Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,014,046 G→T 10.1% A44E (GCA→GAA)  EO946_RS05230 ← polysaccharide deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,014,0460GT10.1% 1057.8 / 23.8 439A44E (GCA→GAA) EO946_RS05230polysaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base G (154/225);  new base T (58/2);  total (212/227)
Fisher's exact test for biased strand distribution p-value = 2.85e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 440 total aligned reads displayed.

TTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACAATC  >  NZ_CP034943/1013909‑1014183
                                                                                                                                         |                                                                                                                                         
   aGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTc                                                                                                                                      >  2:394509/1‑140 (MQ=255)
   aGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTc                                                                                                                                      >  2:3397484/1‑140 (MQ=255)
   aGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTGTGGTGGCACTGACTTCCTTTGCgtta                                                                                                                                      >  1:4303929/1‑139 (MQ=255)
     tAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGa                                                                                                                                    <  2:1615869/140‑1 (MQ=255)
     tAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGa                                                                                                                                    <  2:7540453/140‑1 (MQ=255)
      aaaCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGAt                                                                                                                                   <  1:3094584/140‑1 (MQ=255)
       aaCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATg                                                                                                                                  <  2:2459517/140‑1 (MQ=255)
        aCCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCGTCAGGCTTTCCTTCTCATTGCGAAGGTGGCCGTTTGTGGTGGCAGTTTCGTCCTTTGGTTTCGGTGa                                                                                                                                 >  1:5622082/1‑140 (MQ=255)
         ccGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCTTCTCAATGCGAAGCTGGCCGGGTTTGGTGGCACTGACTTACATTGCTTTCGATTAg                                                                                                                                >  1:919603/1‑140 (MQ=255)
          cGTCTTATCTCCCATGTTCTGCTTTGTCTGGAGATGTTTTCTCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                                                               <  1:383872/140‑1 (MQ=255)
          cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                                                               <  1:3718268/140‑1 (MQ=255)
          cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                                                               <  2:556184/140‑1 (MQ=255)
          cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGc                                                                                                                               <  1:2078796/140‑1 (MQ=255)
          cGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGAGGAGc                                                                                                                               <  1:5378056/140‑1 (MQ=255)
            tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTg                                                                                                                             <  2:5777873/140‑1 (MQ=255)
            tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTg                                                                                                                             <  1:7571292/140‑1 (MQ=255)
            tCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCGTCAGGCTTTCCTTCTCATTGCGAAGCTGGGCGTTTGTGGTGGCAGTGAATTCCTTTTCTTTCGATGAGcgg                                                                                                                             >  1:2131088/1‑138 (MQ=255)
             cTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGt                                                                                                                            >  2:3803223/1‑140 (MQ=255)
             cTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGt                                                                                                                            <  2:3537079/140‑1 (MQ=255)
               tATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTg                                                                                                                          >  2:5038848/1‑140 (MQ=255)
               tATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTg                                                                                                                          <  2:4500043/140‑1 (MQ=255)
               tATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTg                                                                                                                          <  2:6735467/140‑1 (MQ=255)
                 tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGa                                                                                                                        <  1:7085008/140‑1 (MQ=255)
                 tctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTAAATTCGAAGCTTGCCCGTTTTGGTGGCACTGAAATACATTGCTTTAGAATAGCTGTTTGa                                                                                                                        >  1:1293845/1‑140 (MQ=255)
                  ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                                                       <  1:7303917/140‑1 (MQ=255)
                  ctcCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                                                       <  1:2410010/140‑1 (MQ=255)
                  ctcCCATGTTCTGCTTTGTCTGCAGATGATTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGaa                                                                                                                       <  1:5060564/140‑1 (MQ=255)
                      cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTAAGGCTTTCCGTCGCATTTGGAAGCGGGCCGTTTTTGGTGGCCGTGACTTACTGTTGTTTGGATGGGCGGTTGGAATaac                                                                                                                   >  1:6801514/1‑138 (MQ=255)
                      cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCATCTTAAGGCGTTACTTGTCATTTCGAAGCTTGCCGGTTGTGGTGGCACTGACTTACTTTGAGTTCGATGAGGTGTTGGacttcc                                                                                                                   >  1:4930623/1‑135 (MQ=255)
                      cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATAcc                                                                                                                   >  2:4735223/1‑140 (MQ=255)
                      cATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTATCATTTCGAAGCTGGCCGTTTTTTGTGGCAGTGACTTACATTGCTTTCGATGATCAGTTTGaaaaac                                                                                                                   >  1:7004608/1‑136 (MQ=255)
                            cTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTGTGGTGGCAGTGACTTCCTTTGCTTTCGGTGAGCTGTTGGAATACCCGAGaa                                                                                                             >  2:2123156/1‑140 (MQ=255)
                            cTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCGAGaa                                                                                                             >  2:6988291/1‑140 (MQ=255)
                            cTGCTTTGTCTGCACATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCATCAGGCTTTAATACTCATTGCCAAGCTGGCCGGTTGTGGTGGCAGTGAATTCCTTTGGTTTCGATGAGGGGTTTGTATACCCTAGaa                                                                                                             >  2:537269/1‑140 (MQ=255)
                             tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                                                            >  2:484382/1‑140 (MQ=255)
                             tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                                                            >  2:3266829/1‑140 (MQ=255)
                             tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                                                            <  1:347756/140‑1 (MQ=255)
                             tGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCGAGaaa                                                                                                            >  2:5321829/1‑139 (MQ=255)
                                  tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCa                                                                                                       <  2:635209/140‑1 (MQ=255)
                                  tGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCa                                                                                                       <  1:6079821/140‑1 (MQ=255)
                                    tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTGCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTCTTTCGTTGTGCTGTTGGAATAACCTAGAATCACCATa                                                                                                     >  1:7623130/1‑140 (MQ=255)
                                    tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCATTGCTTTAGCTGAGCTGTTGGAATACCCTAGAATCACCaaa                                                                                                     >  2:100977/1‑138 (MQ=255)
                                    tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAACTGGCCGTTTGTGGTGGCAGTGACATCCATTTCTTTCGATGAGGTGTTGGAAAACCCTAGAATCACCaaa                                                                                                     >  2:6517785/1‑138 (MQ=255)
                                    tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCGTTGCTTCTCATTTCGAAGCTGGGCGTTTGTTGTGGGCGTGAATTGCTTTTGGTTGGATGGGGTGTTGGGATAACCTAGAATCACCaaa                                                                                                     >  1:4048038/1‑138 (MQ=255)
                                    tCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTGCTTATCATTTGGAATCTGGCCGTTTTTGGTGGCAGTGAGTTCCATTTGTTTCGATTAGGTGTTGGAATAACCTGGAATAACCaaa                                                                                                     >  1:1457005/1‑138 (MQ=255)
                                            tGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAag                                                                                             <  1:6423846/140‑1 (MQ=255)
                                            tGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAag                                                                                             <  1:2502666/140‑1 (MQ=255)
                                              ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTAGATTTGCTGTTGGAATACCCGAGAATGACCATAAGCAAAagca                                                                                           >  2:607016/1‑140 (MQ=255)
                                              ttttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCGTTCCGTCTCATTTCGAAGCAGGCCGTTTGTGGTGGCACTGACTTGCTTTTGTTTCGAGGAGCGGTTGGAATACCCTAGAAGAAACAaaaccaaaacca                                                                                           >  1:5843399/1‑140 (MQ=255)
                                                ttATCAGCAGCTCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGCTGGCAGTGACTTCCTTTGCTTGCGATGAGCTGTTGGAATACCCTAGAAGCACCATAAGCAAAagcagc                                                                                         <  1:5686446/140‑1 (MQ=255)
                                                ttATCAGCAGATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAAGACCCGAGAATCACCATAAGCAAAagcagc                                                                                         <  2:1483788/140‑1 (MQ=255)
                                                ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                                                         <  2:3617033/140‑1 (MQ=255)
                                                ttATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAagcagc                                                                                         <  1:118300/140‑1 (MQ=255)
                                                      gcagATCGGGGGAGTCGTTTTTGAGCTTCAGGCTTTCCGTCTCATTGCGAAGCTGGCCGGTTGTGGTGGGCGTGACTTCCTTTGGGTTCGATTTTCTGTTTGCATAACCGAGAATCACCAAAACCAAAAACAGcacacaa                                                                                   >  1:4975393/1‑139 (MQ=255)
                                                       cagATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGGGGTGGCAGTGACTTACTTTTCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAACAGCACACATa                                                                                  >  2:2003297/1‑140 (MQ=255)
                                                       cagATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTACTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTCTTTCGATGAGCTGTTTGAATAACCTATAATCACCATAAGCAAAATCAGCACACATa                                                                                  >  2:4081501/1‑140 (MQ=255)
                                                       cagATCGGGGGAGTCGTTTTTCAGCTTCAGGCGTTCCTTCTCATTGCGAAGCTGGCCGTTTGGGGTGGCAGTGACTTCCTTTTCTTTCGATGAGCTGTTTGAATACCCTAGAAAAACCATAAGCAAAAGCAGcacacaaa                                                                                  >  2:143834/1‑138 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  1:1742304/140‑1 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  1:4702574/140‑1 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  2:3479683/140‑1 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  2:2312361/140‑1 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  1:6286202/140‑1 (MQ=255)
                                                        agATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTGTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaa                                                                                 <  1:3198993/140‑1 (MQ=255)
                                                          aTCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTACTTTGCTTTCGATGAGCTGTTTGAATAACCGAGAATCACCATAACCAAAAGCAGCACACATaaaa                                                                               >  2:7441620/1‑140 (MQ=255)
                                                          aTCGGGGGAGTCGTTTTTAAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTTCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATaaaa                                                                               >  2:1296316/1‑140 (MQ=255)
                                                             gggggAGTCGTTTTTAAGCTTCATGCTTTACTTCTCATTTCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTAGATTAGCTGTTGGAATACCATAGAATAACCATAAGCAAAATCAGCACACATAAAAATg                                                                            >  2:2206359/1‑140 (MQ=255)
                                                                    tCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACt                                                                     >  2:1814563/1‑140 (MQ=255)
                                                                    tCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGGTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACt                                                                     >  2:7257644/1‑140 (MQ=255)
                                                                    tCGTTTTTCAGCTTCAGGCTTTCCGTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCATTGCTTTCGATTAGCTGTTTGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACAAAAAACAGCTAAAcg                                                                     >  2:2777181/1‑139 (MQ=255)
                                                                                         tCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATAcc                                                                                                                   <  1:2803288/73‑1 (MQ=255)
                                                                                         tCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGa                                                >  1:2665557/1‑140 (MQ=255)
                                                                                         tCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCAACATAAGCAAAAGCAGCACACATAAAACTTCAAAACTTGCAAATATAAAATCAAGCGa                                                >  1:4197180/1‑140 (MQ=255)
                                                                                         tCCTTCTCAGTGCGAAGGTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGa                                                <  1:394509/140‑1 (MQ=255)
                                                                                          ccTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGaa                                               <  1:1814563/140‑1 (MQ=255)
                                                                                          ccTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGaa                                               <  1:4430303/140‑1 (MQ=255)
                                                                                              ctcATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAATCGAAAAgg                                           >  2:4386875/1‑140 (MQ=255)
                                                                                                 aTTGCGAAGCGGGCCGTTTGTGGGGGCAGTGACTTCCTTTTCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAg                                        <  1:2268647/140‑1 (MQ=255)
                                                                                                  ttGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGa                                       <  2:4545227/140‑1 (MQ=255)
                                                                                                   tGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGaa                                      >  1:5498922/1‑140 (MQ=255)
                                                                                                   tGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGaa                                      >  2:6140960/1‑140 (MQ=255)
                                                                                                   tGCGAAGCTGGCCGTTTGTGGTGGCAGTGACGTCCGTTGCTTTCGATGCGCTGGTGGCATACCCGCGTATCTCCATAAGCAAAAGCAGCACACCTAAAACTGCTAAACTTGCAAATTTAAAAGCACGCGGAATGGCATaa                                      >  1:4079755/1‑140 (MQ=255)
                                                                                                      gAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGga                                   <  2:3065767/140‑1 (MQ=255)
                                                                                                           tGGCCGTTTGTGGTGGCAGTGTCTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGc                              <  2:7144477/140‑1 (MQ=255)
                                                                                                           tGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGaacc                              >  2:4962683/1‑138 (MQ=255)
                                                                                                           tGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGc                              <  1:4531705/140‑1 (MQ=255)
                                                                                                             gCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGt                            >  1:6725679/1‑140 (MQ=255)
                                                                                                             gCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGt                            >  2:1693803/1‑140 (MQ=255)
                                                                                                              ccGTTTGTGGTGGCAGTGACTTCCTGTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGtc                           <  2:6663810/140‑1 (MQ=255)
                                                                                                                  ttGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAAt                                                                                                            <  1:1000677/55‑1 (MQ=255)
                                                                                                                       gtggCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCttgt                  >  2:7359351/1‑140 (MQ=255)
                                                                                                                          gCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCttgtttg               <  2:4196248/140‑1 (MQ=255)
                                                                                                                          gCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCttgtttg               <  2:5942076/140‑1 (MQ=255)
                                                                                                                             gTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACAAAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCg            <  2:1242671/140‑1 (MQ=255)
                                                                                                                                aattCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGTAGAAGCGTCTCCTCTTGTTTGTCGCAt         <  1:1630206/138‑1 (MQ=255)
                                                                                                                                aCTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCAt         <  2:847720/140‑1 (MQ=255)
                                                                                                                                aCTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCAt         <  2:1187161/140‑1 (MQ=255)
                                                                                                                                    ccTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACa     >  1:1497570/1‑140 (MQ=255)
                                                                                                                                     cTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACaa    <  2:2556355/140‑1 (MQ=255)
                                                                                                                                      tttGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACAAt   <  1:7476359/140‑1 (MQ=255)
                                                                                                                                       ttGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACAAtc  <  1:6551454/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                         
TTAAGTAAACCGTCTTATCTCCCATGTTCTGCTTTGTCTGCAGATGTTTTATCAGCAGATCGGGGGAGTCGTTTTTCAGCTTCAGGCTTTCCTTCTCATTGCGAAGCTGGCCGTTTGTGGTGGCAGTGACTTCCTTTGCTTTCGATGAGCTGTTGGAATACCCTAGAATCACCATAAGCAAAAGCAGCACACATAAAACTGCTAAACTTGCAAATTTAAAAGCAAGCGAAATGGCAGAAGGAGAAGCGTCTCCTCTTGTTTGTCGCATAACAATC  >  NZ_CP034943/1013909‑1014183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: