Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 986,801 G→T 12.0% D372Y (GAT→TAT)  spoVR → stage V sporulation protein SpoVR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943986,8010GT12.0% 925.4 / 22.2 408D372Y (GAT→TAT) spoVRstage V sporulation protein SpoVR
Reads supporting (aligned to +/- strand):  ref base G (174/161);  new base T (2/66);  total (176/232)
Fisher's exact test for biased strand distribution p-value = 2.51e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 415 total aligned reads displayed.

GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGTA  >  NZ_CP034943/986664‑986940
                                                                                                                                         |                                                                                                                                           
gCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGAt                                                                                                                                           >  2:7517323/1‑140 (MQ=255)
          tGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATTTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcg                                                                                                                                 >  1:3734201/1‑140 (MQ=255)
           ggCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAAtcgt                                                                                                                                >  1:1341631/1‑140 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           <  2:2744864/140‑1 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           >  2:3986171/1‑140 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           >  2:4983699/1‑140 (MQ=255)
                aggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           >  2:6128593/1‑140 (MQ=255)
                aggatgggATAAAATGTTTTAGGTATGGGAAATTGAATCCGATATCTCTTTCATCCGAAATTACTTAACAAAAGAATTAGTTATGCGCGTGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACa                                                                                                                           <  1:4380357/135‑1 (MQ=255)
                 ggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaa                                                                                                                          >  1:491121/1‑140 (MQ=255)
                 ggcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaa                                                                                                                          >  2:5949484/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAACACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:4169708/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         <  1:101460/140‑1 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:6365165/1‑140 (MQ=255)
                  gcaggGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACaaa                                                                                                                         >  1:5866048/1‑140 (MQ=255)
                      ggAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAAt                                                                                                                     >  1:6227065/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                               >  1:7482443/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                               >  2:6759164/1‑140 (MQ=255)
                            aaTGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAAt                                                                                                               >  2:2579613/1‑140 (MQ=255)
                                       tCAGGGCAATTGCAGCCGATATCTCTTTCCTCCGCAAGTACTTACCAACAGACTTAGTTAGGCGCGAGGATCTTTATTTGTGTCAACAACAGGGCAGAGATTATAAAATCGTCGACAAATAATGGAAATCGGTACGAGAc                                                                                                    <  2:1372281/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:1548151/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:5393805/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:6583188/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:5838313/140‑1 (MQ=255)
                                       tCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAc                                                                                                    <  2:5695778/140‑1 (MQ=255)
                                        cAGGTAAATTGAAGCCGATATCTCTTTCATACGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGAcc                                                                                                   <  2:6407520/140‑1 (MQ=255)
                                          gggAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAg                                                                                                 <  2:2066429/140‑1 (MQ=255)
                                               aTTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGt                                                                                            <  1:1814530/140‑1 (MQ=255)
                                                ttGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTg                                                                                           >  1:3397687/1‑140 (MQ=255)
                                                    ctCCGATATCTCGTTCATCCGCAATTACTTAACAAAAGACGTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGACTGGAAATCGGGACGCGACCAGCTCGTGAGCa                                                                                       <  2:693364/139‑1 (MQ=255)
                                                    aTCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                                       <  1:1404102/140‑1 (MQ=255)
                                                      ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                                     <  2:6258728/140‑1 (MQ=255)
                                                      ccGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATg                                                                                     <  1:4784465/140‑1 (MQ=255)
                                                          tatCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCggg                                                                                 <  2:3195711/140‑1 (MQ=255)
                                                             ctctTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACCAAGAATGGAAATAGGTACGCGACCAGCTCGGGAGCGTGCGGGTaa                                                                              >  2:6358690/1‑140 (MQ=255)
                                                               ctTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCCGATATTATAAAATCGTCGACACAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAc                                                                            >  1:3103511/1‑140 (MQ=255)
                                                               ctTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGGACTCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAc                                                                            >  2:1897819/1‑140 (MQ=255)
                                                               ctTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAc                                                                            >  2:6513744/1‑140 (MQ=255)
                                                                  tCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAcggc                                                                         <  2:3232013/140‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                   <  1:3025516/140‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                   <  2:3799875/140‑1 (MQ=255)
                                                                        gCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGttt                                                                   <  1:5440128/140‑1 (MQ=255)
                                                                         cAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTc                                                                  >  2:4742002/1‑140 (MQ=255)
                                                                          aaTTACATAACAAAACACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTcc                                                                 <  2:2301034/140‑1 (MQ=255)
                                                                               cTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATAtt                                                            <  1:535002/140‑1 (MQ=255)
                                                                                         gACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAATAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACAGCGGGTTTCCATATTTGACAGTaaa                                                  >  1:7552875/1‑140 (MQ=255)
                                                                                         gACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGACATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTcaa                                                  >  2:3346561/1‑140 (MQ=255)
                                                                                         gACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTaaa                                                  >  2:1222050/1‑140 (MQ=255)
                                                                                          aCTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAac                                                 >  2:5217491/1‑140 (MQ=255)
                                                                                            ttttttttGCGCTAGTATATTTTTTTGTTAAAAAAAATGGGCAGTTTTTATAAAATCTTCGAAAAAGAATGTAAATCGGTCCGCGACCAGATCGTGAGCTTGCGGGTAACCGGCGGGTTTCCATATTTGACAGTACacga                                               <  1:7662571/133‑1 (MQ=255)
                                                                                              aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGt                                                                     >  1:1207866/1‑116 (MQ=255)
                                                                                              aGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAacgacg                                             >  1:97054/1‑140 (MQ=255)
                                                                                                    gcgcGAGTATATTTATTTTTTAAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGAtt                                       <  2:4489393/140‑1 (MQ=255)
                                                                                                    gcgcGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCa                                                                                       <  1:3776716/92‑1 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                      >  1:6478342/1‑140 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                      >  2:2238532/1‑140 (MQ=255)
                                                                                                     cgcgAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTa                                      >  1:7194677/1‑140 (MQ=255)
                                                                                                         aGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACTACGGTGATTACATg                                  >  2:479640/1‑140 (MQ=255)
                                                                                                                  tttttttTAAAAAACTGGGCTTATATTATAAAATCGTCTAAAAAGATTGGAATTGGGTACGCGACCAGCTCGTTAGCATGGGGGTAAACGGGGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  1:5107742/139‑1 (MQ=255)
                                                                                                                  tttttttTAAAAAACTGGGCAGATATTATAAAATGGTCGAAAAAGATTGTAAAGCGTTAGGCGACCAGCTCGTGCGATTGCGGGTAACCGGGGGTTTTCCATATTTGACTGTACACGACGGTGATTACATGAAAAATAAc                         <  1:1123438/139‑1 (MQ=255)
                                                                                                                  ttttGTTTCAAAAACTGGGCAGAGTTTATAAAATCGTCGACAAAGAATGTAAATCGGTACGCGACCAGCTCGTGAGAATGCGGGTAAACGGCGGGTTTCCATTTTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:357198/139‑1 (MQ=255)
                                                                                                                  ttttGTTACAAAAACTGGGAATATTTTATAAAATCGTCGACAAATAATGGAAATCGGTACGCGACCAGCTGGTGAGCATGCGGTTAAACGGGGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  1:7380043/139‑1 (MQ=255)
                                                                                                                  aTTTTTTTAAAAACCAGGGCAGAGATTATAAAATCGTCGAAAAATAATGGAAATCGGTACGCCACCAGCTCGTGAGAATGCGGGTAAAGGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  1:6106311/140‑1 (MQ=255)
                                                                                                                  aTTTTTATCAAAAACAGGTCAGACATTATAAATTCGACGACAAAGAATGTAATTGGGTACGCCAACAGATCGTTAGAATGCGTGTAAACGGAGGGTTTCAATATTTCACCTTAAACGACGGTGATTACATGAAAAATAAc                         <  1:3539741/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  2:7265655/140‑1 (MQ=255)
                                                                                                                  aTTTGTTTAAAAAACAGGGCCGATATTTTAAAATCTTCCACAAACAATGTAAATGGGTCCGCTCCAATCTGGTGAGCTGGGGGGTAAAGGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAAc                         <  1:4983699/140‑1 (MQ=255)
                                                                                                                      ttttCAAAAACAGGGCTGATATTATAAAATCGTCGACAAATATTGTAAATGGGTACGCGACCAGCTCGTGAGAATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     <  2:765803/139‑1 (MQ=255)
                                                                                                                      ttttCAAAAAATGGGACGATTTTATAAAATCTTCTACAAACAAGGTAATTCGTTCCGCCACAAGCTCTTGTGCAGGGGGTCAAACGGCGGGTTTCAATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     <  1:4528068/139‑1 (MQ=255)
                                                                                                                      ttttCAAAAAAAGGGCATAGATTATAAAATCTTCGACAAATAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     <  2:4296623/139‑1 (MQ=255)
                                                                                                                      ttttAAAAACCAGGCCCCAGATTATAAAATCGTCCACAAAGACTGCAAATCGGTACGCCACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTCACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     <  2:2914366/139‑1 (MQ=255)
                                                                                                                      gTTTAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAATATTGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGc                     <  2:1128619/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGTCAGATATTATAAATTCTTCTAAAAAAAATGTAAATCGGTACGCTACCAGCTCGTTAGCATGCGGTTAAACGCCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:903195/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCATATATTATAAAATCTTCGACAAAGAATGTAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACATTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:2354153/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGATATTATAAAATCGTCGACAAATAATGTAAATCGGTACGCGACCAGCTCGTGAGAATGCGGTTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:2156213/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGATATTATAAAATCGTCGACAAAGATTGTAAATCGGTACGCGACCAGCTCGTGAGCTGGGGGGTAAACGGCGGGTTTCATTATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:7020748/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:4169708/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGCAGAGATTATAAAATCGTCGAAAAAGATTGGAAATGGGTACGCGACCAGCTCGTGAGCATGGGGGTAAACGGCGGGTTTCCATATTTTAAAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:5468141/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGGAAGAGATTATAAAATCGTCGACAAAGACTGTAAATGGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGTGTTTCCATATTTGACAGGAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:3641820/140‑1 (MQ=255)
                                                                                                                       tttCAAAAACAGGCCAGATATTATAAAATCGTCGACAAAGATTGTAATTCGGTCCGCCACAAGCTCGTGCGCAGGCGGGTAACCGGCGGGTTTCCATATTTGACAGTACACGACGGTGATTACATGAAAAATAACGAGct                    <  1:6635142/140‑1 (MQ=255)
                                                                                                                       tttAAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATGGGTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:7403813/140‑1 (MQ=255)
                                                                                                                       ttaaaaaaaaCGGGCATATATTATAAAACCGCCGACAAATATTGCAAATCGGTACGCGACCAGCTGGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:2276338/136‑1 (MQ=255)
                                                                                                                       ttaaaaaaaCTGGGCAGTCTTTATAAAATCGTCGAAAAATATTGCAAATCGGTACGCGACCAGCTCGTGAGATTGCGGGTAACCGGCGGGTTTCAATATTTGACAGTACACGACGGTGATTACATGAAAAATAACGAGct                    <  1:2480558/136‑1 (MQ=255)
                                                                                                                       ttaaaaaaaCAGGGCAGATATTATAAAATGGTCCAAAAATATTGTAAATGGGTACGCGACCAGATGTTGCGCATGGGGTTAAACGGCGGGTTTCATTTTTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:2805726/136‑1 (MQ=255)
                                                                                                                       tgtCAAAAACCGGGCATCGATTATAAAATCGTCGACAACGAATGTAAATCGTTCCCCGACCAGCTGGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGACAAATAACGAGct                    <  2:954312/138‑1 (MQ=255)
                                                                                                                       taacAAAAACAGGCCAGCTATTATAAAACCGACCACAAATAATGCAATACGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  1:3937676/137‑1 (MQ=255)
                                                                                                                       gtccAAAACCCGGGCAGAGATTCAACAAGCGTCGACCAAGACTGGAAATGGGTACGCGCCCAGCTCGTGTGAATGCGGGTAAACGTCGGGTTTCCATATTTGACTGTAAACGACGGTGATTACATGAAAAATAACGAGct                    <  2:6882329/137‑1 (MQ=255)
                                                                                                                        ttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAACTAACGAGctc                   >  1:6428726/1‑140 (MQ=255)
                                                                                                                        ttCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGCGTTTCCATATTTGACAGTAAACGACGGTGATTAAATGAAAAATAACGAGctc                   >  1:6757773/1‑140 (MQ=255)
                                                                                                                         tCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGctct                  >  2:3092849/1‑140 (MQ=255)
                                                                                                                            cgacaggggAAGATATTATAAAATCGTCCACAAATAATGGAAATCGTTACGCGACCAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCtata               <  2:162029/134‑1 (MQ=255)
                                                                                                                              aaCTGGGCAGCTATTATAAAATCGTCTAAAAAGAATGCAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  1:5949484/140‑1 (MQ=255)
                                                                                                                              aaCCGGGCAGATATTATAAAATCTTCGACAAAGAATGTAAATCGGTACGCGACCAGATCGTGAGCATGCGGGTAAACGGGGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  1:1904779/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCAGATATTATAAAATCGTCTACAAAGATTGGAAATCGGTAGGCGAACAGCTCGTGAGCATGCGGTTAAACGGCGGGTTTCCATATTTTACAGTAAACGACGTTGATTACATTAAAAATAACGAGCTCTATAtt             <  2:5088720/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCAGAGATTATAAAATCGTCGACAAATAATGTAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGTGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  2:456587/140‑1 (MQ=255)
                                                                                                                              aaCAGGGCAGAGATTATAAAATCGTCGACAAAGAATGCAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAAGGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATAtt             <  2:491121/140‑1 (MQ=255)
                                                                                                                               actgggCAGAGTTTATAAAATCGTCCACAAACACTGGAACTGGTTAGGCGACACGCTGGTGCGCTTGCGGGTAACGGGGGGTTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTa            <  1:158056/137‑1 (MQ=255)
                                                                                                                               aCATGGAAGTGATTATAAACTCGTCGACAAAGAAGGGAAATCGGTACGCGACCTGATCGTCAGCATCCGGGTAAAGCCCGGGTTTCCATATTTGACTGTACCCGACGGTGATTACATCAAAAATAACGAGCTCTATATTa            <  1:5538467/140‑1 (MQ=255)
                                                                                                                                ctgggCAGATATTATAAAATCGTCCACAAATAATGCAAATGGGTAGGCGACCAGCTGGTGAGCATGGGGTTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTaa           <  1:152262/138‑1 (MQ=255)
                                                                                                                                   ggCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa        >  2:6206632/1‑140 (MQ=255)
                                                                                                                                   gccAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCa        <  2:7095751/138‑1 (MQ=255)
                                                                                                                                    gCAGATTTTATAAAATCGTCGACAAAGAATGGAAAGGGGTACGCGACCAGCTGGTGAGCTTGCGGGTAAACGGCGGGTTTCAATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCAt       <  1:7323536/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
GCGTGAAGCCTGGCTCAGGCAGGGAGAAAATGTTTGAGGTCAGGGAAATTGAATCCGATATCTCTTTCATCCGCAATTACTTAACAAAAGACTTAGTTATGCGCGAGGATCTTTATTTGTTTCAAAAACAGGGCAGAGATTATAAAATCGTCGACAAAGAATGGAAATCGGTACGCGACCAGCTCGTGAGCATGCGGGTAAACGGCGGGTTTCCATATTTGACAGTAAACGACGGTGATTACATGAAAAATAACGAGCTCTATATTAAGCATTGGTA  >  NZ_CP034943/986664‑986940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: