BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	607,783	T→C	5.1%	V209A (GTC→GCC)	EO946_RS03185 →	collagen‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	607,783	0	T	C	5.1%	2345.2 / 46.3	826	V209A (GTC→GCC)	EO946_RS03185	collagen‑like protein
Reads supporting (aligned to +/- strand): ref base T (399/339); new base C (14/28); total (438/388)
Fisher's exact test for biased strand distribution p-value = 1.06e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 829 total aligned reads displayed.

CAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGC  >  NZ_CP034943/607648‑607921

 aGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGAc                                                                                                                                       <  1:3099079/140‑1 (MQ=255)

        aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGAGCAGCCGGAGCCACAGGAGCGACCGGGGCCGCCGGAGCAACCGGAGCGCCGGGAGCCAGAGTGGCTACGGGGGGGGGCGGACGCGCCgg                                                                                                                                >  2:4452421/1‑140 (MQ=14)

        aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCCGCCGGAGCGACAGGAGCAACCGGGGCAGGCGGACAAACCGGTGCGACGGGAGCCACAGGGGCTACTGGGGAGGTCGGGccggccgg                                                                                                                                >  2:6012776/1‑140 (MQ=17)

        aCCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCGACCGGGGAACCCGGACCAGCCGGAGCGACGGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACCGGGCCTCCTGGGGCCGGCGCACCAGCggg                                                                                                                                >  1:5853795/1‑140 (MQ=14)

              gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACCGGAGCTACCGGGGAACCCGCGGCCACCGGCGCGACAGGAGCCACCGGGGCTACAGGGGACGGCGGCCCAGCCGGAGCGAc                                                                                                                          >  2:7138111/1‑140 (MQ=17)

              gAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGAAGCCGGGCGCCCCGGCGCGACGGGAGCCACTGGGGCGACGGGGGAGGGCGGGCCGGCCGGAGCgcc                                                                                                                          >  2:3785503/1‑138 (MQ=14)

                 gCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACCGGAGCCACAGGCGCAACAGGCCCAGTCGCACCAGCCGGAGCGCCAgc                                                                                                                       >  2:4068156/1‑139 (MQ=14)

                  ccGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGa                                                                                                                      <  1:369071/140‑1 (MQ=255)

                     gaccgacgggcgccacaggggcgaccggggaaggCGGACCAGCCGGAGCCACAGGAGCTACCGGGGAAGCCGGTCCATCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCt                                                                                                                   <  1:542897‑M2/106‑1 (MQ=255)

                      cccccccGGAGCGACAGGAGCAACCGGTGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGAAGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  <  1:1472259/135‑1 (MQ=18)

                      aCCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTa                                                                                                                  >  2:7507129/1‑140 (MQ=255)

                           ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCggg                                                                                                             <  1:6835788/140‑1 (MQ=255)

                             ggagccacaggggctactggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGa                                                                                                           >  1:6008496‑M2/27‑140 (MQ=255)

                              gagccacaggggctactggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGaa                                                                                                          <  1:2952953‑M2/115‑1 (MQ=255)

                                         gctactggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAc                                                                                               >  2:5395666‑M2/15‑140 (MQ=255)

                                         gCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGCGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCacc                                                                                               >  2:3547149/1‑138 (MQ=255)

                                         gCTACCGGGGAACCCGGCCCAGCCGGAGCGTCAGGCGCGACCGGGGCCGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCacc                                                                                               <  1:1041894/140‑3 (MQ=18)

                                          ctactggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAAcc                                                                                              >  2:829417‑M2/14‑140 (MQ=255)

                                            actggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTCCCGGGGACCCCGGACCAACcgc                                                                                            >  2:6296024‑M2/12‑139 (MQ=255)

                                            actggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  2:4619452‑M2/12‑140 (MQ=255)

                                            actggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  1:7412220‑M2/12‑140 (MQ=255)

                                            actggggacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACC‑GGGAAGCCGGACCAACCGGa                                                                                           >  1:3976827‑M2/12‑140 (MQ=255)

                                            aCCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCgg                                                                                            >  2:2956923/1‑140 (MQ=255)

                                              cgtggACGCCGGACCAGCCGTCGCCACCGGAGCTACAGGGGAATCCGGTCAATCCGGAGCCGCGTGAGCCACAGGGGCTACTGGGGAAGCCGGACCAGCGGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAg                                                                                          <  2:3254522/137‑1 (MQ=11)

                                                gggacgcCGCACCACCCGGAGCCACAGCAGCTACCGGGGAGGCCGGACCAACCGGAGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCACCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCg                                                                                        <  1:3920969‑M2/133‑1 (MQ=255)

                                                  gacgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGAc                                                                                      >  1:7544023‑M2/6‑140 (MQ=255)

                                                   acgtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCGACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACa                                                                                     >  2:5059464‑M2/5‑140 (MQ=255)

                                                     gtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  2:1391399‑M2/3‑140 (MQ=255)

                                                     gtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  2:524639‑M2/3‑140 (MQ=255)

                                                     gtCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAgg                                                                                   >  2:5790455‑M2/3‑140 (MQ=255)

                                                       cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggag                                                                                 <  2:2288772‑M2/140‑8 (MQ=255)

                                                       cGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAg                                                                                 >  1:7041369/1‑140 (MQ=255)

                                                        ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGc                                                                                >  2:3153663/1‑140 (MQ=255)

                                                        ggACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGAAAGTCGGACCAGCCTTAGCGACAGCAGCTACAGGGGAAGACTGACCAACCGGAGCGAGAGGaac                                                                                >  2:2591206/1‑138 (MQ=14)

                                                           ccAGCCGGCGCGCCCGGAGCCACCGGGGAAGCCGGCCCACCCGGAGCGCCGGGAGCCACAGGGGCTACGGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccac                                                                             >  1:1173270‑M2/1‑129 (MQ=255)

                                                           ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccac                                                                             >  1:4882269‑M2/1‑129 (MQ=255)

                                                           ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccac                                                                             >  1:7245877‑M2/1‑129 (MQ=255)

                                                           ccAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccac                                                                             >  2:3965396‑M2/1‑129 (MQ=255)

                                                            cacccGGAGCCACCGGAGCGCCCGGGGAACCCGGACCAGCCGGCGCGACGGGTGACACAGGGGCTAAGGGGGACGTCGGCCCAGCCGGAGGGACAGGAGCTCCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCAca                                                                            <  1:909707/137‑2 (MQ=17)

                                                             aGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACgg                                                                           <  1:6424526/140‑1 (MQ=255)

                                                              gCGGGAGCGCCTGGAGCTCCCGGGGCAGCCGGACCACCCGGCGCGCCGGGAGCCACTGGGGCTCCTGGGGACCTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGacgggagccacagg                                                                          >  1:4416876‑M2/1‑126 (MQ=255)

                                                              gCCGGAGCGACAGGAGCGACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                                                          >  1:6313963‑M2/1‑126 (MQ=255)

                                                              gCCCGAGCGACAGGAGCTCCCGGGGCAGCCGGACCACCCGGAGCCACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                                                          >  1:6234451‑M2/1‑126 (MQ=255)

                                                               ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                         >  1:4322604/1‑140 (MQ=255)

                                                               ccGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGa                                                                         >  1:180704/1‑140 (MQ=255)

                                                                 ggAGCCACCGGAGCTACCGGCGATGCGGGCCCACCCGGAGCCTAGGGCGCCACAGGGGCTACTGGGGACGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGc                                                                       <  1:2671817/140‑1 (MQ=14)

                                                                  gAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGcc                                                                      <  1:2288772/140‑1 (MQ=255)

                                                                   ggccaCAGGCGCCCCCGGGGAACCCGGACCAACCGGAGCGCCGGGCGCCACCGGGGCAACGGGGGACGCCGGACCCCCCGGAGCGACAGGAGCTACCGGGGCAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGcta                                                                     <  2:2809908/136‑3 (MQ=9)

                                                                        cAGGCGCTACCGGGGAAGCCGGCCCAACCGGCGCGACGGGAGCCACCGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacaggggctactggg                                                                >  1:2033886‑M2/1‑116 (MQ=255)

                                                                        cAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccaccggggcgactggc                                                                <  2:6985919‑M2/140‑25 (MQ=255)

                                                                         aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGACGCCGGACCAACCGGAGCGacgggagccaccggggcgactgggc                                                               <  2:4755029‑M2/140‑26 (MQ=255)

                                                                         aGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGCGCGACAGGAGCCACGGGAGCcacaggca                                                               >  2:5539107/1‑140 (MQ=255)

                                                                             gCGACCGCGGCCGCCGGCCCACCCGGACCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGacgggagccacaggggctactggggacgt                                                           >  2:6433921‑M2/1‑111 (MQ=255)

                                                                               tACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGag                                                         >  1:1370480/1‑140 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                        >  2:6133082/1‑140 (MQ=255)

                                                                                aCCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagc                                                        >  2:204754/1‑140 (MQ=255)

                                                                                 ccGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGAc                                                                                                                          <  1:1512201/73‑1 (MQ=255)

                                                                                      gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacaggggctactggggacgtcggaccagc                                                  <  1:4994577‑M2/140‑39 (MQ=255)

                                                                                      gAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCAACAGGCACAGGAGCAGCTgg                                                  >  2:4289119/1‑140 (MQ=255)

                                                                                         gCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagcccccggggctactggggacgtcggaccagccgg                                               <  1:1949456‑M2/140‑42 (MQ=255)

                                                                                           cGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGAcc                                                                                                  <  2:3628201/87‑1 (MQ=255)

                                                                                              aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                          >  2:2684019/1‑140 (MQ=255)

                                                                                              aCCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCg                                          >  1:4208247/1‑140 (MQ=255)

                                                                                                 aaCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAAt                                       <  1:2553765/140‑1 (MQ=255)

                                                                                                  aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacaggggctactggggacgtcggaccagccggagcgacagg                                      <  1:770974‑M2/140‑51 (MQ=255)

                                                                                                  aCCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCAGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  1:6562836/140‑1 (MQ=255)

                                                                                                  aCCGGAGCGACCGGCGCCACAGGGGCTACTGGGGACGTCCGACCAGCCGTAGCGACAGGAGCGACAGGGGAAGCCGGACCAAACGGAGCTCAAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATa                                      <  1:2791015/140‑1 (MQ=25)

                                                                                                   ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                                                          >  1:2163113‑M2/1‑89 (MQ=255)

                                                                                                   ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccacagg                                                                          <  2:2158922‑M2/103‑15 (MQ=255)

                                                                                                   ccGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATAc                                     <  2:781177/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:6131687/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:5276521/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  2:1512903/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:5724317/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:4655489/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  1:3768339/140‑1 (MQ=255)

                                                                                                       aGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 <  2:6536852/140‑1 (MQ=255)

                                                                                                        gCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGta                                <  1:1619188/140‑1 (MQ=255)

                                                                                                                  ccACAGGGGCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGacgggagccac                                                                             <  2:5659805‑M2/85‑12 (MQ=255)

                                                                                                                      aGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTaa                  >  2:3455131/1‑140 (MQ=255)

                                                                                                                       ggggCTACTGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGt                                 >  2:1713848/1‑124 (MQ=255)

                                                                                                                         ggCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCTAATACGGGTATATATATAACTTAAtcg               >  2:1924937/1‑140 (MQ=255)

                                                                                                                           cTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCt             <  1:5087992/140‑1 (MQ=255)

                                                                                                                             accggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                           <  1:1315854/89‑1 (MQ=255)

                                                                                                                             accggggAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCcacaggcacagg                                                           >  2:1315854/4‑92 (MQ=255)

                                                                                                                             aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                      <  2:6374413/97‑1 (MQ=255)

                                                                                                                             aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGACTCCGGGTCTATATATAACTTAAGCGCTCa           >  2:6607609/1‑140 (MQ=255)

                                                                                                                             aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa           <  1:3330810/140‑1 (MQ=255)

                                                                                                                             aCTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCa           >  2:1109094/1‑140 (MQ=255)

                                                                                                                               tGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCaaa         >  1:4557219/1‑140 (MQ=255)

                                                                                                                                ggggACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAAc        <  1:826605/140‑1 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGagcag                                                      >  1:7156728/1‑91 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     >  2:6723331/1‑140 (MQ=255)

                                                                                                                                   gACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     >  1:5584051/1‑140 (MQ=255)

                                                                                                                                    tggTCGGAACAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCACCCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa    <  1:6702683/138‑1 (MQ=255)

                                                                                                                                    aCGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTa    >  2:4862032/1‑140 (MQ=255)

                                                                                                                                     cGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAg   <  1:3565756/140‑1 (MQ=255)

                                                                                                                                      gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTAt                                             <  2:7221016/97‑1 (MQ=255)

                                                                                                                                      gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     >  1:2391772/1‑137 (MQ=255)

                                                                                                                                      gTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGt     <  2:2391772/137‑1 (MQ=255)

CAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACGGGAGCCACAGGGGCTACTGGGGACGTCGGACCAGCCGGAGCGACAGGAGCTACCGGGGAAGCCGGACCAACCGGAGCGACAGGAGCCACGGGAGCCACAGGCACAGGAGCAGCTGGGTTATCCGAATACGGGTATATATATAACTTAAGCGCTCAAACGGTAGC  >  NZ_CP034943/607648‑607921

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`