Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 896,727 C→A 5.6% H99N (CAT→AAT)  recX → recombination regulator RecX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943896,7270CA 5.6% 918.3 / 3.9 352H99N (CAT→AAT) recXrecombination regulator RecX
Reads supporting (aligned to +/- strand):  ref base C (166/151);  new base A (2/33);  total (168/184)
Fisher's exact test for biased strand distribution p-value = 3.21e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GATTCAATACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATCGACGACGATAC  >  NZ_CP034943/896588‑896863
                                                                                                                                           |                                                                                                                                        
gaTTCAATACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATcc                                                                                                                                          <  1:1989513/140‑1 (MQ=255)
    cAATACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATaa                                                                                                                                      >  2:870994/1‑140 (MQ=255)
    cAATACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATaa                                                                                                                                      >  2:6073671/1‑140 (MQ=255)
     aaTCCGGCGATGAAGTAAAGAAAGCCTTCAACCGTGCGCTTGAAATTTTATCCTACCGGATGCGGTCAACGAAAGCGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCAGTGCGGAAGTCATCCATAAg                                                                                                                                     <  1:871131/140‑1 (MQ=255)
     aaTACGGCGCTGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAg                                                                                                                                     <  2:4132414/140‑1 (MQ=255)
     aaTACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGACAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAg                                                                                                                                     <  2:6903844/140‑1 (MQ=255)
     aaTACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAg                                                                                                                                     >  1:989501/1‑140 (MQ=255)
     aaTACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAg                                                                                                                                     <  1:610319/140‑1 (MQ=255)
     aaTACGGCGATGAAGTAAAGAAAGGCTTAAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAg                                                                                                                                     <  1:5882994/140‑1 (MQ=255)
        aCGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTg                                                                                                                                  <  2:2342574/140‑1 (MQ=255)
         cGGCGATGAACTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTGAGCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGa                                                                                                                                 <  1:3970793/140‑1 (MQ=255)
          ggCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGaa                                                                                                                                <  2:1728807/140‑1 (MQ=255)
           gCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAAc                                                                                                                               <  1:5069590/140‑1 (MQ=255)
            cGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAATAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACg                                                                                                                              <  2:4461769/140‑1 (MQ=255)
            cGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACg                                                                                                                              <  2:6860616/140‑1 (MQ=255)
            cGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACg                                                                                                                              <  1:803280/140‑1 (MQ=255)
              aTGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGACGTCATCCATAAGCTGAACGAc                                                                                                                            >  2:303826/1‑140 (MQ=255)
               tGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACt                                                                                                                           >  2:2374874/1‑140 (MQ=255)
               tGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACt                                                                                                                           >  2:3574054/1‑140 (MQ=255)
               tGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAACCTGAACGACt                                                                                                                           >  2:6067385/1‑140 (MQ=255)
                 aaGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTAc                                                                                                                         <  1:2416412/140‑1 (MQ=255)
                      aagaaagGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTa                                                                                                                    >  2:5724058/1‑140 (MQ=255)
                           agGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTaa                                                                                                               >  1:6383582/1‑140 (MQ=255)
                           agGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTaa                                                                                                               >  1:6306294/1‑140 (MQ=255)
                            ggCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATATGCTGAACGACTACAAGTACTTaaa                                                                                                              >  1:543903/1‑140 (MQ=255)
                            ggCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTaaa                                                                                                              >  1:6600208/1‑140 (MQ=255)
                                       tGCTCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAt                                                                                                   >  2:6240288/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAACGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGCCCAGGAAt                                                                                                   >  2:1631955/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGACGTCATCCATAAGCTGAACGACTCCAAGTACTTAAACGACCAGGact                                                                                                   >  2:5308730/1‑138 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCCTAAGCTGAACGACTACAAGTACTTAAACGACCAGGact                                                                                                   >  2:4029519/1‑138 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACCAGTACTTAAACGACCAGGAAt                                                                                                   >  2:6311140/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTCCTTAAACGACCAGGAAt                                                                                                   >  2:2643469/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAt                                                                                                   >  2:4825814/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAt                                                                                                   >  1:6450884/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAt                                                                                                   >  2:5909807/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAt                                                                                                   >  1:2148258/1‑140 (MQ=255)
                                       tGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGGTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCAGTGCGGAAGTCATCCATAAGCTGAACGACTACGAGTACTTCCACGACCGGGagt                                                                                                   >  1:5408943/1‑138 (MQ=255)
                                       tGCGCTTGAATGTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGCCCACTTAATGAAACAAGAAATTTCGGCGCCCGTCAGTGCGGAAGTCAGACATAAGCTGACCGACTACCAGTAGGTAAACGAcgcggact                                                                                                   >  1:4085418/1‑133 (MQ=255)
                                         cgcTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAACTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCCGGAAttt                                                                                                 >  2:6520976/1‑140 (MQ=255)
                                             ttattttttATCCTACCGTATGAGGTCAACTAAAGAGGTTGTGGACCACTTAAAGAAAAAATAAATTGCGGCGCCCGGAATTGCGGAAGTCATCAATACGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  1:3330986/136‑1 (MQ=255)
                                             ttaaTTTTTATCCTACCGTTTGAGGTCAACGAAAGAGGTTGAGGACCAATTAAATAAAAAAAATTTTTCGGCGCCCGTCTTTGCGGAAGTCATCCATAAGCTTAACGAATAAAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  1:7675136/138‑1 (MQ=255)
                                             ttaaTTTTTATCCTACCGTATTAGGAAAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  2:2977038/138‑1 (MQ=255)
                                             ttaaTTTTTATCCTACCGTATGAGGTAAACGAAAGCGTTTGAGTACCACTTAAATAAAAAACAATTTTCGGCGCCCGTCTTTGCGGAAGTCATCCATAAGATGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  1:3418111/138‑1 (MQ=255)
                                             tGATTTTTTATCCTACCGGATGAGGACAACTAAAGCGGTTGAGCACCACTTAAAGAAAAAAGAATTTTCGGCGCCCGTCATTGCGGACGTCATCCATACGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  1:3005049/140‑1 (MQ=255)
                                             tGAATTTTTATCCTACGGGTTGAGTTAAACTAAAGTGGTTGAGCACCAATTAAAGAAAAAACAATTTTCGGGGCCCGTATTTGCGGAAGTCATCCATAAGCTTAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  1:559710/140‑1 (MQ=255)
                                             tGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  2:5061583/140‑1 (MQ=255)
                                             ggAATTTCTACCCTACCGGATGCGGTCACCCAAAGAGGATCAGCACCACTCAAACCAAAAAAAAAATTCGGCGCCCGTCATTGCGGAAGTAATCAATACCCTGAACGACTACAAGTACTTCAACGACCAGGAATTTGCcg                                                                                             <  1:6816051/139‑1 (MQ=255)
                                               aaTTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcgcg                                                                                           >  1:1004039/1‑140 (MQ=255)
                                                  ttttATCCTCCCGCAGGCGGACCCCGAACGAGGTGGAGGACCACTAAACGAAAAAACAACTGTCGGCCCCCGTCATTGGGGAAGTCATCCATCAGCGGAACGACTACACTTACTTAAACGACCAGGAATTTGCCGCGGcc                                                                                        <  1:2599633/140‑1 (MQ=255)
                                                  ttttATCCTACCGGATGAGGTCAACTAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAGGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGcc                                                                                        <  2:678137/140‑1 (MQ=255)
                                                  ttttATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGACAAAACAAATTTCGGCGCCCGTCATTGCGGACGTCATCCATAAGCGGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGcc                                                                                        <  1:5338000/140‑1 (MQ=255)
                                                    ttATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTa                                                                                      <  2:7533149/140‑1 (MQ=255)
                                                                     gTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             >  1:1755242/1‑116 (MQ=255)
                                                                     gTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCcg                                                                                             <  2:1755242/116‑1 (MQ=255)
                                                                     gTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACaaaa                                                                     >  1:2916859/1‑140 (MQ=255)
                                                                      tcgaCGAAAGAGGTCGAGGATCACTTAAAGAAAAAAGAAACCTCGCCCCCCGTCATTGCGGAAGTCATCCATAGGCTTAACGATTACAAGTACTTAAACGACCAGGAATTTGCCGCAGCCTATGTCAGCACTCACaaaaa                                                                    >  1:4762362/4‑140 (MQ=255)
                                                                      tCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACaaaaa                                                                    >  2:3359064/1‑140 (MQ=255)
                                                                           gAAAGAGTTTGTGTACAAATTAAATAAAAAAGAAATTTCGGCGCCCTTCATTGCGTAATTCATCAATAAGCTGAACGAATACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAaacga                                                               <  1:58386/140‑1 (MQ=255)
                                                                              agagGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAaacgaacg                                                            >  1:117453/1‑140 (MQ=255)
                                                                                agGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGa                                                          >  2:7056179/1‑140 (MQ=255)
                                                                                  gTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGaaa                                                        >  2:7421751/1‑140 (MQ=255)
                                                                                    tGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATACGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAACAg                                                      >  1:3525730/1‑140 (MQ=255)
                                                                                    tGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAAGTTGCCGCGGCCTATGTCAGCTCCCACAAAAAAACGAACGGAAAAg                                                      >  1:7508486/1‑140 (MQ=255)
                                                                                     gAGGACCCCTTAAAGAAAAAATAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGATGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAgg                                                     <  2:1127721/140‑1 (MQ=255)
                                                                                          ccACTTAAACAAAAAAGAAATTTCGGCGCCCGTCTTTGCGTAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTg                                                <  2:4638013/140‑1 (MQ=255)
                                                                                          ccACTTAAACAAAAAACAAATTTCGGCGCCCGTCATTGCGGAAGACACCAATACGCTCAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTg                                                <  1:1072220/140‑1 (MQ=255)
                                                                                            aCTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGAc                                              >  2:1330611/1‑140 (MQ=255)
                                                                                             cTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACg                                                                                                                              >  2:3747199/1‑59 (MQ=255)
                                                                                             cTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACg                                             >  1:1198023/1‑140 (MQ=255)
                                                                                             cTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACg                                             >  1:3762943/1‑140 (MQ=255)
                                                                                             cTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACg                                             >  2:4876005/1‑140 (MQ=255)
                                                                                               aaaacaaaaaacaaaTTGCGGCGCCCGACATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGtt                                           <  1:6168598/139‑1 (MQ=255)
                                                                                                 aagaaaaaacaaaTTGCGGCGCCCGTCATTGCGGAAGTCATCAATACGCTCACCGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGtttt                                         <  1:3987274/140‑1 (MQ=255)
                                                                                                   gaaaaaagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGt                                       >  2:5457826/1‑140 (MQ=255)
                                                                                                     aaaaagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTc                                     >  1:2798438/1‑140 (MQ=255)
                                                                                                      aaaagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCa                                    >  2:5515129/1‑140 (MQ=255)
                                                                                                       aaagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCag                                   >  1:5022968/1‑140 (MQ=255)
                                                                                                        aagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAACGGCCCTGACGTTTTGTTCaga                                  <  2:3832811/140‑1 (MQ=255)
                                                                                                        aagaaaTTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGttt                                          >  1:2368310/1‑132 (MQ=255)
                                                                                                              tttCGGCGCCCGTCTTTGCGGAAGACATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTg                            <  1:6981699/140‑1 (MQ=255)
                                                                                                                    cgcCCTTCTTTGGGTACGTAAACCATAAGCTGAACTACTACAAGTACTTAAACGACCACGAATTTGCCGCGGCCTAGGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCa                      <  1:138981/140‑1 (MQ=255)
                                                                                                                        cGTCATTGCGGAAGTCATCCATAGGCTTAACGATTACAAGTACTTAAACGACCAGGAATTTGCCGCAGCCTATGTCAGCACTCACAAAAAAACAAACGGAAAAGGCCCTGATGTTTTGTTCAGAGAGCTAAGAGCGAAgg                  <  2:4762362/140‑1 (MQ=255)
                                                                                                                                cGGAAGTCATCCATAACCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGACCGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgac          <  2:503044/140‑1 (MQ=255)
                                                                                                                                cGGAAGTAATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAACGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgac          <  2:3289707/140‑1 (MQ=255)
                                                                                                                                  gAAGTCATCCATACGCTGAACGACTACAAGTAATTAAACGACCAGGATTTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacga        <  2:5826882/140‑1 (MQ=255)
                                                                                                                                  gAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacga        <  2:5265179/140‑1 (MQ=255)
                                                                                                                                  gAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacga        <  2:2121801/140‑1 (MQ=255)
                                                                                                                                  gAAGTCATCAATACGATGAACGACTAAAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacga        <  1:5243072/140‑1 (MQ=255)
                                                                                                                                   aaGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgac       >  1:1608121/1‑140 (MQ=255)
                                                                                                                                     ttcatcaATACGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  2:714036/139‑1 (MQ=255)
                                                                                                                                     ttaaTCCATAAGCTCAACGACTACAATTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:4825814/137‑1 (MQ=255)
                                                                                                                                     ttCATCCATACGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  2:2888796/139‑1 (MQ=255)
                                                                                                                                     tacATCAATAAGCTGAACGACTAAAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:3076617/138‑1 (MQ=255)
                                                                                                                                     gtaaTCCATACTCTCAACGACTACAATTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCCCAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:728240/137‑1 (MQ=255)
                                                                                                                                     gtaaTCAATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:5714737/137‑1 (MQ=255)
                                                                                                                                     gTCATCCATAAGCTGAACGACTACACGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAACGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:6725528/140‑1 (MQ=255)
                                                                                                                                     gTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  2:5128490/140‑1 (MQ=255)
                                                                                                                                     gTCACCAATAAGCTTAACCAATACAAGTACTTAACCGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGACCGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATcgacgacga     <  1:2576643/140‑1 (MQ=255)
                                                                                                                                        ttCCATAAGCTGAACGACTACATGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATCGACGACGATAc  <  2:6443953/139‑1 (MQ=255)
                                                                                                                                        aTCCATAAGCTGAACGACTACAATTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATCGACGACGATAc  >  1:2966666/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
GATTCAATACGGCGATGAAGTAAAGAAAGGCTTCAACCGTGCGCTTGAATTTTTATCCTACCGGATGAGGTCAACGAAAGAGGTTGAGGACCACTTAAAGAAAAAAGAAATTTCGGCGCCCGTCATTGCGGAAGTCATCCATAAGCTGAACGACTACAAGTACTTAAACGACCAGGAATTTGCCGCGGCCTATGTCAGCACCCACAAAAAAACGAACGGAAAAGGCCCTGACGTTTTGTTCAGAGAGCTGAGAGCAAAGGGAATCGACGACGATAC  >  NZ_CP034943/896588‑896863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: