Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,632 T→G 10.0% L576R (CTT→CGT)  addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6320TG10.0% 959.6 / 20.6 392L576R (CTT→CGT) addBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base T (90/244);  new base G (56/2);  total (146/246)
Fisher's exact test for biased strand distribution p-value = 2.63e-25
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

AGAATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGG  >  NZ_CP034943/1111493‑1111771
                                                                                                                                           |                                                                                                                                           
aGAATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCt                                                                                                                                             <  2:1168176/140‑1 (MQ=255)
 gAATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCtt                                                                                                                                            >  2:6792638/1‑140 (MQ=255)
  aaTCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTa                                                                                                                                           >  2:4846958/1‑140 (MQ=255)
    tCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACa                                                                                                                                         <  2:721827/140‑1 (MQ=255)
      aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                       <  2:957580/140‑1 (MQ=255)
      aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                       <  1:1390806/140‑1 (MQ=255)
      aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                       <  2:5975920/140‑1 (MQ=255)
      aTTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAtt                                                                                                                                       <  1:635088/140‑1 (MQ=255)
       ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAttt                                                                                                                                      >  1:6715712/1‑140 (MQ=255)
       ttGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCAttt                                                                                                                                      >  2:3007874/1‑140 (MQ=255)
            gcgcAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTgtct                                                                                                                                 >  2:5245980/1‑140 (MQ=255)
            gcgcAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTgtct                                                                                                                                 >  2:7182991/1‑140 (MQ=255)
               cagcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAt                                                                                                                              >  2:1831999/1‑140 (MQ=255)
               cagcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTGTGTAAt                                                                                                                              >  1:5175188/1‑140 (MQ=255)
                agcagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAtt                                                                                                                             <  2:3804903/140‑1 (MQ=255)
                  cagcaTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGAGGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTGTAATTcc                                                                                                                           >  1:4231015/1‑140 (MQ=255)
                     cagcagcagCCGTGGTATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGAGGGGGGATGATGAGATTTATCTTGATTTTTTTCAGCAAATGATGGAAGCAGGCTCGGAGTAGATCACATTTTCTCTAATTCCGcc                                                                                                                        <  1:3202153/137‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  1:3643911/140‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  1:7303962/140‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  1:6792638/140‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  1:2074695/140‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  2:6712551/140‑1 (MQ=255)
                     caTCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGcc                                                                                                                        <  2:4032275/140‑1 (MQ=255)
                          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTGTCTAATTACGCCAgcgc                                                                                                                   >  1:7626884/1‑140 (MQ=255)
                          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                   <  1:5812893/140‑1 (MQ=255)
                          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                   <  2:6489877/140‑1 (MQ=255)
                          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTgcgc                                                                                                                   <  1:34660/140‑1 (MQ=255)
                          gcagGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTAAATTTGTCTAATTACACCGgcgc                                                                                                                   >  1:7043131/1‑140 (MQ=255)
                              gCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAACTTTTAGGTAATTCCGCCTTCGCtttg                                                                                                               >  2:3698566/1‑138 (MQ=255)
                               cgggggATGCAGACATTCAGCTACTTGAGGAGTTAGATGAAATGATGGGGGATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACCTTTTGTCGAAATCCGCCAGCGCCTGAc                                                                                                              >  2:7380925/4‑140 (MQ=255)
                               cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAGTCCGCCTGCGCTTGAc                                                                                                              <  2:5651358/140‑1 (MQ=255)
                               cGTGGGATGCAGTCATTCAGCTACTTGAGGAGTGTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTGTCGAATTCCGCCTtcgcgttgc                                                                                                              >  2:2015389/1‑135 (MQ=255)
                                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTGTCTAATAACCCCTGCGCGTGAcc                                                                                                             >  2:614881/1‑140 (MQ=255)
                                gTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTCTCTAATTACGCCTGCGCTTGAcc                                                                                                             >  2:7306638/1‑140 (MQ=255)
                                   ggATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAgg                                                                                                          <  2:5013808/140‑1 (MQ=255)
                                        cAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTGAATTTATGTAATTAACCCAGCGCGTGTCCAGGGGttt                                                                                                     >  1:110773/1‑140 (MQ=255)
                                          gTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGt                                                                                                   <  2:2662226/140‑1 (MQ=255)
                                           tCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTc                                                                                                  <  2:188086/140‑1 (MQ=255)
                                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                <  1:2392583/140‑1 (MQ=255)
                                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                <  2:354180/140‑1 (MQ=255)
                                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                <  1:4655237/140‑1 (MQ=255)
                                             aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCAGTTTCTCTAATTACGCCAGCGCGTTTCCAGGTGTGTGTggg                                                                                                >  2:3961245/1‑140 (MQ=255)
                                              ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAACAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTATCTAAATAACCCGGCGCATGACCACGTGtttttgggg                                                                                               >  2:3360204/1‑134 (MQ=255)
                                               tCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTGTCTAATTACGCCGGCGCTTGTCCAGGGGTTTGTGGGcc                                                                                              >  2:171866/1‑139 (MQ=255)
                                                cAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTATGTAATTACGCCTGCGCTTTTCAAGGTTTTTTTTGGCaa                                                                                             >  2:1020524/1‑140 (MQ=255)
                                                  gCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                           <  2:372385/140‑1 (MQ=255)
                                                   cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTGCAATTTTTATAAATACCCCAGCCCGTGACCAGGGGGTTGTGGGCacaaa                                                                                          >  1:937716/1‑136 (MQ=255)
                                                   cTACTGGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTATCTAAATCCGCCGGCGCTTGACCCGGTGTTTGTCGGCActat                                                                                          >  2:7433514/1‑140 (MQ=255)
                                                      cTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCAGTTTTTGTACTTGCGCCTGCGCGTGAACAGGGGTTTGTGGGCAATATTGa                                                                                       >  2:5840093/1‑140 (MQ=255)
                                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                      <  2:1571114/140‑1 (MQ=255)
                                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                      <  2:5475090/140‑1 (MQ=255)
                                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                      <  2:6384061/140‑1 (MQ=255)
                                                       ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                      <  2:2457909/140‑1 (MQ=255)
                                                        tGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAtt                                                                                     <  1:1584062/140‑1 (MQ=255)
                                                           ggagTTTGTTGAAATGATGGGGGATGAGGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTAt                                                                                  <  2:4991483/140‑1 (MQ=255)
                                                            gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                 <  1:6174805/140‑1 (MQ=255)
                                                            gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                 <  2:4191173/140‑1 (MQ=255)
                                                               tttGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTATGTAATTAAGCCTGAGCTTGTCCCAGTGTTTGTGGGGAACATTGATTTATACaa                                                                              >  1:2714423/1‑139 (MQ=255)
                                                                ttgttgAAATGATGGGTGATGTTGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGATGGCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGGGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                             <  2:839718/140‑1 (MQ=255)
                                                                ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                             <  1:384273/140‑1 (MQ=255)
                                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTATATAATTACGCCAGCGCTTGTCCACGTTTTTTTCGGGAATATGGATTTATAAAGaa                                                                            >  1:4454031/1‑140 (MQ=255)
                                                                 tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGGAGAATTTGTATAATTAAGCCAGCGCTTGACCACGGGGTTTTGGGCCAAATGGATTTATCAAGaa                                                                            >  1:1346642/1‑140 (MQ=255)
                                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACCTTTTCTCTAAATACGCCAGCGCTTGACAAGGTGTTTGTGGGCAAGATGGCTTTATACATAATTTACGGc                                                                    >  2:2157725/1‑140 (MQ=255)
                                                                         tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:4058469/140‑1 (MQ=255)
                                                                         agaccggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                    <  1:5525071/135‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  2:29050/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  2:1531905/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  2:4703641/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  2:2359942/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  2:2063316/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGAGGAGTCGCGTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  1:2852777/140‑1 (MQ=255)
                                                                          gatgGGGGATGATGAGATTTCTCTTGATGTGTTTCAGCAAATGAAGGAAGCCGGCGCGGAGTCGCTTACCTTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                   <  1:4808370/140‑1 (MQ=255)
                                                                             gggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTAAATTTCTCTAATTACGCCGGCGGTTTTACAGGTGTTTTTGGGCAATAAGGATTTATCCACAAAGTTCGGcccca                                                                >  2:7024787/1‑139 (MQ=255)
                                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  1:4972801/140‑1 (MQ=255)
                                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  1:6590137/140‑1 (MQ=255)
                                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  1:3105086/140‑1 (MQ=255)
                                                                                 gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  2:6227167/140‑1 (MQ=255)
                                                                                 gatgatGAGATTTCTCCTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                            <  1:7147897/140‑1 (MQ=255)
                                                                                     atgaGATTTCTCTTGATTTGTTTCAGCACATGATGGAAGCCGGCGCGGAGTCGCTTTAATTTTGTCTAATTCCCCCAGCGGGTGGTCCGGTGGTTGTGGGGACTATTGGTTTTTGCAGAAAGTTAGGCACCGCTTGCAcg                                                        >  2:515783/1‑139 (MQ=255)
                                                                                       gagaTTTCTCTTGATTTGTTTCAGCAAATGATGGCAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGGAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCtt                                                      <  2:141092/140‑1 (MQ=255)
                                                                                        agaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCttt                                                     <  2:3160907/140‑1 (MQ=255)
                                                                                            ttCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGc                                                 <  1:5494269/140‑1 (MQ=255)
                                                                                              ctctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTGTAAATCCGCCTGCGCTTGACCCCGTGTTTGTGGGCCAAATTGATTTATCCAGAATGTAACGCACCTCTTGCACCATTGTGcag                                               >  2:4353574/1‑138 (MQ=255)
                                                                                               tctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAATTACCCCAGCGCTTGACCAGGTGTTTGTGGGCAATATGGATTTATCCAGAATGTACGGCACCGCGTGCACCTTTGTGCggg                                              >  2:7524059/1‑140 (MQ=255)
                                                                                                    aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCa                                         <  2:4176556/140‑1 (MQ=255)
                                                                                                      ttGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGGGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCaaa                                       <  2:159883/140‑1 (MQ=255)
                                                                                                          ttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAacgac                                   <  1:5397154/140‑1 (MQ=255)
                                                                                                              gCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCg                               <  2:110773/140‑1 (MQ=255)
                                                                                                               cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                              <  2:5414964/140‑1 (MQ=255)
                                                                                                               cAAATGAGGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGGGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                              <  2:4524782/140‑1 (MQ=255)
                                                                                                                  atgatgGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAATCCGCCTGCGCGTGACCAGGTGTTTTTCGGCAATATGGATTTATACAGAATGTAAGGCACCGCTTGGACCGTTGTGCGGGGGGCAAACGACGGCGTTct                           >  2:5563161/1‑140 (MQ=255)
                                                                                                                        gAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                     <  2:3601572/140‑1 (MQ=255)
                                                                                                                        gAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                     <  2:3455836/140‑1 (MQ=255)
                                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:1019858/140‑1 (MQ=255)
                                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:1831999/140‑1 (MQ=255)
                                                                                                                         aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                    <  1:7386099/140‑1 (MQ=255)
                                                                                                                             cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                <  1:6065507/140‑1 (MQ=255)
                                                                                                                             cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                <  1:7531695/140‑1 (MQ=255)
                                                                                                                                 gcgGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCtga            <  2:7652543/140‑1 (MQ=255)
                                                                                                                                  gggAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCtgat           <  2:4622491/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGAATCATTGAAGCGCAGCAGCATCAGCAGGCGTGGGATGCAGTCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGG  >  NZ_CP034943/1111493‑1111771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: