Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 131,088 C→A 100% S194Y (TCT→TAT)  fusA → elongation factor G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943131,0880CA99.7% 3239.0 / ‑3.0 873S194Y (TCT→TAT) fusAelongation factor G
Reads supporting (aligned to +/- strand):  ref base C (4/3);  new base A (392/473);  total (397/476)
Fisher's exact test for biased strand distribution p-value = 7.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 879 total aligned reads displayed.

TAGGAACTTTAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTCTGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAG  >  NZ_CP034943/130950‑131224
                                                                                                                                          |                                                                                                                                        
        ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCCATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  2:5822235/1‑140 (MQ=255)
        ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  1:5806165/1‑140 (MQ=255)
        ttAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTAGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaa                                                                                                                                 >  2:2650886/1‑140 (MQ=255)
          aaGAGACCGTCTTCAATCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTACAAAACCTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaag                                                                                                                               >  1:1514325/1‑140 (MQ=255)
           agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:5882044/140‑1 (MQ=255)
           agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:1651177/140‑1 (MQ=255)
           agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:6837168/140‑1 (MQ=255)
           agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:7234095/140‑1 (MQ=255)
           agagACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:421359/140‑1 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCCATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  1:5316062/1‑140 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCACTTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  1:5513118/1‑140 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              >  2:3562374/1‑134 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCgaaaga                                                                                                                              <  1:3562374/134‑1 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  2:866374/1‑140 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  1:1922549/1‑140 (MQ=255)
                 cGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATccc                                                                                                                        >  2:7144720/1‑140 (MQ=255)
                  gTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCt                                                                                                                       <  2:4227708/140‑1 (MQ=255)
                  gTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCt                                                                                                                       <  2:3080244/140‑1 (MQ=255)
                   tCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTg                                                                                                                      >  2:1527186/1‑140 (MQ=255)
                     ttCAAGCAACCGCACATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCTTATTTATACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGaa                                                                                                                    <  1:4934968/140‑1 (MQ=255)
                     ttCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGaa                                                                                                                    <  2:1730725/140‑1 (MQ=255)
                     ctCAAGCAAACGGTCATGCACTACACTTGCCGAACGGAGCTTAGGACAACTTCGAAGGTATCATCGTCCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGATCTCGTTATGATGCGAAAGAAATCCCTGaa                                                                                                                    <  2:5720740/139‑1 (MQ=255)
                      tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:1283625/140‑1 (MQ=255)
                      tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:408661/140‑1 (MQ=255)
                      tCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:3132671/140‑1 (MQ=255)
                      tCAAGCAAAAGCTCATGCAATTCAATTCCCGATCTGTGCTGAGGACAACTTCGAAGGTATGATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTAGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:1806519/140‑1 (MQ=255)
                      tCAAGAAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTATACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:5281641/140‑1 (MQ=255)
                            acaCGCGCATGCAATACACGTGCCGATCGGTGCTGAGGACAACCTCGAAGGTATCAACGACCTTGTAGAACACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAACAGTACa                                                                                                             <  2:6368035/138‑1 (MQ=255)
                            aaaCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAACACGGTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACa                                                                                                             <  1:6593553/140‑1 (MQ=255)
                            aaaCGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACa                                                                                                             <  1:385708/140‑1 (MQ=255)
                               cGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  1:3467862/131‑1 (MQ=255)
                                gCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGCAAGAAATCCCTGAAGAGTACAAaga                                                                                                         <  2:4724923/140‑1 (MQ=255)
                                gCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAaga                                                                                                         <  1:272863/140‑1 (MQ=255)
                                gCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAaga                                                                                                         <  2:4544942/140‑1 (MQ=255)
                                  tCATGCAATTCAAGTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGc                                                                                                       <  2:1143271/140‑1 (MQ=255)
                                    aTGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACCAAGAGCaa                                                                                                     >  2:4066841/1‑140 (MQ=255)
                                          ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATcc                                                                                                                         >  2:1278680/1‑114 (MQ=255)
                                          ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  1:1174146/1‑140 (MQ=255)
                                          ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  2:6249068/1‑140 (MQ=255)
                                          ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  1:5198110/1‑140 (MQ=255)
                                          ttcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGaa                                                                                               >  1:4767749/1‑140 (MQ=255)
                                           tcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAag                                                                                              <  1:6685236/140‑1 (MQ=255)
                                           tcaattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAag                                                                                              <  1:2145418/140‑1 (MQ=255)
                                            caattGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAaga                                                                                             <  2:3950646/140‑1 (MQ=255)
                                                     aTCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCaa                                                                                    <  1:2600234/140‑1 (MQ=255)
                                                         gttcTGAGGAAAACTTCGAAGGTATCATCGAACTTGTACAAAACGTTTAGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAATAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  2:1956942/137‑1 (MQ=255)
                                                         gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  1:6345074/140‑1 (MQ=255)
                                                         gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  1:5680398/140‑1 (MQ=255)
                                                         gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  1:3647067/140‑1 (MQ=255)
                                                         gTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGc                                                                                <  1:4348131/140‑1 (MQ=255)
                                                               aGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCAtt                                                                          >  1:463218/1‑140 (MQ=255)
                                                                   cAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAg                                                                      <  2:285813/140‑1 (MQ=255)
                                                                   cAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAg                                                                      <  1:7456240/140‑1 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGTAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:751287/1‑140 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  1:969269/1‑140 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  1:7666508/1‑140 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:1221538/1‑140 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  1:2334104/1‑140 (MQ=255)
                                                                    aaCTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGc                                                                     >  2:4313128/1‑140 (MQ=255)
                                                                           aaGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTtgt                                                              <  1:2015502/140‑1 (MQ=255)
                                                                               tATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGc                                                          <  2:2086094/140‑1 (MQ=255)
                                                                               tATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGc                                                          <  2:3767287/140‑1 (MQ=255)
                                                                                 tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:7219576/81‑1 (MQ=255)
                                                                                 tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  1:2880373/1‑140 (MQ=255)
                                                                                 tcatcGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCtt                                                        >  1:2958198/1‑140 (MQ=255)
                                                                                     cGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTtgatg                                                    <  2:3436155/140‑1 (MQ=255)
                                                                                       aCCTTTTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGATATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGATTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:3224248/140‑1 (MQ=255)
                                                                                       aCCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGTAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:6127671/140‑1 (MQ=255)
                                                                                       aCCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:4829316/140‑1 (MQ=255)
                                                                                       aCCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGaa                                                  <  2:3358839/140‑1 (MQ=255)
                                                                                        ccTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAag                                                                                                                   <  2:2217043/74‑1 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAagaga                                              >  2:5570775/1‑139 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGACGAGc                                              >  2:5054324/1‑140 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  2:7026426/1‑140 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  2:6868467/1‑140 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  1:6429576/1‑140 (MQ=255)
                                                                                           tGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGc                                              >  2:2364742/1‑140 (MQ=255)
                                                                                            gTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCt                                             >  1:3973281/1‑140 (MQ=255)
                                                                                              aGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  2:2958198/140‑1 (MQ=255)
                                                                                              aGAAAACGTTGCGTATGTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTa                                           <  1:4568494/140‑1 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTGGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCACGCTGAAGAGCTTCGCACCAGCGTCATTGATGCTGTGTGTGAGCTTGATGACGAGCTTCTggc                                       >  1:1026896/1‑139 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  2:7602755/1‑140 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  1:6177498/1‑140 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  1:1400485/1‑140 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGa                                       >  2:144813/1‑140 (MQ=255)
                                                                                                  aaCGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTGTGTGAGCTTGATGAAGAGCTTATGGa                                       >  1:7298034/1‑140 (MQ=255)
                                                                                                    cGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATa                                     <  2:5477521/140‑1 (MQ=255)
                                                                                                    cGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATa                                     <  2:2545109/140‑1 (MQ=255)
                                                                                                       tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAag                                                                                              >  1:7565227/1‑80 (MQ=255)
                                                                                                       tGCGTATTTCTACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAAt                                  >  1:6099949/1‑140 (MQ=255)
                                                                                                                 tACGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAggg                        >  1:2462717/1‑140 (MQ=255)
                                                                                                                   cGAAGATGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTg                      <  2:643790/140‑1 (MQ=255)
                                                                                                                        aTGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTgaagaa                 >  2:1833357/1‑140 (MQ=255)
                                                                                                                        aTGACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTgaagaa                 >  1:3302397/1‑140 (MQ=255)
                                                                                                                          gACCTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAAt               <  2:5937128/140‑1 (MQ=255)
                                                                                                                             cTCGGAACTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCAc            >  1:227034/1‑140 (MQ=255)
                                                                                                                                 gAACTCGTTATGATGCTAAAGAAATCCCTGACGAGTACAAACAGAAAGCTGAAGAGCTTCGCAACAGCCTCATTCAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGTGTGAAGAAATCACGAtt        >  1:5030708/1‑140 (MQ=255)
                                                                                                                                   agtCGTTATGATGCGAAATAAATGCCTGAAGAGTACAAAGATAAAGCTGAAGAGCTTCGAAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGa      <  2:6577234/138‑1 (MQ=255)
                                                                                                                                    cTCGTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGAc     <  1:4455626/140‑1 (MQ=255)
                                                                                                                                       gTTATGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAg  >  1:7081085/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                        
TAGGAACTTTAAGAGACCGTCTTCAAGCAAACGCTCATGCAATTCAATTGCCGATCGGTGCTGAGGACAACTTCGAAGGTATCATCGACCTTGTAGAAAACGTTGCGTATTTCTACGAAGATGACCTCGGAACTCGTTCTGATGCGAAAGAAATCCCTGAAGAGTACAAAGAGCAAGCTGAAGAGCTTCGCAACAGCCTCATTGAAGCTGTTTGTGAGCTTGATGAAGAGCTTATGGATAAATACCTTGAGGGTGAAGAAATCACGATTGACGAG  >  NZ_CP034943/130950‑131224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: