Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 762,385 T→G 7.4% intergenic (+17/‑38) EO946_RS03895 → / → EO946_RS03900 extracellular solute‑binding protein/sugar ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943762,3850TG 7.4% 808.9 / 17.8 293intergenic (+17/‑38)EO946_RS03895/EO946_RS03900extracellular solute‑binding protein/sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (33/228);  new base G (29/2);  total (63/230)
Fisher's exact test for biased strand distribution p-value = 1.75e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

ACACAATTTATCATTGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCTTTATCTTCTT  >  NZ_CP034943/762246‑762524
                                                                                                                                           |                                                                                                                                           
acacAATTTATCATTGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGt                                                                                                                                             <  1:4219031/140‑1 (MQ=255)
     aTTTATCTTTGGAGAAATTTACGAAAATGGGTTCGAGGTTGGGGTGTAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGTTGAAGGACTTGAACAAATTGCATCAATCTTCAAAATAAACCGGTGCGCCTGCCGTTCGGc                                                                                                                                        <  2:2657458/140‑1 (MQ=255)
        gaTCATTGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGTAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAgg                                                                                                                                     <  2:7489900/139‑1 (MQ=255)
         aTCATTGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAACAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAATTAAACCGGTGCGGCTGCAGGTCGGCACgc                                                                                                                                    >  2:4333068/1‑140 (MQ=255)
          tCAGTGGAGAAATTGCCCAAAACGGTTTCGCGGATGCGGTGGAGCTTTGCAAAAAACCCGGCGGCAATGACCTGATGAAGGACTTGAACAAATGGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGgcg                                                                                                                                   <  1:5680696/140‑1 (MQ=255)
          cctttGGAGAAATTGACGAAAAAGGGTTCTTGGTTGCGGTGGAGCTTTGCAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAAAAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGgcg                                                                                                                                   <  1:5171774/137‑1 (MQ=255)
              tGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCGGCCGTTAGGCAGGCGCAca                                                                                                                               >  2:1607938/1‑139 (MQ=255)
              tGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCAGCCGTTCGGCAGGCGCACt                                                                                                                               >  2:3140062/1‑140 (MQ=255)
                gagaAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCAGCCGTTCGGCAGGCGCACTgg                                                                                                                             >  2:7588120/1‑140 (MQ=255)
                 ataatttGACGAAAACGGTTTCGAGGATGGGGTGGAGATTTGTAAAAAAAGCGGCGGCAATGACCTGATGAAGGAATTGAACAAATTGCATCAATCGTCAAAATAAACCGGGGCGCCTGCCGTTCGGCAGGCGCACTGGt                                                                                                                            <  1:2584598/135‑1 (MQ=255)
                 ataaaTTGACTAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGGAATGACCTGATGAAGGACTTGAACAATTTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGt                                                                                                                            <  2:5255883/138‑1 (MQ=255)
                   aaaTTGACTAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTAc                                                                                                                          <  2:6452012/140‑1 (MQ=255)
                   aaaTTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTAc                                                                                                                          <  2:5098365/140‑1 (MQ=255)
                     aTTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGGGCCAGCCGGTGGGCAGGCGCACAGGTaacc                                                                                                                        >  1:200017/1‑137 (MQ=255)
                     aTTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCAGCCGTTCGGCAGGCGCCCTGGTAccc                                                                                                                        >  2:2109904/1‑139 (MQ=255)
                          tgAAAAAGGGTTCGATGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGAACTGATGAAGGACTTGAACAAATTGTACCAATCGTCAAAATAAACCGATGCGCCTGCCTTTCGGCGGGCGCCCCGGTATCACGaaa                                                                                                                   >  1:6444736/2‑140 (MQ=255)
                           gAAAATGGGTTCTTGTTTGGGGTGTTGTTTTGCAAAAAAAGCGGCGGCAATGACCTTATTAAGTACTTGAACAATTTGCATAAAGCGTCAAAATAACCCGGTGCGCATGCGGTTGGGCAGGCGCACTGGTACCACGAAAg                                                                                                                  <  1:1027370/140‑1 (MQ=255)
                           gAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAg                                                                                                                  <  1:1594414/140‑1 (MQ=255)
                             aaaaGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTGGGCACGCGCACTGGTACCCCGCAAggc                                                                                                                >  2:5191858/1‑139 (MQ=255)
                              aatgggTGCGAGTCTGGGTTGGAGTTTTGCAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGgag                                                                                                               <  1:465248/137‑1 (MQ=255)
                                aGGTTTCGTGTATGCGGTGGAGTTTTGGAAAAAAAGCGGCGGAAATGACCTGATGAAGGAATTGAAAAAATTGCATCAATCGTAAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGaa                                                                                                             <  1:1428483/140‑1 (MQ=255)
                                aGGTTTCGAGGATGCGGTGGAGATTGGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGTTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGaa                                                                                                             <  1:6877822/140‑1 (MQ=255)
                                aGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGaa                                                                                                             <  2:4628781/140‑1 (MQ=255)
                                aGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGaa                                                                                                             <  2:5380528/140‑1 (MQ=255)
                                  gggacGAGGATGCGCTGGAGCTTTGGAAAACCCGCCGCGGCAATGACCTGCTGAAGGACTTGAACAAATAGCATCAATCGTCAAAACACACCGGTGCGCCCGCCGTTCGGCAGGCGCACTGGTACCCCGAACGGAGAACa                                                                                                           <  2:2825981/136‑1 (MQ=255)
                                  ggTTCGCGGTTGGGTTGGAGTTTTGTAAAAAAAGCGGCGGAAATGACCTGATGAAGGACTTGAAAAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  1:2988697/140‑1 (MQ=255)
                                  ggTTCGCGCATGCGGTGGAGCTTTGTAAAAAACGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  1:6089514/140‑1 (MQ=255)
                                  ggTTCGAGGATGGGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:3672256/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGTAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:1247614/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  1:4876838/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:2584144/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:4935157/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:5392298/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  1:7467438/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:6719827/140‑1 (MQ=255)
                                  ggTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACa                                                                                                           <  2:892263/140‑1 (MQ=255)
                                    ttCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGa                                                                                                         <  2:6351114/140‑1 (MQ=255)
                                            tGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGaaa                                                                                                 <  2:1017480/140‑1 (MQ=255)
                                              cGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCCTTCGGCAGGCGCACGGGTACCCCCAAAGGAGAAAAGATATGGaaaaa                                                                                               >  2:7131568/1‑138 (MQ=255)
                                                 tGGAGTTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGAATTGAAAAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTg                                                                                            <  1:6068563/140‑1 (MQ=255)
                                                 tGGAGCTTTGTAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTg                                                                                            <  1:2697636/140‑1 (MQ=255)
                                                  ggAGGTTTGGAAAAAAAGCGGCGGCATTGACCTGATGAAGGACTTGAAAAAATTTCATCAATAGTCAAAATAACCCGGTGCGACTGCCGTTCGGCAGGCGCAATGGTACCACGAAAGGAGAACAGATATGGAAACAGTGc                                                                                           <  1:5313546/140‑1 (MQ=255)
                                                  gccgCTTTGCAAAAACAGCGGCGGCAATGACCGGATCCCGGACTTGAACAAATTGCATCACTCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACCGATATGGAAACAGTGc                                                                                           <  1:4489024/137‑1 (MQ=255)
                                                    aGCTTTTGAAAAAAAGCGGCGGCAATTACCTGATGAAGTAATTGAACAAATTTCATCAATCGTCAAAATAAACCGGTGCGCCTGCCTTTCTGCAGGCGCACTGTTACCACGAAAGGATAACAGATATGGAAACAGTGCCa                                                                                         <  2:4823800/140‑1 (MQ=255)
                                                    aGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTCGGCCGGCGCCCTGGTACCACGAAAGGAGAACAGGTATGGTAAAAGTGccc                                                                                         >  2:181191/1‑139 (MQ=255)
                                                    aGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTCGGCCGGCGCACTGGGACCCCGAAAGGGGAACACCTATGGAAACAGTGCCa                                                                                         >  2:166832/1‑140 (MQ=255)
                                                      cTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCCCTGGTACCACGAAAGGCGAACAGATATGGAAAGCGTGCCaaa                                                                                       >  2:767198/1‑140 (MQ=255)
                                                         tGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGGGGCGGCCGTTGGGCCCGCGCACGGGTACCACCAAAAGAGAACACAAATTGAAACAATTCCaaaaaa                                                                                    >  1:443507/1‑140 (MQ=255)
                                                         tGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGGCTTGCGGTCGGCAGGCGCCCAGGGACCACGAAAGGCGAAAAGATAAGGTAACACTGCCAaagaa                                                                                    >  1:3733600/1‑140 (MQ=255)
                                                         tGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTCGGCAGGCGCACGGGTACCACGAAAGGAGAACATATAAGGAAACAGTGCCAaagaa                                                                                    >  2:1717788/1‑140 (MQ=255)
                                                          ttaaaaaaaGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  1:1447106/138‑1 (MQ=255)
                                                          ggAACAAAAGCGGCGGCACTGACCTGATGTAGCACTTGAACAAATTGAATCAATCGTCAACATACACGGGTGCGCCTTCCGTTCGTCAGGCGCACTGGTACCTCGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  1:5351977/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGTTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  2:583611/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  2:3088911/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  2:2269853/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  1:2327717/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTAAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  1:676334/140‑1 (MQ=255)
                                                          ggAAAAAAAGCGGCGGCAATGACCTGATGAAGGAATTGAACAAATTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAaagaag                                                                                   <  1:5721610/140‑1 (MQ=255)
                                                            aaaaaaaGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAggg                                                                                 <  2:2338902/140‑1 (MQ=255)
                                                               aaaaGCGGCGGCAATGACCTGATGAAGGACTTGACCAAATTGCATCAATCGTCAAAATACCCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGGGCCAAAGAAGGgaga                                                                              <  1:982201/140‑1 (MQ=255)
                                                               aaaaGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGgaga                                                                              <  1:3140062/140‑1 (MQ=255)
                                                               aaaaGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGgaga                                                                              <  2:364125/140‑1 (MQ=255)
                                                               aaaaGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGGGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGgaga                                                                              <  1:12760/140‑1 (MQ=255)
                                                               aaaaGCGGCGGCAATGACATGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGCTATGGAAACAGTGCCAAAGAAGGgaga                                                                              <  2:2016790/140‑1 (MQ=255)
                                                                 aaGCGTCGGAAATGACCTTATGAAGGACTTGAACAAATTGCATAAATCGTCAAAATAAACCGGGGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTTCCAAAGAAGGGAGAtt                                                                            <  1:183090/140‑1 (MQ=255)
                                                                      gcggcAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAACCCGGGGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCt                                                                       <  2:6824059/140‑1 (MQ=255)
                                                                       cggcAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTg                                                                      <  1:2600670/140‑1 (MQ=255)
                                                                           aaTGACCTGATGCAGGACTTGAACAAAGTGCATCACTCGTCAAAATACCCCGGGGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTtct                                                                  <  1:6240941/140‑1 (MQ=255)
                                                                           aaTGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCACCATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGACCCGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTtct                                                                  <  1:285509/140‑1 (MQ=255)
                                                                           aaTGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTtct                                                                  <  1:5911004/140‑1 (MQ=255)
                                                                           aaTGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTtct                                                                  <  1:6509660/140‑1 (MQ=255)
                                                                           aaTGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGCTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTtct                                                                  <  1:1907268/140‑1 (MQ=255)
                                                                             tGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCa                                                                <  1:6614284/140‑1 (MQ=255)
                                                                              gACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCAc                                                               <  1:929601/140‑1 (MQ=255)
                                                                              gACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCAc                                                               <  1:3627704/140‑1 (MQ=255)
                                                                                           aCTTGAACAAATTGCATCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGc                                                  <  1:4067753/140‑1 (MQ=255)
                                                                                             ttGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCAc                                                <  1:5141448/140‑1 (MQ=255)
                                                                                              tGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCAcc                                               <  1:6324135/140‑1 (MQ=255)
                                                                                              tGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCAcc                                               <  1:4001283/140‑1 (MQ=255)
                                                                                              tGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTGGGCCGGCGCCCTGGTACCACGAAAGGAGAACAGATATGGAAACACTGCCCAATTAGGGAGCTTCACGGGTTATCACTGCTGGAAAAGGCAcc                                               >  2:1439786/1‑140 (MQ=255)
                                                                                              tGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGGTGGGCAGGCGCCCTGGTACCACGAAAGGCGAACAGATATGGAAACACTGCCAACTAAGGGGGCTTAACCTGTTGGCAATGGTGGCAAAGGCAcc                                               >  2:5953896/1‑140 (MQ=255)
                                                                                                aaCAAATTGCATCAATCGTCAAAATACACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAg                                             <  2:2069980/140‑1 (MQ=255)
                                                                                                       tGCATCAATCGTCAAAATAAACCGGTGCGCCAGCCGGTAGGCAGGCGCCCAGGTACCAACCAAGGAGAACAGATATGGAAACACTGCCCAAGAACGGAGAGTCCCCCGTTGTAACGGCGGGGCAAGGCCCCAGCTGGATg                                      >  2:411062/1‑140 (MQ=255)
                                                                                                          aTCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCCGGCGCACTGGTACCACCAAAGGAGAACAGATATGGAAACAAGGCCAAAGAAGGGAGATTCACCGGTTCTCACAGCAGGAAAAGGCCCCAGCGGGATGGcg                                   >  2:5524440/1‑139 (MQ=255)
                                                                                                          aTCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAACGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCt                                   <  2:3748568/140‑1 (MQ=255)
                                                                                                          aTCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGAGATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCt                                   <  2:2971039/140‑1 (MQ=255)
                                                                                                           tCAATCGTCAAAATAACCCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTg                                  <  2:6256199/140‑1 (MQ=255)
                                                                                                           tCAATCGTCAAAATAAATCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTg                                  <  2:7321985/140‑1 (MQ=255)
                                                                                                                   cAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAg                          <  2:7041472/140‑1 (MQ=255)
                                                                                                                   cAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAg                          <  2:6889679/140‑1 (MQ=255)
                                                                                                                            cccgTGCGCCGGCCGGTCGGCAGGCGCACTGGGACCACGAAACGAGAACAGATCTGGAAACAGTGCCAAAGCAGGGAGAGTCACCTGGTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACaaa                 <  2:5912801/137‑1 (MQ=255)
                                                                                                                            ccGGTGCGCCTGCCGTTCGGCAGGGGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACaaa                 <  1:6816181/140‑1 (MQ=255)
                                                                                                                            ccGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACaaa                 <  1:7131568/140‑1 (MQ=255)
                                                                                                                            ccGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACaaa                 <  1:3401825/140‑1 (MQ=255)
                                                                                                                            ccGGTGCGCCTGCCGTTCGGCAGGCGCACTGGGACCACGAAAGGAGAACAGAGATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACaaa                 <  1:1717788/140‑1 (MQ=255)
                                                                                                                                tGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATTGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAACTGGATGGCTGCGCGCAAGCGGGACAAATGGc             >  2:5688893/1‑140 (MQ=255)
                                                                                                                                 gcgcCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCt            <  1:3798633/140‑1 (MQ=255)
                                                                                                                                 gcgcCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCt            <  2:4427166/140‑1 (MQ=255)
                                                                                                                                        ccGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCTTTAtctt     <  1:5555402/140‑1 (MQ=255)
                                                                                                                                        ccGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAAATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCTTTAtctt     <  1:2167925/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACACAATTTATCATTGGAGAAATTGACGAAAAAGGGTTCGAGGATGCGGTGGAGCTTTGGAAAAAAAGCGGCGGCAATGACCTGATGAAGGACTTGAACAAATTGCATCAATCGTCAAAATAAACCGGTGCGCCTGCCGTTCGGCAGGCGCACTGGTACCACGAAAGGAGAACAGATATGGAAACAGTGCCAAAGAAGGGAGATTCACCTGTTCTCACTGCTGGAAAAGGCACCAGCTGGATGGCTGCGCTCAAGCGGGACAAATGGCTTTATCTTCTT  >  NZ_CP034943/762246‑762524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: