Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 634,089 C→A 5.0% R561L (CGT→CTT)  EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943634,0890CA 5.0% 1063.5 / 3.9 417R561L (CGT→CTT) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (101/274);  new base A (38/2);  total (141/276)
Fisher's exact test for biased strand distribution p-value = 8.14e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

TAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATAT  >  NZ_CP034943/633951‑634218
                                                                                                                                           |                                                                                                                                 
    tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTTCATTGTTTTTGTTATTGATATGTACGtttt                                                                                                                              >  1:3364690/1‑140 (MQ=255)
    tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGGGGTTGTTTTTTCATTGTTTTTTTTGTTGATATTTACGgttt                                                                                                                              >  1:6789929/1‑140 (MQ=255)
    tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTATTTGTTTTTGCATTATTTTTATTCTTGATATTAACGtttt                                                                                                                              >  1:7355316/1‑140 (MQ=255)
    tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGTCTTTCTTTTTGCAGTCTTTTTCTTATTCATATGAAAGtttt                                                                                                                              >  2:3986749/1‑140 (MQ=255)
    tttCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGGGCTTTTTTTTTACAAAGATTTTCTTGTTGATATGAACAtttt                                                                                                                              >  1:2140380/1‑140 (MQ=255)
       ccGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAg                                                                                                                           <  2:1116518/140‑1 (MQ=255)
        cGGATCAGATAGCAATTCATCGTTATGACTAATGTTTTCTTCAAGGGTTTGAACAGGGGCTTCAATTTCTTCACTCCGCCGGAGACGTTGGCGTTCTTTCTTTTTCCATTCATTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                          <  2:6602686/140‑1 (MQ=255)
        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                          <  2:5745011/140‑1 (MQ=255)
        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                          <  1:5771635/140‑1 (MQ=255)
        cGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                          <  2:1303678/140‑1 (MQ=255)
        cGGATCACATAGAAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGt                                                                                                                          <  2:6113307/140‑1 (MQ=255)
                 tAGCAATTCATCGTTATGACTAATGTTTTCTTCACTGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTaa                                                                                                                 <  2:6664200/140‑1 (MQ=255)
                 gaGCACTTCATAGTTATGACGAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCGTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGCACGTTTTGAGTCAGGCTTaa                                                                                                                 <  2:6456345/139‑1 (MQ=255)
                  aGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                                                <  1:7075673/140‑1 (MQ=255)
                  aGCAATTCATCGTTATGACTAATGTTTTCGTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCGTCATATGAACGTTTTGAGTCAGGCTTAAc                                                                                                                <  2:3359709/140‑1 (MQ=255)
                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTGTTTATTTTTGCATTGTTTTTGTTGTTCATATTTACGGTTTGATTGAGGGTTATACGGAGCa                                                                                                         >  2:7392948/1‑140 (MQ=255)
                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGGGGTTGTTTTTGGAGTGTTTTTTTTGTTGATTTTGACGGTTTTTGTTAGGCGTAACCgcagca                                                                                                         >  1:392389/1‑140 (MQ=255)
                         cATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTGTTTCGTGTTGGATTCTTTTTATTGTTGAGATGGACGTTTTGTGTGAGGGTTAAACGAAGCa                                                                                                         >  2:4249115/1‑140 (MQ=255)
                         cATCGTTATGACTAATGTTATCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCACTCCGACGGAGGCGTGGGGGTTCGTTCTGTTTGCCATCTTTTTGGACTGCATATGAACGTTTTGACTCAGGCTGAACAGACGCa                                                                                                         >  2:4684883/1‑140 (MQ=255)
                          aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAg                                                                                                        >  2:3538420/1‑140 (MQ=255)
                          aTCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGCGGTGTTTGTTTTTACAATATTTTTAGTCTTAATAGGTACGTTTTGAGTACGGCTTAACCGAATCAg                                                                                                        >  2:1509646/1‑140 (MQ=255)
                           tCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAgg                                                                                                       <  2:4148285/140‑1 (MQ=255)
                             ggtATGACGAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCGTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCg                                                                                                     <  2:5140134/138‑1 (MQ=255)
                              ttATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTGGTTTATTTTTGCATTATTTTTGTTGTTCATATTAACGGTTTGAGTCCGGGGTAACCGCAGCAGGcct                                                                                                    >  1:855786/1‑138 (MQ=255)
                                       aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGCGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                                           <  1:7469589/140‑1 (MQ=255)
                                       aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                                           <  2:2478121/140‑1 (MQ=255)
                                       aTGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCATGTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGa                                                                                           <  1:2445969/140‑1 (MQ=255)
                                        tGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAt                                                                                          <  2:5651787/140‑1 (MQ=255)
                                        ggTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGAt                                                                                          <  1:6388914/139‑1 (MQ=255)
                                          tgttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGATTAACCGAAGCAGGCGTTTTTTCGGATTc                                                                                        <  1:4104417/138‑1 (MQ=255)
                                            ttcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTAGTTTTCCATTCTTTTTGTTCTTCATATGAACGTTTTGAGTCAGGCTTTAACGAAGCAGGGGTTTTTTGGGAttaca                                                                                      >  2:569435/1‑137 (MQ=255)
                                             tcttcAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCCATATTTTTTTTGTTCATATTTAACGATTGTGTAAGGCTTTAAAGAAACACGCGGTTTTTTGGGTTGctc                                                                                     >  1:6168980/1‑140 (MQ=255)
                                                         tGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTGCTTTATTGTTCCATTCTTTTTGGTGTTCATATTAACGTTTTGAGTCAGGCTTAAACGAACCAGGCGTTTTTTCGGATTGCTCTTGCTGATTCAt                                                                         >  2:293863/1‑140 (MQ=255)
                                                          gAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCAtt                                                                        <  1:2537756/140‑1 (MQ=255)
                                                           aaCAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTGCATTATTTTTATTCTTAATATGAAAGTTTTTATTGAGGCGTAAACGAATCAGGCGTTTTTTCTGATTACTCTTTGGGATTAAttt                                                                       >  2:2192578/1‑140 (MQ=255)
                                                              aGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTtct                                                                    <  2:916668/140‑1 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTTGGGGTCTTTGTTTTTCCATGCTTTTTTTTGTTGATGTGGGGGTTTTGTGTGGGGCGTTACCGACGCGGGGGTTTTTTCGGCTTGCGCTTGCGGGTTCGttttgggg                                                                  >  2:6337533/1‑136 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGTTCTTTATTTTTGCATTGTTTTTCTTCTTGATATGTAAGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGCTTGCTTTTCTcc                                                                  >  2:5886288/1‑140 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTTTTTTTGTTGTTGATATGAAACTTTTTTGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGATTGCTCTTGGGTATTGAttttatac                                                                  >  2:1991978/1‑136 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTATTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTGAGGCTTAACCGAAGCAAGCGATTTTTAGAATAACTCATGATGATTAAATTtctaa                                                                  >  2:7166494/1‑138 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCGTTCTTTTTACAATCTTTTTATTCTTCATATGTACGTTTTGAGTCAGGCTTAACCCAAGAAGGCGGTTTTTCGGATTGATCTTGCTGCTTGATTTGCTcc                                                                  >  2:4437451/1‑140 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTGGTTTGTTTTTGCATTGTTTTTGTTGTTAATATTTACGTTTTGTGTCAGGCTTAACCGAAGCAGGCGTTTTTTAGGATTGCTCTTGGTGATTGATTTTCTcc                                                                  >  2:3474369/1‑140 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGGGGTGTTTGTTTTTGCATTCTTTTTATTGTTCATATTAACGTTTTGTGTCAGGCATTACCCAACCACGCGGTTTTTGGGGTTGCGCTTTCTGTTTGAttttgtgc                                                                  >  1:7564054/1‑136 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCGTTCTTTCGTTTTACAGTATTTTTGATCTTGAGATGAACCTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTAGGATTACTCTTTGTGAGTCATTTtcggc                                                                  >  2:6271666/1‑137 (MQ=255)
                                                                tGGCTTCAATTTCTTCAATCCGACGGAGTCGTGGGCCGTCATTATTTTTGCCTTGGTTTTAATCTTCAAATGCACCTTTTTATTAAACCTTAAACCAACGACGGCTTTTTTGGGATTGCTAGTGCTGGTTCAttttagga                                                                  >  2:3046157/1‑136 (MQ=255)
                                                                        aTTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                                                          <  2:7217541/140‑1 (MQ=255)
                                                                        aTTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAg                                                          <  1:5428298/140‑1 (MQ=255)
                                                                             ttcAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAgctgct                                                     <  2:3674128/140‑1 (MQ=255)
                                                                                  tCCGACGGAGTCGTTGGGGGTGTTTGTTTTTGCATTGTTTTTGTTGTTCATATGAACGTTTTGTGTCAGGCTTAAACGAAGCAGGCGTTTTTTCGGAGTCCGCTTGCTGGTTCATTTTATGCAATTCAAGCTGCGCTGtt                                                >  2:5246115/1‑140 (MQ=255)
                                                                                       cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                           <  1:3240617/140‑1 (MQ=255)
                                                                                       cGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGttttttt                                           <  1:6096514/140‑1 (MQ=255)
                                                                                         gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTACCCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                         <  2:4561245/140‑1 (MQ=255)
                                                                                         gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGCTTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                         <  2:2619421/140‑1 (MQ=255)
                                                                                         gAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTtct                                         <  1:5592624/140‑1 (MQ=255)
                                                                                          cgTCGTTGGCGTTCTTTAGATTCCCATTCTTGTTCTACTTCAAATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCACCCTGCTCTGTTTTTTtctc                                        <  2:5514775/139‑1 (MQ=255)
                                                                                            tCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCg                                      <  2:163832/140‑1 (MQ=255)
                                                                                                 ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa                                 <  1:4964862/140‑1 (MQ=255)
                                                                                                 ggCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTataa                                 <  2:3576088/140‑1 (MQ=255)
                                                                                                      tctttcttTTTCCATTCTTTTTCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAACCAGGCGTTTTTTAGGATTACTCTTGCTGTTTCATTTTATCCAAATAAATCTGCTATGTTTTTTTATACGTATaaaaata                            >  1:3685978/1‑139 (MQ=255)
                                                                                                          tctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTa                         >  2:2712604/1‑139 (MQ=255)
                                                                                                           ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                       <  1:1568126/140‑1 (MQ=255)
                                                                                                           ctttTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                       <  2:4047168/140‑1 (MQ=255)
                                                                                                           ctttTTACATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAAt                       <  2:4920833/140‑1 (MQ=255)
                                                                                                                tCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTccc                  <  2:5593963/140‑1 (MQ=255)
                                                                                                                   aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa               <  2:3171400/140‑1 (MQ=255)
                                                                                                                   aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa               <  2:5464923/140‑1 (MQ=255)
                                                                                                                   aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa               <  1:4835557/140‑1 (MQ=255)
                                                                                                                   aTTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTATCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGa               <  2:2295300/140‑1 (MQ=255)
                                                                                                                     tgtttttCTTATTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGGAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAATCTGCTCTGTTTTTTTATCCGTATAATAATCGTAATCTCAGAGATa             <  2:7577506/138‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCCTATGAACGTTTTGAGTCAGGCTGAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:5291920/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATCTGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:2078167/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:5063908/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:3552206/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:3494425/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:4676332/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:3538420/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:6734065/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:3783335/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:4588668/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:3001600/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:2531406/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:5859261/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:5924953/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:726074/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCGCCCAGATa             <  2:1447516/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGAGTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTACTCTCCCAGATa             <  1:1021009/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATGCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  2:5390616/140‑1 (MQ=255)
                                                                                                                     tCTTTTTCGTCGTCATAGGAACGTTTGGCGTCAGGCTTACCCGAAGCAGGCGTTTGTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATa             <  1:7687439/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATCTGAAAGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  2:2899877/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGACGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:7038622/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:2850875/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:4028739/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:1991978/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:571669/140‑1 (MQ=255)
                                                                                                                       tttttCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATAct           <  1:1105423/140‑1 (MQ=255)
                                                                                                                       ctgttcttctgcATCTGAACGTTGTGAGTCAGGCGTAACCGAAGCAGGCGTGTTTTCGGAGTCCGCTGGCTGATTCATTTTCTCCAATGCAAGCTTCTCTGTTTTTTGCTCCGTATAATCATCGTACTCTCCCAGATAct           <  2:3915858/137‑1 (MQ=255)
                                                                                                                              tcttcATATGAACGTTTTGAGTCAGGCTTAACCGACGCATGCGTTTTTTCGGATGCCTCTTGCTGATTCTTTTTCTCCAATTCAACCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat    <  1:4240773/140‑1 (MQ=255)
                                                                                                                              tcttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat    <  1:1495798/140‑1 (MQ=255)
                                                                                                                              tcttcATAAGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtat    <  2:6919664/140‑1 (MQ=255)
                                                                                                                               cttcATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCtata   <  1:4465380/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                 
TAGATTTCCGGATCACATAGCAATTCATCGTTATGACTAATGTTTTCTTCAATGGTTTGAACAGTGGCTTCAATTTCTTCAATCCGACGGAGTCGTTGGCGTTCTTTCTTTTTCCATTCTTTTTCTTCTTCATATGAACGTTTTGAGTCAGGCTTAACCGAAGCAGGCGTTTTTTCGGATTCCTCTTGCTGATTCATTTTCTCCAATTCAAGCTGCTCTGTTTTTTTCTCCGTATAATAATCGTAATCTCCCAGATACTCTTCTATAT  >  NZ_CP034943/633951‑634218

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: