cGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGaa                                                                                            >  1:7372271/1‑140 (MQ=255)
                                                 cGCTTTATCAGCAGCTTTATCGCTATTACAAAGAAAACATGCAACCGGGGCTTATTCAAAAAGTAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGaa                                                                                            <  2:7370176/140‑1 (MQ=255)
                                                  gCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAAc                                                                                           >  1:817776/1‑140 (MQ=255)
                                                      tacccgcagcTTTATCTATATTTAAAAGAAACCATGCATCTGGGGGGTATACAAAAAGTAATCACGCCCCGTTCCAAAACACTCCGGGCGCGGCTGATTTCCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCt                                                                                       <  1:6394199/135‑1 (MQ=255)
                                                      tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                                                                       >  2:3320748/1‑140 (MQ=255)
                                                        actcaagcTTTATCCCTATTTAAAAGAAACCTTTCATAAGGCGCTTTCACAAAATGGAAAGATGCTCCCTTCCAAATCAATCCTGCCGAGTCAGTTTTCCTTCAGAACCACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                     <  2:5480326/135‑1 (MQ=255)
                                                           gcagcTTTATCGCTCTTTCAAAGAAAACATGCATCAGGGGCGGATGCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCGTTCCATCAGTCAAACGACTGTAGCACGTGCCTCGGAGCAGCTtgctg                                                                                  >  2:4534994/1‑140 (MQ=255)
                                                            cagcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTtgctgc                                                                                 >  2:517877/1‑140 (MQ=255)
                                                             aggtttATCGCAATTTAAAATAAAAAATGCTTCAGGGGCTTTTAAAAAATGGAATGAAGCTCCCTTCAAAACGACACGTGGCTATTAATCTTTCAATCTTTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  2:3110249/137‑1 (MQ=255)
                                                             agcTTTCTCGCTATTAAAAACAAAAAATCCACCCGGGGCGTATTAAAAAAGCAATGACGCGCCCTACCAAAACACCCCTGGCGAGACAGCTTACCACCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  1:3677283/140‑1 (MQ=255)
                                                             agcTTTATCGCTTTTTAAAACAAAACAGGCATCCGGGGCGTAATAAAAAAGTAATCAAGCGCCCTCCCAAACCACTCCTGGCCAGTCAGCTTCCCAACACTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  1:2625563/140‑1 (MQ=255)
                                                             agcTTTATCGCTATTTCAAAGAAAACCTGCACCAGGGGCTTATTCAAAAAGGAATGAAGCGCCCTTCCAAAAGACTCCGGGCGAGACAGCTTTCCACACGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  2:747953/140‑1 (MQ=255)
                                                             agcTTTATCGCTATTACAACCAAAACATCCATCCGGGGCTTATCAAAAAAGGAATGACGCTCCCTTCCAAACCACTCCGGGCGAGACCGGTTCCAAACCGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  1:3598056/140‑1 (MQ=255)
                                                             agcTTTATCGCTATTACAAAGAAAACCTGCATCCGGGGCTTTTACAAAAAGCAATGAAGCTCCCTTCCAAAAGACCCCGGGCGAGTCAGCTTCCCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  2:1114230/140‑1 (MQ=255)
                                                             agcTTTATCGATATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGTAATGACGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCAACCGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  2:2758625/140‑1 (MQ=255)
                                                             agcTTTAGCGATATTTCAACCAAAAAATCCATCAGGGGCTTATACAAAAAGCAATGAAGCGCCATTCCACACCACGCCTGGCGAGTCACGTTACCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                <  1:873434/140‑1 (MQ=255)
                                                              gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                               >  1:4240041/1‑140 (MQ=255)
                                                               cTTTATCGCTTTTTCAAAGAAAACATGCATCCGGGGGGTATTCAAACAGGAATGACGCTCCCTTCCAACAGACTCCGGGGGAGTCAGGTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                              <  2:1240420/140‑1 (MQ=255)
                                                               cTTTATCGCTATTTCAACTAAAACATGCATCAGGGGCGTATTCAAAAAGGATTGACGCTCCCTTCCAAAAGACGCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                              <  2:1987482/140‑1 (MQ=255)
                                                               cTTTATCGCTATTTAAAAGAAAACATGCATCCGGGGGGTATTCAAAAAGGAATGAAGCTCCCTACCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                              <  2:1555422/140‑1 (MQ=255)
                                                                    tctctttttCAAATAAAAACTGCATCTGGGGCTTTTTAAAAATGCAATGACGCGCCCTGCCAAACCACTCCTGGCGATTCTGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                         <  2:6910341/134‑1 (MQ=255)
                                                                    tCGATATTAAAAACAAAAAATGCATCCGGGGCTTATTAAAAAAGTAATGAAGCTCCCTTCCAAAACCCTCCGGGCTCGTCAGCTTGCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                         <  1:673927/140‑1 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATc                                                                                                                          >  2:343823/1‑86 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                    >  1:7291374/1‑140 (MQ=255)
                                                                         aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                    >  2:879207/1‑140 (MQ=255)
                                                                               actcaaaCAGCCATCCGGGGGGTATCAAACAAGTAAGGAAGCCCCCTTCCAAAATACTCCTGGCTATAACGCTTTCCTTCATTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                              <  1:2847680/136‑1 (MQ=255)
                                                                               aataaaaCATGCATCCGGGGGGTATTCAAAAAGGAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCAATCTGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                              <  2:465513/137‑1 (MQ=255)
                                                                               aaGAAAACATGCATCAGGGGCTTATTCAAAAAGTAATGAAGCGCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                              <  2:4098620/140‑1 (MQ=255)
                                                                               aaGAAAACAGGCATCCGGGGCTTATTCAAAAAGGAATGAAGCTCCCTGCCAAACGACTCGTGGGGAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATCTCGTGAGc                                                              <  2:7683766/140‑1 (MQ=255)
                                                                                aGAAAACATGCAGCCGGGGGTTATTCAAAAATTAATGAAGCCCCCTTCCAAAAGACTCGGGGCGAGTAAGCTTTCCATCAGAAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                             <  1:103870/140‑1 (MQ=255)
                                                                                    aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                                                         >  1:1430840/1‑140 (MQ=255)
                                                                                                gggCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATg                                             >  1:6682922/1‑140 (MQ=255)
                                                                                                     tATTCAAAACGCAATGAAGCTCCCTTCAAAAAGCCTCCTGGCGAGTCAGCTTTCCACCATTAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                        <  1:520752/140‑1 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                        >  2:6411687/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                        >  1:6083557/1‑140 (MQ=255)
                                                                                                     tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTAGATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                        >  1:4042748/1‑140 (MQ=255)
                                                                                                       ttCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCt                                      >  1:7544466/1‑140 (MQ=255)
                                                                                                          aaaacGGAATGAAGCTCCCTTCCAAACGACTCCGGGCGAGTCTGCTTTCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                                   <  1:4906776/140‑1 (MQ=255)
                                                                                                            aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                 >  2:1700763/1‑140 (MQ=255)
                                                                                                              aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                               <  2:6548352/140‑1 (MQ=255)
                                                                                                              aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                               <  1:5995345/140‑1 (MQ=255)
                                                                                                                gAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGt                                                                                                                       >  2:2234849/1‑50 (MQ=255)
                                                                                                                 aaTGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATg                            >  2:4316189/1‑140 (MQ=255)
                                                                                                                   tGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGAt                          >  1:2436804/1‑140 (MQ=255)
                                                                                                                     acgcccccTTCCAAACGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTc                        <  1:4674681/135‑1 (MQ=255)
                                                                                                                          cccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                                  <  2:2999923/45‑1 (MQ=255)
                                                                                                                          cccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAc                                                                                                                  >  1:2999923/1‑45 (MQ=255)
                                                                                                                          cccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTCTATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCctgattctgatt                   >  2:5814010/1‑140 (MQ=255)
                                                                                                                           ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                  >  1:5579163/1‑140 (MQ=255)
                                                                                                                               ccAAACGACTCCGGGCCAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg              <  2:6271544/140‑1 (MQ=255)
                                                                                                                               ccAAAAGCCGCCTGGCGAGTAAGCTTTCCAAACGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg              <  2:7267681/140‑1 (MQ=255)
                                                                                                                                 aaaaGACTCGTGGCTTGTCAGCTTTCCATCTGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTa            <  1:456917/140‑1 (MQ=255)
                                                                                                                                      ccTCCGGGCGAGTCAGGTTTCCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTa       <  1:2238958/139‑1 (MQ=255)
                                                                                                                                        tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaa     >  1:5410660/1‑140 (MQ=255)
                                                                                                                                        tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTCCCTATGAACAGCTTGCTGCTGTGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGAGTCTGATTTTGCGTATCGGaaa     >  2:2813525/1‑140 (MQ=255)
                                                                                                                                         ccTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGACTAGGAACAGCTGGCTGATGAGGGCTATATCGTGAGCAAGCCAAGAATCTGATGGTTTGATGATTATCATGATTCTGATATTGCGTATCGTaaaa    >  1:2223635/1‑140 (MQ=255)
                                                                                                                                          cTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAAt   >  1:6846348/1‑140 (MQ=255)
                                                                                                                                           tGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATa  >  1:6478553/1‑140 (MQ=255)
                                                                                                                                           tGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATa  >  1:2096017/1‑140 (MQ=255)
                                                                                                                                           tGGCGAGTCAGCTTTCCATCAGTAAAACGACTGTAGAACGTGCCTATGAACAGATTGCTGCTGAGGGCTATATAGTCAGCAAGCCAAGAATCGGATTGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATa  >  1:575920/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACAACCCAAATGGATATCACGCCTTTTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATA  >  NZ_CP034943/548980‑549258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: