BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,592	A→C	13.5%	E145D (GAA→GAC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,592	0	A	C	13.5%	584.9 / 56.8	310	E145D (GAA→GAC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base A (139/122); new base C (21/27); total (161/149)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

ACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACC  >  NZ_CP034943/607454‑607730

aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                                                           >  1:5395111/1‑140 (MQ=255)

aCCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAg                                                                                                                                           <  1:4192164/140‑1 (MQ=255)

     ccGGAGCGACAGGAGCGACAGGAGCGACAGGATCTACCGGTGAAGCATGACCAGCCGGAGCGACGGGACCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGGGCGACGGGAGCGCCGGGGGCTGCGGGGGAcggcggg                                                                                                                                      >  1:314876/1‑139 (MQ=17)

     ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                                                      <  2:7374371/140‑1 (MQ=255)

      ctgagagacaggagcaaaaggagctgcagGAGCAAAAGGTCAAGATGGACTGCCTGGAGTCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACCGGAGCCACTGGGGCTACTGGGGAAGCCGGAc                                                                                                                                     <  1:5355707/138‑1 (MQ=9)

      cggggaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                                                                                                     <  2:2340691‑M1/34‑1 (MQ=255)

         agcgacaggagcgacaggagcgacaggAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCCGCCGGACCAg                                                                                                                                  >  2:5655614/1‑140 (MQ=255)

          gcgacaggagcgacaggagcgccaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGAACGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGc                                                                                                                                 >  1:3086524/1‑140 (MQ=21)

          gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                                                                                 <  2:6874393/140‑1 (MQ=255)

          gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                                                                                                 <  2:4680130‑M1/38‑1 (MQ=255)

          gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccgcagcgACGGGAGCCACAGGGGCTACAGGGGCAGTCGGACCAGc                                                                                                                                 <  2:6014494‑M1/38‑1 (MQ=255)

           cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                                                                <  2:1756025/140‑1 (MQ=255)

           cgacaggagcgacaggagcgacaggagCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGcc                                                                                                                                <  2:4558650/140‑1 (MQ=255)

             gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggacgccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg                                                                                                                              <  1:49548‑M1/41‑1 (MQ=255)

             gccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACTGGAGCCACAGGGGCTACTGTGGACGTCGGACCAGCCgg                                                                                                                              <  1:3826922‑M1/41‑1 (MQ=255)

              caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGa                                                                                                                             >  2:1625376/1‑140 (MQ=255)

              caggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGGCGCACTGGGCACGCCGGCCCTGCCgcc                                                                                                                             >  2:3920969/1‑138 (MQ=18)

              caggagcgacaggagcgacaggagcTACCGGGGAAGCGGGACCATCAGGAGCGACGGGAGCCACCGGAGCAAGATGCGAAGCGGGACCTGCCGGCGCCTCTGGAGCGGCAGGTGAGAGTGGGGCCGCCGGACAAGCCGGa                                                                                                                             >  2:2685758/1‑140 (MQ=9)

               aggagcgacaggagcgacaggagcTCCCGGGGAAGCCGGACCACCTGGAGCGACGGGAGCGACTGGAGCTACCGGGGAAGCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                            <  2:3038926/140‑1 (MQ=21)

               aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACGGGGGAAGCCGGACCAGCCGGAg                                                                                                                            >  2:5615020/1‑140 (MQ=255)

               aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGATACCGGGGCAGCCGGACCAGCCGGGCCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGCGCAGCCGGAg                                                                                                                            >  2:2048313/1‑140 (MQ=18)

                  agcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                         <  1:7059673/140‑1 (MQ=255)

                   gcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                              >  1:4535135/1‑134 (MQ=255)

                   ccacccggagcgacgggagccacaggggctactggggaagccggaccagccggagcgacaggagctaccggggaagccggaccacccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                                                                                                        >  1:6707452‑M1/94‑140 (MQ=255)

                    cgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGGGCCACGGGAGCGACAGGGGCAGCGGGGGACGCCGGAGCAGCCGGAGCGACa                                                                                                                       >  2:3808959/1‑140 (MQ=18)

                      accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg                                                                                                                     <  1:770974‑M1/50‑1 (MQ=255)

                      acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                     >  1:2413037/1‑140 (MQ=255)

                        cggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                   <  1:1770129/139‑1 (MQ=255)

                        aggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGACACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGAGACAGGAg                                                                                                                   >  1:3368148/1‑140 (MQ=255)

                        aggagcgacaggagcTACCGGGGAAGCGGGACCAGCTGGAGCGACGGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGACACAGGGGCTACGGGGGAAGCCGGACCAGCCGGCGCGACCGGAg                                                                                                                   >  1:2181924/1‑140 (MQ=21)

                           agcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggacgccggaccaaccggagcgCCGGGAGCCACCGGGGCGACTGGGGCCGGCGGAGCAGCCGGAGcgccaggcgcga                                                                                                                <  2:6808465‑M1/55‑6 (MQ=255)

                           agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                >  1:1024281/1‑140 (MQ=255)

                               acaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGTGCCACGGGAGCCACAGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGACAGGACCtcgcgg                                                                                                            >  1:4746248/1‑135 (MQ=21)

                                ccggagcCCCCGGCCAAGCTGGCCCAGCTGGAGCGACGGGAGCGACCGGAGCCACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                           <  2:424441/138‑1 (MQ=18)

                                   gagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGaa                                                                                                        >  1:6106511‑M1/78‑140 (MQ=255)

                                    agcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGacg                                                                                                       >  2:3242382/1‑138 (MQ=255)

                                        aCCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGGGCTACCGGGGAAGCCGGACCAGCCGGCGCCCCGGGTGCGACAGGGGCCACCGGCGCAGCCGGACCCGCCGGTGCTACAGCACCGACCggggccgcacg                                                                                                   >  2:5155453/1‑133 (MQ=12)

                                         ccGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:6109528/140‑1 (MQ=255)

                                         ccGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCCGCCGGAGAGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  >  2:5417928/1‑140 (MQ=255)

                                              gctactggggaagccggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACCGGAGCCACAGGGGCAACAGGCCCACCCGGACCAGCCGGAGCGACAGGCGCTGACGGGGAAGCCGGAccaac                                                                                             <  2:6488315‑M1/74‑3 (MQ=255)

                                                 gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                          <  2:416709/140‑1 (MQ=255)

                                                 gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                          >  1:7173587/1‑140 (MQ=255)

                                                      ccccgCTGGAGCGTCCGGAGCCACCGGACCAACCGGCCCACCCGGACCAGCCGGCGCAACGGGAGCCACAGGAGCTACCGGCGTAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                     <  1:6524429/136‑1 (MQ=14)

                                                      cccAGGTGGAGGCACGGGGGCGACTGGAGCTCCCGGGGCTCCCGGACCACCCGGCGCCACCGGACCACCAGGTGCACCTGGCCAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                     <  1:5762145/139‑1 (MQ=9)

                                                       ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                    <  2:297929/140‑1 (MQ=255)

                                                        ccgcgggAGCCACGGGAGCGACAGGAGCTCCGGGGGACGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                   <  2:6615621/135‑1 (MQ=14)

                                                           cTGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCGACTGGGGAAGCCGGACCAGCGGGAGCGTCAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGa                                                                                >  1:1954985/1‑140 (MQ=17)

                                                           cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                >  2:252135/1‑140 (MQ=255)

                                                               aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGcga                                                                            >  2:343676/1‑138 (MQ=255)

                                                               aGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                            <  1:1625376/140‑1 (MQ=255)

                                                                   aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                        <  1:3472741/140‑1 (MQ=255)

                                                                    cGGGAGCCACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGGGCCCCGGGAGCCCGAGTGGCTTCTGGGGAAGCGGGACCACCCGGAGCTACAGGAGCTCCCGGTGAAGCCGGAGCCGCGGGAGCGACAGGAGCTACCggg                                                                       <  1:1393939/140‑1 (MQ=18)

                                                                     gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                      >  2:1173270/1‑140 (MQ=255)

                                                                     gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGCTGAAGCCGGACCAGCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCAGCCGGAGCCACAGGAGCTACCgggg                                                                      >  2:4382522/1‑140 (MQ=255)

                                                                             cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                              <  1:129522/140‑1 (MQ=255)

                                                                                   cgcccGGGGAACCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                        <  1:730889/137‑1 (MQ=255)

                                                                                   cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                        >  1:6878238/1‑140 (MQ=255)

                                                                                   cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAAcc                                                        >  1:63449/1‑140 (MQ=255)

                                                                                     aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                                                     >  2:3692536/1‑61 (MQ=255)

                                                                                     aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCGGGAACaggcgg                                                      >  1:296352/1‑135 (MQ=17)

                                                                                     aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACGAACCgg                                                      >  2:5295672/1‑140 (MQ=255)

                                                                                     aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGAGAAGCCGGACCACCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCgg                                                      >  1:3908815/1‑140 (MQ=18)

                                                                                       cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                    <  2:1852160/140‑1 (MQ=255)

                                                                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCAACCGGGGACGCCGGCCCGACCGGAGCGAc                                                >  2:7170113/1‑140 (MQ=18)

                                                                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                           >  2:5488930/1‑113 (MQ=255)

                                                                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                <  2:6905225/140‑1 (MQ=255)

                                                                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                >  1:5511442/1‑140 (MQ=255)

                                                                                           gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACTGGAGCGACCGGGGCTGCcggagcgaccggagcgac                                                >  1:4860072/1‑140 (MQ=17)

                                                                                              gCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGATGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAgg                                             <  1:1473228/140‑1 (MQ=17)

                                                                                              gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                  <  1:2342749/119‑1 (MQ=255)

                                                                                                cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                           <  2:6381808/140‑1 (MQ=255)

                                                                                                cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                           <  2:7074538/140‑1 (MQ=255)

                                                                                                   aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGGGCCCCCGGACCAACAGCAGCGGCAGGAGcga                                        >  1:2881359/1‑138 (MQ=17)

                                                                                                        ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                   >  1:1858539/1‑140 (MQ=255)

                                                                                                        ccGGCGCCCCCGGAGCCACAGCCGCTACGGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                   <  1:1174681/140‑1 (MQ=11)

                                                                                                                       ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                  <  2:199847/62‑1 (MQ=40)

                                                                                                                       ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCACCCGGACCAACCGGAGCGCCGGGAGCGACCGGGGCAGCCGGACCAGcc                    >  1:2537489/1‑140 (MQ=14)

                                                                                                                        cacaGGCGCTACTGGGGAAGCCGGACCAGCCGGACCCAGCGGAGCTTCCGGCGACCCGGGAGCTGCCGGAGACACAGGAGCACCCGGCCAAGCCGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCg                   <  1:879421/140‑1 (MQ=2)

                                                                                                                         ccaGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAGCCgg                  <  1:5615020/139‑1 (MQ=255)

                                                                                                                         acaGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCgg                  <  1:993328/140‑1 (MQ=255)

                                                                                                                         acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                  <  1:662898/140‑1 (MQ=255)

                                                                                                                           aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGCAGCTCCCGGGGACGCCGGACCAGCCGGAg                >  1:6319291/1‑140 (MQ=255)

                                                                                                                             gggCGACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg              <  1:371191/140‑1 (MQ=255)

                                                                                                                              ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGACCGa             >  2:4691690/1‑140 (MQ=255)

                                                                                                                              ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa             >  2:6682106/1‑140 (MQ=40)

                                                                                                                                 tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                       <  2:2151481/47‑1 (MQ=38)

                                                                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACggg                                                                                 >  1:6912351/1‑68 (MQ=25)

                                                                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg         >  2:1316649/1‑140 (MQ=255)

                                                                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg         >  2:4950671/1‑140 (MQ=255)

                                                                                                                                  aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCACAGGACCAGCAGCAGCggggtt         >  2:618266/1‑135 (MQ=11)

                                                                                                                                   cgggtgcaGCGGGACCAGCCGGAGCGACAGGAGCTCCCGGGGCCCCCGGAGCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa        <  1:352352/133‑1 (MQ=11)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGCGACGCCGGACCAGCCGGAGCGACAGGAGCtcc   >  1:6390991/1‑138 (MQ=255)

                                                                                                                                        gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGGGAGGAGCCCCCGGCGACGCCGGACCAACCGGAGCTCCATTAGCAAACGGCGAAGCCGGACCACCACGCGCGAGAGGAGATAc   >  1:2764024/1‑140 (MQ=2)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGCCCGGGGACGCCGGACCAGCCGGAGCGCCAGGAGCTAcc  >  2:2454833/1‑140 (MQ=14)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGACGCCGGTCCAGCCGGCGCGTCCGGAGCGACCGGGGACGCCGGCGCAGCCGGAGCGCCCGGAGCTAcc  >  1:4677822/1‑140 (MQ=11)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCTAGAGGAGcagcc  >  1:2605581/1‑136 (MQ=14)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                   >  1:3666302/1‑75 (MQ=34)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGTCAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACGGTAGCGAcc  >  1:214080/1‑140 (MQ=11)

                                                                                                                                         aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc  >  2:1453721/1‑140 (MQ=255)

                                                                                                                                         aaGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCGACAGGAGCTAca  >  1:1688571/1‑139 (MQ=255)

ACCAGCCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACC  >  NZ_CP034943/607454‑607730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`