Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,514 T→G 5.1% A119A (GCT→GCG)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,5140TG 5.1% 811.3 / 3.0 356A119A (GCT→GCG) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base T (122/181);  new base G (13/19);  total (151/205)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

CGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607378‑607652
                                                                                                                                        |                                                                                                                                          
 gggAGCTACCGGGGACGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAg                                                                                                                                        >  1:5270948/1‑140 (MQ=255)
 gggAGCTACCGGGGAAGCCGGACCAGCCGGACCCACCGGAGCGCCAGGAGCGACAGGAGCTACCGGCGAAGCCGGAGCACCCGGAGCGCCAGGAGCGCCCGGAGCGACCGGAGCGACCGGGGACGCAGCACCACCGGGAg                                                                                                                                        >  2:403968/1‑140 (MQ=14)
        acgggggAAGCCGGACCAGCCGGACCACCCGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  1:5910327/137‑1 (MQ=255)
        aCCGGGGAACCCGGACCAGCCGGACCAACCGGAGCGACTGGAGCGAGTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 <  2:4967885/140‑1 (MQ=14)
          cGGGGATGCCGGACCCGCCCGACCACCCCGAGCGACAGGAGCCACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCTACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                                                                                                               <  2:6272332/140‑1 (MQ=11)
          cGGGGAAGCCGGACCAGCCGGACCACCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAg                                                                                                                               <  2:6278473/140‑1 (MQ=255)
           ggggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACggg                                                                                                                                 >  2:4351431/1‑137 (MQ=255)
             ggAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGa                                                                                                                            <  2:1155288/140‑1 (MQ=255)
              gacgccggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:320558/137‑1 (MQ=255)
              gAAGCCGTTCGAGCTGTAGCACCCGGCGCGATAGGAGAGACATGAGATACCGGGGAAGACTGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:4143925/140‑1 (MQ=17)
              gAAGCCGGACCTGGCGGACCAACCGGCGGGACTTGAGAGACAGGACCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:1007336/140‑1 (MQ=17)
              gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:2340786/140‑1 (MQ=255)
              gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  1:6056111/140‑1 (MQ=255)
              gAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGAc                                                                                                                           <  2:1678452/140‑1 (MQ=255)
               aaGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACa                                                                                                                          >  1:6874190/1‑140 (MQ=255)
               aaGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACa                                                                                                                          >  1:3011583/1‑140 (MQ=255)
                agccggaccagccggaccCACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACGGGAGCGACAg                                                                                                                         >  1:6895396/1‑140 (MQ=255)
                 gccggaccagccggaccaACCGGAGCGACAGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCGGGACCCGCCGGAGCGACGGGCGCGACCgg                                                                                                                        >  2:1946846/1‑140 (MQ=11)
                  ccggaccagccggaccaACCGGAGCGACTGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGa                                                                                                                       <  2:5601132/140‑1 (MQ=255)
                   cggaccagccggaccaACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAg                                                                                                                      >  2:4193671/1‑140 (MQ=255)
                   cgcacccgccgccccaCCCGGACCGAGCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCAGCCGGAGCCTCAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAg                                                                                                                      <  2:2643567/137‑1 (MQ=14)
                   cgcaccagccgcaccaCCCGGAGCGACAGGACCCACCGGAGCAACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGCGAAGCTGTACCAGCGGGAGCGACGGGAGCGACAGGAg                                                                                                                      <  2:3174054/137‑1 (MQ=11)
                      cccagccggaccaCCCGGTGCGACAGGAGCCACTGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                                                                                                   <  1:6436014/139‑1 (MQ=14)
                      accagccggaccaCCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTa                                                                                                                   <  1:2247138/140‑1 (MQ=255)
                           ccggaccaCCCGGAGCGCCAGGAGCCACAGGAGCTACGGGTGTAGCCGGACCAGCCGGACCGCCCGGAGCGACTGGAGCGACAGGAGCTACCGGTGAAGCTGGACGAGCTGGAGCCACGGGAGCCACAGGAGCTACCggg                                                                                                              <  2:2194113/140‑1 (MQ=14)
                               accaccCGGAGCGACAGGAGCGCCCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg                                                                                                          <  1:4028693/140‑1 (MQ=255)
                                   aCCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCgg                                                                                                      >  1:1392627/1‑140 (MQ=255)
                                    ccGGAGCGACAGGAGCGACAGGAGCTCCCGGGGAAGCCGGCCCACCCGGACCGACAGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                     <  2:6529174/140‑1 (MQ=17)
                                    ccGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                     <  1:5707246/140‑1 (MQ=255)
                                    ccGGAGCGAATCGACGGACACGAGCTACCGGGTTAGCCGGCTCAGCCGGGGCGACCGGAGCCACACGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                     <  1:6140501/140‑1 (MQ=17)
                                     cGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                    <  2:4923956/140‑1 (MQ=255)
                                          cgacaggagcgacaggagGTCCCGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCCACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                               <  1:1117554/140‑1 (MQ=17)
                                          cgacaggagcgacaggagCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGcc                                                                                               <  1:3544034/140‑1 (MQ=255)
                                            acaggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGCGCTACCGGGGAACCTCGCCAAGCTGGTGCGACGGGAGCCACAGGCGCGACCGGTGCCGCCGGAGCAGCCgg                                                                                             >  1:976472/1‑140 (MQ=17)
                                            acaggagcgacaggagcTACAGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACAGGCGCGACAGGTCCAACCGGCGAAGCTGGAGCCACAGGAGCTACGGGAGAGCCCGGCGGGACCGGCGACCCCGGACCACCCgg                                                                                             >  1:3334138/1‑140 (MQ=12)
                                             caggagcgacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCggg                                                                                            >  2:4783274/1‑140 (MQ=255)
                                                   cgacaggagaTACAGGGGCAGCCGGACCAGGCGGAGCGACTGGAGCGACAGGAGCGACAGGTGCTAACGGGTAAGCTGGACCTGCTGGAGCGACTGGAGCGACAGGTGCTACCGGGGAAGCCGGACCAGCCGGGGCCagg                                                                                      <  2:7131424/140‑3 (MQ=18)
                                                   ccacaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACg                                                                                      <  2:2694758/138‑1 (MQ=255)
                                                     tcaggagcTAGCGGGGAAGCCGGACCAGACGGAGCGACAGGAGCGACAGGAGCGACAGTAGATTCCGGGGTAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGTAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:6602392/139‑1 (MQ=18)
                                                     acaggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    <  1:1256061/140‑1 (MQ=255)
                                                       aggagcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAg                                                                                  <  1:2726697/140‑1 (MQ=255)
                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAGAGGAGCGACAGGAGCAACCGGGGACGCTGGACCACCTGGAGAGACGGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGAGCcac                                                                              >  1:3955003/1‑140 (MQ=18)
                                                           gcTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              >  1:6450916/1‑140 (MQ=255)
                                                           gcTACCGGGGAAGCCAGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              >  1:4277228/1‑140 (MQ=255)
                                                            ctcccGGCGAAGCCGCACCAGCCGGAGCCACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  1:5950477/137‑1 (MQ=255)
                                                            ccaCCGGGGAAGCCGGCCCCCCCGGCGCCACCGGAGCGACCGGCGCGACAGGCGCTACCGGCGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcaca                                                                             <  2:1458713/138‑1 (MQ=17)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGGGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCTGGGGCGGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGGCCAGCGGGGGCGACGGGAGCCACAgg                                                                           >  1:7472746/1‑140 (MQ=21)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACAGGAGCGACAGGAGCTACCGGCGAAGCAGGACCAGCTGGAGCGACGGGAGCGACAGGAGCGACCGGGGACGCCGGACCAGCCGGGGCGACGGGCGCCCCAgg                                                                           >  2:730889/1‑140 (MQ=18)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGACGCTGGACCAGCTGGAGCGACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           >  2:1756426/1‑140 (MQ=255)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGCGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           >  1:2128754/1‑140 (MQ=255)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACggg                                                                                    >  2:2114886/1‑131 (MQ=255)
                                                              aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgg                                                                           <  1:4193671/140‑1 (MQ=255)
                                                                cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGCGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAgggg                                                                         <  1:4685736/140‑1 (MQ=255)
                                                                   ggcctcaggACCACCCGGAGCGCCAGGAGCTACAGGACCTACAGGACCTCCCGGGCAAGCAGGACGCGCGGGAGCCACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  2:894171/133‑1 (MQ=18)
                                                                   ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGTGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:2102533/140‑1 (MQ=255)
                                                                   ggAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:1607179/140‑1 (MQ=255)
                                                                   cccacccGGACCAGCCGGAGCGACCGGCGCCCCTGGAGCGACCTGAGCTACCGGCGAAGCTGGACCAGCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTa                                                                      <  1:2464142/135‑1 (MQ=18)
                                                                    gAAGCCGGACCAGCCGGAGCGACAGGATCGACAGGAGCGACAGGAGCTCCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGACGGGGCCACGGGCGCCACCGGGGcgcc                                                                     >  2:3878651/1‑137 (MQ=18)
                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTCCCGGGGACGCCGGACCACCCGGCGCCACCGGAGCCCCAGGCGCGAc                                                                     >  2:3983587/1‑140 (MQ=18)
                                                                     aaGCCGGACCAGCCGGAGCGACAGGAGCGACCGGAGCGACAGGAGCGCCCGCCGACGCTGGACCAGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGcggcg                                                                    >  1:791859/1‑136 (MQ=21)
                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACCGGAGCTACCGGACACGCCGGACCAGCCGGGGCCACCGTAGCCCCCGGGGCTACggg                                                                  >  1:6723058/1‑140 (MQ=18)
                                                                       gCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              <  1:2826595/128‑1 (MQ=255)
                                                                        ccGGACCCCCCGGAGCCCCAGGACCGCCAGGAGCGACAGGCGCCCCCGGCCAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACgggg                                                                 <  2:161008/140‑1 (MQ=18)
                                                                         cGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACCGGAGCTACCGGGGCAGGTGGACCACGGGGAGCGACGGGAGCGAAAGGAGCTACGGGGGCAGGCGGAGCGGCGGGGGCGACGGGAGCCACGGGGGGGGCTgggg                                                                >  2:3378473/1‑140 (MQ=12)
                                                                         cGGACCAGCCGGAGCGACAGGAGAGACAGGAGCGACCGGAGATACCGGTGAACCTGGACCAGCTGGAGCGACGGGAGAGACAGGAGCTACCGGGGAAGCCGGACCAGACGGTGCCACGGGAGAAACAGGGGCGACGgggg                                                                >  2:1397703/1‑140 (MQ=18)
                                                                            cccccccGGAGCCACCGCAGCGCCAGGCCCGACAGCCGCTCCCGGCGACCCCCGCCCAGCTGGAGCGACGGGCGCGACTGGAGCTACCGCGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                             <  1:1470210/135‑1 (MQ=12)
                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                             >  1:5395111/1‑140 (MQ=255)
                                                                            aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                             >  1:7074538/1‑140 (MQ=255)
                                                                                 ccGGAGCGACAGGAGCGACCGGAGCCACAGGCCCACCCGGCGCAGCGTGAGCAGAGGCAGCGACGGGATCGACCGGGGCTCGAGGCCAAGACGGACCCTGCGGCGCCACGGGAGCGAGTGGGGCTCCGGGGGCGGCCggg                                                        >  2:4583955/1‑139 (MQ=255)
                                                                                 ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                        <  2:7374371/140‑1 (MQ=255)
                                                                                 ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                        <  2:3493554/140‑1 (MQ=255)
                                                                                     agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCCGCCGGACCAg                                                    >  2:5655614/1‑140 (MQ=255)
                                                                                      gcgacaggagcgacaggagcgactggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                   <  2:3011583/140‑1 (MQ=255)
                                                                                      gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                   >  1:7195890/1‑140 (MQ=255)
                                                                                         tccggagcgacaggagcgagaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                <  2:3789840/137‑1 (MQ=255)
                                                                                           aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCGTGGGGAAGCCGGACCAGCCGGAg                                              >  2:2499352/1‑140 (MQ=255)
                                                                                           aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                              >  2:662898/1‑140 (MQ=255)
                                                                                               gcgacagtagcgaaagtcgcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                          <  1:6329229/140‑1 (MQ=255)
                                                                                               gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              >  1:704244/1‑104 (MQ=255)
                                                                                               gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                >  1:4535135/1‑134 (MQ=255)
                                                                                               gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                <  2:4535135/134‑1 (MQ=255)
                                                                                                cgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACa                                         >  1:5045538/1‑140 (MQ=255)
                                                                                                  acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                       >  1:6632955/1‑140 (MQ=255)
                                                                                                  acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                       <  2:701388/140‑1 (MQ=255)
                                                                                                    aggagcgacaggagcTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                     <  1:1770254/140‑1 (MQ=255)
                                                                                                       agcgacaggagcTACCGGGGAAGCGGGACCAGCTGGAGCGTCTGGAGCGCCAGGAGCAACCGGGGAAGCCGGAGCAGGCGGGGCCGCGGGAGCGACAGGGGCTACGGGGGACGCAGGACCGGCCGGAGCGACCGGAGCtc                                  >  1:6529801/1‑139 (MQ=21)
                                                                                                           acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAg                                                                                                          <  1:4888972/64‑1 (MQ=40)
                                                                                                           acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGACCtcgcgg                              >  1:4746248/1‑135 (MQ=21)
                                                                                                             aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                            >  1:1723419/1‑140 (MQ=255)
                                                                                                                     ccGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                    <  2:6109528/140‑1 (MQ=255)
                                                                                                                     ccGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCCGCCGGAGAGACAGGAGCTACCGGGGAAGCCGGa                    >  2:5417928/1‑140 (MQ=255)
                                                                                                                       ggggAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                  >  1:3147866/1‑140 (MQ=255)
                                                                                                                          gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGCCGGGAGGTAGCCGGGACGCCGGACCAGc               >  2:2354572/1‑140 (MQ=18)
                                                                                                                             gCTGGACCCGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg            <  1:4344325/140‑1 (MQ=255)
                                                                                                                             gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCcac                                                                              <  1:7310794/74‑1 (MQ=255)
                                                                                                                              caggACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGa           <  1:3446556/138‑1 (MQ=255)
                                                                                                                                  aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGa       >  1:3879340/1‑140 (MQ=255)
                                                                                                                                  aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa       >  1:1852160/1‑140 (MQ=255)
                                                                                                                                       cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGTAGCCACACCAGCTACCGGGGCAGCCGGACCCGCCGGCGCGACAGGa  >  1:461514/1‑140 (MQ=17)
                                                                                                                                       cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa  >  2:129522/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
CGGGAGCTACCGGGGAAGCCGGACCAGCCGGACCAACCGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGA  >  NZ_CP034943/607378‑607652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑