Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,052 T→G 14.3% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0520TG14.3% 696.6 / 27.0 337E573D (GAA→GACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (114/158);  new base G (61/3);  total (175/162)
Fisher's exact test for biased strand distribution p-value = 1.05e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 355 total aligned reads displayed.

TGTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAA  >  NZ_CP034943/633913‑634187
                                                                                                                                           |                                                                                                                                       
tGTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGt                                                                                                                                         >  2:5637430/1‑140 (MQ=255)
tGTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGt                                                                                                                                         >  2:3369055/1‑140 (MQ=255)
  tCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGttg                                                                                                                                       >  1:922375/1‑139 (MQ=255)
  tCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATACGACGGAGTCGTTGGCGttg                                                                                                                                       >  2:5592624/1‑139 (MQ=255)
      cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGttctttc                                                                                                                                   <  2:4673826/140‑1 (MQ=255)
      cATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATATCTTCAATCCGACGGAGTCGTTGGCGttctgtg                                                                                                                                   >  2:2971875/1‑137 (MQ=255)
            tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGtttttg                                                                                                                             >  1:4167707/1‑139 (MQ=255)
            tggCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTc                                                                                                                             <  2:2047179/140‑1 (MQ=255)
              gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTATTTTTACa                                                                                                                           >  2:5141093/1‑140 (MQ=255)
              gCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCa                                                                                                                           <  2:5024409/140‑1 (MQ=255)
                  gTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCt                                                                                                                       <  2:264767/140‑1 (MQ=255)
                        tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTGCGTTAtttttgt                                                                                                                 >  1:2204061/1‑138 (MQ=255)
                        tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGGTTTGTTTTTGAATTAtttttat                                                                                                                 >  2:5771635/1‑138 (MQ=255)
                        tttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTCTTTTTCCATTCTTTttct                                                                                                                 >  2:1495798/1‑140 (MQ=255)
                         ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTTCATTATTTttatt                                                                                                                >  1:5593963/1‑140 (MQ=255)
                         ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTGCATTCTTTttatt                                                                                                                >  1:6661805/1‑140 (MQ=255)
                         ttCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTATTTttatt                                                                                                                >  2:5352165/1‑140 (MQ=255)
                            aTGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTttcttctt                                                                                                             <  1:2650348/140‑1 (MQ=255)
                             tGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTACATTCTTTttcttatta                                                                                                            >  2:2537756/1‑139 (MQ=255)
                             tGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTGCATTGTTTttgttattg                                                                                                            >  2:234234/1‑139 (MQ=255)
                                  ttGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATg                                                                                                       <  2:2461260/140‑1 (MQ=255)
                                    gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCAATGGTTTTGTTGTTCATATGaa                                                                                                     >  1:3667244/1‑140 (MQ=255)
                                    gATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGATTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGaa                                                                                                     <  2:1803701/140‑1 (MQ=255)
                                        gATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGtt                                                                                                 <  1:1489717/140‑1 (MQ=255)
                                         aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTGCATTGTTTTTGTTCTTAATATGaaaggtt                                                                                                >  2:3927025/1‑135 (MQ=255)
                                         aTTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGttt                                                                                                <  2:1272937/140‑1 (MQ=255)
                                          tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAGTGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTGGTTTGTTGTTGCAATATTTTTGTTCTTCATATTAACCtttt                                                                                               >  1:5315426/1‑140 (MQ=255)
                                          tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTGTTTTTCCATTCTTTTTCTTATTCATATTCAAGtttt                                                                                               >  2:354097/1‑140 (MQ=255)
                                          tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTGCAATATTTTTGTTATTAATATTAAAGtttt                                                                                               >  1:2505548/1‑140 (MQ=255)
                                          tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTGTTTGTTTTTGCATTCTTTTTGTTCTTCATATTAAAGtttt                                                                                               >  1:1611019/1‑140 (MQ=255)
                                            tCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTCCATTGTTTTTCTTATTGATATGGACGTTTTgt                                                                                             >  2:2490091/1‑139 (MQ=255)
                                              cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  2:6719080/140‑1 (MQ=255)
                                              cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:300635/140‑1 (MQ=255)
                                              cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  2:2994470/140‑1 (MQ=255)
                                              cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:6310024/140‑1 (MQ=255)
                                              cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                           <  1:4251008/140‑1 (MQ=255)
                                              cGGATCACATAGCAAGTCATCGTTATGACTAATGTTTTCTTCAATGGATTGAACACTGGCTTCAATTTCTTCAATCCGACGGACTCGTTGGCGTTCTTTCATTTTCCATTCTATTTCTTCTTCATATGAACGATTTGAGt                                                                                           <  2:3081282/140‑1 (MQ=255)
                                                  tCACATAGCAATTCATCGTTATGACTACTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAgg                                                                                       <  1:6519782/140‑1 (MQ=255)
                                                  tCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAgg                                                                                       <  1:2109310/140‑1 (MQ=255)
                                                      aTAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTGCATTCTTTTTGTTGTTCATATGAACGTTTTGTTTCAGGGttt                                                                                   >  1:5719163/1‑139 (MQ=255)
                                                       gaGCACTTCATAGTTATGACGAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCGTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGCACGTTTTGAGTCAGGCTTaa                                                                                  <  2:6456345/139‑1 (MQ=255)
                                                        aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                 <  1:687226/140‑1 (MQ=255)
                                                        aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                 <  1:770521/140‑1 (MQ=255)
                                                        aGCAATTCATCGTTATGACGAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                 <  1:1022664/140‑1 (MQ=255)
                                                             ttCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTGCATTGTTTTTCTTCTTCATATTTACGTTTTGATTCAGGCTTAAACGAAg                                                                            >  2:347706/1‑140 (MQ=255)
                                                               cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTATTTTTACCTTCTTTTTCTTATTAAAATGAACGGTTTGAGTCAGGCTTAAACGAAgaa                                                                          >  2:7331/1‑138 (MQ=255)
                                                               cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAACGAAGCa                                                                          >  2:5683822/1‑140 (MQ=255)
                                                               cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTGTTTTTCCCTTCTTTTTCTTCTTGATGTGAACGTTTTGAGTCAGGCTTAACCGAAGct                                                                          >  2:6927335/1‑139 (MQ=255)
                                                                aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCTTTTTCCATTCTTTTTCTTCCTCATATGAACGTTTTGAGTCAGGCAGAACCGAAGCac                                                                         >  2:894433/1‑139 (MQ=255)
                                                                aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTACCTTGGTTTTCGTCGTCATATGTACGTTTTTTGTCAGGCTTAACCGcagcag                                                                         >  2:2914529/1‑140 (MQ=255)
                                                                aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTGCTTTCTTTTTACATTGTTTTTCTTCTTCATATTAACGTTTTTAGTCAGGCTTAACCGAAGCAg                                                                         >  2:5721178/1‑140 (MQ=255)
                                                                  cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTCGAATGTTTTTGTTGTTGCTATTTACGTTTTTTGTGATGGTTAAACGAATCAggg                                                                       >  1:4920833/1‑139 (MQ=255)
                                                                  cGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTGTTTGTTTTTGCATTCGTTTTCTTGTTGCTATGTACGGTTTGTGTCAGGCTTAAACGAAGCAGGc                                                                       >  1:1918221/1‑140 (MQ=255)
                                                                   gTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCg                                                                      <  2:6594243/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCATTTGTTTGAACAGTTGCTTCAATTTCTTCAATCCTACGTAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCTTTTTTTCGGa                                                            <  2:6355874/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCACTGGTTTGAACAGTGGCTTCAATTTCTTCACTCCGACGGAGTCGTGGGCGTTCTTTCTTTGTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  1:6090202/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTTTGTGAGGCTTAACCGAAGCAGGCGTTTTTTCggt                                                            >  2:1452569/1‑139 (MQ=255)
                                                                             aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGCGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  1:7469589/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  1:3515084/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  2:409706/140‑1 (MQ=255)
                                                                             aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                            <  2:6178101/140‑1 (MQ=255)
                                                                              tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAAACCGACCGAGTCGTTGGCGTTCATTATTTTTCCAAACTTTTTATACGTCCAAAGACAGGTTTGTGTCAGGGGTTACCGACTCAGCCGtttttggtgat                                                           >  2:4808787/1‑134 (MQ=255)
                                                                                tgttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGATTAACCGAAGCAGGCGTTTTTTCGGATTc                                                         <  1:4104417/138‑1 (MQ=255)
                                                                                  ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGGTTGTTTTTGCATTATTTTTGTTGTTAATATTAACATTTTTAGTGAGTGTTTAACGAAGCCCGCGTTTTTTGGGAttacg                                                       >  1:734451/1‑137 (MQ=255)
                                                                                  ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTCCATTATTTTTGTTGTTGATAGGAACGTTTTTAGTGAGGGGTAACCGCACCAGGCGGTTTTTGGGGttgcg                                                       >  1:3950379/1‑137 (MQ=255)
                                                                                   tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGGTGTTTGTTTTTGCAATGTTTTTGTTTTTAATATGTACGTTTTTAGTGAGGCTTAAACGAATCAGGCGGTTTTTGGGGttgcgg                                                      >  1:6159078/1‑136 (MQ=255)
                                                                                     ttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTCGTTTTACCTTCTTTTTATTCTTAATATGAAAGTTTTTAGTAAGGCTTAAACGACGCAAGCGTTTTTTAGGATTCCTAtt                                                    >  2:3180029/1‑140 (MQ=255)
                                                                                     ttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAAAGTTTTGAGTGAGGCTTAAACGAAGCAGGCGTTTTTTAGGATTCCTCtt                                                    >  2:5529906/1‑140 (MQ=255)
                                                                                        aaTGGTTTGAAGAGTGGCTTCAAGTTCTTCAATCAGACGTAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACAGAAGCAGGCGTTTTTTCGGATTCCTCTTGCt                                                 <  2:7641384/140‑1 (MQ=255)
                                                                                          tGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTCCATTCTTTTTGTTATTCATAAGAACGTTTTGTGTCATGCTTTAACGCATCAGGCGTTTTTTGGGATTTCTATTGGTGa                                               >  2:1045788/1‑140 (MQ=255)
                                                                                           ggTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGAt                                              <  2:3988094/140‑1 (MQ=255)
                                                                                               tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGGTTGTGTTTGCCTTCTGTTTGTTGTTGATATGAAAGGTTTGTGTGAGGCTTAACCGAAACCGCCGTTTTTTTGGAGTCGTCTTGGGGATTGAt                                          >  1:5015011/1‑140 (MQ=255)
                                                                                                gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTGTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                         <  2:2114338/140‑1 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTGTTTATTTTTCCAGTGTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCCCAGCAGGCGTTTTTTAGGATTAGTCTTGGTGATTCATTTTATcc                                   >  2:4240773/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTTGATTGTTTTTGTTGTTGATATTTAAGTTTTTTGTGAGGGTTTAACGAATCAGGGGTTTTTTTGGGTTTCTGTTTGTGTTTGATTTTTTcc                                   >  1:7070046/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTGTTTTTGGATTCTTTTTGTTGTTGATATGAACGTTTTTAGTCAGGCTTAAACGAATCAGGCGTTTTTTGGGGTTACTCTTTCTGATTGAttttatac                                   >  2:2210033/1‑136 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTATTTTTGCATTGTTTTTCTTCTTGATATGTAAGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGCTTGCTTTTCTcc                                   >  2:5886288/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTATTTTTGTTGTTGATATTAAAGTTTTTTGTCAGGCTTAAACGAAACAGGCGGTTTTTGTGATTTCTCATTGTTATTTAttttatac                                   >  1:7174011/1‑136 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTGTTTTTCCCTTTTTTTTGGTCTTAATATGGACGTTTTTTGTAAGGGTTAACCGAAGCAGGGGTTTTTTGGGATTGCTATTTCTGGTTCATTTTGTcc                                   >  2:2772470/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCTTTTTGCATTATTTTTCTTCTTAATATGAAAGTTTTTAGTAAGGCTTAAACCAAGCAGGCGTTTTTTCGGGTTCCTCTTGCTGATTAATTTTATcc                                   >  2:383665/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTGTTTTTCCATTCTTTTTCTTCTTAAAATGAACGTTTTGAGTCAGGCTTAAACGAAGCAGGCGTTTTTTAGGATTACTAGTTCTGTATCATTTTGTcc                                   >  2:4998626/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTGAGGCTTAACCGAAGCAAGCGATTTTTAGAATAACTCATGATGATTAAATTtctaa                                   >  2:7166494/1‑138 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGTTTTTCCATTATTTTTATTCTTAATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTcc                                   >  2:1611907/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTCCATTATTTTTCGTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAACCCGGCGTTTTTTCGGATTCCTCTTGGTGGTTGATTTTGTcc                                   >  2:6090034/1‑140 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTATTTGTTTTTACAGTCTTTTTCTTATTCATATTAACGTTTTTAGTGAGGGTTAAACCAAGCAAGGGTTTTTTAGGGTTACTCTTTGTGATTAAttttatgc                                   >  2:3037884/1‑136 (MQ=255)
                                                                                                      tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCATTCTTTTTATTGTTCATATTAACGTTTTGTGTCAGGCATTACCCAACCACGCGGTTTTTGGGGTTGCGCTTTCTGTTTGAttttgtgc                                   >  1:7564054/1‑136 (MQ=255)
                                                                                                       ggCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTGTTTGGTTTTCCATTGTTTTTCTTCTTCATATGAGCGGTTTGATTCAGGCTTAAACGAAGCAGGCGTTTTTTAGGAGTCCTCTTGGTGTTTCAttttgtgac                                  >  2:4590638/1‑135 (MQ=255)
                                                                                                              aTTTCTTCACTCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTATTTCGTCTTCATATGAACGTTGTGAGTCCGGCTTAACCGAAGAAGGCGTTTTTTCGTATTCCACTTGCTGATTCATTTTCTCCAATTCAAg                           <  1:3573979/140‑1 (MQ=255)
                                                                                                                   ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                      <  2:3674128/140‑1 (MQ=255)
                                                                                                                   gtcAATCCGACGGAGTCGTTGGCGCTCTTTCTTTTTCCATTCTTTTTCTTCCTCGTAGGAACGTTTGGAGTCAGACTTCACTGTAGCTGGCGTTTTATCAGTTTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                      <  2:4325940/139‑1 (MQ=255)
                                                                                                                        tCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTGTTTTTGTTGTTCATATGAACGTTTTGTGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGAGTCCGCTTGCTGGTTCATTTTATGCAATTCAAGCTGCGCTGtt                 >  2:5246115/1‑140 (MQ=255)
                                                                                                                             cGGCGTCGTTGGCGTTCGTTCTTTGTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt            <  2:4180651/140‑1 (MQ=255)
                                                                                                                               gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTACCCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  2:4561245/140‑1 (MQ=255)
                                                                                                                               gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  1:4683183/140‑1 (MQ=255)
                                                                                                                               gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  1:1340247/140‑1 (MQ=255)
                                                                                                                               gAGTCGTTGGCGGTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAGTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct          <  2:6644957/140‑1 (MQ=255)
                                                                                                                                  tCGTTGGCGTTCTTTGTTTTTCCATTCTTTTTCTTCTTCATATGAAAGTTTTTAGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTCCTCTTGGTGATTAATTTTATCCAAATAAAACTGCTCTGTTTTTTTCTACg       >  2:6307324/1‑140 (MQ=255)
                                                                                                                                  tCGTTGGCGTTCGTTCTTTTTCCATTCCTTTTCTTCTTCCTATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg       <  1:590831/140‑1 (MQ=255)
                                                                                                                                       ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa  <  2:3576088/140‑1 (MQ=255)
                                                                                                                                       ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTGTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa  <  1:4590638/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TGTCTGCATGGATGGCTTGTACCTTTTCATGGTCTTGATAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAA  >  NZ_CP034943/633913‑634187

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: