Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 755,531 A→C 10.8% Q22H (CAA→CAC)  EO946_RS03880 → rhamnogalacturonan acetylesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943755,5310AC10.8% 967.8 / 20.1 416Q22H (CAA→CAC) EO946_RS03880rhamnogalacturonan acetylesterase
Reads supporting (aligned to +/- strand):  ref base A (254/93);  new base C (4/63);  total (258/158)
Fisher's exact test for biased strand distribution p-value = 2.86e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755393‑755669
                                                                                                                                          |                                                                                                                                          
   ttttttttCAAGTTAAGATCTGTCCATTATTGATTACATATTAAACGAAAATAACGAGTTTGAATTAGAAATGGCAAATCACATTTATCTTGCTTGCGATTCAACTGTTCAAACTTATGGAGACAGCACAAATCAAgggg                                                                                                                                        <  2:5359617/138‑1 (MQ=255)
     ttttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAgggggg                                                                                                                                      >  1:4583208/1‑140 (MQ=255)
       ttttCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTg                                                                                                                                    >  1:7259820/1‑140 (MQ=255)
           cAAGTGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAATCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTggggg                                                                                                                                <  2:4350678/140‑1 (MQ=255)
             aGGGAAGATCTTTCCATGATTGATGCCATATTAACCGAAAAAAACGAGGGTGAATGAGAAATGGCTAATCAGATTTATATTGCTGGGGATTCAACTGTTCAAACGTATTGAGACAGCACAACTCACGGGGGGTGGGGGCa                                                                                                                              <  2:2996156/140‑1 (MQ=255)
                   gagCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACCGCACCAATCAAGGGGGGTGGGGGCAGTTtct                                                                                                                        >  2:2962274/1‑140 (MQ=255)
                        gTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCt                                                                                                                   >  1:5922239/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGACTGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:5684900/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCCGTTTCTCGGCTc                                                                                                                  >  2:6384587/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:197870/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  2:482458/1‑140 (MQ=255)
                         tCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTc                                                                                                                  >  1:2759146/1‑140 (MQ=255)
                           cATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAg                                                                                                                                           >  1:6040895/1‑113 (MQ=255)
                           cATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGc                                                                                                                >  2:3691471/1‑140 (MQ=255)
                                ttgatTCCATATTAAAAGAAAAAAAGGTTGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGCGGGGGGGGGGCAGTTTCTCGGCTCGCATCTg                                                                                                           <  2:6754166/140‑1 (MQ=255)
                                  gatGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGcc                                                                                                         >  1:4143896/1‑140 (MQ=255)
                                   atGCCATATGACACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAAATGTGCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:6591902/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGCATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTCCCg                                                                                                        >  1:3993618/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATGTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:1197592/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:7049735/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:6332503/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  2:2645501/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:5349495/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:5244741/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:1004670/1‑140 (MQ=255)
                                   atGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        >  1:1375817/1‑140 (MQ=255)
                                   atGCCATATGAAACCAAAAAAACGAGGGCGAAGGCGAAATGGCAAATCACATTTATCTTGCTGGCGATTCACCGGTTCAAACGTCGGGCGACAGCACAACTCCAGGGGGCTGGGGGCAGTTTCTCGGCTCGCATCTGCCg                                                                                                        <  1:6486941/140‑1 (MQ=255)
                                     tacATATGAAATGACAAAATCGAGCGGTAATGATAAATGGCAAATCACATTTATCTTGCTGGCGATTCCACTGTTCAAACGTCTGGAGACAGCACAAAACAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  2:7546900/138‑1 (MQ=255)
                                     gacATATGAAACGAAAAAAACGAGGGGGAATGACAACTCGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACACAGCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  1:150216/138‑1 (MQ=255)
                                     gCCATCTGAAACGACACAAACGAGCGCGAATGAGAAAGCGCAAATCACATTTATCTTGCTGGCGATTCACCTGTTCAAACGTATGGAGACAGCACCCACCCAGGGGGGTGGGGCCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  2:1550575/140‑1 (MQ=255)
                                     gCCATATTAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAACTCACATTTATCTTGCTGGCGATTCAACTGTACAAACGAATGGAGACAGCCCACATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  1:3092812/140‑1 (MQ=255)
                                     gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACTCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      <  2:3083317/140‑1 (MQ=255)
                                     gCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGGATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGa                                                                                                      >  1:4777602/1‑140 (MQ=255)
                                       aatatGACAAGAACAAAAGTAGGGTGAATGATAAATGTAAAATCTCTATTTTCTTGCTGTATATTCAACTGTACAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGc                                                                                                    <  2:5243341/139‑1 (MQ=255)
                                        atatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCa                                                                                                   >  1:3624291/1‑140 (MQ=255)
                                        atatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCa                                                                                                   >  1:7223266/1‑140 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCTATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                  >  2:3270137/1‑140 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                  >  1:5529851/1‑140 (MQ=255)
                                         tatGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCat                                                                                                  >  2:1320641/1‑140 (MQ=255)
                                             acaccaaaaaaaCGAGGGGTACTGAGAAATCCCAACTCACATTTATCTTGCTGGCGATTCACCTGTTCACCCGTCTGGAGACAGCACACAACCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:3319364/135‑1 (MQ=255)
                                             aaaaGAAAAAAACGAGGGGTAATGTGAAATGGCAAATCACATTTATCTTGCTGTCTATTCAACTGTTCAAAATTATGGAGACAGCACAAATCTAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:6215734/140‑1 (MQ=255)
                                             aaaCTAAATTAACGCTGGGGAATGAGAAATTGCAAATCACATTTATCTTGCTGGCGATTCAACTTTTCAAACGTATGGAGACAGCACAAAACATGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:2300718/140‑1 (MQ=255)
                                             aaaCTAAAAACACGTGGGTGAATGATAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAACCCCCGCGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:1727215/140‑1 (MQ=255)
                                             aaaCTAAAAAAACGAGGTGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  2:2031601/140‑1 (MQ=255)
                                             aaaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTc                                                                                              <  1:6947391/140‑1 (MQ=255)
                                              aaCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCa                                                                                             >  2:3325367/1‑140 (MQ=255)
                                               actaaaaaaaCGAGGGTGAATGAGAATTTGAAAATCATATTTATCTTGATTGTGATTCAAATTTTCATTCTTATGGAGTCAGGACAAATCAAGTGGGGTTTGGGCAGTTTATCGGCTCGCATCTGCCGGAGCATATTCat                                                                                            <  2:4505501/137‑2 (MQ=255)
                                               actaaaaaaaCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCATCTGTTCACACGTATGGAGACAGCACAACTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  2:5137974/137‑1 (MQ=255)
                                               accataaaaaGGAGGGGTAATGAGAATTGGAATATCACTTTTATCTTGCTGGCGATTCCACTTTTCATACGTATGGAGAAAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTcta                                                                                            <  2:7450978/135‑3 (MQ=255)
                                               accaaaaaaaCGAGGGGGAATGAGAAATCGCAAATCACATTTATCTTGCTGGCGATACAACTGTTCCACCGTATGCAGACACCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  1:512541/137‑1 (MQ=255)
                                               aCGAAAAAAACTCTGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACTTATGGAGACAGCACAAATCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  2:3380075/140‑1 (MQ=255)
                                               aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            >  1:779894/1‑140 (MQ=255)
                                               aCGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            >  1:21647/1‑140 (MQ=255)
                                               aCGAAAAAAACGAGGGGGAAGCACAAATGCCAACTCACTTTTATCTTGCGGGCGATTCAACTGTTCAAACGTATGCAGACAGCACACCTCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCaa                                                                                            <  1:2645501/140‑1 (MQ=255)
                                                  aaaataaCTAGGTGTAATTAGAAATGTCTAATCACATTTATCTTGCTGTCTATTAAACTCTTCATACGTATGTAGACAGCACAAATCAAGGGGGGTGGTGGCAGTTTCTCGGCTCGCATCTGACGGAGCATATTCAAGTg                                                                                         <  2:5959700/140‑1 (MQ=255)
                                                       aaCGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCGACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTaa                                                                                    <  2:4586180/140‑1 (MQ=255)
                                                            gggggATTCAGAAATGCCAAATCTCATTTATCTTGCTGGCGATTCAACTGTTCAACCGTATGCAGACCGCACAACTCCCGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagag                                                                               <  1:4925250/140‑1 (MQ=255)
                                                            gagctaaTGAGATATGCCAAATCAAATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACagag                                                                               <  2:6671182/135‑1 (MQ=255)
                                                               ggAATGACAACTGGCAAATCACCTTTATCCGGCTCGCGCGTCAACTACTCAACCCTAACGAGCCAGCACAACCCACGGCGGCCGCGGCCAGGTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCaa                                                                            <  1:887520/140‑1 (MQ=255)
                                                                gaagtagaAATCGCAAATCACATTTATCTGGCTGGCGAATCAACTGTCCCAACGTCTGGCCCCAGCACCCCTCCCGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAAt                                                                           <  2:2500262/135‑1 (MQ=255)
                                                                           ggCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGgaagaa                                                                >  2:3425799/1‑140 (MQ=255)
                                                                                     ttttATGTTTGTGGCTTTTCAACTTTTCAAACGTATGGAGACTGCACAAATCACGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                      <  2:6428162/139‑1 (MQ=255)
                                                                                     aTTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAAc                                                      >  2:4735506/1‑140 (MQ=255)
                                                                                               cgggCGCTCCAACTGTGCAACCCGAGGGACCCAGCACAACTCCAGGGGGGGGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            <  2:2532960/138‑1 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  1:1843793/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  2:5724953/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  1:5455546/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  1:3893709/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  2:6040550/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            >  2:4120160/1‑140 (MQ=255)
                                                                                               cTGGCGATTCAACAGTTCAAACGGACCCACACACCACCACCCCCGGGGGCTCGGGCCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGaa                                            <  1:1024713/140‑1 (MQ=255)
                                                                                                 ggCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGACGAAGCTCGAAAACATTTGTGGAaga                                          >  2:82108/1‑140 (MQ=255)
                                                                                                        cAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAgg                                   >  1:2532960/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:1261485/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:1573731/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:3854253/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  2:4957756/1‑140 (MQ=255)
                                                                                                              ttCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATACAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAg                             >  1:615021/1‑140 (MQ=255)
                                                                                                                cacacctATGGACACACCACAACTCAAGCGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           <  2:2742437/134‑1 (MQ=255)
                                                                                                                cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           >  1:6618739/1‑140 (MQ=255)
                                                                                                                cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           >  1:2327030/1‑140 (MQ=255)
                                                                                                                cAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGc                           >  1:711164/1‑140 (MQ=255)
                                                                                                                    cttATGGAGACAGCACAAATCAAGGGGGGTGGGGGAAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGAtt                       <  2:6104761/138‑1 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:6720042/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  2:5062967/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:4374713/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:7524713/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  1:3494170/1‑140 (MQ=255)
                                                                                                                        tGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTg                   >  2:28103/1‑140 (MQ=255)
                                                                                                                         ggAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTATCGGCTCGCATCTGCCGGAGCATATTCAAGTGTTTAACAGAGCAATCTGGGGAAGAAGCTCGAAAACATTTGTTGAAGTGGGCAGGCTTCATGCGATTCTTGa                  >  2:5474719/1‑140 (MQ=255)
                                                                                                                         ggAGACACCACAAATCAAGGCGGGAGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTACCAGACCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGa                  <  2:7254058/140‑1 (MQ=255)
                                                                                                                         cgagaCAGCACACAGCCCGGGCGGGGCGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGa                  <  2:3941039/139‑1 (MQ=255)
                                                                                                                         cgagaCAGCACAACTCACGGGGGGTGGGCGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGa                  <  2:1015298/139‑1 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCACTCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:7342772/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  2:3580625/1‑140 (MQ=255)
                                                                                                                               aGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGAt            >  1:2927937/1‑140 (MQ=255)
                                                                                                                                 cacaAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTg          >  1:2426753/1‑140 (MQ=255)
                                                                                                                                      aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGCGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTGGTGGAAGAGGGCAGGCTTCAGGCGAGACTTGATGTGATTGAGCCg     >  2:1829071/1‑140 (MQ=255)
                                                                                                                                      aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCg     >  2:7032882/1‑140 (MQ=255)
                                                                                                                                      aTCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGAGGTGATTGAGCCg     >  2:4632745/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                          
ACCGGTTTTTTCAAGGGAAGAGCTGTCCATGATTGATGCCATATGAAACGAAAAAAACGAGGGGGAATGAGAAATGGCAAATCACATTTATCTTGCTGGCGATTCAACTGTTCAAACGTATGGAGACAGCACAAATCAAGGGGGGTGGGGGCAGTTTCTCGGCTCGCATCTGCCGGAGCATATTCAAGTGATTAACAGAGCAATCGGGGGAAGAAGCTCGAAAACATTTGTGGAAGAGGGCAGGCTTCAGGCGATTCTTGATGTGATTGAGCCGGAT  >  NZ_CP034943/755393‑755669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑