Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,675 C→G 8.9% V590V (GTC→GTG addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6750CG 8.9% 1132.7 / 10.6 382V590V (GTC→GTGaddBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base C (98/233);  new base G (41/2);  total (147/235)
Fisher's exact test for biased strand distribution p-value = 1.48e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 390 total aligned reads displayed.

TCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTG  >  NZ_CP034943/1111536‑1111811
                                                                                                                                           |                                                                                                                                        
  aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGATATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                                                        <  2:4179931/140‑1 (MQ=255)
  aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                                                        <  2:3709634/140‑1 (MQ=255)
  aTTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCgg                                                                                                                                        <  2:5312952/140‑1 (MQ=255)
   ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTGAAATTTGTGTAATTACCCCTGCGCTTTAACAGGTGGTTGTgggg                                                                                                                                       >  1:4055135/1‑139 (MQ=255)
   ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTGTGTAATTAAGCCGGGGCGTGAACACGTGTTTTTGGGc                                                                                                                                       >  1:7139805/1‑140 (MQ=255)
   ttCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGAAAATGATGGAAGCCGTCGCGGAGTCGCTTAAAATTTATATAATTTACCCTCGGCTTGAGCAAGTGTTTTTGGGc                                                                                                                                       >  2:3899483/1‑140 (MQ=255)
    tCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTGTCTAATTACGCCGGCGCTTGTCCAGGGGTTTGTGGGcc                                                                                                                                      >  2:171866/1‑139 (MQ=255)
       gCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                                                                   <  2:1077623/140‑1 (MQ=255)
       gCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                                                                   <  2:4613866/140‑1 (MQ=255)
       gCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAata                                                                                                                                   <  1:3788349/140‑1 (MQ=255)
        cTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATTGAAATAAGCCAGCGCTTGGCCAGGGGGTTGTggggcaaat                                                                                                                                  >  1:6632899/1‑134 (MQ=255)
         tACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATg                                                                                                                                 <  2:2647002/140‑1 (MQ=255)
          aCTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTATCTAAATACCCCAGCGCTTGACCAGGTGTTTTTCGGCaaaaatt                                                                                                                                >  2:7592343/1‑135 (MQ=255)
            ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                              <  2:5465433/140‑1 (MQ=255)
            ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                              <  2:499759/140‑1 (MQ=255)
            ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                              <  2:2457909/140‑1 (MQ=255)
                 gagTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATc                                                                                                                         <  2:640432/140‑1 (MQ=255)
                     ttgttgACATGATGGGGGATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATAGGGATTTATCCAGa                                                                                                                     <  1:2736101/140‑1 (MQ=255)
                     ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGATGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                                                     <  2:3371841/140‑1 (MQ=255)
                      tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAGTTTCTCTAATTACCCCTGCGCTTGTCCAGGGGTTTTTCGGAAATATTGATTTATCCAGaa                                                                                                                    >  2:1828456/1‑140 (MQ=255)
                      tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTATATCATTACCCCGGCGCGTGACCAGCTGTTTTTGGGCCATATTGCTTTATTCAGaa                                                                                                                    >  1:1334997/1‑140 (MQ=255)
                        ttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATg                                                                                                                  <  1:4492833/140‑1 (MQ=255)
                              tgttgGGTGATGATGATATTTCTCTTGTTTTGTTTCAGCAAATGATGGAAGCCGTCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                            <  1:4617902/137‑1 (MQ=255)
                              tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                            <  1:107179/140‑1 (MQ=255)
                              tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                            <  2:7444894/140‑1 (MQ=255)
                               gttgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  2:4507438/138‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGGAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  2:3058945/140‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTACTTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  2:2070213/140‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  1:1558772/140‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  2:29050/140‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  2:4703641/140‑1 (MQ=255)
                               gatgGGGGATGATGAGATTTCTCTTGATGTGTTTCAGCAAATGAAGGAAGCCGGCGCGGAGTCGCTTACCTTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                           <  1:4808370/140‑1 (MQ=255)
                               gatgGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCATTATGGATTTATCCAGAATGTACGGCa                                                                                                           <  1:2278497/140‑1 (MQ=255)
                                  gggggATGATGAGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGTGTCGCTGAGAATTTCGCTCATTCCCGCGGCGCCTGTCCAGGGGTTGGGCGGCCAGATGGGGTGATCCCGACTGTACCGCAccg                                                                                                        >  2:4089557/1‑139 (MQ=255)
                                      gctgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTACTTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                    <  1:5502632/138‑1 (MQ=255)
                                      gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                    <  2:6227030/140‑1 (MQ=255)
                                      gatgagGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                    <  2:3574499/140‑1 (MQ=255)
                                        tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                                                  <  1:2087839/140‑1 (MQ=255)
                                         gatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTGTCGAATTACGCCGGCGGCTGTGCAGGTGTTTGTGGGCAATAATGATTTATACAGAAAGTTAGGCCCCGCTTGCAc                                                                                                 >  2:3759722/1‑140 (MQ=255)
                                          atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCCTTTTGTATAATTAACCCGGCGCGAGACCACGTGTTTGTGGGCCAAATAGAGTTATCCACAAATTACGTCACCACtttaacc                                                                                                >  1:5323456/1‑135 (MQ=255)
                                          atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTGTCAAATTACGCCTGCGCTTTACCAAGTGTTTTTAGGCAAAATGGATTTATCCAGAATTTAAGGCAACACTTGAAcc                                                                                                >  2:1546465/1‑140 (MQ=255)
                                          atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACATTTTATCTAAATAAGCCAGCGCTTTTCCACCTGTTTTTATGCAAGATATATTTATACAGAAATTACGGAACATCTTTCAcc                                                                                                >  2:1147475/1‑140 (MQ=255)
                                          atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTTTGTAATTACGCCAGCGCTTGAACAGGTGGTTTGGGGGAATATTGTGTTATCAAAAATTTAATGCCCCGCTTTCAcc                                                                                                >  1:3493247/1‑140 (MQ=255)
                                          atgaGATGTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAATTTTATAAAATTACCGCTGCGCGTGACCACGTGTGTGTGGGCCAAAAGGATTTTTACAGAATTTAACGCACCTCtttgacc                                                                                                >  2:2357087/1‑135 (MQ=255)
                                             agaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGGCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCttt                                                                                             <  1:997593/140‑1 (MQ=255)
                                             agaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCttt                                                                                             <  2:7400116/140‑1 (MQ=255)
                                               aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAACTTTTGTATAATTACGCCGGCGGTTGTACAGGGGGTTGGAGGCAATATTGGTTTATGCAGAAAGTTCGGCACCTCTTGGACCGTtgt                                                                                           >  2:1584469/1‑140 (MQ=255)
                                                  tctcTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCt                                                                                        <  2:7255329/140‑1 (MQ=255)
                                                  tctcTTGAATTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCt                                                                                        <  1:7329350/140‑1 (MQ=255)
                                                         aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCGGGGGGCa                                                                                 <  2:1915067/140‑1 (MQ=255)
                                                         aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACAATTTGTCTAATTCACCCGGCGCGTGAACACGGGTTTGTGGGGAAAATGTTTTTATACAGAAATTAAGGCACCGCTTTCACCGTTTTGGGGGGGGCa                                                                                 >  2:914780/1‑140 (MQ=255)
                                                                    cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                                                                      <  2:5414964/140‑1 (MQ=255)
                                                                    cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                                                                      <  2:3615412/140‑1 (MQ=255)
                                                                             gAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                                                             <  2:3455836/140‑1 (MQ=255)
                                                                              caGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTGGTGCTGGGGGAAAACGACGGCGGTCTGCCTGCa                                                            <  1:1457950/139‑1 (MQ=255)
                                                                              aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                                                            <  1:7386099/140‑1 (MQ=255)
                                                                              aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                                                            <  1:1019858/140‑1 (MQ=255)
                                                                              aaGCCGGCGCGGAGTCGCTTACAATTTGTCTAATTTCCCCAGCGCTTGTCCCGGGGTTTGTGGGCAATAATGATTTATACAGAATGTTCGGGCCCTCATTGACCGTTGTGGGGGGGGGCAACCACGGGGGTGTGCCTGCa                                                            >  2:5097285/1‑140 (MQ=255)
                                                                                  cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                        <  1:2502532/140‑1 (MQ=255)
                                                                                  cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                        <  1:7531695/140‑1 (MQ=255)
                                                                                  cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                        <  2:6665596/140‑1 (MQ=255)
                                                                                           gTCGCTTACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATCTGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaa                                               <  1:6309656/140‑1 (MQ=255)
                                                                                              gCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATg                                            <  1:5411922/140‑1 (MQ=255)
                                                                                                ttACATTTTCTCTAAGTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGACTGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg                                          <  2:7659934/140‑1 (MQ=255)
                                                                                                   cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                       <  1:3717524/140‑1 (MQ=255)
                                                                                                   cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                       <  1:2681880/140‑1 (MQ=255)
                                                                                                   cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                       <  1:582631/140‑1 (MQ=255)
                                                                                                   cATGTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                       <  2:7018462/140‑1 (MQ=255)
                                                                                                    aTTTTCTCTAATTACGCCTGCGCTTGACCAGGTGTTTTTCGGCAATATGGATTTATCCAGAATGTACCGCACCTCGTTCAACATTGTGCGGGGGGCAAACCAAGGCGGTGTGCCTGCACGCCCGGATGAAAAAGGGGTGc                                      >  2:1606191/1‑140 (MQ=255)
                                                                                                       ttCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGt                                   <  1:1693857/140‑1 (MQ=255)
                                                                                                          tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg                                >  2:2179446/1‑140 (MQ=255)
                                                                                                          tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg                                >  2:611076/1‑140 (MQ=255)
                                                                                                          tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg                                >  2:20172/1‑140 (MQ=255)
                                                                                                             aaTTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACg                             <  1:424958/140‑1 (MQ=255)
                                                                                                                 ccGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGa                         <  1:1936549/140‑1 (MQ=255)
                                                                                                                 cagCCTTCGCTTGACCAGGTGTTTGTCGGAAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGTTGCTGTCGGACGATGa                         <  1:3899483/138‑1 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCACTCTGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGAACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  1:3135611/1‑140 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCTGACGATGAcc                       >  2:4009784/1‑139 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGAcc                       >  2:5793970/1‑139 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  2:6579127/1‑140 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  1:2872457/1‑140 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  2:1432879/1‑140 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  2:4828575/1‑140 (MQ=255)
                                                                                                                    ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                      >  1:6949447/1‑140 (MQ=255)
                                                                                                                        cgcTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGaa                  <  2:7043131/140‑1 (MQ=255)
                                                                                                                        cgcTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGGGCTGTCGGACGATGACCGGGac                  >  2:5690060/1‑139 (MQ=255)
                                                                                                                        cgcTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGCACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGaa                  <  2:4310507/140‑1 (MQ=255)
                                                                                                                             gACCAGGTGTTTGTCGGCAAGATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCt             <  2:5856570/140‑1 (MQ=255)
                                                                                                                                      tttGTGGGCAATCTGGATTTATCCAGTATGTACGGCACCTGTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:555271/140‑1 (MQ=255)
                                                                                                                                      tttGTGGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:6160092/140‑1 (MQ=255)
                                                                                                                                      tttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  2:6820874/140‑1 (MQ=255)
                                                                                                                                      tttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:677042/140‑1 (MQ=255)
                                                                                                                                      tttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:6065973/140‑1 (MQ=255)
                                                                                                                                      tttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGGTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:3014377/140‑1 (MQ=255)
                                                                                                                                      tttGTCGGAAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt    <  1:5090804/140‑1 (MQ=255)
                                                                                                                                        tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAGTGGGGTGCTGTCGGACGATGACAGGGAATGGCTGAAAACGATTg  >  1:3418035/1‑140 (MQ=255)
                                                                                                                                        tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg  >  1:5905577/1‑140 (MQ=255)
                                                                                                                                        tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg  >  1:7618296/1‑140 (MQ=255)
                                                                                                                                        tGTCGGCAATATGGATTTATCAAGAATGTACGGCACCTCTTGCAGCTTTGTGCGGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAACATGGGGGGCTGTCGGACTATGAGCGGGAATGGCTGAAAACGAGTg  >  1:7230057/1‑140 (MQ=255)
                                                                                                                                        tGTCGGCAATATGGATGTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCACACGACGGCGTTCTGCCTGCACGCCCTGATGAAACTGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg  >  1:4882598/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                        
TCATTCAGCTACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTG  >  NZ_CP034943/1111536‑1111811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: