BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,594	C→T	9.9%	A146V (GCC→GTC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,594	0	C	T	9.9%	803.0 / 63.1	329	A146V (GCC→GTC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base C (168/123); new base T (7/24); total (179/150)
Fisher's exact test for biased strand distribution p-value = 2.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

CCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607459‑607732

ccGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGa                                                                                                                                        <  2:7374371/140‑1 (MQ=255)

 cggggaagccggaccaaccggagcgacgggagccacaggcgcgactggggacgtcggaccagccggagcgaaagtagctaccggggaagccggaccaaccggagccCCGGGAGCCACAGGGGCTCCGGGGGACGTCGGAc                                                                                                                                       <  1:6109737‑M1/34‑1 (MQ=255)

     gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                                                                                   <  1:4078067/140‑1 (MQ=255)

     gcgacaggagcgacaggagcgacaggaGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGc                                                                                                                                   <  2:6874393/140‑1 (MQ=255)

     gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGc                                                                                                                                   <  1:4994577‑M1/38‑1 (MQ=255)

     gaagccggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccgcagcgACGGGAGCCACAGGGGCTACAGGGGCAGTCGGACCAGc                                                                                                                                   <  2:6014494‑M1/38‑1 (MQ=255)

       atccggacccaccggagcgcccggagccacaggtgctactggggccctcggtcacgccggagcgacaggacctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCg                                                                                                                                 >  2:1972068‑M1/101‑140 (MQ=255)

        tccggagcgacaggagcgagaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCgg                                                                                                                                <  2:3789840/137‑1 (MQ=255)

        cccggaccaaccggagccacgggagccacaggggctactggggacgtcggcccagccgcagcgacaggagctaccggggatgccggaccatccggagcgACGGGAGCCACAGGGGCTACGGGGGACGCCGGACCAGCCgg                                                                                                                                >  1:4613767‑M1/100‑140 (MQ=255)

         ccggaccaaccggagccacgggagccacaggggcgactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGa                                                                                                                               >  2:5511442‑M1/99‑140 (MQ=255)

          cggcgcgaccggagcgacaggcccCCCCGGCGCAGCGGGACCACCTGGAGCGACGGGAGCGACCGGAGATCCCGGGGAAGCCGGACCAGCCGGGGCGACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAg                                                                                                                              <  2:3028596/136‑1 (MQ=17)

          cggaccaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGCCGCGACTGGGCACGTCGCACCAGCCGGAg                                                                                                                              <  2:4427769‑M1/43‑1 (MQ=255)

          aggagcgacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCGTGGGGAAGCCGGACCAGCCGGAg                                                                                                                              >  2:2499352/1‑140 (MQ=255)

             accaaccggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa                                                                                                                           <  2:5417965‑M1/46‑1 (MQ=255)

              gcgacagtagcgaaagtcgcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:6329229/140‑1 (MQ=255)

              gccacaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                          <  1:2977219/137‑1 (MQ=255)

                gaccggagcgaccggcgcGCCCGGACAAGCTGGACCACCCGGAGCGACCGGAGCGACAGGCGCTTCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAg                                                                                                                        <  2:1452906/140‑1 (MQ=18)

                 ccaggaccgacaggcgcTCCAGGTCTACCTGGACCGGCGGGAGCCACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCTGCCGGGGCCACGGGAGCCACAGGGGATACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  1:1635675/139‑1 (MQ=17)

                 accggagcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccgcggaagccggaccaaccggagcgACGGGAGCCACAGGGGCTACTGGGGCCGTCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:5023504‑M1/50‑1 (MQ=255)

                 acaggcgcgacaggctcTACCGGGGAAGCTGGACCAGCGGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       <  2:5618812/140‑1 (MQ=255)

                 acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGGCGGACCAGCCGGGGCGACCGGAGCGCCCGGGGCTACAGGGCACGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:7194571/1‑140 (MQ=18)

                 acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:6632955/1‑140 (MQ=255)

                 acaggagcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                       >  1:3541219/1‑140 (MQ=255)

                   aggagcgacaggagcTACCGGGGAAGCTGGACCAGCCGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                     <  1:1770254/140‑1 (MQ=255)

                   agctgcgacaggagcTACCGGTGAACCGGGACCATCTGCAGCGACGGTAGCTACAGGAGCTACCGGGGAACCCGGACCAGCCGGCGCCAGGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACAGGAg                                                                                                                     <  2:4011753/136‑1 (MQ=18)

                      agcgacgggagccacaggggctactggggacgtcggaccagccggagcgacaggagctaccggggacgccggaccaaccggagcgCCGGGAGCCACCGGGGCGACTGGGGCCGGCGGAGCAGCCGGAGcgccaggcgcga                                                                                                                  <  2:6808465‑M1/55‑6 (MQ=255)

                      agcgacaggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  >  1:1024281/1‑140 (MQ=255)

                           caggcgcTACCGGTGAAGCCGGAACAGCGGGAGCGACGGGATCGACCGGATCTAACGGGGAAGCCGGACCAGCCGGGGCCACGGGATCCACAGGGGATTCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  2:2146413/140‑1 (MQ=18)

                            aggagcTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                                                            >  1:1723419/1‑140 (MQ=255)

                                   aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCGACTGGGGAAGCCGGACCAGCCGGAGCGCCAGGCGCTACCGGGGACGCCgg                                                                                                     >  1:5552566/1‑140 (MQ=21)

                                   aCCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCgg                                                                                                     >  1:3087810/1‑140 (MQ=255)

                                    ccGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGCGCGACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    <  2:6109528/140‑1 (MQ=255)

                                     cGGCGAAGCCGCACCCGCTGGAGCGCCGGGAGCGACAGGAGCTCCCGGTGAAGCCGGACCACCCGGCGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAc                                                                                                   <  2:1831552/140‑1 (MQ=17)

                                         gctactggggaagccggaccagccggagcgacaggagctaccggggaagccggaccaaccggagcgACCGGAGCCACAGGGGCAACAGGCCCACCCGGACCAGCCGGAGCGACAGGCGCTGACGGGGAAGCCGGAccaac                                                                                               <  2:6488315‑M1/74‑3 (MQ=255)

                                         gAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCTGGGGACGCCGGACCAGCCGGAGCGCCGGGAGGTAGCCGGGACGCCGGACCAGc                                                                                               >  2:2354572/1‑140 (MQ=18)

                                            gCTGGACCCGCTGGAGCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:4344325/140‑1 (MQ=255)

                                            ccTGGACCAGCAGGAGCGCCGGGAGCCACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:3423284/139‑1 (MQ=18)

                                                 aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                       >  1:6882772/1‑140 (MQ=255)

                                                   ccgcgggAGCCACGGGAGCGACAGGAGCTCCGGGGGACGCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                     <  2:6615621/135‑1 (MQ=14)

                                                     gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                   >  1:2807351/1‑140 (MQ=255)

                                                     gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                   <  1:4707792/140‑1 (MQ=255)

                                                     ccTGGAGCGACGGGAGCGACAGGTGAGACCGGGGCAGCCGGACCCGCCGGAGCCACGGGAGCCACAGGCGCTACAGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAGCCGGAGCGACAgg                                                                                   <  1:1495706/139‑1 (MQ=17)

                                                      cTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa                                                                                  >  2:129522/1‑140 (MQ=255)

                                                               cgggctcGACAGGAGAGACCGGTGAAGCCGGTCCAGCCGGCGCCCCGGGAGCAACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                         <  1:4897796/134‑1 (MQ=17)

                                                               cGGGAGCCACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGGGCCCCGGGAGCCCGAGTGGCTTCTGGGGAAGCGGGACCACCCGGAGCTACAGGAGCTCCCGGTGAAGCCGGAGCCGCGGGAGCGACAGGAGCTACCggg                                                                         <  1:1393939/140‑1 (MQ=18)

                                                                gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAc                                                                             >  2:2286926/1‑135 (MQ=255)

                                                                   aGCGACAGGCGCTACCGGGGAACCCGGAGCAGCCGGGGCCACGGGAGCCACAGGCGCTACTGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  1:2905645/140‑1 (MQ=17)

                                                                   aGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  2:4656222/140‑1 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                <  1:129522/140‑1 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                <  1:2397946/140‑1 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                <  2:3147866/140‑1 (MQ=255)

                                                                              cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGcc                                                          >  1:2628606/1‑140 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGAc                                                                                                                                       <  1:3692536/61‑1 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGcc                                                                               >  2:4686487/1‑116 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                        >  1:7159241/1‑140 (MQ=255)

                                                                                    gggAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGGAGCCGGACCAACCGGAGCg                                                    >  1:3476647/1‑140 (MQ=255)

                                                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACCGGAGCAACCGGGGACGCCGGCCCGACCGGAGCGAc                                                  >  2:7170113/1‑140 (MQ=18)

                                                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACGGGAGCCAc                                                                             <  1:5488930/113‑1 (MQ=255)

                                                                                      gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                  <  2:6905225/140‑1 (MQ=255)

                                                                                      cccgCCGGCCCAGCCGGGGCCACGGGAGCCACCGGGGCGACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGAc                                                  <  1:5370718/137‑1 (MQ=17)

                                                                                         gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAgg                                               >  1:7535584/1‑140 (MQ=255)

                                                                                         gCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGATACCGGGGCAGCAGCACCAAGACGAGCCACAgt                                               >  1:994662/1‑139 (MQ=17)

                                                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                             <  2:6381808/140‑1 (MQ=255)

                                                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                             <  2:5395111/140‑1 (MQ=255)

                                                                                           cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                             <  2:7074538/140‑1 (MQ=255)

                                                                                              cccAGCCGGGGCCTCCGGTGCCACAGGGGCTTCTGGGGAAGCCGGAGCAGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                          <  1:1887722/139‑1 (MQ=17)

                                                                                              aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCACCCGCAGCGGCGGGAGccg                                          >  2:172997/1‑138 (MQ=17)

                                                                                                   ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCggg                                     >  1:2266750/1‑140 (MQ=255)

                                                                                                   ccGGCGCCCCCGGAGCCACAGCCGCTACGGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                     <  1:1174681/140‑1 (MQ=11)

                                                                                                   acGGCGCCACCGGAGCCCCAGGCGCTCCAGGTGACCCCGGACCCCCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                     <  1:851428/139‑1 (MQ=14)

                                                                                                       ggCCCCGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:6009653/140‑1 (MQ=255)

                                                                                                       ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                 <  1:6370668/140‑1 (MQ=255)

                                                                                                        gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCAcgg                                       >  1:654154/1‑131 (MQ=255)

                                                                                                        gCCACGCGAGCCACAGGGGAGACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTCAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                <  1:3878651/140‑1 (MQ=14)

                                                                                                             gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      >  1:6938836/1‑81 (MQ=18)

                                                                                                             gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      <  2:2852741/81‑1 (MQ=18)

                                                                                                                  ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                    >  1:199847/1‑62 (MQ=40)

                                                                                                                  ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCCGcc                      >  2:4225629/1‑140 (MQ=255)

                                                                                                                    accggggCTACCGGGGAAGCCGGACCCGCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGACCGACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                    <  1:2499352/137‑1 (MQ=11)

                                                                                                                    acaGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                    <  1:3747018/140‑1 (MQ=255)

                                                                                                                      aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGCAGCCACAGGGGCAACGGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAg                  >  1:652127/1‑140 (MQ=14)

                                                                                                                       ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGCGCTACCGGGGACGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGACCCCGGGCCGGCCGGAGc                 >  2:2644621/1‑140 (MQ=14)

                                                                                                                        gcgCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                <  1:6729298/138‑1 (MQ=255)

                                                                                                                         ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCaac                                                                                               <  1:3243093/60‑3 (MQ=18)

                                                                                                                         ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa               >  2:6682106/1‑140 (MQ=40)

                                                                                                                          gCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc              <  2:3884587/140‑1 (MQ=255)

                                                                                                                          gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGCCCCCGGACCACCCGGACCGAc              >  2:3379128/1‑140 (MQ=11)

                                                                                                                            tACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         >  1:2151481/1‑47 (MQ=38)

                                                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGAGCCACAGGACCAGCAGCAGCggggtt           >  2:618266/1‑135 (MQ=11)

                                                                                                                             aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACC‑GGGAAGCCCGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGa          >  1:4816711/1‑140 (MQ=14)

                                                                                                                               tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                             <  1:993221/104‑1 (MQ=38)

                                                                                                                                  ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGcga      >  2:1538868/1‑138 (MQ=255)

                                                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc    >  1:2558169/1‑140 (MQ=255)

                                                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  2:3569760/75‑1 (MQ=34)

                                                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc    >  2:4325488/1‑140 (MQ=38)

                                                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACAAGCCGGAGCGACAGGAGCGAcc    >  2:4850454/1‑140 (MQ=255)

                                                                                                                                    aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAACCCGGGCCACCCGGAGCGACAGGAGCAACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGAcc    >  2:2033886/1‑140 (MQ=11)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                       >  2:5656570/1‑55 (MQ=35)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                       <  1:5656570/55‑1 (MQ=35)

                                                                                                                                      gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCCCCCCCGGAGCGACAGGAGCGACAGGGGAAGCCGGGCCAGCCGGCGCGACAGGAGCTACCgg  >  2:524043/1‑140 (MQ=14)

CCGGAGCGACAGGAGCGACAGGAGCGACAGGAGCTACCGGGGAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGG  >  NZ_CP034943/607459‑607732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`