Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,111,684 G→T 8.0% M593I (ATG→ATT addB → helicase‑exonuclease AddAB subunit AddB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,111,6840GT 8.0% 1057.7 / 25.7 371M593I (ATG→ATTaddBhelicase‑exonuclease AddAB subunit AddB
Reads supporting (aligned to +/- strand):  ref base G (107/225);  new base T (33/2);  total (144/227)
Fisher's exact test for biased strand distribution p-value = 3.17e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

TACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAGCT  >  NZ_CP034943/1111545‑1111821
                                                                                                                                           |                                                                                                                                          
   ttGAGGAGTTTGTTGAAGAGTTGGCGGATGCTGAGATTTCTCTTGATTTGGTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTCCCTTTTATCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                                         <  2:5702107/140‑1 (MQ=255)
   ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                                         <  2:1198361/140‑1 (MQ=255)
   ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                                         <  2:5475090/140‑1 (MQ=255)
   ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                                         <  2:4038094/140‑1 (MQ=255)
   ttGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGAt                                                                                                                                         <  2:6384061/140‑1 (MQ=255)
            ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGATGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                                                                <  2:3371841/140‑1 (MQ=255)
            ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                                                                <  2:4177072/140‑1 (MQ=255)
            ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                                                                <  2:291395/140‑1 (MQ=255)
            ttgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGa                                                                                                                                <  1:5528647/140‑1 (MQ=255)
             tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAGTTTCTCTAATTACCCCTGCGCTTGTCCAGGGGTTTTTCGGAAATATTGATTTATCCAGaa                                                                                                                               >  2:1828456/1‑140 (MQ=255)
             tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACAATTTCTGTAATTGCGCCGGCGCGTGACCACGTGTTTGTGGGCCAAATGGATTTATACAGaa                                                                                                                               >  2:677042/1‑140 (MQ=255)
             tgttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTTCATTTTATATCATTACCCCGGCGCGTGACCAGCTGTTTTTGGGCCATATTGCTTTATTCAGaa                                                                                                                               >  1:1334997/1‑140 (MQ=255)
               ttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATg                                                                                                                             <  1:4492833/140‑1 (MQ=255)
               ttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATg                                                                                                                             <  1:1548716/140‑1 (MQ=255)
               ttgAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATg                                                                                                                             <  1:2971074/140‑1 (MQ=255)
                     tgttgGGTGATGATGATATTTCTCTTGTTTTGTTTCAGCAAATGATGGAAGCCGTCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                                       <  1:4617902/137‑1 (MQ=255)
                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                                       <  1:4058469/140‑1 (MQ=255)
                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                                       <  1:107179/140‑1 (MQ=255)
                     tgatgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGc                                                                                                                       <  2:7444894/140‑1 (MQ=255)
                      gttgGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCa                                                                                                                      <  2:4507438/138‑1 (MQ=255)
                      gatgGGGGATGATGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCATTATGGATTTATCCAGAATGTACGGCa                                                                                                                      <  1:2278497/140‑1 (MQ=255)
                         gggggATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAAATACGCCAGCGCATGAACAGGTGTTTTTGGGCAATAATGAATTATACACAAAGTACCGCACct                                                                                                                   >  2:7024618/1‑140 (MQ=255)
                             gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                               <  1:673072/140‑1 (MQ=255)
                             gatgatGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                               <  2:6227030/140‑1 (MQ=255)
                             gatgagGAGAGTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTg                                                                                                               <  2:3574499/140‑1 (MQ=255)
                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                                                             <  2:3740206/140‑1 (MQ=255)
                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                                                             <  1:2087839/140‑1 (MQ=255)
                               tgatgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCa                                                                                                             <  2:7369079/140‑1 (MQ=255)
                                 atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCCTTTTGTATAATTAACCCGGCGCGAGACCACGTGTTTGTGGGCCAAATAGAGTTATCCACAAATTACGTCACCACtttaacc                                                                                                           >  1:5323456/1‑135 (MQ=255)
                                 atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTAAAATTTGTCAAATTACGCCTGCGCTTTACCAAGTGTTTTTAGGCAAAATGGATTTATCCAGAATTTAAGGCAACACTTGAAcc                                                                                                           >  2:1546465/1‑140 (MQ=255)
                                 atgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAAATTTTTTGTAATTACGCCAGCGCTTGAACAGGTGGTTTGGGGGAATATTGTGTTATCAAAAATTTAATGCCCCGCTTTCAcc                                                                                                           >  1:3493247/1‑140 (MQ=255)
                                  tgaGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTATGGAATTACGCCAGCGCATTACCAGGGTTTTGTGGGCAAAATTGATTTATCCACAAAGTAACGCACCTCTTTCAccg                                                                                                          >  2:3796323/1‑139 (MQ=255)
                                    agaTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGGCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCttt                                                                                                       <  1:997593/140‑1 (MQ=255)
                                      aTTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTAACTTTTGTATAATTACGCCGGCGGTTGTACAGGGGGTTGGAGGCAATATTGGTTTATGCAGAAAGTTCGGCACCTCTTGGACCGTtgt                                                                                                     >  2:1584469/1‑140 (MQ=255)
                                        ttCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGc                                                                                                   <  1:5494269/140‑1 (MQ=255)
                                         tctcTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCTGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCt                                                                                                  <  2:7080210/140‑1 (MQ=255)
                                           tctTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTCTCTAATTACCCCAGCGCTTGACCAGGTGTTTGTGGGCAATATGGATTTATCCAGAATGTACGGCACCGCGTGCACCTTTGTGCggg                                                                                                >  2:7524059/1‑140 (MQ=255)
                                                aTTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCGTACAATTTGTCTAATTCACCCGGCGCGTGAACACGGGTTTGTGGGGAAAATGTTTTTATACAGAAATTAAGGCACCGCTTTCACCGTTTTGGGGGGGGCa                                                                                           >  2:914780/1‑140 (MQ=255)
                                                     tttCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTTCATTTTGTGGAATTACGCCGGCGCGTGACCCGGGGTTTTTGGGGCATATTGATTTTTGCAAAATGTACGGCACCACATGGACCGTTTGGGGGGGGGGCaacca                                                                                       >  2:2758670/1‑137 (MQ=255)
                                                          gCAACTGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAAGTCCGCCTGCCCGTGACCAGGTGTTTGTCGGCAATATGGATGTATCCAGAATGTACGGCACCACTTGCCCCTTTGTGCTGGGGGCAAACGACGGCg                                                                                 <  1:2797080/140‑1 (MQ=255)
                                                          gCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCTGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCg                                                                                 <  2:2391983/140‑1 (MQ=255)
                                                          gCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCg                                                                                 <  2:110773/140‑1 (MQ=255)
                                                           cAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGt                                                                                <  2:3615412/140‑1 (MQ=255)
                                                                    gAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                                                                       <  2:3455588/140‑1 (MQ=255)
                                                                     caGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTGGTGCTGGGGGAAAACGACGGCGGTCTGCCTGCa                                                                      <  1:1457950/139‑1 (MQ=255)
                                                                     aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                                                                      <  1:1019858/140‑1 (MQ=255)
                                                                     aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                                                                      <  1:1831999/140‑1 (MQ=255)
                                                                     aaGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCa                                                                      <  1:7386099/140‑1 (MQ=255)
                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                                  <  2:4313803/140‑1 (MQ=255)
                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                                  <  1:7531695/140‑1 (MQ=255)
                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                                  <  2:3108820/140‑1 (MQ=255)
                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                                  <  2:1693273/140‑1 (MQ=255)
                                                                         cGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATAGGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGcc                                                                  <  2:5196090/140‑1 (MQ=255)
                                                                             gcgGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCtga                                                              <  2:7652543/140‑1 (MQ=255)
                                                                                  gTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaa                                                         <  1:6134455/140‑1 (MQ=255)
                                                                                     gCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATg                                                      <  1:5411922/140‑1 (MQ=255)
                                                                                       ttACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg                                                    <  2:465979/140‑1 (MQ=255)
                                                                                          cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                                 <  1:3717524/140‑1 (MQ=255)
                                                                                          cATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTg                                                 <  1:582631/140‑1 (MQ=255)
                                                                                              gtctcTAATTCCGCCTGCGCTTGACCAGGTGTGTGTCGGCAATAGGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGt                                             <  1:73203/139‑1 (MQ=255)
                                                                                               tctcTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTgggg                                                                                              <  2:5434898/90‑1 (MQ=255)
                                                                                               tctcTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTc                                            >  2:6142482/1‑140 (MQ=255)
                                                                                                ctctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCg                                           >  2:17366/1‑140 (MQ=255)
                                                                                                 tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg                                          >  2:2054644/1‑140 (MQ=255)
                                                                                                 tctAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCgg                                          >  2:479320/1‑140 (MQ=255)
                                                                                                     aTTCCGCCTGCGATTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGa                                      <  2:5049857/140‑1 (MQ=255)
                                                                                                        cagCCTTCGCTTGACCAGGTGTTTGTCGGAAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGTTGCTGTCGGACGATGa                                   <  1:3899483/138‑1 (MQ=255)
                                                                                                           ccTGCGCTTGACCAGGTGTTTGTCGGCACTCTGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGAACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                                >  1:3135611/1‑140 (MQ=255)
                                                                                                           ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATggg                                                    >  1:3764168/1‑120 (MQ=255)
                                                                                                           ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                                >  2:1432879/1‑140 (MQ=255)
                                                                                                           ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                                >  2:2326625/1‑140 (MQ=255)
                                                                                                           ccTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCg                                >  2:2139563/1‑140 (MQ=255)
                                                                                                               cgcTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGaa                            >  2:6437842/1‑140 (MQ=255)
                                                                                                               cgcTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCGTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGaa                            >  1:6043205/1‑140 (MQ=255)
                                                                                                                    gACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa                                                        >  1:2566971/1‑107 (MQ=255)
                                                                                                                    gACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGaaaa                                                        <  2:2566971/107‑1 (MQ=255)
                                                                                                                    gACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCt                       <  2:6618190/140‑1 (MQ=255)
                                                                                                                    gACCAGGTGTTTGTCGGCAAGATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCt                       <  2:5856570/140‑1 (MQ=255)
                                                                                                                     aCCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTCCGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTg                      >  1:2699356/1‑140 (MQ=255)
                                                                                                                          gtgtTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAAc                 >  1:4351130/1‑140 (MQ=255)
                                                                                                                          gtgtTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAAc                 >  1:7227281/1‑140 (MQ=255)
                                                                                                                             tttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt              <  1:6273872/140‑1 (MQ=255)
                                                                                                                             tttGTCGGAAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAt              <  1:5090804/140‑1 (MQ=255)
                                                                                                                              ttGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGAtt             >  1:1960858/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                                                                       <  1:6534375/81‑1 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTctgcctgc                                                                       >  2:1927283/1‑81 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAGTGGGGTGCTGTCGGACGATGACAGGGAATGGCTGAAAACGATTg            >  1:3418035/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg            >  1:5905577/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg            >  1:7618296/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg            >  1:7100137/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCGTGGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg            >  1:486455/1‑140 (MQ=255)
                                                                                                                               tGTCGGCAATATGGATGTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCACACGACGGCGTTCTGCCTGCACGCCCTGATGAAACTGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTg            >  1:4882598/1‑140 (MQ=255)
                                                                                                                                    gCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGAtt       >  2:5806584/1‑140 (MQ=255)
                                                                                                                                    gCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGAtt       >  1:3096766/1‑140 (MQ=255)
                                                                                                                                    gCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGAtt       >  2:4951406/1‑140 (MQ=255)
                                                                                                                                       atatTGATTTATCCAGAATGTAAGTCACCTCTTGCACCTTTGTGCTGGGGGAAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGAACGGGAATGGCTGAAAACGATTGGGATTGAg    <  1:2502009/140‑1 (MQ=255)
                                                                                                                                       atatGTATTTATACAGAATGTACGGTACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGGGTTCTGCGTGCAGGCGCTGATGAAAATGGGTTGCTGTGGGACGTTGACCGGGAATGGCTGAATACGATTGGGATTGAg    <  1:1603962/140‑1 (MQ=255)
                                                                                                                                       atatGGATTTATCCAGAATGTACGGCACCTCTTGCCCCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAg    <  2:5939804/140‑1 (MQ=255)
                                                                                                                                       atatGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAg    <  2:6867456/140‑1 (MQ=255)
                                                                                                                                       atatGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAg    <  1:683115/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                          
TACTTGAGGAGTTTGTTGAAATGATGGGGGATGATGAGATTTCTCTTGATTTGTTTCAGCAAATGATGGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCAGGTGTTTGTCGGCAATATGGATTTATCCAGAATGTACGGCACCTCTTGCACCTTTGTGCTGGGGGCAAACGACGGCGTTCTGCCTGCACGCCCTGATGAAAATGGGGTGCTGTCGGACGATGACCGGGAATGGCTGAAAACGATTGGGATTGAGCT  >  NZ_CP034943/1111545‑1111821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: