Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 607,665 G→C 5.2% A170P (GCC→CCC)  EO946_RS03185 → collagen‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943607,6650GC 5.2% 696.1 / 5.1 293A170P (GCC→CCC) EO946_RS03185collagen‑like protein
Reads supporting (aligned to +/- strand):  ref base G (157/109);  new base C (10/10);  total (170/123)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

AGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGG  >  NZ_CP034943/607526‑607804
                                                                                                                                           |                                                                                                                                           
  ccaccggAGCGCCCGGGGAAGCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGCATCTGGGGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                                                                                           <  1:4907139/135‑1 (MQ=17)
     cAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGa                                                                                                                                        <  2:3147866/140‑1 (MQ=255)
           cgcccGGGGAACCCGGACCAGCCGGGGCCCCGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                  <  1:730889/137‑1 (MQ=255)
           cTACCGGGGCAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGTCGGACCAAcc                                                                                                                                  <  1:1756426/140‑1 (MQ=255)
           cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                  >  1:6905225/1‑140 (MQ=255)
           cTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                                                                  <  1:2500553/140‑1 (MQ=255)
             aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGATCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCAACCGGGGAAGCGGGAACaggcgg                                                                                                                                >  1:296352/1‑135 (MQ=17)
             aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCACCCGGGGACCCCGGACCacacgg                                                                                                                                >  2:6226883/1‑135 (MQ=17)
             aCCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCgg                                                                                                                                >  1:4823734/1‑140 (MQ=255)
                   gAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACCGGAGCAACCGGGGAAGCCGGTGCCACCGGAGCGAc                                                                                                                          >  2:5550082/1‑140 (MQ=17)
                   cccgCCGGCCCAGCCGGGGCCACGGGAGCCACCGGGGCGACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCCGCCGGAGCGACCGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                                                          <  1:5370718/137‑1 (MQ=17)
                      gCCGGACCCGCCGGGGCCACGGGAGCCACAGGGGCTACGGGCGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGATGCCGGACCTGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCACCGGAGCGACAgg                                                                                                                       <  1:1473228/140‑1 (MQ=17)
                      gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACAgg                                                                                                                       >  1:7535584/1‑140 (MQ=255)
                      gCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCACCCGGAGCGACCCCAGCTACCGGCGAAGCGGGACCATCCGGAGCGACAGGAGCTAGCGGGGAAGCCGGACCAACCGGCGCGACCgg                                                                                                                       >  2:6124584/1‑140 (MQ=17)
                      gCCGGACCACCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCAGGAGCGACAGGAGATACCGGGGCAGCAGCACCAAGACGAGCCACAgt                                                                                                                       >  1:994662/1‑139 (MQ=17)
                      gCCGCACCAGCCGGGGCCACGCGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                       <  1:2400681/140‑1 (MQ=255)
                        cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCCACCGGAGCGACAGGAg                                                                                                                     >  2:5883712/1‑140 (MQ=255)
                        cGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                     <  2:5395111/140‑1 (MQ=255)
                           cccAGCCGGGGCCTCCGGTGCCACAGGGGCTTCTGGGGAAGCCGGAGCAGCCGGAGCGACCGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTa                                                                                                                  <  1:1887722/139‑1 (MQ=17)
                           aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGCGAAGCCGGACCACCCGCAGCGGCGGGAGccg                                                                                                                  >  2:172997/1‑138 (MQ=17)
                           aCCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGCGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGCGAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCGGCATGACCACCCGGACCGACCGCAGcga                                                                                                                  >  2:6921998/1‑138 (MQ=17)
                                ccGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCggg                                                                                                             >  1:2266750/1‑140 (MQ=255)
                                acGGCGCCACCGGAGCCCCAGGCGCTCCAGGTGACCCCGGACCCCCCGGAGCCACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:851428/139‑1 (MQ=14)
                                    ggCCCCGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:6009653/140‑1 (MQ=255)
                                    ggCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                         <  1:6370668/140‑1 (MQ=255)
                                     gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCAcgg                                                                                                               <  2:654154/133‑3 (MQ=255)
                                     gCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCAcgg                                                                                                               >  1:654154/1‑131 (MQ=255)
                                          gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCAACCGGGGCAGCCGGag                                                                                                   >  1:6679077/1‑139 (MQ=14)
                                          gggAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGCCCGGAGCTACCGGGGACTCCGGAc                                                                                                   >  1:1082291/1‑140 (MQ=14)
                                               ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAATCAGGACCAACCGGAGCGACAGGAGCTACCGGGGATGCCGGACCAGcc                                                                                              >  1:3884587/1‑140 (MQ=255)
                                                 ccaGGGGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGATACCGGGGAAGCCGGACCAGCCgg                                                                                            <  1:5615020/139‑1 (MQ=255)
                                                     gggCGACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCg                                                                                        <  1:371191/140‑1 (MQ=255)
                                                      ggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGa                                                                                       >  2:6682106/1‑140 (MQ=40)
                                                       gCTCCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGAc                                                                                      <  2:3884587/140‑1 (MQ=255)
                                                        cTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACCGGAGCTACGGGGGCCCCCGGACCACCAGGAGCGACAGGAGCAACCGAGGAACCCGCGCCACCCGGACCGACa                                                                                     >  2:4389319/1‑140 (MQ=11)
                                                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                   >  2:6835788/1‑140 (MQ=255)
                                                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                   >  1:1382379/1‑140 (MQ=255)
                                                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAgg                                                                                   >  2:1316649/1‑140 (MQ=255)
                                                          aCTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCCACCGGGGACGCCGGCCCAGCCGGAGCTAGAGTAGATACCGGTGAAGCAGGACACGCCGGACCgggagg                                                                                   >  2:2058885/1‑135 (MQ=11)
                                                            tGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                                                     <  1:993221/104‑1 (MQ=38)
                                                               ggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACCGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGcga                                                                              >  2:1538868/1‑138 (MQ=255)
                                                                gAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAGCCGGAGCGGGAGGAGCCCCCGGCGACGCCGGACCAACCGGAGCTCCATTAGCAAACGGCGAAGCCGGACCACCACGCGCGAGAGGAGATAc                                                                             >  1:2764024/1‑140 (MQ=2)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCATCCGGAGCGACAGGAGCTACCGGGGACGCCGGTCCAGCCGGCGCGTCCGGAGCGACCGGGGACGCCGGCGCAGCCGGAGCGCCCGGAGCTAcc                                                                            >  1:4677822/1‑140 (MQ=11)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTAcc                                                                            >  1:2558169/1‑140 (MQ=255)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAAGCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAGCCGGAGCTAGAGGAGcagcc                                                                            >  1:2605581/1‑136 (MQ=14)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                             <  2:3569760/75‑1 (MQ=34)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                                                                                             >  1:3569760/1‑75 (MQ=34)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGTCAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGACGGTAGCGAcc                                                                            >  1:214080/1‑140 (MQ=11)
                                                                 aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTCCCGGGGAAGCCGGACAAGCCGGAGCGACAGGAGCGAcc                                                                            >  2:4850454/1‑140 (MQ=255)
                                                                   gCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGCAGCCGGCCCCCCCGGAGCGACAGGAGCGACAGGGGAAGCCGGGCCAGCCGGCGCGACAGGAGCTACCgg                                                                          >  2:524043/1‑140 (MQ=14)
                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCTGCCGGAGCGACAGGAGCTACCGCGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGCCGGGAGCTCCCgggg                                                                        >  1:6591910/1‑140 (MQ=14)
                                                                     cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACCCCGGACCACCCGGAGCGACAGGAGCTCGGGGGGTCCCCGGCCCAGCCGGAGCGTCGGGAGCTAgggggg                                                                        >  2:2671817/1‑140 (MQ=11)
                                                                        aCCCGCCGGAGCGCGCGGAGCTACGGGCGCCGCCGGACCTCCGGCAGCTACAGGAGATACCGGGGCAGCGGGACCTTCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAg                                                                     <  2:6895396/140‑1 (MQ=2)
                                                                          cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                   <  2:2266750/140‑1 (MQ=255)
                                                                          cAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGcc                                                                   <  2:1858539/140‑1 (MQ=255)
                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:1978866/95‑1 (MQ=37)
                                                                             ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCggg                                                                                                             >  2:1978866/1‑95 (MQ=37)
                                                                                 aGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACGCCGGACCac                                                            >  2:7535366/1‑139 (MQ=255)
                                                                                      cAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGCCCAGGCGGAGCGCCAGGAGCTACCGGGGAAGCCGGGCCAGCCGGAGCGCCAGGCGCTACCGGGGAGGCCGGAGCATCCGGa                                                       >  2:1394760/1‑140 (MQ=11)
                                                                                       aGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                      >  1:7655476/1‑140 (MQ=255)
                                                                                         gAGCTACCGGCGATCCCGGCCCAGCCGGAGCCCCAGGCGCTACCGGCGCCCCGGGAGCAACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCCTCCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                    <  1:544774/140‑1 (MQ=14)
                                                                                         gAGCTACCGGCGAAGCCGGACCCGCCCGAGGGCCAGGAGCTTGCGGGGCTGCGCGACCATACGGCGATACCGGACCGACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                    <  1:6732796/140‑1 (MQ=9)
                                                                                           gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGGCGGAGCGACAGGAGCTACCGGGCAACCCGGACCAACCGGAGCGAc                                                  >  2:3333605/1‑140 (MQ=255)
                                                                                                cGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAg                                             >  1:539369/1‑140 (MQ=40)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACCGGAGCCACAGGGGCAGCAGGACCAGCCGGAGCGACCGGAGCTACCGGCGAAGCCGGACCAGCCGGAGCTACCGGAGCcac                                         >  2:5737005/1‑140 (MQ=11)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                                                       >  1:4001567/1‑62 (MQ=35)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                         <  1:1158628/140‑1 (MQ=255)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACAAGACGGAGCGGCAGGAGCTACCGGTGAAGCCGGACCAACCGGAGCGACGGGAGCaac                                         >  2:369071/1‑140 (MQ=14)
                                                                                                    gAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCAACCGGGGACGCAGGACCAGCCGGAGCGACAGGAGCAACCGGCGACGCCGGACCACCCGGAGCGCCGGGAGcacc                                         >  2:6430479/1‑137 (MQ=11)
                                                                                                    gAACCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcac                                         <  1:339039/140‑1 (MQ=255)
                                                                                                     caGCCGCACCCTCGGGAGCGACAGGCGCTCCCGGGTAACCCGGACCGCCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCcaca                                        <  1:403968/139‑1 (MQ=14)
                                                                                                     aaGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGcc                                                                                                                   >  1:3296955/1‑64 (MQ=18)
                                                                                                      cgCCGGACCAGGCGGACCCACAGGTGCTACGGGTGAAGCCGGACCCAGCGGTCCCACAGGAGCTACCGGCGAAGCGGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAg                                       <  2:461514/139‑1 (MQ=14)
                                                                                                       gCCGGACCACCCGGCCCCCCAGGAGCCACCGGCGCACCCGGAGGAACCGGAGCGACAGGAGCTACCGGGCCACACGGAGCATCGGGAGCCACATGAGCTACCGTGGAAGCCGGACCACCCGGAGCGAATGGAGCCACAgg                                      <  2:7146358/140‑1 (MQ=2)
                                                                                                       cccgccccACCCGGACCGCCACGCGCCCCCGGTGACGCCGGACCAACCGCACCCACAGTAGCTACCGGGGAAGCGGGACCCGCGGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAACCGGAGCGACGGGAGCCACAgg                                      <  2:6714508/134‑1 (MQ=9)
                                                                                                         cGGACCCGCCGGAGCGACAGGCGCTTCCGGCGAAGCCGGACCAACCGGAGCGACAGGCGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                    <  1:3128653/140‑1 (MQ=14)
                                                                                                         cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                    <  2:2356722/140‑1 (MQ=255)
                                                                                                         cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAgggg                                    >  1:6286163/1‑140 (MQ=255)
                                                                                                            aCCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCgggg                                                                        <  2:866566/101‑1 (MQ=35)
                                                                                                                gCCGGAGCGACAGGAGCTACCGGGGAACCCGGACCAACCGGAGCGACAGGAGCTACCGGCGCAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCCACGGgcgcgccggg                             >  2:5074445/1‑134 (MQ=14)
                                                                                                                 ccGGAGCGACAGGCGCTCCCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                            <  1:1640955/140‑1 (MQ=255)
                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTAc                                                                                                                 >  1:6106285/1‑55 (MQ=35)
                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTggg                            >  2:816836/1‑140 (MQ=255)
                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                            <  1:4234476/140‑1 (MQ=255)
                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTggg                            <  1:3854040/140‑1 (MQ=255)
                                                                                                                 ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAGGCCGGACAAAGCGGAGCGACGGGATCCCCAGGCGCTACAggg                            >  2:3000016/1‑140 (MQ=14)
                                                                                                                           aGGCGCTCCCGGGGCAGCCGGACCAACCGGAGCGACAGGACCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCCCAGGGGCTACTGGGGACGTCGGAc                  <  1:2354572/140‑1 (MQ=18)
                                                                                                                           aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACTGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                  >  1:4864661/1‑140 (MQ=255)
                                                                                                                                  aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCTACCGGAGCGCCGGGAGCCACAGGGGGTCATGGGGACGACGGACCAGCCgg           >  1:5249571/1‑140 (MQ=17)
                                                                                                                                  aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg           >  1:5102687/1‑140 (MQ=255)
                                                                                                                                  aCCGGGGAACCCGGACCCACCGGAGCGACAGGAGCTACGGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCgg           <  2:6319291/140‑1 (MQ=255)
                                                                                                                                    cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAGCCGGAGCGCCCGGAGCTACCGGGGCCGCCGGAGCAACCGGAGCGACGGGAGCCAGAGGGGCTACGGGGGACGTCGGACCAGCCGGAg         >  2:4787429/1‑140 (MQ=18)
                                                                                                                                    cGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGGCGGACCAGCCGGAg         >  2:4882269/1‑140 (MQ=255)
                                                                                                                                       cgacgCCGCACCCACCGGAGCGCCAGGACCCCCCGGCGACGCCGGACCAGGCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGa      <  1:3938729/136‑1 (MQ=18)
                                                                                                                                        gcaGCAGGACCCACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc     <  2:5990153/138‑1 (MQ=255)
                                                                                                                                        gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGGCGCCCCGGCGCGACGGGAGCCACTGGGGCGACGGGGGAGGGCGGGCCGGCCGGAGCgcc     >  2:3785503/1‑138 (MQ=14)
                                                                                                                                         aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                               <  1:565763/97‑1 (MQ=37)
                                                                                                                                         aaGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACggg                                               >  2:565763/1‑97 (MQ=37)
                                                                                                                                           gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCCACAGGCGCAACAGGCCCAGTCGCACCAGCCGGAGCGCCAgc  >  2:4068156/1‑139 (MQ=14)
                                                                                                                                           gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAgg  >  2:7215212/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGGGCCACGGGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGG  >  NZ_CP034943/607526‑607804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: