Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,413 A→C 6.3% T86P (ACC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,4130AC 6.3% 912.3 / 4.5 396T86P (ACC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base A (133/214);  new base C (4/45);  total (137/259)
Fisher's exact test for biased strand distribution p-value = 9.18e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

CCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607276‑607552
                                                                                                                                         |                                                                                                                                           
  aCGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCgg                                                                                                                                         <  2:6425280/140‑1 (MQ=255)
    tggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                                                                       <  1:7268809/139‑1 (MQ=255)
    gggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggag                                                                                                                                       >  2:7186684/1‑140 (MQ=255)
     ggAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGACGGGAGCCACGGGAGCTCGCGGGGAAGCCGGACCCGCCGGACCAACCggagc                                                                                                                                      >  2:6506341/1‑140 (MQ=255)
       aGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcga                                                                                                                                    <  1:200031/140‑1 (MQ=255)
        gCTACCGGGGAAGCTGGACCAGCCGGATCGAGGGGAGCGACAGGAGCTACCGGGGACGCCGGACAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgac                                                                                                                                   <  1:2840148/140‑1 (MQ=255)
         ctcccGGGGAAGCTGGACCAGCCGGCGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                                                                  <  2:6308875/137‑1 (MQ=255)
         cTACCGGGGACGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgaca                                                                                                                                  <  2:2099319/140‑1 (MQ=255)
           aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGTGCGACAGGCGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacagg                                                                                                                                <  1:4853413/140‑1 (MQ=255)
           aCCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCGACCGGAGCGACGGGAGCCACGGGAGCGACCGGGGAAGCCGGAGCAGCCGGAGCAACAggagcgaccgg                                                                                                                                >  2:1829622/1‑140 (MQ=17)
             cGGGGAAGCTGGACCAGCGGGAGCGACGGTAGCGACTGGAGCGACCGGGGAAGCCGGACCTGCGGGACCAACCGGAGCGACGGGAGCCACGGGAGCTAGCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggag                                                                                                                              <  2:288064/140‑1 (MQ=18)
                cgatccTGGACCAGCCGGAGCGCCGGGAGGGACGGGAGCTACCGGTGCACCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcga                                                                                                                           <  2:975744/135‑1 (MQ=17)
                 gAAGCTGGACCAGCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgac                                                                                                                          <  2:4821190/140‑1 (MQ=255)
                  aaGCTGGACCAGCCGGAGCGTCGGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgaca                                                                                                                         <  2:1596772/140‑1 (MQ=255)
                  aaGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCggagcgacaggagcgaca                                                                                                                         <  2:2656865/140‑1 (MQ=255)
                    tcTGGACCATCCTGAGCGACGGGAGCGAGAGGAGCTACCGGGGAAGCCTGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGTGAAGCCGGACCAGCCGGACCAACCGGagcgacaggagcgacagg                                                                                                                       <  2:4068749/139‑1 (MQ=17)
                      tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     >  2:3991946/1‑140 (MQ=255)
                      tGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     <  1:811689/140‑1 (MQ=255)
                      tGGACCACCCGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGcgacaggagcgacaggag                                                                                                                     <  2:2051805/140‑1 (MQ=255)
                         aCCAGCCGGCGGGACGGGAGCGACCGGAGCTACCGGGGAAGCCGGCCCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                  <  1:2404321/140‑1 (MQ=17)
                         aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                  <  1:6932849/140‑1 (MQ=255)
                         aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGAGACAGGAGCGACAGGAGCTa                                                                                                                  >  2:2977219/1‑140 (MQ=255)
                         aCCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTa                                                                                                                  <  1:447672/140‑1 (MQ=255)
                             gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGTCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCgg                                                                                                              >  1:5562437/1‑140 (MQ=255)
                             gCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGGCCAACCGGAGCGACAGGAGCGACAGGAGCGACCgg                                                                                                              >  1:2291165/1‑140 (MQ=255)
                             gCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCgg                                                                                                              >  1:2739695/1‑140 (MQ=255)
                              cgggggCGACGGGCGCGACGGGAGGTCCCGGTGAAGCCGGACCCGCCGGACCAACGGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  1:6486194/135‑1 (MQ=14)
                              cccgAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCTGACAAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGATACCGGGGAAGCCGGACCAGCCGGACCTACCGGAGCGACAGGAGCGACAGCAGCTACCggg                                                                                                             >  1:1123705/4‑140 (MQ=14)
                              ccGGCGCGCCGGGTGCGACAGGAGCGACCGGGGAACCCGGCCCAGCCGGACCAACCGGAGCGCCGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCggg                                                                                                             <  1:1790934/140‑1 (MQ=17)
                              ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  2:4974001/140‑1 (MQ=255)
                              ccGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGTGACGCCGGACCAGCCGGTCCAACCGGCGCGACCGGAGCGACAGGAGCTCCCggg                                                                                                             >  1:7613654/1‑140 (MQ=17)
                                       cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:851167/140‑1 (MQ=255)
                                        gggAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGCAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  2:2483761/140‑1 (MQ=255)
                                             cGACTGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                              <  1:3931558/140‑1 (MQ=255)
                                             cGACAGGAGCTACCGGGGAAGCCGGACCAGTCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                              <  2:5562437/140‑1 (MQ=255)
                                             cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCCCGGGAGCTACCGGGGAAGCCGGACCACCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGCAAGCCGGACCAGcc                                                                                              >  2:6685313/1‑140 (MQ=255)
                                             cGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACTGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                              <  1:1627644/140‑1 (MQ=255)
                                               accggAGCTACCGGCGAAGCCGGACCCGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCCGCCGGACCACCGGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:5082524/137‑1 (MQ=14)
                                               aCAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:5703172/140‑1 (MQ=255)
                                                 aGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGACCGACGGGAGCCACCGGAGCTACCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggag                                                                                          <  2:5926660/140‑1 (MQ=255)
                                                  ggAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCACCGGGACCAACCGGACCGACAGGAGAGACAGGAGCACCCGGGGCAGCAGGCCCAGCCggggc                                                                                         >  2:1090472/1‑140 (MQ=14)
                                                      ctcccGGGGATGCAGGACCAGCCGGACCACCCGGCGCGACGGCAGCCACGGGCGCTACCGGGGAAGCCGGCCCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgaca                                                                                     <  2:1713746/137‑1 (MQ=17)
                                                      cTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCAACCGGCCCCGCCGGCCCAGCCTGACCCACCGGCGCTACAGGAGAGACTGTAGCGACCCCACAAGCCGCACCAGGCgtagcgaca                                                                                     >  2:4917633/1‑140 (MQ=9)
                                                          cGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGacaggag                                                                                 <  2:4338147‑M2/140‑8 (MQ=255)
                                                              gAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgac                                                                             >  2:3646861/1‑140 (MQ=255)
                                                               aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            >  2:6570683/1‑140 (MQ=255)
                                                               aaGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgaca                                                                            >  2:2726697/1‑140 (MQ=255)
                                                                agccggaccagccggaccAACCGGAGCGACGGGAGCCACCGGCGCTACCGGGGACGCCGGACCAGCCGGAGCCACCGGGGCTCGAGGAGCGCCAGGCGCTCCGGGGGCTGCGGGCCCAGCCggagcgacaggagcggcgg                                                                           >  1:4610458/1‑136 (MQ=9)
                                                                 gccggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacagg                                                                          >  1:3637104/1‑140 (MQ=255)
                                                                 gccggaccagccggaccaACCGGAGCGACGGGAGCCACCGGAGCTCCCGGGGAAGCCGGTCCACCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTGCAGGGGAATCAGGACCAGCCGGACCGacaggagcgaccgg                                                                          <  1:7140391‑M2/140‑15 (MQ=255)
                                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGGCGGACCATCAGGAGCAACCGGAGCGAAAGGAGCCAAAGGAGCTACCGGTGAAGAAGGAGCAGCCggagctacaggagaaacaggag                                                                        >  2:4178646/1‑140 (MQ=14)
                                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCggagcgacaggagcgacaggag                                                                        >  2:4234476/1‑140 (MQ=255)
                                                                   cggaccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        >  1:6233286/1‑140 (MQ=255)
                                                                   cggaccacccggaccaccCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggag                                                                        <  2:3566466/140‑1 (MQ=255)
                                                                       gcagccggacctCGCGTAGCGACGGGAGCCACTGGAGCTACCGGGCAAGCCGGACCAGCCGGACCCCCGGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  2:7565487/139‑1 (MQ=14)
                                                                       ccagccggaccaCCCGGAGCGACGGGAGCCACGGGAGCTCCCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:1282385/140‑1 (MQ=255)
                                                                       ccagccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCTACAGGAGCTACCGGGGAAGCCGGACCAGCCggagcgacaggagcgacaggagcgac                                                                    <  1:2504080/140‑1 (MQ=255)
                                                                         cgccggaccaCCCGGACGGACGGGAGCCACCGGCGCTACCGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGgagcgacaggagcgacaggagcgacag                                                                  <  2:3808412/139‑1 (MQ=14)
                                                                          gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCCGCCGGagcgactggagcgacaggagcgacagg                                                                 >  2:5240906/1‑140 (MQ=255)
                                                                          gccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGagcgaccggagcgacaggagcgacagg                                                                 >  2:7310622/1‑140 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                >  2:3161113/1‑140 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGgagcgacaggagcgacaggagcgacagca                                                                >  1:4011753/1‑138 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                <  2:146445/140‑1 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                <  2:1352946/140‑1 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                >  2:1256061/1‑140 (MQ=255)
                                                                           ccggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAgcgacaggagcgacaggagcgacagga                                                                >  1:137305/1‑140 (MQ=255)
                                                                            cggaccaACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacaggagcgacaggagcgacaggag                                                               <  2:4439557/140‑1 (MQ=255)
                                                                              gaccaGCCGGGGCGACCGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCt                                                             <  1:3423506/140‑1 (MQ=14)
                                                                                   aCCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCgg                                                        >  2:7045718/1‑140 (MQ=255)
                                                                                    ccGGAGCGACGGGAGCCACGGGAGCTACGGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       <  1:4994609/140‑1 (MQ=255)
                                                                                    ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCggg                                                       >  1:7374371/1‑140 (MQ=255)
                                                                                    ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACAGGAGCGACAGGAGCTACCggg                                                       <  1:3556727/140‑1 (MQ=255)
                                                                                    ccGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCGACCggg                                                       >  1:6714508/1‑140 (MQ=255)
                                                                                        aGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAg                                                   >  1:7097065/1‑140 (MQ=255)
                                                                                         gCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGTGACAGGAGCTACCGGGGAAGc                                                  >  1:4129072/1‑140 (MQ=255)
                                                                                         gCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGc                                                  >  1:3789840/1‑140 (MQ=255)
                                                                                                  gCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGacaggagctaccggggaagccgg                                                                 <  2:5748177‑M2/116‑24 (MQ=255)
                                                                                                       gggAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                    >  1:5270948/1‑140 (MQ=255)
                                                                                                          aGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAACCAGGACCAGCGGGAGcca                                 >  2:6009653/1‑138 (MQ=255)
                                                                                                              acgggggAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:5910327/137‑1 (MQ=255)
                                                                                                              aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCggg                                                                                                             <  2:3880518/60‑1 (MQ=255)
                                                                                                              aCCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGTCGCCACCGCATCGCCCGGCCCACCCGGCCAACCGGGAGCAGGTGGAGCGACggg                             >  1:1431117/1‑140 (MQ=9)
                                                                                                                 ggggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             <  1:325080/137‑1 (MQ=255)
                                                                                                                 ggggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             >  2:325080/1‑137 (MQ=255)
                                                                                                                   ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                             >  1:7293733/1‑135 (MQ=255)
                                                                                                                   ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                        <  2:1155288/140‑1 (MQ=255)
                                                                                                                    gcagcgggaccagccggaccACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  2:4551692/138‑1 (MQ=255)
                                                                                                                    gAAGCCGTTCGAGCTGTAGCACCCGGCGCGATAGGAGAGACATGAGATACCGGGGAAGACTGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:4143925/140‑1 (MQ=17)
                                                                                                                    gAAGCCGGACCTGGCGGACCAACCGGCGGGACTTGAGAGACAGGACCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:1007336/140‑1 (MQ=17)
                                                                                                                    gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:6056111/140‑1 (MQ=255)
                                                                                                                    gAAGCCGGACCAGCCGGACCAACCGCAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                       <  1:2652738/140‑1 (MQ=255)
                                                                                                                     aaGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACa                      >  1:6874190/1‑140 (MQ=255)
                                                                                                                       gccggcccagccggaccaACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                    <  2:2121831/140‑1 (MQ=255)
                                                                                                                       gccggaccagccggaccaACCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAgg                    <  1:576327/140‑1 (MQ=255)
                                                                                                                       gccggaccagccggaccaACCGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCGACAGGAGCGACAGGAGCGACCGGGGCAGCTGGACCAGCAGGAGCGCCGGGcgcgcccgg                    >  2:6109450/1‑135 (MQ=17)
                                                                                                                       gccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACCGGAGCGAGCGGAGCCCCAGGAGCTACCGGGGCGCCGGGAGCGGCGGGAGCGACCGGAGCggccgg                    >  2:6267320/1‑135 (MQ=14)
                                                                                                                        ccggacccgcggcaccaCCGGGAGCGACCGCAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTTCAGGAGCGAAAGGAGCGACAGTAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                   <  2:5483541/140‑1 (MQ=14)
                                                                                                                                 ccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCggg          >  1:6389856/1‑140 (MQ=255)
                                                                                                                                         aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGCCAGGAGCAACCGGGGCAGCTGGACCAGCTGGAGCGACGGGCGCGACCGGGGCGACCGGGGCAGCCgg  >  1:4279067/1‑140 (MQ=18)
                                                                                                                                         aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCgg  >  1:297929/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CCACGGGAGCTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACGGGAGCCACGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGG  >  NZ_CP034943/607276‑607552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑