Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 549,144 A→C 16.3% Q52H (CAA→CAC EO946_RS02865 → PLP‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943549,1440AC16.3% 796.3 / 41.7 399Q52H (CAA→CACEO946_RS02865PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (177/140);  new base C (2/80);  total (179/220)
Fisher's exact test for biased strand distribution p-value = 1.51e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 412 total aligned reads displayed.

TTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCAA  >  NZ_CP034943/549005‑549279
                                                                                                                                           |                                                                                                                                       
 ttttAATATAATTCTGTAATTTCCGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACATGCATCCGGGGCGTATAAAAAAAGTAATGAAGCTCCCTTCAAAAACACGCCTGGCGAGTCACCTTTCCATCAGTCaaa                                                                                                                                        <  2:6322255/140‑1 (MQ=255)
 ttttAATAGAAATCTGTATATTCGGCTTTATCAGCAGCTTTATCGCTATTTCAAATAAAACATGCATCAGGGGGGTATTCAAAAAGGAATGAAGCCCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGACaaa                                                                                                                                        <  2:425531/140‑1 (MQ=255)
 tttGAATAGAAATCTGTATATTCCGTTTTATCAGCAGCTTTATCGATTTTTCAAAGAAAACATGCATCAGGGGGGTATTAAAAATGGAATGAAGCTCCCTACCAAAACACTCCGGGCGAGTCAGCTTTCCATCCGACaaa                                                                                                                                        <  2:4346715/140‑1 (MQ=255)
  tttaaTATAAATGTGTATATTCCGCTTTATCAGCAGCTTTATCGATATTTCAAATAAAACATGCATCAGGGGCGTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCCGTCacac                                                                                                                                       <  2:2583614/137‑1 (MQ=255)
       tAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTg                                                                                                                                  >  2:1578025/1‑140 (MQ=255)
              gtgtatatTCCGATTTATCAGCAGCTTTATCGATATTTCAAACAAAACATGCATCCGGGGCTTATTCAAAAAGCAATGAAGCTCCCTTCCAAAACACCCCGGGCGACTCAGCTTACCATCAGACACACGACTGTAGAACg                                                                                                                           <  1:1516346/136‑1 (MQ=255)
              gtGGATTTTCCGCTTTATCAGCAGCTTTATCGCTTTTTCAAATAAAACATGCATCCGGGGCGTATTAAAAAAGGAATGAAGCGCCGTTCCAAACGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                           <  2:407589/139‑1 (MQ=255)
              cgggATATTCCTCTTTATCAGAAGCTTTATCGATATTAAAAATAAAACATGAATCCGGGGCTTATACAAAAAGTAATGAAGCTCCCTTCAAAAACACTCCGGGCGAGTAAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                           <  1:242746/138‑1 (MQ=255)
              cggcatatTCCGGTTTATCAGCAGCTTTATCGTTTTTTCAAATAAAACATGCATCCGGGGGGTTTTCAAAAAGCAATGAAGCTCCATTCAAAAACACTCCTGGCGAGTCCGCTTCCCATCAGGCACACGACTGTAGAACg                                                                                                                           <  1:4113469/136‑1 (MQ=255)
              cTGTATATTCCGATTTATCAGCAGATTTATCGATATTACAAAAAAAACATGCATCCGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACg                                                                                                                           <  1:439929/140‑1 (MQ=255)
              cTGTATATCCCGCTTTATCAGCAGCTTTATCGATATTTCAAACAAAACATGAATCAGGGGCTTATTCAAAAAGTAATGAAGCTCCCTTCCAAAACACTCGTGGCGAGTCAGCTTTCCAACCGTCAAACGACTGTAGAACg                                                                                                                           <  2:6121186/140‑1 (MQ=255)
                   tatTCCTCTTTATCAGAATCTTTTTCTCTATTTAAAATAAAAAATGCATCTGGGGCTTATACAAAAAGTAATGAAGCTCCCTTCAAAAAGACTCCTGGGTAGTCAGTTTTCCATAAGTCAAACGACTGTAGAACGTGCCt                                                                                                                      <  2:1026144/140‑1 (MQ=255)
                    atTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTa                                                                                                                     >  1:2262652/1‑140 (MQ=255)
                      tCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAATAAGGAATGAAGCTCCCTTCCAAAAGAGTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATg                                                                                                                   >  1:6051325/1‑140 (MQ=255)
                        cgatttATCAGCAGCTTTATCGATATTACAAACAAAACATGCATCAGGGGCTTATAAAAAAAGGAATGAAGCTCCCTTCCAAAACACTCCTGGCGAGTCAGCTTTCCATCAGACAAACGACTGTAGAACGTGCCTATGaa                                                                                                                 <  2:3610405/137‑1 (MQ=255)
                        cGCTTAATCACCAGTTTTATCGCTTTTTAAAACAAAACTTGAATCAGGGCCGTTTACAAAAAGGAAGGAAGCTCCCTTCCAAAACACCCCGGGCGAGACTGATTACAAACAGACCCACGACTGTAGAACGTGCCTATGac                                                                                                                 <  1:3644757/140‑2 (MQ=255)
                           tttATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAg                                                                                                              >  1:5655595/1‑140 (MQ=255)
                             tacccgcagcTCTATCCCTATTACAAACACAACATCCAACCGGGGCGTATACAAAACGCAAGGAACCGCCCTTCAAAACGACTCCTGGCGAGTAAGCTTGCCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                                                                                            <  2:512577/135‑1 (MQ=255)
                             tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCt                                                                                                            >  2:3320748/1‑140 (MQ=255)
                             tATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAATAAAAAGCCTTTAGAACGTTCCTTTGAAAAGCt                                                                                                            >  1:844652/1‑140 (MQ=255)
                               tcatcaGCTTTATCGATATTTAAAATAAAACAGCCATCCGGGGCGTATAAAAAAAGGACTGAAGCTCCCTTCCAAACCACCCCGGGCGAGTCAGTTTACCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCTtg                                                                                                          <  1:4264569/140‑1 (MQ=255)
                                  gcagcTTTATCGCTCTTTCAAAGAAAACATGCATCAGGGGCGGATGCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCGTTCCATCAGTCAAACGACTGTAGCACGTGCCTCGGAGCAGCTtgctg                                                                                                       >  2:4534994/1‑140 (MQ=255)
                                   cagcTTTAGCGATATTACAAACAAAACATGCAACAGGGGCGTATTAAAAAAGAAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTAAGCTTTCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTtgctgc                                                                                                      <  2:186755/140‑1 (MQ=255)
                                    tgatttATCGCTATTCAAAATAAAACATGCAGCCGGGGCGTATTAAAAAAGTAATGACGCGCCGTTCAAAAACACGCGGGGCGTGTCAGCTTTCCCTCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:2020513/137‑1 (MQ=255)
                                    atcTTTATCGCTATTTCAAATAAAACAGGCTTCTGGGGCTTATTCAAAAAGTAATTAAGCTCCCTTCCAAAAGCCTCCTGGCGAGTCAGCTTTCCAACAGTAAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:1965920/138‑1 (MQ=255)
                                    agcTTTCTCCATATTTCAAAGAAAACATGCAGCCGGGGCGTATGCAACAAGCAAGGAAGCTCCCTACCAAAACACCCCTGGCGAGACAGCTTTCCAACAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:110646/140‑1 (MQ=255)
                                    agcTTTATCGCTATTTCAAAGAAAACCTGCACCAGGGGCTTATTCAAAAAGGAATGAAGCGCCCTTCCAAAAGACTCCGGGCGAGACAGCTTTCCACACGTCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:747953/140‑1 (MQ=255)
                                    agcTTTATCGCTATTTCAAACAAAACATGCATCAGGGGCTTATTAAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCCGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:1468248/140‑1 (MQ=255)
                                    agcTTTATCGCTATTACAACCAAAACATCCATCCGGGGCTTATCAAAAAAGGAATGACGCTCCCTTCCAAACCACTCCGGGCGAGACCGGTTCCAAACCGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:3598056/140‑1 (MQ=255)
                                    agcTTTATCGATTTTTCAAAGAAAACATGCATCCGGGGCTTATTAAAAACGTAATGACGCTCCATCCCAAAACACTCCTGGCGCGTCAGCTTTCCATCCGACAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:3767055/140‑1 (MQ=255)
                                    agcTTTATCGATATTACAAACAAAACATGCATCCGGGGCGTATTCAAAAAGCAACGAAGCTCCCTTCCAAAACACTCCGGGCGAGTCAGCTTTCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:6227565/140‑1 (MQ=255)
                                    agcTTTAGCGCTTTTACAAACAAACCATGCATCAGGGCCGTATTCAAAAAGGAATGAAGCTCCCTTCCACAAGACGCCGGGGGAGTCAGCTTGCCATCCGCCACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:1803246/140‑1 (MQ=255)
                                    agcTTTAGCGATATTTCAAACAAAACATCCATCCGGGGCTTATAAAAAAAGGATTGAACCCCCCTACCAAACGCCGCGGGGCGAGTCAGCTTCCCACCCGACACACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  1:2551998/140‑1 (MQ=255)
                                    agcTTTACCGCTATTTCAATCACACCCTGCAGCCGGGGCGAATCCAAAACGCAATGAAGCTCCCTTCCAACAGACTCCTGGCCAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:5831601/140‑1 (MQ=255)
                                    agcTTAATCGATATTACAAATAAAAAATGCACCAGGGGCGTATTCAAAAAGGAATGACGCGCCCTTCAAAAAGACTCCGGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTgctgct                                                                                                     <  2:761548/140‑1 (MQ=255)
                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGTCGAGTCAGCTTTCCATAAGTCAAACGACTGTATAACGTGCATATGAACAGCTTGctgctg                                                                                                    >  1:5298146/1‑140 (MQ=255)
                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                                                    >  1:5435433/1‑140 (MQ=255)
                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                                                    >  1:2673890/1‑140 (MQ=255)
                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                                                    >  1:2737100/1‑140 (MQ=255)
                                     gcTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGctgctg                                                                                                    >  1:6631459/1‑140 (MQ=255)
                                      ttttATCGATATTTCAAAGAAAACCTGAATCTGGGGCTTATTCAAAATGGAATCACGCTCCCTTCCAAAAGACTCCGGGCGATTCAGCTTTCCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  1:1321369/139‑1 (MQ=255)
                                      ttttATCGATATTAAAAACAAAACCTCCATCGGGGGCGTATTCAAAACGGACTCACGCTCCCTACAAAACCACTGGGGGCGATTCAGCTCACCAACAGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  1:1186541/139‑1 (MQ=255)
                                      cattAGCGCTCATTCAACCAAAACATCCATCCGGGGCGTCCTCAAAAACCAAAGAAGCTCCATTCCAACAGACCCCTGCCGAGGCAGCTTGCCATCACAAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  2:4005487/138‑1 (MQ=255)
                                      cTTTATCTCAATATCAAAGAAAACAGCAATCCGGGGGTTATAAAAAAACGAATGAAGCTCCCTTCCAAACGACCCCTGGCGAGTCAGCTTTCCATCAGCCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  2:6427796/140‑1 (MQ=255)
                                      cTTTATCGCTTTTTCAAAGAAAACATGCATCCGGGGGGTATTCAAACAGGAATGACGCTCCCTTCCAACAGACTCCGGGGGAGTCAGGTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  2:1240420/140‑1 (MQ=255)
                                      cTTTATCGCTATTTAAAAGAAAACTGGAATCCGGGGCTTTTTAAAAAAGGAATGACGCTCCCTCCAAAAACACGCCTGGCGAGCCAGCTTTCCCCCCGACACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  1:7514633/140‑1 (MQ=255)
                                      cTTTATCGCTATTTAAAAGAAAACATGCATCCGGGGCTTATTAAAAAAGGAATGACGCTCCCTTCCAAACCACGCCGGGCGAGTCAGCTTTCCATCCGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  2:872756/140‑1 (MQ=255)
                                      cTTTATCGCTATTAAAAACAAAACATCCATCCGGGGCTTTTAAAAAAAGGATTGAAGCGCCCTTCCAAAAGACCCCGGGCGAGTCAGCTTGCCACCAGGCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGa                                                                                                   <  1:3054535/140‑1 (MQ=255)
                                          aTCGCATTTTAAAACAAAACATGCACCCGGGGCTTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGACACACGACTGTAGAACGTGCCTATGACCAGCTTGCTGCTGAGGGc                                                                                               <  2:3159073/140‑1 (MQ=255)
                                           tCGCTATTTAAAACAAAACATGCATCGGGGGCTTATTCAAAAAGTAATGACGCTCCCTCCCAACCCACACCTGGCGCGTCTGCTTTCCACCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                                              <  2:7019887/140‑1 (MQ=255)
                                           tCGCTATTACAAAGAAAACATGCATCCGGGGCGTATTAAAACAGTAATGAAGCGCCCTTCCAAAAGACGCCGGGCGAGTCAGCTTTCCACCAGACAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCt                                                                                              <  1:7023699/140‑1 (MQ=255)
                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATc                                                                                         >  1:7545896/1‑140 (MQ=255)
                                                aTTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTGCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACCGCTTGCTGCTGAGGGCtatagc                                                                                         >  2:3906403/1‑138 (MQ=255)
                                                      aataaaaCATGCATCCGGGGGGTATTCAAAAAGGAATTAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCAATCTGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGc                                                                                   <  2:465513/137‑1 (MQ=255)
                                                       aGAAAACATGCAGCCGGGGGTTATTCAAAAATTAATGAAGCCCCCTTCCAAAAGACTCGGGGCGAGTAAGCTTTCCATCAGAAAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCa                                                                                  <  1:103870/140‑1 (MQ=255)
                                                           aaCATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGcc                                                                              >  1:1903067/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCACGAAGCGGATGGTTTg                                                             >  1:3248701/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                             >  2:5608667/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                             >  1:4781080/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                             >  2:344020/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                             >  1:5070483/1‑140 (MQ=255)
                                                                            tATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTg                                                             >  2:3047112/1‑140 (MQ=255)
                                                                                 aaaaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAt                                                        >  2:4607066/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                      >  1:2134934/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                      >  2:2778559/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                      >  2:1700763/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTa                                                      >  1:6701395/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAGTa                                                      >  1:4547923/1‑140 (MQ=255)
                                                                                   aaaGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAAAGAGTGTAGAACTTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGAGTa                                                      >  1:7372258/1‑140 (MQ=255)
                                                                                     aGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATc                                                    <  2:6548352/140‑1 (MQ=255)
                                                                                            acgcccccTTCCAAACGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTc                                             <  1:4674681/135‑1 (MQ=255)
                                                                                             aGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCt                                            >  2:6689756/1‑140 (MQ=255)
                                                                                               cTCCCTTCCAAAAGACTCCTGGAGAGTCAGCTTTCCATCAGTCAATCGACTGTAGAACGTGCCTAGGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGGTTGCTGATTATCATGATTCTGa                                          >  1:6121483/1‑140 (MQ=255)
                                                                                                 cccTTCCAAAAGACTCCGGGCGAGTCAGCTTTCCATCAGTCACACGACTGTAGAACGTGCCTCTGAACAGCTTGCTGCGGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGAGGGTTTGCTGATTCTCATGATTCTGagt                                        >  1:7190714/1‑138 (MQ=255)
                                                                                                  ccTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGAttt                                       >  1:6739749/1‑140 (MQ=255)
                                                                                                      ccAAACCACGCCGGGCGAGTCAGGTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCg                                   <  2:2444636/140‑1 (MQ=255)
                                                                                                               tCCTGGCGAGTCAGCTTTCCATCAGTCAATCGACTGTAGACCGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTGTGATTTTGCGTATCGTaaa                          >  2:2026856/1‑140 (MQ=255)
                                                                                                               tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGGCTATGACCAGCTTGCTGGTGAGGGCGCTAGCGTGAGCCCGCCAAGACGCGGATGGTGTGGTGTTTCTCATGATTCTGATTTTGCGGAGCGTaat                          >  1:6399168/1‑139 (MQ=255)
                                                                                                               tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGACCAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGAGGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaa                          >  2:5448051/1‑140 (MQ=255)
                                                                                                               tCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTaaa                          >  2:4193829/1‑140 (MQ=255)
                                                                                                                ccTGGCGAGTCAGCTTTCAATCAGTCAAACGACTGTAGAACGTGACTAGGAACAGCTGGCTGATGAGGGCTATATCGTGAGCAAGCCAAGAATCTGATGGTTTGATGATTATCATGATTCTGATATTGCGTATCGTaaaa                         >  1:2223635/1‑140 (MQ=255)
                                                                                                                  tGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATa                       >  1:6478553/1‑140 (MQ=255)
                                                                                                                  tGGCGAGTCAGCTTTCCATCAGTAAAACGACTGTAGAACGTGCCTATGAACAGATTGCTGCTGAGGGCTATATAGTCAGCAAGCCAAGAATCGGATTGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATa                       >  1:575920/1‑140 (MQ=255)
                                                                                                                    gcgtttCAGCTTTCCATCAGCAACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATAcc                     <  2:2134934/135‑1 (MQ=255)
                                                                                                                    gCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATAcc                     <  1:344020/140‑1 (MQ=255)
                                                                                                                    gCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATAcc                     <  1:7293849/140‑1 (MQ=255)
                                                                                                                     ctaGTCTGGTTTCCTTCAGTCACACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCa                    <  2:2976941/138‑1 (MQ=255)
                                                                                                                        gTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgc                 >  1:86312/1‑140 (MQ=255)
                                                                                                                         tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgcg                >  2:2729627/1‑140 (MQ=255)
                                                                                                                         tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgcg                >  1:5951807/1‑140 (MQ=255)
                                                                                                                         tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgcg                >  2:7266114/1‑140 (MQ=255)
                                                                                                                         tCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAgcg                >  2:6183186/1‑140 (MQ=255)
                                                                                                                           aGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCa              >  2:2877474/1‑140 (MQ=255)
                                                                                                                                  cATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCaa       >  2:7367680/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCaa  >  2:6787752/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCaa  >  2:7108445/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCaa  >  2:5934976/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCaa  >  1:4013236/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCaa  >  2:5227057/1‑140 (MQ=255)
                                                                                                                                       gTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGGATCGTAAAATACCAAGAGCATCACCAAGTCaa  >  2:4722654/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTTTGAATAGAAATCTGGATATTCCGCTTTATCAGCAGCTTTATCGCTATTTCAAAGAAAACATGCATCAGGGGCGTATTCAAAAAGGAATGAAGCTCCCTTCCAAAAGACTCCTGGCGAGTCAGCTTTCCATCAGTCAAACGACTGTAGAACGTGCCTATGAACAGCTTGCTGCTGAGGGCTATATCGTGAGCAAGCCAAGAAGCGGATGGTTTGCTGATTATCATGATTCTGATTTTGCGTATCGTAAAATACCAAGCGCATCACCAAGTCAA  >  NZ_CP034943/549005‑549279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: