Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 68,857 G→T 5.1% intergenic (+16/‑2) yabQ → / → divIC spore cortex biosynthesis protein YabQ/cell division protein DivIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP03494368,8570GT 5.1% 1425.1 / 8.1 547intergenic (+16/‑2)yabQ/divICspore cortex biosynthesis protein YabQ/cell division protein DivIC
Reads supporting (aligned to +/- strand):  ref base G (264/229);  new base T (2/52);  total (266/281)
Fisher's exact test for biased strand distribution p-value = 3.21e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 549 total aligned reads displayed.

CTTCCTGTAAAGATGCGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGTATTC  >  NZ_CP034943/68719‑68996
                                                                                                                                          |                                                                                                                                           
       tAAAGATGCGTCTAACTTTTAGACGTTATTTTGAAAAAGTTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTTGTAACCATAATAACTACCATTACACGTTTTTTGAAGAGATGAAAGGATGACAGTCTGGTTTGAAtt                                                                                                                                     >  1:4493476/1‑140 (MQ=255)
               cGTCTAACTTTTAGACGTTATTTTGAAAAATGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCaga                                                                                                                             >  1:596873/1‑140 (MQ=255)
               cGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCaga                                                                                                                             >  1:3934926/1‑140 (MQ=255)
               cGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCaga                                                                                                                             >  2:2261499/1‑140 (MQ=255)
               cGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCaga                                                                                                                             >  2:3709678/1‑140 (MQ=255)
               cGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGAACGTCTGGTTTGAATTTTTCCaga                                                                                                                             >  2:862292/1‑140 (MQ=255)
                gTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCagag                                                                                                                            >  1:4373333/1‑140 (MQ=255)
                 tCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAgaga                                                                                                                           >  1:525513/1‑140 (MQ=255)
                 tCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAgaga                                                                                                                           >  1:477486/1‑140 (MQ=255)
                   tAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGATAACCATAAGAACGACCATCACACGGTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGGgaac                                                                                                                         >  2:4862903/1‑140 (MQ=255)
                    aaCTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAgaacg                                                                                                                        <  1:1772439/140‑1 (MQ=255)
                      cTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTCCACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAgaacgaa                                                                                                                      >  2:4061256/1‑140 (MQ=255)
                         ttAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAGCGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAacgaacga                                                                                                                   >  1:7626042/1‑140 (MQ=255)
                         ttAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAacgaacga                                                                                                                   >  1:1011232/1‑140 (MQ=255)
                         ttAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTGTGAATTTTTCCAGAGAacgaccga                                                                                                                   >  1:3976449/1‑140 (MQ=255)
                          tAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGAt                                                                                                                  <  1:2528910/140‑1 (MQ=255)
                           aGACGTTATTTTGAAAAAGGTGCAGGATTTGTCAAAAAGAAGAAGAACCTATTGGTAACCATAAGAACGACCATTCCACGTTTTTTGAAGAGGTGAAAGGCGGACCGTCTGGTTTGAATTTTTCCTGAGAACGAACGATa                                                                                                                 >  2:5182521/1‑140 (MQ=255)
                               gTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTg                                                                                                             >  2:1854280/1‑140 (MQ=255)
                                ttATTTTGAAAAATGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACAATAACACGTTTTTTGAAGAGATGAAAGGAGGACCGTGTGGTTTGAATTTTTCCAGATAACGAACGATAACTGa                                                                                                            <  1:5664323/140‑1 (MQ=255)
                                 tATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGaa                                                                                                           >  1:5577250/1‑140 (MQ=255)
                                  aTTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACCATAACTGaaa                                                                                                          <  1:2745331/140‑1 (MQ=255)
                                            agttGCAGGATTTCTAAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTCACGATATGAAAGTAGGACCGTGTTGTTTGAATTTTTCCCGAGAACGAACGATAACTGAAATACAAAACGa                                                                                                <  1:4894328/137‑1 (MQ=255)
                                                gCAGTATTTATCAAAAAGAATAAGAATCTATTGTTAACAATAAGAACGACAATTACACTTTTTTTGAATATATTAAAGTAGGACCGTCTGTTTTTAATTTTTCCAGTGAACGAACGATAACTGAAATACAAAACGACtat                                                                                            <  2:6642771/140‑1 (MQ=255)
                                                  aGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAAGGAACGATAACTGAAATACAAAACGACTATaa                                                                                          <  2:3808596/140‑1 (MQ=255)
                                                  aGGATTTACCATCAAGAAGAATAAACTATTGGTAACCATAAGAACGACCATTCCCCGTTTTTTGAATAGAGGAAAGGAGGACCGTCTGGTTTGAATTTTTCCACACAACGAACGATAACTGAAATACAAAACGACTATaa                                                                                          <  2:7089060/140‑1 (MQ=255)
                                                       ttCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAAc                                                                                     >  2:4667302/1‑140 (MQ=255)
                                                        tctcAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGTACCGTCTGTTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACa                                                                                    >  1:7166670/1‑140 (MQ=255)
                                                         ctcAAAAAGAAGAAGAAACTATTGGTAACCATACGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATaactgtctc                                                                                   >  1:1394632/1‑133 (MQ=255)
                                                         ctcAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACaa                                                                                   >  2:2638981/1‑140 (MQ=255)
                                                         ctcAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACaa                                                                                   >  1:1223842/1‑140 (MQ=255)
                                                          acAAAAAGAAGAAGAAACTTTTGGTAACCATAAGAACGACCATTAAACGTTTTTTTAAGACATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAg                                                                                  <  2:5104454/139‑1 (MQ=255)
                                                                           cTATTGGTAACCATTAGATCGACCATTACAGGTTTTTTGAAGATATGAAAGTAGGACCGTCTGTTTTGAATTTTTCCAGTGAACGAACTATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAg                                                                 <  1:5088669/140‑1 (MQ=255)
                                                                            tATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAAAGACTATAAAGAACAATTAGAGCGGCAGAATCAGc                                                                >  1:4262839/1‑140 (MQ=255)
                                                                                        tAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGaagaaaagaa                                                    >  1:5470846/1‑140 (MQ=255)
                                                                                        tAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGaagaaaagaa                                                    >  2:7168444/1‑140 (MQ=255)
                                                                                         aataaCTACAATTACACGTTTTTTGAAGAGATGAAAGTAGTACCGTCTGTTTTTAATTTTTCCAGACAACGAACGATACGTGAATTACAAAACGAATATAAAGAACAAGTAGAGCGGCAGAATCAGCTGaagaaaagacg                                                   <  2:656892/137‑3 (MQ=255)
                                                                                         aaGAACTACAATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAaagaag                                                   <  2:1251198/140‑1 (MQ=255)
                                                                                         aaGAACGACCTTTACAGGTTTTTTGAAGATATGAAAGGAGGACCGTGTGGTTTGAATTTTTCAAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAaagaag                                                   <  2:7615277/140‑1 (MQ=255)
                                                                                         aaGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAaagaag                                                   <  2:4152214/140‑1 (MQ=255)
                                                                                              ctaCCATTACACTTTTTTTGAAGAGATGAAAGGAGGACCTTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  2:6173422/138‑1 (MQ=255)
                                                                                              cGACCATTACACTTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGCACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  2:2723051/140‑1 (MQ=255)
                                                                                              cGACCATTACACGTTTTTTTAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  2:1706880/140‑1 (MQ=255)
                                                                                              cGACCATTACACGTTTTTTGCAGATATGAACGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  1:2629850/140‑1 (MQ=255)
                                                                                              cGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTTAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  2:6903416/140‑1 (MQ=255)
                                                                                              cGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCa                                              <  1:1742959/140‑1 (MQ=255)
                                                                                              cGAACATTACTCTTTTTTTGAAGCCTTCAAAGTTGGACGGTCTTGTTTGAATTTTTACAGTGAATGAACGATAACTGAAATACAAAACTACTATACAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACTCa                                              <  2:4029374/140‑1 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAACAGAAGACGCaaa                                            >  1:6232915/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  1:7415308/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:2360846/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:4527043/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  1:3694997/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  1:2196458/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:1464748/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:7261112/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:1126191/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:5503434/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:5874661/1‑140 (MQ=255)
                                                                                                aCCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCaaa                                            >  2:6279925/1‑140 (MQ=255)
                                                                                                 caaTTACACGTTTTTTTAATATATGAAAGGAGGACCGTCTGTTTTGAATTTTTCCAGAGAACGAACTATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAg                                           <  1:1293187/138‑1 (MQ=255)
                                                                                                   tttACACTTTTTTTGAAGTGATGAAAGGCGGACCGTCTGGTTTGAATTTTTCCAGAGACCGAACGATAACTGAAATACAAAACGACTATAAAGATCAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAggg                                         <  1:3778308/139‑1 (MQ=255)
                                                                                                   aTTACACTTTTTTTGAAGATATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAggg                                         <  2:3598601/140‑1 (MQ=255)
                                                                                                   aTTACACGTTTTTTGAAGAGATTAAAGGAGGACCGTCTGGTTTGAATTTTTCCCGAGAACCAACGATAACTGAAATAAAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAggg                                         <  1:3210996/140‑1 (MQ=255)
                                                                                                   aTTACACGTTTTTTGAAGAGATGAAAGGAGGACCTTCTGGTTTGAATTTTTCAAGAGAACGAACTATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAggg                                         <  2:1994401/140‑1 (MQ=255)
                                                                                                   aTTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAggg                                         <  2:6010213/140‑1 (MQ=255)
                                                                                                         cGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACTATAACTCAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTAc                                   <  1:2394604/140‑1 (MQ=255)
                                                                                                         agTTTTTTGAAGAGATGAAAGGAGGACCGTCTGTTTTGAATTCTACCAGAGACCCCCCGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTAc                                   <  1:5793941/139‑1 (MQ=255)
                                                                                                            tttttGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGa                                >  1:6985434/1‑140 (MQ=255)
                                                                                                            tttttGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGa                                >  1:728478/1‑140 (MQ=255)
                                                                                                            tttttGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGa                                >  2:5701141/1‑140 (MQ=255)
                                                                                                                tGAAGAGATGAAAGGAGGACCTTCTGTTTTTAATTTTGCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  1:308948/140‑1 (MQ=255)
                                                                                                                tGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  2:1047506/140‑1 (MQ=255)
                                                                                                                tGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  2:3297049/140‑1 (MQ=255)
                                                                                                                tGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  2:2497021/140‑1 (MQ=255)
                                                                                                                tGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  2:4269938/140‑1 (MQ=255)
                                                                                                                tGAAGAGATGAAAGCAGCACCGTGTGGTTTGAATTTTGCCAGAGAACGAACCATCACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGt                            <  1:6770836/140‑1 (MQ=255)
                                                                                                                 gAAGAGATGAAAGGAGGACCGTCTGGTTTTAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAACAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGtt                           <  2:3226998/140‑1 (MQ=255)
                                                                                                                             ggcgcaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAACACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCgg               <  1:922325/135‑1 (MQ=255)
                                                                                                                             ggaggaCCGTCTGTTTTGAATTTTTCCAGAGAACGAACCATAACTTAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCgg               <  1:4092952/140‑1 (MQ=255)
                                                                                                                             ggaggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCgg               <  2:1575983/140‑1 (MQ=255)
                                                                                                                             ggaggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCgg               <  2:5577250/140‑1 (MQ=255)
                                                                                                                             ggaggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCgg               <  2:3145993/140‑1 (MQ=255)
                                                                                                                               aggcCCGTCGGTTTTGAATTTTTCCCGCCAACGAACGATAACTGAAATAAAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcg             <  1:656889/140‑1 (MQ=255)
                                                                                                                               aggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACTATAACTTAAATAAAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcg             <  1:6330284/140‑1 (MQ=255)
                                                                                                                               aggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcg             <  1:3497777/140‑1 (MQ=255)
                                                                                                                               aggaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcg             <  2:6476192/140‑1 (MQ=255)
                                                                                                                               aggaCAGTATGTTTTGAATTTTTCCAGATAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcg             <  2:6849618/140‑1 (MQ=255)
                                                                                                                                ccaCCGTCTGGTTTGAACTTTGCCAGAGAACGACCGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGgcgc            <  2:4381540/138‑1 (MQ=255)
                                                                                                                                 gaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGcc           >  2:3449016/1‑140 (MQ=255)
                                                                                                                                 gaCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGcc           >  1:1575423/1‑140 (MQ=255)
                                                                                                                                 gaCCGTCTGGTTTGAATTTTTCCAGAGAACGAAAGATAACTGAAATACAAAACGACTATAAAGAACACGTAGAGCGGCAGAATCAGCTGAATTAAATAAGACGAAAAGTGATGTACAGACGGTTGACTGTCTTCGGCGcc           >  2:5303900/1‑140 (MQ=255)
                                                                                                                                  aCCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGccc          >  1:5521318/1‑140 (MQ=255)
                                                                                                                                    cGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTa        <  2:2326805/140‑1 (MQ=255)
                                                                                                                                     gTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAg       >  1:3330912/1‑140 (MQ=255)
                                                                                                                                      tCTGGTTTGAATTTTTCCAGAGAACGACCGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACg                              <  2:7410950/116‑1 (MQ=255)
                                                                                                                                      tCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAACACGTCTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGt      >  1:1960737/1‑140 (MQ=255)
                                                                                                                                      tCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGTAAAGAAGAGGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCGCTAGt      >  2:2966787/1‑140 (MQ=255)
                                                                                                                                      tCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGt      >  2:5800287/1‑140 (MQ=255)
                                                                                                                                      tCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGt      >  2:4972833/1‑140 (MQ=255)
                                                                                                                                        tGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCGCTAGTAt    >  2:5034040/1‑140 (MQ=255)
                                                                                                                                        tGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGTAt    >  2:1349707/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                           
CTTCCTGTAAAGATGCGTCTAACTTTTAGACGTTATTTTGAAAAAGGTGCAGGATTTCTCAAAAAGAAGAAGAAACTATTGGTAACCATAAGAACGACCATTACACGTTTTTTGAAGAGATGAAAGGAGGACCGTCTGGTTTGAATTTTTCCAGAGAACGAACGATAACTGAAATACAAAACGACTATAAAGAACAAGTAGAGCGGCAGAATCAGCTGAAGAAAAGAAGACGCAAAGGGCTGTACAGACGGTTGACTGTATTCGGCGCCCTAGTATTC  >  NZ_CP034943/68719‑68996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: