Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,152,007 G→C 7.8% L66V (CTT→GTT)  EO946_RS05920 ← GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,152,0070GC 7.8% 972.3 / 9.2 380L66V (CTT→GTT) EO946_RS05920GNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (192/150);  new base C (30/2);  total (228/152)
Fisher's exact test for biased strand distribution p-value = 1.47e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 387 total aligned reads displayed.

AGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGC  >  NZ_CP034943/1151868‑1152146
                                                                                                                                           |                                                                                                                                           
    gCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTGTAAGATTAAGAGTCCGATAGATGCAAGACgg                                                                                                                                         >  1:213055/1‑140 (MQ=255)
    gCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATGACGACTCCGATAGAGGCAAGACgg                                                                                                                                         >  1:4306990/1‑140 (MQ=255)
    gCAAACCTTCTTTTGTGGATAAAATAATTATAGCGGTGCTTTCATACGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTTTATTCTTTGTCTAAGAATAAGAGTACGATAGATTCAAGACgg                                                                                                                                         >  2:6562265/1‑140 (MQ=255)
                   tgGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTTTATTGTTTTTCTAACATGAGGAGTGCGATAGAGGCACCACGGGCACACAAAAAAAAg                                                                                                                          >  1:467923/1‑140 (MQ=255)
                    ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTTTTGTTTTTCTACCATTACGAGTCCCATAGAGGCAACACGGCCaaaaaaaaaaaaac                                                                                                                         >  1:6775133/1‑138 (MQ=255)
                    ggCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGc                                                                                                                         >  2:6201685/1‑140 (MQ=255)
                        cAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTGTATAAGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAAAAAACCGAt                                                                                                                     >  2:3393466/1‑140 (MQ=255)
                          aaGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                                                                   <  2:7380615/140‑1 (MQ=255)
                            gCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAg                                                                                                                 <  2:2586352/140‑1 (MQ=255)
                                gATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTGTTTGTCGACGATGGCGGGTCCGATAGAGGCCAGACGGCCCAACAAAAAAAAGCCGATGCAGGgaa                                                                                                             >  1:2317100/1‑140 (MQ=255)
                                  tAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAaata                                                                                                           <  2:1001163/140‑1 (MQ=255)
                                  tAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAaata                                                                                                           <  1:3702407/140‑1 (MQ=255)
                                     cGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTTTATTGTTTTTATACCATGCAGAGTCCGATTAAGGCAACTAGGCCAAACAAAAACACACCGATTCAGGAAATAATg                                                                                                        >  1:290926/1‑140 (MQ=255)
                                      ggTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGt                                                                                                       <  2:3472840/140‑1 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTGTAACATGACGAGTCCGATTGAGGCAAGAAGGCCAAACAAAAAAAAGCCCATTCAGGAaataatttg                                                                                                      >  1:7225220/1‑137 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCCAACAAAAACAAGCCGATGCAGGAAATAATGta                                                                                                      >  2:3888804/1‑139 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGCTGCAGGAAATAATGtc                                                                                                      >  2:5682066/1‑140 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGc                                                                                                                   >  2:7613056/1‑127 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAAAAAAAAAAAGCCGATGCAGGAAATAATGtc                                                                                                      >  2:3587426/1‑140 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTATTTGTAAACGATAAAGAGTCCCATAGAGGCACGACGGCCCAAAAAAAAAAAACCCCTTCAGGGaataaatta                                                                                                      >  1:6737547/1‑136 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGGATTGTTTGTCTACGATTACGAGTCCGATAGAGGCAACACGGCCCAAAAAAAAAAAACCGATTCAGGAAAAAAAGta                                                                                                      >  1:4471396/1‑139 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATTACGAGTCCGATATAGGCAAGACGGCCAAACAAAAAAAACACGATGCAGGAAATAATGtg                                                                                                      >  2:7094199/1‑139 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTATTTGTCTACGATGACGAGTCCGATAGAGGCAACACGGCCAAACAAAAACAAGCCCATGCAGGAAATAATGtc                                                                                                      >  2:1452396/1‑140 (MQ=255)
                                       gTGCTTTCATCCGTTTGGCTGAGACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTAGTTTTATAAGAAGTCGAATACGCTAGCGGCGACTCGGCCAAAACAAAACAAGCCGGTGCCGGAcataaattg                                                                                                      >  1:3220078/1‑136 (MQ=255)
                                       gTGCTTTCAACCGTATGGCTGATACACGCGTTCACCATCCTCAGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATAACGACTACGATAGAGGCACGACGGCCAAAACAAAACAAGCCCATGCGAGAAAAAATTtc                                                                                                      >  2:1208928/1‑140 (MQ=255)
                                         gCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGtctc                                                                                                    <  2:2896517/140‑1 (MQ=255)
                                          cTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAACACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTcc                                                                                                   >  2:5550904/1‑140 (MQ=255)
                                          cTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGGGTACGATGACCAGTCCGATAGAGGCAAGACGGCCAAACCAAAACAATCCGATGCAGGAAATAATGTCTcc                                                                                                   >  2:192643/1‑140 (MQ=255)
                                             tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                <  1:4722627/140‑1 (MQ=255)
                                             tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                <  1:6562265/140‑1 (MQ=255)
                                             tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                <  1:4069368/140‑1 (MQ=255)
                                             tCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTGTTGGTTGTGTACGATGACGAGTCCGATAGAGGGCAGACCGCCCAACAAAAACAAACCCCTGCAGGGAATAATTTGTCcgcg                                                                                                >  1:878532/1‑140 (MQ=255)
                                             tCAACCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCcgcg                                                                                                <  1:1263904/140‑1 (MQ=255)
                                               aTCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGATTGTTTGTCTACGATGACGAGTCCGCTAGAGGCAACACGGCCAAAAAAAAACAAACCCATGCAGGAAATAATGTCTCCCCGGa                                                                                              >  1:1026304/1‑140 (MQ=255)
                                                  cGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACAt                                                                                           <  2:3121917/140‑1 (MQ=255)
                                                    tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTGTACGATTTCGAGTGCGCTATATGCAAGACGGCCAAACAAAAACAATCCGATGCAGGAAATAATGTCTCCGCCGACAttt                                                                                         >  1:1903289/1‑140 (MQ=255)
                                                    tttGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGGATTCTTTTTCTACGATGAAGTGGCCGAACGAGGCCAGACCGCCAAACAAAAACACGCCGATTCCGGAAAAAAAGTAGCCCCGGACAatt                                                                                         >  1:7546506/1‑140 (MQ=255)
                                                        gCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGAt                                                                                     <  1:1580820/140‑1 (MQ=255)
                                                         cTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGAAGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCCATGCAGGGAATAATTTCTCCGCGGACATTTTGATa                                                                                    >  2:4591787/1‑140 (MQ=255)
                                                            aTACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTTTATTCTTTTTCTAAGATTACGAGTGCGAAAGAGGCAAAAAGGCCAAAAAAAAAAAACCCGAATCATGAAAAAATTTATACGCGGAAAtttttttaaac                                                                                 >  1:5610491/1‑133 (MQ=255)
                                                                  gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGTTTTGTTTTTGTAAGATGACGAGTCCGATAGAGGCAAGAAGGCCAAAAAAAAAAAAGCCCAAGCAGGCAAAAATTTCTCCGCGGACAATTTTATAAACGaaaaa                                                                           >  1:4638124/1‑140 (MQ=255)
                                                                  gcgTTCACCATCCGCCGTCCGAGACCGAGCCGTGTGGATTCTTTGTCTACGCTTACGAGTCCGATATAGGCAAGACGGCCAAAAAAAAAAAATCCGAGTGAGGAAATAAATTCTCCGCCGACAAGTTTATAAACCaaaaa                                                                           >  1:3861482/1‑140 (MQ=255)
                                                                       cACCATCCGCCGTCCGAGACCGAGCCGTGTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAATCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGcc                                                                      >  1:5292095/1‑140 (MQ=255)
                                                                            tCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTGCGATAGAGGCAACAAGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAAACGAAAAACCGCCCCAGt                                                                 >  2:6708790/1‑140 (MQ=255)
                                                                            tCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAACACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGt                                                                 >  2:2119445/1‑140 (MQ=255)
                                                                            tCCGCCGTCCGAGACCGAGCCGTTTGGATTCTTTGTCTACGTAGTCGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGAGTCAGGAAATAATGTCTCCGCGCACATTTTGATAACCCAAAAACCGCCCCGGt                                                                 >  2:1871406/1‑140 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTAGGATGACGAGTCCGATAGAGGAAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATATTGTCTCCGCGGACATTTTGATAACCCAAAAAGCGCCCCTGTTGGa                                                             <  1:5951608/140‑1 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATATTGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                             <  1:5400472/140‑1 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                             <  1:3074260/140‑1 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                             <  2:6849562/140‑1 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                             <  1:6249102/140‑1 (MQ=255)
                                                                                ccgTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGATGCAGGAAATAATGTCGCCGCGGAAATTTTGATAACCGAAAAAGCGCCCCTGTTGGa                                                             <  1:1047500/140‑1 (MQ=255)
                                                                                   tCCGAGACCGAGCCGTTTGTATTATTTGTCTACGATGAAGAGTGCGATAGAGGCAACACGGCCAAACAAAAACAAGCCGGTGCACGAAATAATGTCTCCGCGGCCATTTTGATAACCCAAAAAGCGCCCCTGTTGGACaa                                                          >  2:4196730/1‑140 (MQ=255)
                                                                                     ctccaCCGACCCCTTTGTATTCTTTGTCACCGATGACGAGTCCTATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGc                                                        <  2:5107799/136‑1 (MQ=255)
                                                                                     cgtgaCCGAGCGGTTTGTATTCTTTGTCTACCATTTCGAGTCCGATAGCGGCATGACGGCCAAACAAAAACATGCGGATTCAGGAAATCATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGc                                                        <  2:4549074/137‑1 (MQ=255)
                                                                                             aGCCGTTTGTATTCTTTGTATACGATGCCGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCGGTTGGACAAGCACTGCCAg                                                >  2:4347478/1‑140 (MQ=255)
                                                                                             aGCCGTGTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATTGAGGAAATAATGTCTCCGCGGAAATTTTTATAACCGAAAAAGCGCCCCAGTTTGGCAACCAATGCCAg                                                >  2:3225350/1‑140 (MQ=255)
                                                                                                    tGTTTTCTTTGTCTACGATGACGAGTCTGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGt                                         <  2:5309700/140‑1 (MQ=255)
                                                                                                    tGTATTCTTTGTCTACGATGACGAGTCCGCTAGAGGCAAGACGGCCAAAAAAAACCACCCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGt                                         <  1:1840106/140‑1 (MQ=255)
                                                                                                    tGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGt                                         <  1:6321774/140‑1 (MQ=255)
                                                                                                    tGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGt                                         <  1:218914/140‑1 (MQ=255)
                                                                                                     ttATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGtt                                        <  1:5035206/139‑1 (MQ=255)
                                                                                                     gTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGtt                                        <  1:2880183/140‑1 (MQ=255)
                                                                                                     gTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGtt                                        <  1:5698508/140‑1 (MQ=255)
                                                                                                     gTATACTTTGTCAACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACACGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGtt                                        <  2:5777638/140‑1 (MQ=255)
                                                                                                        ttCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGc                                     <  2:1881802/140‑1 (MQ=255)
                                                                                                           tttGTCTACTATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCATGTTGGACAATCAATGCCAGCTCTTGTTCGCCAt                                  >  2:829161/1‑140 (MQ=255)
                                                                                                             tGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATaa                                >  1:2000612/1‑140 (MQ=255)
                                                                                                             tGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATaa                                >  1:6077/1‑140 (MQ=255)
                                                                                                             tGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATaa                                >  1:5135903/1‑140 (MQ=255)
                                                                                                                cTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCa                             <  2:6405193/140‑1 (MQ=255)
                                                                                                                cTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCa                             <  2:2202167/140‑1 (MQ=255)
                                                                                                                        aCGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCAccc                     >  2:2998264/1‑140 (MQ=255)
                                                                                                                         cGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCg                    >  2:4025239/1‑140 (MQ=255)
                                                                                                                         cGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCg                    >  2:34390/1‑140 (MQ=255)
                                                                                                                         cGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATGTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCg                    >  1:7653358/1‑140 (MQ=255)
                                                                                                                          gAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCgg                   <  2:4948424/140‑1 (MQ=255)
                                                                                                                           aGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGc                                                        <  1:1634790/102‑1 (MQ=255)
                                                                                                                           aGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGc                                                        <  1:478756/102‑1 (MQ=255)
                                                                                                                           aGTCCGATAGAGGCAAGACGGCCAAAAAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGc                                                        >  2:478756/1‑102 (MQ=255)
                                                                                                                             tCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAATCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCtt                >  2:1783595/1‑140 (MQ=255)
                                                                                                                             tCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCtt                >  2:273060/1‑140 (MQ=255)
                                                                                                                             tCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCtt                <  1:4839164/140‑1 (MQ=255)
                                                                                                                                   agagGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGc          <  2:2408219/140‑1 (MQ=255)
                                                                                                                                     agGCAAGACGGCCAAACAAAAACAAGCCGATGCAGTAAATAATGTCTCCGAGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCaa        >  1:6424622/1‑140 (MQ=255)
                                                                                                                                     agGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCaa        <  2:5955163/140‑1 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAATCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGACTGCAAAg      >  2:6035618/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACCTTGTGCTAACCGACAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCAGAATCAGGCCACCCGGCTTGCCTGCAAAg      >  2:4987398/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGcc                                    <  1:69834/110‑1 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  2:925995/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  2:6317387/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  1:3373397/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      <  2:6148759/140‑1 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  1:3483019/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  2:5158729/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  2:2524882/1‑140 (MQ=255)
                                                                                                                                       gCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAg      >  1:846851/1‑140 (MQ=255)
                                                                                                                                        cAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGc     <  2:1084928/140‑1 (MQ=255)
                                                                                                                                          aGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCg   >  2:2679041/1‑140 (MQ=255)
                                                                                                                                           gACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGcc                                    >  1:2565407/1‑106 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGCGGCAAACCTTCTTTTGTGGCGCAAAGCCTGATAGCGGTGCTTTCATCCGTTTGGCTGATACACGCGTTCACCATCCGCCGTCCGAGACCGAGCCGTTTGTATTCTTTGTCTACGATGACGAGTCCGATAGAGGCAAGACGGCCAAACAAAAACAAGCCGATGCAGGAAATAATGTCTCCGCGGACATTTTGATAACCGAAAAAGCGCCCCTGTTGGACAAGCAATGCCAGCTCTTGTTCGCCATAATCAGGCCACCCGGCTTGCCTGCAAAGCCGC  >  NZ_CP034943/1151868‑1152146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: