Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 803,252 G→T 7.9% S37R (AGC→AGA yfmC ← Fe(3+)‑citrate ABC transporter substrate‑binding protein YfmC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943803,2520GT 7.9% 1192.5 / 11.9 494S37R (AGC→AGAyfmCFe(3+)‑citrate ABC transporter substrate‑binding protein YfmC
Reads supporting (aligned to +/- strand):  ref base G (251/182);  new base T (59/2);  total (310/184)
Fisher's exact test for biased strand distribution p-value = 5.45e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 518 total aligned reads displayed.

TTCCCACCGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAA  >  NZ_CP034943/803119‑803391
                                                                                                                                     |                                                                                                                                           
 tCCCACCGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCgc                                                                                                                                      <  1:981469/140‑1 (MQ=255)
    cACCGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCgctgc                                                                                                                                   <  2:3057106/140‑1 (MQ=255)
       cGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGACTCCTTAGACTTGCTTTCGCTGCTTc                                                                                                                                <  1:2104055/140‑1 (MQ=255)
       cGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTc                                                                                                                                <  2:6781987/140‑1 (MQ=255)
       cGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTc                                                                                                                                <  1:159857/140‑1 (MQ=255)
       cGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTc                                                                                                                                <  2:4346132/140‑1 (MQ=255)
       cGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTc                                                                                                                                <  1:1006213/140‑1 (MQ=255)
          cGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGc                                                                                                                             >  2:7098478/1‑140 (MQ=255)
               tGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGCTCACTCTAGAATCCTTAGACTTGCTTTAGCTGCTTGCGCTGTTa                                                                                                                        >  2:6154975/1‑140 (MQ=255)
               tGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACACTAGAATCCTTAGACTTTCTTTCGCTGCTTCCGCTGTTa                                                                                                                        >  2:1356201/1‑140 (MQ=255)
                 ccGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACGTCTTCGTGCTGGATCACGCTAGAATACTTAGACTTGGTTTCGCTGCGTCCGCTGGTAtt                                                                                                                      >  2:5944935/1‑140 (MQ=255)
                    agaTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTGCTTATTGGGGGTGGTTCACGCAATAATCCTTATACTTTGTTTCGGTGCTTGCGCGGGTATTctt                                                                                                                   >  1:3617956/1‑139 (MQ=255)
                    agaTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACTCTATAATACTTAGACTTTCTTTAGCTGCTTGCGCTGTTAttatt                                                                                                                   >  2:129512/1‑137 (MQ=255)
                    agaTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTAATTCGTTCTGGGTAACACTAGAAAACATAGAAATGCTTTCGCTGCATCCGCTGTTAtaatg                                                                                                                   >  1:2748234/1‑136 (MQ=255)
                     taTTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTctgg                                                                                                                  <  2:3996021/139‑1 (MQ=255)
                       ttGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTctggct                                                                                                                <  2:7688377/140‑1 (MQ=255)
                              cAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGaa                                                                                                         <  1:830416/140‑1 (MQ=255)
                              cAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGaa                                                                                                         <  1:5772766/140‑1 (MQ=255)
                              cAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGaa                                                                                                         <  1:709973/140‑1 (MQ=255)
                               aGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGAATTGCTTTCGCTGCTTGCGCTGTTATTCTGGCTGGATGAAc                                                                                                        >  2:1753460/1‑140 (MQ=255)
                               aGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTATTGGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGGTATTCTGGCTGGATGTAc                                                                                                        >  2:6129412/1‑140 (MQ=255)
                                gCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCTTGTTGGGGGTGGATCACTCTATAATCCTTATAATTGCTTTGGCTGCTTGCGCGGTTATTATGGGTGGAttgaca                                                                                                       >  1:5209805/1‑135 (MQ=255)
                                gCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCTTCTTGGTGGTGGTTCAATCGAGAATCCTTAGACTTTCTTTCGCTGCTTCCCCTGTTATTATGGCTTGTTGaaaa                                                                                                       >  1:6584085/1‑138 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTGCTTCTTGGTGGTGGTTCACACTATAAACCTTATACATTGTTTTGCTGCTTACGCTGTTATTATGGGTGGTTGAACAg                                                                                                      >  1:1144224/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTGCTTCTTAGTGGTGGATGACTCTATAATACGTAGACTTGGTTTGGCTGCTTCCGCGGTTATTGTGGGGGGGTGAACAg                                                                                                      >  1:3996738/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTGTTCGTGCTGGATCACTCTAGAATGCTTAGACGTGCTTTCGCTGCTTCCGCTGTTATTCTTGCTGGGTGAAccg                                                                                                      >  2:2123571/1‑138 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTATGGGTGGTTGAACAg                                                                                                      >  2:1130735/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATAACTCTAGAATCCTTAGACTTGGGTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAg                                                                                                      >  2:729907/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCAGTCTTCGTGCTGGATAACTCTACAAACCTTAGACTTGCGTTGGCTGCTTCCGCTGGTAATCTCGGTGGAAGCACAg                                                                                                      >  2:5794337/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATGACTCTAGAATACTTAGACTTGCTTTGGCTGCTTCCGCGGTTTTTCTTGCGGGATGTACAg                                                                                                      >  1:7410074/1‑140 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACTCTAGAATACTTAGACTTGCTTTAGCTGCTTCCGCTGTTATTATTGCTGGATGaaaac                                                                                                      >  2:6004145/1‑137 (MQ=255)
                                 cATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCTTGTTCGTGGTGGATCACGCTAGAATACTTAGGCTTTCTTTCGCTGGTTGCGCGGTTTTTATGGGGGGGTGTACAg                                                                                                      >  1:6050976/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTGTGCGGGGTGGGGCACGCTAGTATACGTAGACTTGCTTTCGCTGCTTCCGCTGGTATTCTGGCGGGCTGTACAGCCg                                                                                                   >  1:4787752/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATACTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTTGCTTGTTGTACAGCCg                                                                                                   >  2:1883140/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTAGTGCTGGATCACTCAAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCg                                                                                                   >  2:1668961/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTGTTGGTTGTGTGTGCTGGATCACGCTAGAAACCGTTTACATGATTTGGCTGCGTACGGGGTTTTTATTGGTGGGTGTACAGCCg                                                                                                   >  1:1988717/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCGTGTTAGTGCTGGGTACCTCTAGAATCCATAGACTTGCGTTGGCTGCTTACGCTGTTATTGTGGGTGGTTGTAAAGCCg                                                                                                   >  1:4550308/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCGTCTTCGTGCTGGATCACTCTAGAATCCGTAGACTTGCTTTGGCTGCTTCCGCTGTTATTCTGGCTGGGTGAACAGCCg                                                                                                   >  2:2719759/1‑140 (MQ=255)
                                    ccAAGAATGAAAGCTCAAGGACAACCACTCGGTTAGGTGTGCCGCTTACTGTCGTGGTTAAGTCTTCGTGCTGGGGCACTGTTGAATCCTTTGCGTTGGTTTGGGGGCTTGGCCTGTTATTCTGGGTGGGTGTCCAGCCg                                                                                                   >  1:6831852/1‑140 (MQ=255)
                                      caGAATGAAAGCTCAAGGACAACCACTAGTTTAGGTGTGCCTATTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGa                                                                                                 <  2:7610123/139‑1 (MQ=255)
                                      aaGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGa                                                                                                 <  2:7523430/140‑1 (MQ=255)
                                      aaGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGa                                                                                                 <  2:4832216/140‑1 (MQ=255)
                                        gAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCGGGCTGGATGAACAGCCGGATa                                                                                               <  2:2688275/140‑1 (MQ=255)
                                         aaTGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGaa                                                                                                         <  1:5690927/129‑1 (MQ=255)
                                             aaaGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTTCTTCTTAGTGGTTGTTCACGCTAGAATCCTTTGACGTTGGTTGGCGGGGTGCGCTGTTATTGTGGGTGGGTGGAAACCCGGATaacaag                                                                                          >  1:6717310/1‑135 (MQ=255)
                                             aaaGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTGCTTGTTCGTGGTGGGTCACTCTAGAATCCTTAGACTTTGTTTCGCTGCGTCCGCTGGTATTGTGGCTGGATGAACAGGCGGATACAATg                                                                                          >  2:3204155/1‑140 (MQ=255)
                                             aaaGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCGTCTTCGTGGGGGATTACTCTAGAAACCATCGACTTTGGTTCGGAGCTTGAGGGGTTATTCTGTCTGGATGTAAACCCGGAGACaaag                                                                                          >  1:4714679/1‑138 (MQ=255)
                                             aaaGCTCAAGGACAACCACGCGTTTAGGTGTGCCGCTTACTGTCGTTTTTACTTCTTCGTGCTGGATCACTCTAGAAACCTTAGACTTTCTTTCGCTGCTTCCCCTGTTATTCTGGCTGGATGAACAGCCGGATACAATg                                                                                          >  2:62287/1‑140 (MQ=255)
                                                           aCCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTATTCGTGCTGGATCACTCTAGAATCCTTAGACTTTCTTTAGCTGCTTCCGCGGTTATTATGGCTGGATGAACACCCGGATACAAAGAAAAAGGCGATtaa                                                                            >  2:2559364/1‑140 (MQ=255)
                                                            ccACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTGCTTGTTACTGCTGGATAACACGAGAATCCGGAGACTTGCTTTGGCTGCTTACGCTGTTATTCTTGGGGGATGAAAACCCGGATACAATAAAAAAGGCGAGtaat                                                                           >  2:3676916/1‑140 (MQ=255)
                                                            ccACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCATCGTGCTGGATCAATATAGAAACCGTAGAATTTATTTAGCTGCATCCGCAGTTATTATGGCTTGATGAACACCCCGAAAAAAATAAAAACGCGATtaat                                                                           >  2:2469741/1‑140 (MQ=255)
                                                              aCTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATtaataa                                                                         <  2:1471155/140‑1 (MQ=255)
                                                               cTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATaaa                                                                        <  1:7669561/140‑1 (MQ=255)
                                                                tCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATaaaa                                                                       <  2:5224771/140‑1 (MQ=255)
                                                                tCGTTTAGGTGTGCCGCTTACTGTCGTGTTTCCTTCTTCGTGCTGGATCACTCTACAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGATGGATGAACAGCCGGATAAAATGAAACAGGCGATTAATaaaa                                                                       >  2:6956064/1‑140 (MQ=255)
                                                                  gTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAaca                                                                     <  2:3140420/140‑1 (MQ=255)
                                                                         tgtgCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGa                                                              <  2:5577331/140‑1 (MQ=255)
                                                                             ccGCTTACTGTCGTTTTTGCTTATTCGTTCTGGATCACACTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTATGGGTGGATGAACAGCCGGATACAATGAAACAGGCGCTTAATAAAAAACTCATGATatc                                                          >  2:4919463/1‑138 (MQ=255)
                                                                             ccGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAAAACTTAGAATTGCTTTCGCTGCTTACGCTGTTATTCTGGCGGGATGAAAAGCCGGATACAAAGAAAAAGGCGATTAATAAAACAATCATTATAGc                                                          >  2:5470257/1‑140 (MQ=255)
                                                                             ccGCTTACTGTCGTTTTTCCTTCATCGTGGTGGCTCACTCTAGAATCCGTAGACTTGCTTTAGCTTCTTCCGCTGTTATTCTTGCTGGATGAACAGCCCGATACAATTAAAAAGGCGATTAATAAAAAACACATGATAGc                                                          >  2:845631/1‑140 (MQ=255)
                                                                             ccGCTTACTGTCGTTTTTACTTCTTAGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTACCCGGTTATTATGGCTGGATGAACAGCCGGATACAAATAAAAACGCGATTAATAAAACACTCATGATAGc                                                          >  2:6406429/1‑140 (MQ=255)
                                                                                cTTACTGTCTTTTTTCGTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTTCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGACCAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAAt                                                       <  2:6634345/140‑1 (MQ=255)
                                                                                cTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGTTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAAt                                                       <  1:3840290/140‑1 (MQ=255)
                                                                                   aCTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCaa                                                    <  2:6316460/140‑1 (MQ=255)
                                                                                   aCTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCaa                                                    <  2:354924/140‑1 (MQ=255)
                                                                                   aCTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCaa                                                    <  1:5486620/140‑1 (MQ=255)
                                                                                   aCTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCaa                                                    <  2:5698501/140‑1 (MQ=255)
                                                                                    cTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAAc                                                   <  2:5038259/140‑1 (MQ=255)
                                                                                            tttCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGa                                           <  1:4197791/140‑1 (MQ=255)
                                                                                              tCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATCGCAATCAACTTGTTAGAAt                                         <  2:7439685/140‑1 (MQ=255)
                                                                                                       gTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCa                                <  2:2264028/140‑1 (MQ=255)
                                                                                                             gATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCAt                          >  1:732463/1‑140 (MQ=255)
                                                                                                             gATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCAt                          >  1:5067607/1‑140 (MQ=255)
                                                                                                                  ctctAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCAttcttc                     >  2:7019706/1‑140 (MQ=255)
                                                                                                                  ctctAGAATCCTTAGACTTGCTTTCGCTGCTTCAGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGAAATCAACTTGTTAGACTAGGTGCGCATGTCAttcttc                     >  2:804073/1‑140 (MQ=255)
                                                                                                                   tctAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTcc                    >  2:2023364/1‑140 (MQ=255)
                                                                                                                   tctAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTcc                    >  2:3345859/1‑140 (MQ=255)
                                                                                                                            cTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAACACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAg           <  1:614880/140‑1 (MQ=255)
                                                                                                                            cTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAg           >  1:1812101/1‑140 (MQ=255)
                                                                                                                            cTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAg           >  1:4334342/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:5381980/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  2:951679/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:1553547/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:2205384/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:318664/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:3308714/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:3474962/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  2:5342371/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:632025/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:7003729/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  2:415861/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  2:4707182/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCAGTCTTCCTTTTTTCAGa          >  1:2894345/1‑140 (MQ=255)
                                                                                                                             ttAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACCTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGa          >  1:5854607/1‑140 (MQ=255)
                                                                                                                               aGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGAtt        >  2:3445041/1‑140 (MQ=255)
                                                                                                                               aGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGAtt        >  2:4533623/1‑140 (MQ=255)
                                                                                                                               aGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGAtt        <  1:1468900/140‑1 (MQ=255)
                                                                                                                                 aCTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTa      >  1:3762755/1‑140 (MQ=255)
                                                                                                                                  cTTGTTTTCGCTGCTTCTGCTGTTATTCTGGCTGGATGAAGAGACGGATACTATGAATCAGGCTATTATTAAAACACTCTTGATATAAATCAACTTTTTAGAATAGTTGCTCATGTGATTCTTCATTTTTTCAGATTTAg     <  2:650914/140‑1 (MQ=255)
                                                                                                                                     gCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTaa  >  2:3833460/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                           
TTCCCACCGGCGTAATGCCGAGATTGTATACAGCATCCAAGAATGAAAGCTCAAGGACAACCACTCGTTTAGGTGTGCCGCTTACTGTCGTTTTTCCTTCTTCGTGCTGGATCACTCTAGAATCCTTAGACTTGCTTTCGCTGCTTCCGCTGTTATTCTGGCTGGATGAACAGCCGGATACAATGAAACAGGCGATTAATAAAACACTCATGATAGCAATCAACTTGTTAGAATAGGTGCGCATGTCATTCTTCCTTTTTTCAGATTTAGTAA  >  NZ_CP034943/803119‑803391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: