Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,097,451 C→A 8.7% P65Q (CCA→CAA)  EO946_RS05675 → MarR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,097,4510CA 8.7% 725.6 / 13.5 290P65Q (CCA→CAA) EO946_RS05675MarR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (154/100);  new base A (2/34);  total (156/134)
Fisher's exact test for biased strand distribution p-value = 9.79e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

TCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTG  >  NZ_CP034943/1097314‑1097590
                                                                                                                                         |                                                                                                                                           
  cgcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                                                                         >  2:761207/2‑140 (MQ=255)
  agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACACTGAACcaatc                                                                                                                                         >  2:7154314/1‑140 (MQ=255)
  agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                                                                         >  1:1163668/1‑140 (MQ=255)
  agcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatc                                                                                                                                         >  1:3697917/1‑140 (MQ=255)
   gcagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatca                                                                                                                                        <  1:3188071/140‑1 (MQ=255)
    cagGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaa                                                                                                                                       >  2:2395450/1‑140 (MQ=255)
     agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGCGCACGGGAGTAAATCTCAATTATCGATGACAGAAAATCATGTTCTTTCTGGTGTTGGGGACAATGAACcaatcact                                                                                                                                      >  2:33250/1‑138 (MQ=255)
     agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                                                                      >  2:7346057/1‑140 (MQ=255)
     agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                                                                      >  1:4461439/1‑140 (MQ=255)
     agGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACcaatcaat                                                                                                                                      >  1:7102363/1‑140 (MQ=255)
       gagaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGt                                                                                                                                    <  2:2086865/140‑1 (MQ=255)
        agaAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTc                                                                                                                                   <  1:4542959/140‑1 (MQ=255)
              gggAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCACTCAATGTCACGTCt                                                                                                                             >  2:1737964/1‑140 (MQ=255)
                 aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                          >  1:65701/1‑140 (MQ=255)
                 aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                          >  2:7374642/1‑140 (MQ=255)
                 aGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCGTGTGGTGGGGACAATGAACCAATCAATGTCACGTCTAtt                                                                                                                          >  1:42833/1‑140 (MQ=255)
                  ggggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATTAACCAATCAATGTCACGTCTATTg                                                                                                                         >  2:3232453/1‑140 (MQ=255)
                  ggggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTg                                                                                                                         >  2:1492992/1‑140 (MQ=255)
                    ggCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCa                                                                                                                       >  2:5089915/1‑140 (MQ=255)
                        cATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTg                                                                                                                         <  2:5956547/134‑1 (MQ=255)
                              aGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGa                                                                                                             >  1:905994/1‑140 (MQ=255)
                               gCCTTTTTGGCTGACGAATTGAAGAAGCTCGGTGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                            <  2:4662104/140‑1 (MQ=255)
                               gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCTATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                            <  1:7038032/140‑1 (MQ=255)
                               gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                            >  2:7666177/1‑140 (MQ=255)
                               gCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGaa                                                                                                            <  1:6964877/140‑1 (MQ=255)
                                 cTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATa                                                                                                          <  1:5125040/140‑1 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGaa                                                                                                                    >  1:7156269/1‑128 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATAct                                                                                                        >  1:4085530/1‑139 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:5408769/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:4877515/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:4760829/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:6342851/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:2182076/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:4657899/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  2:6440450/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:3945845/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAAGACg                                                                                                        >  1:6241834/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCGATTGCAGAAAAAATGAATACg                                                                                                        >  2:5219359/1‑140 (MQ=255)
                                   ttttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTAATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACg                                                                                                        >  1:1236553/1‑140 (MQ=255)
                                     tttggtGAGGAATTTAAGATGTTCTTTTTCGTGCAGGGGATTAAATCTCAATTATCTATGACATAATTTAATGTTCTTTCTTGTTTTGGGTACAATTAACCAATCAATTTAACGTCTATTGCAGAAAAAATGAATACGAc                                                                                                      <  2:306651/135‑1 (MQ=255)
                                     ttGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGAc                                                                                                      <  2:3448642/140‑1 (MQ=255)
                                        gCTGACTATTTCAAGACTCTCGTTGTCGACCACGGCATAAAACCGAAATTATCTTTCAAACAAATACATGTTCTTTGTTTTTTTGGGGACATTTAACAAATAATTGTAAGGTCTATTTCACAAAAAATGAATACGACaaa                                                                                                   <  2:226637/140‑1 (MQ=255)
                                          tGACGAATTGATCAAGCTCTATGTCGAGCAGGGGATTAAATCTAAATTATCGATGACAGAAATTAATTTTCTTTCTTGTTTTGGGGAAAATCAACCAATCAATGTCACGTTTATTGCACAAAAAATTATTACGAAAAAAg                                                                                                 <  2:3902738/140‑1 (MQ=255)
                                            aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                               >  1:2470339/1‑140 (MQ=255)
                                            aCGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCg                                                                                               >  1:1962064/1‑140 (MQ=255)
                                              gAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATTAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGAc                                                                                             >  2:5825627/1‑140 (MQ=255)
                                              gAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAATAATGAATACGACAAAAGCGAc                                                                                             >  2:1401954/1‑140 (MQ=255)
                                                     acaagCTCGCTGTCGAGCAGGGGATTAAATCTCAATTATCGAGGACAGAAATTATTGTTCTTTCTGGTTTTGGGGAAAATTAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACCAAAAACGCGACTGTATcg                                                                                      <  2:2695878/138‑1 (MQ=255)
                                                        agCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCgcgc                                                                                   >  1:2271558/1‑140 (MQ=255)
                                                          cTCGTTGTCGAGCACGGCATTAAATCTAAATTATCGCTGACAGAAATTCATGTTGTTTGTTGTTTTGGGCACAATAAACAAAACATTGTAACTTCTATTGCAGAAAAAATTAAAACCACAAAAGCGACTGTATCGCGCAt                                                                                 <  2:4855732/140‑1 (MQ=255)
                                                          cTCGATGTCGAGCACGGTTTTAAATAACAATTATCGATGACACAAATTCATGTTCTTTGTTTTTTTGGGGAAAATGAACCAATCATGTTCACGTCTTTTGCATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCAt                                                                                 <  2:5260697/140‑1 (MQ=255)
                                                          cTCGATGTCGAGCACGGGATTAAATCTAAATTATCGATCACACAAATTCTTGTTTTTTCTTGTGTTGGGGACAATGAACCATTAAATTTCACGTCTATTGCACAAAAAATCAATACGACAAAAGCGACTGTATCGCGCAt                                                                                 <  2:2546125/140‑1 (MQ=255)
                                                             gttttCGAGCAGGGTATTAAATCTAATTTATCGATTACATAAATTAATTTTGTTTTTTTTGTTGGGGAAAATGAACAAATAATTGTCACTTATATTGAACAAAAAATCAAACCCACAAACGCGACTGTATCGCGCATCAg                                                                              <  1:6440450/136‑1 (MQ=255)
                                                              aTGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCGATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGc                                                                             >  1:1406904/1‑140 (MQ=255)
                                                              aTGTCGAGCACGGGAGTAAATCTCAATTAAAGATGACAGAAATGCATGTTCTTTCTGGTGTTGGGGACAATTAACAAATCAATGTCACCGCTATTGCAGAACAAATGAATCCGACAAAAGAGACTGTCTCGCGCCTCAGc                                                                             >  1:5934946/1‑140 (MQ=255)
                                                               tGTCGAGCACGGTAGTAAATCTAAATTATCGATGACAGAAATAAATTTTTTTTCTTGTTTTGGGCAAAATGAACCAATAATTGTCACGTATATTCCAGAAAAAATGAATACCACAAACGCGACTGTATCGCGCATCAGca                                                                            <  2:5548868/140‑1 (MQ=255)
                                                                    aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                       >  1:5957766/1‑140 (MQ=255)
                                                                    aGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAAAACGACAAAAGCGACTGTATCGCGCATCAGCACaaaa                                                                       >  2:5668895/1‑140 (MQ=255)
                                                                               aaaTCAAAATTAGCGATGAAATAAATACATGTTCTTTGTTGTGTTGGGCAAAATCAACCAAGAATTGCAACGTATATGCCACAAAAAATCAACCCCCCAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGgcgc                                                            <  1:2812890/140‑1 (MQ=255)
                                                                               aaaTATAAATTATCTATCACACAAATTCATTTTCTTTTTTGTGTTGGGCAAAATTAACAAAACAATTTAACTTATATTGCACAAAAAATTAATCCGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGgcgc                                                            <  1:7384628/140‑1 (MQ=255)
                                                                                aaTCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGcgcg                                                           >  2:3692995/1‑140 (MQ=255)
                                                                                 aTCTCATTTATGGATGACATAAATTCATGTTCTTTCTTGTGTTGGGCACAATCAACAAATCATTGTAACGTATTTTGAACAAAAAATTAAAACCCCAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCgg                                                          <  1:7666177/140‑1 (MQ=255)
                                                                                   ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                        >  1:5749018/1‑140 (MQ=255)
                                                                                   ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                        >  2:2140156/1‑140 (MQ=255)
                                                                                   ctcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                        >  1:1898375/1‑140 (MQ=255)
                                                                                   atcAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTGGGGCACAATCAACCAATCAATGTCACGTCTATTGAATAAAAAATCAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGt                                                        <  2:4589412/139‑1 (MQ=255)
                                                                                     cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                      >  2:4020103/1‑140 (MQ=255)
                                                                                     cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                      >  1:1083916/1‑140 (MQ=255)
                                                                                     cAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGttt                                                      >  2:6488340/1‑140 (MQ=255)
                                                                                      aaTTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTc                                                     <  2:3948162/140‑1 (MQ=255)
                                                                                        ttATCTATTACACAATTTCTTTTTTTTTGTTGTGTTGGGCAAAATCAACAAATCAATTTCACGTTTTTTGAATAAAATTTGATTACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGAGCGTGTTTCCt                                                   <  2:1236553/140‑1 (MQ=255)
                                                                                        ttATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCATTGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCt                                                   <  2:6181187/140‑1 (MQ=255)
                                                                                           tgtctGACTGAATTTCATGTTCTTTGTGGTGTTGGGCAAAATTAACAAATAAATTTAAGGTCTATTGCAGAAAAAATGATTACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  2:6794863/136‑1 (MQ=255)
                                                                                           tctaTGACAGAAATTCAGGTTGTTTCTTTTTTTGGGCACAATGACCAAATAAATGTCCCGTCTATGGCACAAAAAATGAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  1:2841718/137‑1 (MQ=255)
                                                                                           tctaTGAAAGAAATTAATGTTCTTTTTTGTGTTGGGGAAATTTAACAAATAATTGTCAGGTCTATGGCAGAAAAAATGAAAACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  1:1646579/137‑1 (MQ=255)
                                                                                           tcgttcaCATAAATTATTTTTTTTTGTTGTGTTGGGGAAAATGAACCAATCATTGTCACGTCTATTGCAGAAAAATTGAATACGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  1:7479676/134‑1 (MQ=255)
                                                                                           tatttGACATAAATTCTTGTTCTTTCTTGTGTTGGGGAAAATGAACCAATAATTGTCACGTATTTTGCAAAAAAAATGAATCCGCAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  1:262475/136‑1 (MQ=255)
                                                                                           tCGATGACATATATTCATTTTCTTTCTTGTGTTGGGGACATTGAAACAATCTTTGTCACGTATTTTTCATAAAAATTGAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  2:6915513/140‑1 (MQ=255)
                                                                                           tCGATGACATAAATTCTTGTTCTTTGTTGTGTTGGGCACAATGAACAAATCAATGTCACTTATATTGCAGAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  2:65701/140‑1 (MQ=255)
                                                                                           tCGATGACATAAATTCTTGTTCTTTCTTGTGTTGGGGAAAATTAACAAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTca                                                <  2:3789168/140‑1 (MQ=255)
                                                                                            cGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcac                                               >  2:2855463/1‑140 (MQ=255)
                                                                                            cGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcac                                               >  2:4759087/1‑140 (MQ=255)
                                                                                             gttGACATAAATGCATGTTCGTCCTTGTGTTGGTCACAATGACCCAATCAATGTCAGTTATATTGCTGAAAAATTGATTACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcact                                              <  2:4522212/138‑2 (MQ=255)
                                                                                             gATGACATAAATTCATTTTCTTTCTTGTTTTGGGGACAATGAACAAATCATTGTCACGTCTATTCCACAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcaca                                              <  2:7102363/140‑1 (MQ=255)
                                                                                             gATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTcaca                                              >  2:839228/1‑140 (MQ=255)
                                                                                                     aaaTTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCCCAAAATTGCGCGGCGCGGGTTTCCTTCACAGAACACAg                                      >  1:3778981/1‑140 (MQ=255)
                                                                                                     aaaTTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAg                                      >  1:3541222/1‑140 (MQ=255)
                                                                                                             gTTCTTTGTTGTGTGGGGCACAATCAACAAAGCAATGTCACTTATTTTCAAGAAAAAATCAATACCCCAACAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGa                              <  2:6331340/140‑1 (MQ=255)
                                                                                                                        ttttGGGTACAATCAACCAATCAATGTCACTTATATTGCAGAAAAAATTAATACGAAAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                   <  2:2191978/138‑1 (MQ=255)
                                                                                                                        ttttGGGCAAAATGAACCAATCACTGTCACGTCTTTTGAATAAAAAATTAATAGGACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                   <  2:557403/138‑1 (MQ=255)
                                                                                                                        tgtTGGGGAAAATTAACAAATAATTGTCACGTATATTGCATAAAAAATGAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAagg                   <  1:297786/140‑1 (MQ=255)
                                                                                                                             gggACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACCACAAACGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc              <  1:7374642/140‑1 (MQ=255)
                                                                                                                             ggcaaaaTCAACAAATCAATTTCCCGTCTATGGCAAAAAAAATCAATACCACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTc              <  1:6858755/135‑1 (MQ=255)
                                                                                                                               gACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTa            >  2:5037681/1‑140 (MQ=255)
                                                                                                                               gACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTa            >  2:4227750/1‑140 (MQ=255)
                                                                                                                               gACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTa            >  1:6095638/1‑140 (MQ=255)
                                                                                                                                  aaTGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACtt         >  1:5048547/1‑140 (MQ=255)
                                                                                                                                    tGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACCAAAAGGAGGTCTACTTcc       >  2:1583455/1‑140 (MQ=255)
                                                                                                                                    tGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGAGAACAAAAAGGAGGTCTACTTcg       >  1:5843000/1‑139 (MQ=255)
                                                                                                                                       aCCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGAt    >  2:3669531/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TCAGCAGGAGAAATGGGAGGGGCTCATTAAAGCCTTTTTGGCTGACGAATTGAAGAAGCTCGATGTCGAGCACGGGAGTAAATCTCAATTATCGATGACAGAAATTCATGTTCTTTCTTGTGTTGGGGACAATGAACCAATCAATGTCACGTCTATTGCAGAAAAAATGAATACGACAAAAGCGACTGTATCGCGCATCAGCACAAAATTGCTCGGCGCGGGTTTCCTTCACAGAACACAGCTCAGCGATAACAAAAAGGAGGTCTACTTCCGATTG  >  NZ_CP034943/1097314‑1097590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: