Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 112,586 G→T 6.9% intergenic (+264/‑40) gltX → / → cysE glutamate‑‑tRNA ligase/serine O‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943112,5860GT 6.9% 1789.9 / 20.8 721intergenic (+264/‑40)gltX/cysEglutamate‑‑tRNA ligase/serine O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (246/395);  new base T (73/6);  total (319/402)
Fisher's exact test for biased strand distribution p-value = 1.64e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 732 total aligned reads displayed.

TTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACA  >  NZ_CP034943/112448‑112726
                                                                                                                                          |                                                                                                                                            
ttCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTgg                                                                                                                                             >  2:6353968/1‑140 (MQ=255)
   gAAATCAGTATTCATAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAAGAGATTGGAACCGCGCGGTTTAAGCGTATCTTTAATGTTTTAATTATTATTCGCTTTTTTTATTTGGTg                                                                                                                                          <  2:2857034/140‑1 (MQ=255)
     aaTCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTTaa                                                                                                                                        >  2:454751/1‑140 (MQ=255)
                 aaGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCaaa                                                                                                                            <  1:4795690/140‑1 (MQ=255)
                   ggcggaCGCCGCCGTTACAGTCTGAATTTGTGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGAGGTTTAAGCGTCTCTGTGATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTTAAGAGGAAATCAAAtt                                                                                                                          <  2:1792307/140‑2 (MQ=255)
                     cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATaga                                                                                                                        >  2:5126891/1‑140 (MQ=255)
                     cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATaga                                                                                                                        >  2:5334972/1‑140 (MQ=255)
                     cggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTAAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATaga                                                                                                                        >  2:2674000/1‑140 (MQ=255)
                      ggcCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATagag                                                                                                                       <  1:1132801/140‑1 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAgaga                                                                                                                      >  1:3519645/1‑140 (MQ=255)
                       gccgccgccGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGTAGAGGAAATCAAATAgaga                                                                                                                      >  1:2566570/1‑140 (MQ=255)
                        ccgccgccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGaa                                                                                                                     <  1:5320062/140‑1 (MQ=255)
                              ccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                                                                               <  2:7691444/140‑1 (MQ=255)
                              ccgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTgggg                                                                                                               <  2:4975580/140‑1 (MQ=255)
                              ccgTGACAGGCTGAAGTTGGGAGAGCATAAGTCTGCTTTTCAAACATAGTGGAACCGGGAGGGTTAAGCGTCTCTTTCATGTTTTGATGCAGAGGCGGTTTTTTTATTGGGTGAAGAGGAAATCATATAGAGAAGTgggg                                                                                                               <  2:5942957/140‑1 (MQ=255)
                               cgTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGGGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTggggg                                                                                                              <  2:4816710/140‑1 (MQ=255)
                               cgTTACAGGCTGAAGTTGGGAGAGCAGAACTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTggggg                                                                                                              <  2:4429762/140‑1 (MQ=255)
                                      ggCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATATATAAGTGGGGGGAAGCag                                                                                                       >  1:4952505/1‑139 (MQ=255)
                                         tGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAATCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAtgtg                                                                                                    >  2:7173509/1‑140 (MQ=255)
                                         tGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGGGGGGGGAAGCAtgtg                                                                                                    >  1:3457949/1‑140 (MQ=255)
                                                 ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGTGGCGCTTTTTTTATTGGGTGTAGTGGAAATAAAAAAGATTAGTGGGGGGAAGCATGGGTTTTTTAg                                                                                            >  1:6497666/1‑140 (MQ=255)
                                                 ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGATGAAAACAAATAGAGAAGTGGGGGGAAGCATGTTTTTTTTAg                                                                                            >  2:608821/1‑140 (MQ=255)
                                                 ggAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAg                                                                                            >  2:6684550/1‑140 (MQ=255)
                                                  gagcccAGACGTCTGCATTTCAACCAGAGTGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                           <  2:6193708/135‑1 (MQ=255)
                                                  gagaGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTACGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGa                                                                                           <  1:2813789/140‑1 (MQ=255)
                                                     agCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAGGTGGGAATCAAATAGAGAATGGGGGGGCAGCAGGTGtgtttttatatg                                                                                        >  1:6420724/1‑134 (MQ=255)
                                                       cAGAAGTCTGCTTTTCAAACCGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGCAGAGGAAATCAAAGAGAGCAGAGGGGGGAAGCAGGGGTTTTTTAGaagggt                                                                                      >  2:5817281/1‑136 (MQ=255)
                                                       cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCt                                                                                      >  2:5263325/1‑140 (MQ=255)
                                                       cAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGTAAGAGGAAAACAAATAGAGAACTGGGGGGAAGCATTTGTTTTTTAGAATTCt                                                                                      >  2:5442499/1‑140 (MQ=255)
                                                          aaGTCTGCTTTTCAAACAGAGTGGACCCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGTAGAGGAAATACAATAGAGAAGTGGGGGGGAGCAAGTGTTTTTTATAAATCTCaa                                                                                   >  1:1382113/1‑140 (MQ=255)
                                                            gTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaag                                                                                 <  1:6299730/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAACTCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  1:7668687/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  2:1380962/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  1:7480931/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  1:1637390/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  1:5833797/140‑1 (MQ=255)
                                                              cTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTCTTTATTGGGTGAAGAGGAAAACAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAaagaa                                                                               <  1:6821680/140‑1 (MQ=255)
                                                               tGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGAGTCTCTGTGATGTGTTCATGCAGAGGCGCTTTTTGTAATGGGGGTAGAGGAAATCGCATAGAGAAGTGGGGGGACGCATGTGTTTTTTCTGATGCTCAaagaag                                                                              >  2:1193896/1‑140 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAATCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAACTAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  2:3935039/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCCTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCCAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  1:2982863/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  2:4709533/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  2:2340582/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  1:4064234/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  1:2738659/140‑1 (MQ=255)
                                                                gCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCGGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  2:420714/140‑1 (MQ=255)
                                                                 cTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATAAAATAGAGCACTGGGGGGGACCATGTGTTTTTTATAAAGCTCaaaaaaaaa                                                                            >  1:5206163/1‑137 (MQ=255)
                                                                  ttttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACa                                                                           <  2:5558174/140‑1 (MQ=255)
                                                                  ttttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACa                                                                           <  2:3519645/140‑1 (MQ=255)
                                                                  ttttCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTCTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGCAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACa                                                                           <  2:7306713/140‑1 (MQ=255)
                                                                        aaCAGAGGGGAACCGCGCGGTGAAAGCGTCTCTGTCATTTTTTCATGCAGTGGCTCTTTTTTTATTGGGTGAAGAGTAAATCAAATAGAGAACTGGGGTGAAGCTTGTGTTTTTTAGAATGCTCAAAGAAGACATTGATa                                                                     <  2:6711863/140‑1 (MQ=255)
                                                                            gagTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAAAAGAGAATTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAgt                                                                 >  2:1920287/1‑140 (MQ=255)
                                                                             agTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAAAGCTCAAAGAATACATTtataaaatg                                                                >  2:2497983/1‑135 (MQ=255)
                                                                                   aCCGCGCGGTTTAAGCGTCTCTTTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGCAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGGGTTCGAt                                                          <  1:4596829/140‑1 (MQ=255)
                                                                                     ctcgaggTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTCTTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCa                                                        <  2:2242830/135‑1 (MQ=255)
                                                                                     cgcgcgGTTTAAGCGTCTCTGTCCTGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCa                                                        <  1:3231233/140‑1 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCTCTTTTTTTATTGGGTGTATAGGAAAAAAAATAGTGAATAGGGGTGTAACATGTGTTTTTTAGAATGCTCAAATAAAAAATTGTTACAGTTTTCGATCaaa                                                      >  2:2102840/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGTATAGGAAATCAAATATAGTAGTGGGGGGGAGCATGTGTTTTTTAGAATTCTCAAAGAATACATTTTTAAAGTGTTCGATAAAg                                                      >  1:5363580/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGAAGAGGAAATAAAAAATAGTAGTGGGGGGGAGCCAGTGTTTTTTATAATTCTCAAAGAAGAAAAAGATTCAGTGTTCGATaaaa                                                      >  1:7256647/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAATAGGAAAACAAATAGAGAACTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATAAAGTGTTCGATCaat                                                      >  2:7027052/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGTAATCAAATAGAGAAGTGGGGGGGAGCCAGTGTTTTTTTTTATGCTCAAAGTATAAATTGTAACAGTGTTCGATCAAg                                                      >  1:7090987/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGTGAAGAGGTAATAAAAAATATAATGGGGGGGACGAATGTTTTTTTTTTTATTGTGAATTATTACATTGATAAAGTGTTAGATGAAg                                                      >  1:3802163/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTTGGGGTACTTGTAATAAAAAAGAGAAGTGGGGGGTACCATGTGTTTTTTAGAATTCTAAAAAAAAAAAAATATAAAATGTTCGATCaaa                                                      >  1:1829695/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGTAGAGGAAATGAAATAGAGAAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTTATCCAGTGTTCGATCAAg                                                      >  2:6856469/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGCGGAAATCAAATAAATAAGGGGGGGGAAGCATGTGTTTTTTAGTATTCTAAAACAAGAAAATTATACAATGTTATATCaaa                                                      >  1:4446568/1‑139 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGCAGCATGTGTTTTTTAGAATGCTCAAATAAGACATTTATACAGTGTTCGATAAAg                                                      >  2:1465720/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAg                                                      >  2:4299050/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGGGTAGAGGAAATCAAATAGAGCAGTGGGGGGAAGCCTGTGTTTTTTAGAAAGCGCAAAGAAGACATTGTTACAGTGTTAGATCAAg                                                      >  1:5665082/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTTTTTGGGGGAGATGCAATCAAATAGAGAAGGGGGGGGGCGGCTGTGTTTGTTAGAATGCTGAAAGAATACAATGGAGCAGGGTTGGATGAAg                                                      >  1:5768877/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGGGGAGACGGAAACAAATACAGAAGGGGGGGGCACCAAGGGTTTTTTTTTATTCTCAAAGAAAAACATGTTAAAGTGTTAGATGAAg                                                      >  1:6906647/1‑140 (MQ=255)
                                                                                       cgcgGTTTAAGCGTCTCTGTCATGTTTTAATGCAGAGGCGCTTTTTTTATTGGGTGTAAATGGAATCAAATAGATTAGTGGGGGGAAGGATGTGTTTTTTTGAATTCTCAAAGAATAAATTTATAAATTGGTCGATaaaa                                                      >  1:3674201/1‑139 (MQ=255)
                                                                                         cgGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTGGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGAt                                                    >  2:1062127/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGTAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCAGGTGTTTTTTATAATGCTCAAATAAGAAATTGATACAGTGTTGGATCAAGATACAGCACCaaaaa                                       >  1:5948039/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTTTTTGGTGAAGAGGAAATCAAATAGATAAGTGGGGGGAAGCAGGTGTTTTTTTTAATTCTCAAATAAGACATAGATAAAGTGTTGGATCAAGATCCAGCAGCaagaa                                       >  1:2046336/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGGATATGTAATAAAAAAGAATAGTGGGGGGAAGGAAGTGTTTTTTTGGAATCTCAAAAAATACAATTATAAAATTTTGGATAAAGATACAGCATCaatta                                       >  1:4266816/1‑137 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAATAGGAAATACAATAGAGAAGTGGGGGGCAGCATGTGTTTTTTACAATGCTCAAAGAATACATTGATACAGTTTTCGATCAAGATACAGCAGCaagaa                                       >  2:6418796/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGCTGAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTAGATAAAGATACAGCCGCaagaa                                       >  2:6534291/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAATCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCATCAGCaagaa                                       >  2:7495237/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTATAATGCTCAAAGAAGACATTGATACAGTGTTCGATAAAGATCCAGCAGCaataa                                       >  2:3716697/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAagaaga                                                                             <  1:1024141/102‑1 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGAt                                                          <  1:6138200/121‑1 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAACCATGTGTTTTTTATAATTCTCAAAGAAGACATTGATAACGTGGTCGATCAATATCCAGCAGCaagaa                                       >  2:5714012/1‑140 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGACACGTGGGGGGAAGCCTGTGTTTTTTAGAATTCTCAAAGACGACAATGATAACGGGTTCGTTAAAGATCCAGCAGCaagta                                       >  2:3269381/1‑138 (MQ=255)
                                                                                                      tctGTCATGTTTTCATGCAGAGGCGCGTTTTTTATTTGGTGGAGTGGTAATCAAATAGTGAAATGGGGGGACTCATGTTTTTTTTAGAAATGTCAAATAATAAAATTATAAATTGTTGGATACATATACAGcaacaaaac                                       >  1:7577528/1‑137 (MQ=255)
                                                                                                               ttttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGCAATCAAATAGAGAAGTGGGGGGAAGGATGTGTTTTTTATAATGCTCAAAGAATACATTGATACAGTGTTCGATCAAGATACAGCAGCAAGAAGTTAttttt                              >  2:29410/1‑139 (MQ=255)
                                                                                                                 ttCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                            <  1:2317342/140‑1 (MQ=255)
                                                                                                                 ttCATGCAGAGGCGCTTTTTTTATGGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGaa                            <  2:1265154/140‑1 (MQ=255)
                                                                                                                  tCATGCCGAGGCGCGTTTTTTATTGGGTGGAGAGGAAATCAAATAGAGTAGTGGGGGGTAGCCTGTGTTTTTTAGAATGCTCAAAGAAGACAATGATACAGTGTTCGATAAAGATCCAGCAGCAAGAACTTATTTTGaat                           >  2:6959235/1‑139 (MQ=255)
                                                                                                                  tCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATTCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAg                           <  2:1545010/140‑1 (MQ=255)
                                                                                                                    aTGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAgcagca                                           <  1:5488939/122‑1 (MQ=255)
                                                                                                                          agGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTa                   <  1:1215235/140‑1 (MQ=255)
                                                                                                                                 ttttttATTGGGTGAAGAGGAAATCAAATAGAGACGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTAtt            <  2:874227/140‑1 (MQ=255)
                                                                                                                                   ttttCTTGGCTGAAGAGGAAATCACCTAGAGAAGGCGGGCGCAGCATGTGTTTTAGAGCATGCTCAACGCAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTGTAACTTATTcc          <  1:5564264/140‑1 (MQ=255)
                                                                                                                                    tttATTGGGTGAAGCTGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCg         <  2:7585313/140‑1 (MQ=255)
                                                                                                                                      tATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGt       <  2:3053543/140‑1 (MQ=255)
                                                                                                                                         tGGGTGAATAGGAAATCAAATAGACAAGTGGTGGGAATCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTTATTTTAACTTATTCCGGTTTa    <  2:1997358/140‑1 (MQ=255)
                                                                                                                                         tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa    <  1:966254/140‑1 (MQ=255)
                                                                                                                                         tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa    <  1:6693365/140‑1 (MQ=255)
                                                                                                                                         tGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa    <  2:3368267/140‑1 (MQ=255)
                                                                                                                                         tGGGTGAAGAGGAAATCAAAGAGAGCAGGGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTa    <  1:3925787/140‑1 (MQ=255)
                                                                                                                                          gggTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTAc   >  2:40718/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TTCGAAATCAGTATTCAAAGGCGGCCGCCGCCGTTACAGGCTGAAGTTGGGAGAGCAGAAGTCTGCTTTTCAAACAGAGTGGAACCGCGCGGTTTAAGCGTCTCTGTCATGTTTTCATGCAGAGGCGCTTTTTTTATTGGGTGAAGAGGAAATCAAATAGAGAAGTGGGGGGAAGCATGTGTTTTTTAGAATGCTCAAAGAAGACATTGATACAGTGTTCGATCAAGATCCAGCAGCAAGAAGTTATTTTGAAGTGATTTTAACTTATTCCGGTTTACA  >  NZ_CP034943/112448‑112726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: