Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,359 A→C 15.2% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,3590AC15.2% 779.0 / 31.6 415T68P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (119/208);  new base C (10/68);  total (134/281)
Fisher's exact test for biased strand distribution p-value = 3.84e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 422 total aligned reads displayed.

GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498
                                                                                                                                          |                                                                                                                                           
 ccACGGGAGCTAGTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCGGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           <  2:2912817/140‑1 (MQ=255)
 ccACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAAcc                                                                                                                                           >  1:6278473/1‑140 (MQ=255)
    cGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGa                                                                                                                                        <  2:4131643/140‑1 (MQ=255)
         gCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACAGGGGAAGCTGGCGCACCCGGACCGACGGGAGCCACAGGACCTACCGGGGAAGCCGGACCAGCGTGAGCGCCCGGAGCGAc                                                                                                                                   >  2:2464142/1‑140 (MQ=18)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  2:6436431/140‑1 (MQ=255)
          cTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACg                                                                                                                                  <  1:1819430/140‑1 (MQ=255)
                  gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  1:422594/140‑1 (MQ=255)
                  gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:592853/140‑1 (MQ=255)
                  gAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGATGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  2:7286469/140‑1 (MQ=255)
                  gAAGCGGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                                                                          <  1:2533518/140‑1 (MQ=255)
                     gCTGGCCCAGCCGGACCCGCCGGAGCGACGGGAGCCTCGGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGCCGGGAGCGACAGGAGGTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:3026151/140‑1 (MQ=21)
                     gCTGGACCAGCCGGACCCGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:401254/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGCCGTGAGCCTCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGATACGGGAGCGACAGGAGGTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  2:1011784/140‑1 (MQ=21)
                     gCTGGACCAGCCGGACCAGCCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:4815811/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:5599889/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacgggagcgacggg                                                                                                                       >  1:1453861/1‑140 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       >  2:4485219/1‑140 (MQ=255)
                     gCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACggg                                                                                                                       <  1:4510468/140‑1 (MQ=255)
                     gCTGGACCAGCCGGACCAGACGGAGCGACCCGATCAACGCGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGACGGGCGCGACAGGAGCTACCGGGGAAGCCGGAGAAGCAGGCCCACCCGGAGCGACGGGGGCGCCggg                                                                                                                       >  1:1670432/1‑140 (MQ=17)
                      cTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGa                                                                                                                      <  1:3609754/140‑1 (MQ=255)
                          accatcaggaccagcaggAGCCACTGGAGCCACGGGAGCTACCGGCGAACCTGGACCAGCCGGAGCGACGGGAGCGACCGGAGCTACAGGGGATGCCGGCCCAGCCGGACCAACCGGTGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:5182303/140‑1 (MQ=17)
                          accagccggaccagccggAGCGACGGTAGCCACGGGAGCTACAGGGGAACCGGGACCAGCCGGAGCCACGGGAGCCACAGGAGCTACGGGGGAAGCCGGACCAGCCGGACCCACCGTATCGACAGGAGCAACCGGAGcaa                                                                                                                  >  1:7204229/1‑138 (MQ=17)
                          accagccggaccagccggAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTa                                                                                                                  <  2:6309966/140‑1 (MQ=255)
                              gccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              >  2:4420409/1‑140 (MQ=255)
                              gccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCgg                                                                                                              >  2:5669008/1‑140 (MQ=255)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAGCCGGAGCGACGGGAGCGACGGGAGCTACCggg                                                                                                             >  2:3752509/1‑140 (MQ=255)
                               ccggaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCAGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGAGAAGCCGGCGCAGCCGGAGCAGCCGGAGCAACCGGCGCAACGAGAGCTCCCggc                                                                                                             >  1:220043/1‑139 (MQ=14)
                                  gaccagccggaGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGaa                                                                                                          <  2:2768501/140‑1 (MQ=255)
                                  gaccagccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGaa                                                                                                          <  2:6924882/140‑1 (MQ=255)
                                   cccccccggaCCGACGGGAGCCACGGGCGCGACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGAAGGGAGCCACGGGAGCTACCGGGGAag                                                                                                         <  1:5037603/135‑1 (MQ=18)
                                       gccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAagccgg                                                                                                     >  2:1537948/1‑140 (MQ=255)
                                        ccggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAGTCCGGTGCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGCTACCGGGGAagccgga                                                                                                    >  1:527534/1‑140 (MQ=18)
                                         cggaGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggac                                                                                                   <  1:5879941/140‑1 (MQ=255)
                                            aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccag                                                                                                <  1:7579549/140‑1 (MQ=255)
                                                aCGGGAGCCACGGGAGCTACCGGGGAACCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgg                                                                                            <  1:3521030/140‑1 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGCGCAACCGGAGCGCCGGGGGCGACGGGAGCTGCCGGCCCagccggagcggccggc                                                                                           >  2:6140067/1‑139 (MQ=14)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCGACGGGAGCTAGCGGGGActccggaccggccgga                                                                                           >  1:3770493/1‑140 (MQ=18)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           >  2:811689/1‑140 (MQ=255)
                                                 cGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccgga                                                                                           >  2:6067055/1‑140 (MQ=255)
                                                  gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGCACCCGGACAAGACGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAagccggacaagacggcc                                                                                          >  1:7472791/1‑138 (MQ=17)
                                                  gggAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccggaccagccggac                                                                                          <  2:1655963/140‑1 (MQ=255)
                                                         actggAGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   <  2:3120536/137‑1 (MQ=255)
                                                         aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                   <  1:1969132/140‑1 (MQ=255)
                                                           gggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                 >  2:7186684/1‑140 (MQ=255)
                                                           gggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                 >  2:7059673/1‑140 (MQ=255)
                                                               gCTACCGGGGAAGCTGGACCAGCCGGATCGAGGGGAGCGACAGGAGCTACCGGGGACGCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                             <  1:2840148/140‑1 (MQ=255)
                                                                cTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                            <  1:6718860/140‑1 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGTCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGTACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAATCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:1559140/140‑1 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:4140957/140‑1 (MQ=255)
                                                                  aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                          <  1:1046832/140‑1 (MQ=255)
                                                                        gAAGCTGGACCAGCCGGAGCGTCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                    <  2:5400201/140‑1 (MQ=255)
                                                                         aaGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgaca                                                                   <  2:2656865/140‑1 (MQ=255)
                                                                         aaGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgaca                                                                   <  2:4828223/140‑1 (MQ=255)
                                                                          aGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGgagcgacaggagcgacag                                                                  <  2:4981634/140‑1 (MQ=255)
                                                                           gCTGGACCAGCCGGAGCGCCGGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 <  1:6114606/140‑1 (MQ=255)
                                                                           gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACGGGTGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGagcgacaggagcgacagg                                                                 <  1:1387110/140‑1 (MQ=255)
                                                                           gCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                 >  2:357492/1‑140 (MQ=255)
                                                                             tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:2259800/140‑1 (MQ=255)
                                                                             tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  1:811689/140‑1 (MQ=255)
                                                                             cggACCAGCCGGAGCGCCGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                               <  2:2051434/139‑1 (MQ=255)
                                                                                aCCAGCCGGCGGGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  1:2404321/140‑1 (MQ=17)
                                                                                aCCAGCCGGAGTGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGCGAAACCGGGGAAGCCGGACCACCCGGAGCAACCGGAGCGACAGGAGCGACACTAGCtc                                                            >  2:6329229/1‑139 (MQ=14)
                                                                                aCCAGCCGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:1120106/140‑1 (MQ=255)
                                                                                aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                            <  2:4057676/140‑1 (MQ=255)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGGCCAACCGGAGCGACAGGAGCGACAGGAGCGACCgg                                                        >  1:2291165/1‑140 (MQ=255)
                                                                                    gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCAACCTGAGCgacaggagccacgggagcgccagg                                                        >  2:3654844/1‑134 (MQ=14)
                                                                                    gCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGCCCGGAGCTCCCgg                                                        >  1:2739423/1‑140 (MQ=255)
                                                                                     cgggggCGACGGGCGCGACGGGAGGTCCCGGTGAAGCCGGACCCGCCGGACCAACGGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:6486194/135‑1 (MQ=14)
                                                                                     ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:7042140/140‑1 (MQ=255)
                                                                                     ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  2:5518864/140‑1 (MQ=255)
                                                                                     ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  2:3939222/140‑1 (MQ=255)
                                                                                     ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  1:1155288/1‑140 (MQ=255)
                                                                                     ccGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:7139328/140‑1 (MQ=255)
                                                                                         aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   <  1:2639642/140‑1 (MQ=255)
                                                                                          gCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGc                                                  <  1:72760/140‑1 (MQ=255)
                                                                                               cggAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                             <  2:4628432/139‑1 (MQ=255)
                                                                                                  aGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAg                                          <  1:5409373/140‑1 (MQ=255)
                                                                                                    ctccAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGTGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  2:3810830/137‑1 (MQ=255)
                                                                                                    ccaccggAGCTACAGGGGCAGCCGGACCAGCCGGACCACCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  1:3357012/135‑1 (MQ=14)
                                                                                                    cGACTGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                        <  1:3931558/140‑1 (MQ=255)
                                                                                                             cTACCGGTGAAGCCGGCGCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGaca                               >  2:3034065‑M2/1‑137 (MQ=255)
                                                                                                             cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGACGCCGGACCAGCCGGACCCACCGGAGCGACAGGAGCGACAGGAGCTAGCGGGGACGCCGGCCCAGCCGGAGCGaca                               <  2:1770129‑M2/140‑4 (MQ=255)
                                                                                                               aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACAGGAGCGACCGGAGCGACCGGGGCTAGCGGTGAAGCCGGAGCGGCCGGAGCGagggg                             <  2:1472259‑M2/140‑6 (MQ=255)
                                                                                                                 cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGCACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGacaggag                           <  2:6602392‑M2/140‑8 (MQ=255)
                                                                                                                    ggAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcga                        <  2:7473204/140‑1 (MQ=255)
                                                                                                                     gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAagccgga                                                                                                    >  2:6501578/1‑63 (MQ=255)
                                                                                                                      aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  1:4420409/140‑1 (MQ=255)
                                                                                                                      aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                      <  1:5669008/140‑1 (MQ=255)
                                                                                                                       agccggaccagccggaccAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCCGCCggagcgacaggagcgacag                     >  1:4974575/1‑140 (MQ=255)
                                                                                                                        gccggagctgccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:5797014/140‑1 (MQ=17)
                                                                                                                        gccggaccagccggaccaGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                    <  2:6736949/140‑1 (MQ=255)
                                                                                                                         ccggtgaagccggaccaCCCGGAGCGACTGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagga                   <  2:42468/133‑1 (MQ=17)
                                                                                                                              gcagcctgacctCCCGCTGCGACTCGCGCCACCGGAGCTACCGGGGCAGCAGGACCAGCCGGACCACCCGGAGCCAGAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  2:913507/139‑1 (MQ=17)
                                                                                                                              ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  1:1282385/140‑1 (MQ=255)
                                                                                                                              ccagccggaccaACCGGCGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac              <  2:3275085/140‑1 (MQ=255)
                                                                                                                                cgccggaccaCCCGGCGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCCCCGGAGCGACTGGAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGgagcgacaggagcgacaggagcgacag            <  2:2612035/139‑1 (MQ=14)
                                                                                                                                 gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgacaggagcgacaggagcgacagg           <  1:5130988/140‑1 (MQ=255)
                                                                                                                                  ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          >  2:1256061/1‑140 (MQ=255)
                                                                                                                                  ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga          >  2:2102533/1‑140 (MQ=255)
                                                                                                                                          aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg  >  2:4192164/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
GCCACGGGAGCTACTGGGGAAGCTGGACCAGCCGGACCAGCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607221‑607498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: