BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,638	G→C	8.1%	A161P (GCC→CCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,638	0	G	C	8.1%	616.8 / 5.2	295	A161P (GCC→CCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base G (143/108); new base C (9/16); total (164/131)
Fisher's exact test for biased strand distribution p-value = 5.72e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 300 total aligned reads displayed.

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607500‑607777

   gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         <  2:416709/140‑1 (MQ=255)

   gCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                         >  1:7173587/1‑140 (MQ=255)

        ccccgCTGGAGCGTCCGGAGCCACCGGACCAACCGGCCCACCCGGACCAGCCGGCGCAACGGGAGCCACAGGAGCTACCGGCGTAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    <  1:6524429/136‑1 (MQ=14)

        aCCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                                                                    >  1:6882772/1‑140 (MQ=255)

         ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  2:1392627/140‑1 (MQ=255)

         ccAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  2:297929/140‑1 (MQ=255)

         ccAGCGGGAGCCCCGGCAGCGACAGCAGCTTCCTTGGAAGCCGGACCACCCGGGGCCTCGTGAGCCACAGCTTCTACTGGTGAAGCCGGAGCAGCCGGAGCGACTGTAGCTACCGGTGAAGCCGGACCAGCCGGAGCGAc                                                                                                                                   <  2:4286426/140‑1 (MQ=16)

            gCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGACGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                                >  1:2807351/1‑140 (MQ=255)

            gCTGGAGCGACGGGAGCGACAGGAGCTACCGGCGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                                                                <  1:1643439/140‑1 (MQ=255)

             caggAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCGACGGGGGAAACCGGAGCAGCCGGAGCCGCCGGAGCGGCAGGAGCTACCGGGGACGCCGGAgcgaccggagcgaccgga                                                                                                                               >  1:3772377/3‑140 (MQ=2)

             cTGGAGCGACGGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACCGGGGCGACTGGGGAAGCCGGACCAGCGGGAGCGTCAGGAGCTACCGGGGAAGCCGGAGCTGCCGGAGCGACAGGa                                                                                                                               >  1:1954985/1‑140 (MQ=17)

              tctcgCGACGGGAGCGACCGGAGCTACCGGGGCATCCGGACCAGCCGGGGCCACGGGAGCCACAGGTGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAg                                                                                                                              <  1:6140067/136‑1 (MQ=17)

                     aCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgg                                                                                                                       <  1:3472741/140‑1 (MQ=255)

                      cgggctcGACAGGAGAGACCGGTGAAGCCGGTCCAGCCGGCGCCCCGGGAGCAACAGGCGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:4897796/134‑1 (MQ=17)

                      cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGGGAAGCCGGACCAGCCGCAGCGACAGGAGCTACGGGGGAAGCCGGAGCACCCGGAGCGACAGGAGCTACCggg                                                                                                                      >  2:6110433/1‑140 (MQ=17)

                      cGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTCCGGGCGAAGCCGGAGCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGGGCGAGAGGAGCGACgggg                                                                                                                      >  2:4680742/1‑140 (MQ=14)

                      cGGGAGCCACAGGAGCTCCCGGCGAAGCCGGACCAGCCGGGGCCCCGGGAGCCCGAGTGGCTTCTGGGGAAGCGGGACCACCCGGAGCTACAGGAGCTCCCGGTGAAGCCGGAGCCGCGGGAGCGACAGGAGCTACCggg                                                                                                                      <  1:1393939/140‑1 (MQ=18)

                       ggtaGCGAGAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCCCAGGGGCTACTGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCCGCCGGCGCGCCCGGAGCTACAgggg                                                                                                                     >  2:1246061/4‑140 (MQ=17)

                       gggAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGCGCCACGGGAGCCACAGGCGCTACGGGGCAAGCCGCACCAGCCGGAGCGACAGGAGCTACCGGCGAAACCGGACCAGCCCGAGCGAGAGGAGCAACCggtg                                                                                                                     >  2:2491129/1‑138 (MQ=14)

                               cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                             <  1:252135/140‑1 (MQ=255)

                                aGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCGACTGGCGAAGGCGGACCAGCCGGAGCGCCCGGAGGGACGGGAGCAGCCGTACCCGCAGGAACGGAAGGTGGTGCGGGGGAAGACGGag                                                                                                            >  2:1999015/1‑139 (MQ=9)

                                     cTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAAcc                                                                                                       <  1:1756426/140‑1 (MQ=255)

                                       aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                     >  1:4823734/1‑140 (MQ=255)

                                       aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGAGAAGCCGGACCACCCGGAGCTACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGAAGCCGGACCAACCgg                                                                                                     >  1:3908815/1‑140 (MQ=18)

                                         cGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCACCCGGAGCGCCCGGAGCAACCGGGGCCCCGGGACGAGCCGGAg                                                                                                   >  2:4798067/1‑140 (MQ=14)

                                             gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                               <  2:6878238/140‑1 (MQ=255)

                                                gCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGATGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAgg                                                                                            <  1:1473228/140‑1 (MQ=17)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAgg                                                                                            >  1:7535584/1‑140 (MQ=255)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGc                                                                                                                 >  2:2342749/1‑119 (MQ=255)

                                                gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                                                                       >  1:5645195/1‑97 (MQ=255)

                                                  cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                          <  2:5395111/140‑1 (MQ=255)

                                                  cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                          <  2:6381808/140‑1 (MQ=255)

                                                     cccAGCCGGGGCCTCCGGTGCCACAGGGGCTTCTGGGGAAGCCGGAGCAGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                       <  1:1887722/139‑1 (MQ=17)

                                                     aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACCGGGGCCCCCGGACCAACAGCAGCGGCAGGAGcga                                                                                       >  1:2881359/1‑138 (MQ=17)

                                                          ccGGCGCCCCCGGAGCCACAGCCGCTACGGGGGAAGCCGGAGCTGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                  <  1:1174681/140‑1 (MQ=11)

                                                              ggCCCCGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:6009653/140‑1 (MQ=255)

                                                              ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                              <  1:6370668/140‑1 (MQ=255)

                                                               gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCAcgg                                                                                    <  2:654154/133‑3 (MQ=255)

                                                               gCCACGCGAGCCACAGGGGAGACCGGGGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGTCAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGc                                                                             <  1:3878651/140‑1 (MQ=14)

                                                                   CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGcgacggg                                                                                                                                <  2:499779‑M1/85‑4 (MQ=255)

                                                                   CGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGcgacggg                                                                                                                                >  1:499779‑M1/1‑82 (MQ=255)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGCCCGGAGCTACCGGGGACTCCGGAc                                                                        >  1:1082291/1‑140 (MQ=14)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                   <  2:2852741/81‑1 (MQ=18)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                   <  2:6938836/81‑1 (MQ=18)

                                                                    gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                                   >  1:6938836/1‑81 (MQ=18)

                                                                           cgcgccgCTCCTCGGGACGCCGGACCCCCCGGCGCCCCAGGCGCTTTCGGGGCAGCCGCACCAGCCGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                 <  2:6693866/134‑1 (MQ=2)

                                                                           acaGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                 <  1:993328/140‑1 (MQ=255)

                                                                           acaGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                 <  1:662898/140‑1 (MQ=255)

                                                                             aGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGCAGCTCCCGGGGACGCCGGACCAGCCGGAg                                                               >  1:6319291/1‑140 (MQ=255)

                                                                              ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGCGCTACCGGGGACGCCGGACCACCCGGAGCGACCGGAGCTACCGGGGACCCCGGGCCGGCCGGAGc                                                              >  2:2644621/1‑140 (MQ=14)

                                                                               gggCGACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                                                             <  1:371191/140‑1 (MQ=255)

                                                                                 gCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                           <  2:3884587/140‑1 (MQ=255)

                                                                                 gCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCAACCGGGGCCCCCGGACCACCCGGACCGAc                                                           >  2:3379128/1‑140 (MQ=11)

                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAAGCCGGCCCAGCCGGCGCGAcg                                                          >  2:5918183/1‑139 (MQ=255)

                                                                                  cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACGGGGGCCCCCGGACCACCAGGAGCGACAGGAGCAACCGAGGAACCCGCGCCACCCGGACCGACa                                                          >  2:4389319/1‑140 (MQ=11)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCGACCGGGGAATCCGGACCAACCGGAGCGCCAGGAGCGACAGGCGCAGCCCGACAAACCGGATCGACAgg                                                        >  2:1125660/1‑140 (MQ=11)

                                                                                    aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                        >  2:618807/1‑140 (MQ=255)

                                                                                      tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATGCGGAGCGGCCGGAGCGACCGGCGCGTCCGGACAAGCCGGACCGCCGGGAg                                                      >  2:6733483/1‑140 (MQ=11)

                                                                                         ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGcga                                                   >  2:1538868/1‑138 (MQ=255)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGACGCCGGTCCAGCCGGCGCGTCCGGAGCGACCGGGGACGCCGGCGCAGCCGGAGCGCCCGGAGCTAcc                                                 >  1:4677822/1‑140 (MQ=11)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                  >  1:3569760/1‑75 (MQ=34)

                                                                                           aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                 >  2:6899574/1‑140 (MQ=255)

                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGTGCGACAGGAGCTCCCGGGGCGGCCGGCCCAGCCGGAGCGACAGGAGCTACCgg                                               >  1:6578977/1‑140 (MQ=14)

                                                                                             gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCCCCCCCGGAGCGACAGGAGCGACAGGGGAAGCCGGGCCAGCCGGCGCGACAGGAGCTACCgg                                               >  2:524043/1‑140 (MQ=14)

                                                                                               cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGACGCCGGACCCGCCGGAGCGCCCGGAGCTACCgggg                                             >  2:4855869/1‑140 (MQ=14)

                                                                                                  cccAGCCGGCGCGACCGGCGCTACCGGGCCAGCCGGAGCAGCCGGAGCGACAGGAGCACCCGGCGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                          <  1:2158622/139‑1 (MQ=14)

                                                                                                  aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                          >  1:2356722/1‑140 (MQ=255)

                                                                                                    cccccGGAGCGACAGGCGCTACCGGGGAAGCCGCACCACCCGGAGCGACAGCAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                        <  2:1748550/137‑1 (MQ=14)

                                                                                                           aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCGCCCGGGGAAGCCGGACCaa                                 >  1:3030919/1‑140 (MQ=255)

                                                                                                            gCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                <  2:7655476/140‑1 (MQ=255)

                                                                                                                 cggcgcgaCCGGGGAACCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                           <  1:2575069/133‑1 (MQ=255)

                                                                                                                 aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGGGAAGCCGGACCATCCGGac                           >  2:5883439/1‑139 (MQ=255)

                                                                                                                 aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAg                           >  2:891238/1‑140 (MQ=255)

                                                                                                                 aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGAGCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTCCCGCGGACGCCGGACCAACCGGAg                           >  1:4722039/1‑140 (MQ=14)

                                                                                                                   gAGCTACCGGCGATCCCGGCCCAGCCGGAGCCCCAGGCGCTACCGGCGCCCCGGGAGCAACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCCTCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                         <  1:544774/140‑1 (MQ=14)

                                                                                                                   gAGCTACCGGCGAAGCCGGACCCGCCCGAGGGCCAGGAGCTTGCGGGGCTGCGCGACCATACGGCGATACCGGACCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                         <  1:6732796/140‑1 (MQ=9)

                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCTACCgg                                                                                   >  1:400471/1‑80 (MQ=21)

                                                                                                                     gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                       >  2:1863899/1‑140 (MQ=40)

                                                                                                                        aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACATGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                    >  1:2462111/1‑140 (MQ=255)

                                                                                                                         ccGGCGCAGCAGGACCACCCGGAGCCCCAGGAGCACCCGGTGTCGCGGGAGGAACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGa                   <  1:556014/140‑1 (MQ=14)

                                                                                                                          cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                  >  1:539369/1‑140 (MQ=40)

                                                                                                                            gggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCCGGACCAACAGGACCGACGGGAGcc                >  1:2273415/1‑140 (MQ=255)

                                                                                                                              gaccccGGACCACCCGGAGCCCCCGGAGCTCCCGGAGCAGCCGGACCTACCGGTGCTCCAGCATCATCCGGAGAAGCAGGAGCTTCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  2:3769545/136‑1 (MQ=9)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  1:782184/140‑1 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGCGGACGCCGGACCACCCGGAGCGACGGGAGcccc              >  1:4196533/1‑138 (MQ=11)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGcccc              >  1:5348440/1‑138 (MQ=255)

                                                                                                                              gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  2:7159241/140‑1 (MQ=255)

                                                                                                                              gAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac              <  1:339039/140‑1 (MQ=255)

                                                                                                                               aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGcc                                                                                        >  1:3296955/1‑64 (MQ=18)

                                                                                                                                 gctgtagcAGCCCGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:7472746/133‑1 (MQ=14)

                                                                                                                                 gCCGGACCCGCCGGAGCGACCGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  2:2128754/140‑1 (MQ=255)

                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                              <  2:5732705/57‑1 (MQ=31)

                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                              >  1:5732705/1‑57 (MQ=31)

                                                                                                                                 gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGAAGCAGGACCAGCCGGAGCGACAGTAGCGACCGGGGGCGCCGGACCACACGGAGCCACGGGAGCGACAgc           >  2:3640394/1‑139 (MQ=14)

                                                                                                                                 ccgggACCCCCCGCAGCGACAGCAGCTACCGGCGAAGCCGGACCCCCCGGAGAGACAGGAGCTACCGGCGAAGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgg           <  1:2046809/137‑1 (MQ=14)

                                                                                                                                   cGGACCCGCCGGAGCGACAGGCGCTTCCGGCGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg         <  1:3128653/140‑1 (MQ=14)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACGGGGGCAGCCGGACCAGCCGCAGCGCCCGGAGCGGCCGGGGGCGCGGGAGCAACCGGAGCGGCGGCAGCGGCAggtg         >  2:5613820/1‑138 (MQ=14)

                                                                                                                                   cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg         <  2:2356722/140‑1 (MQ=255)

                                                                                                                                      accaccCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTa      <  1:26542/140‑1 (MQ=255)

                                                                                                                                      aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                             >  1:866566/1‑101 (MQ=35)

GAAGCTGGACCAGCTGGAGCGACGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGG  >  NZ_CP034943/607500‑607777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`