Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,263,949 G→T 6.1% R79R (CGA→AGA)  fetB ← iron export ABC transporter permease subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,263,9490GT 6.1% 1050.9 / 13.5 378R79R (CGA→AGA) fetBiron export ABC transporter permease subunit FetB
Reads supporting (aligned to +/- strand):  ref base G (188/153);  new base T (4/32);  total (192/186)
Fisher's exact test for biased strand distribution p-value = 2.35e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

CCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAA  >  NZ_CP034943/1263811‑1264086
                                                                                                                                          |                                                                                                                                         
ccATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGt                                                                                                                                          <  2:380496/140‑1 (MQ=255)
     ccGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGAt                                                                                                                                     <  1:5145252/140‑1 (MQ=255)
      cGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATa                                                                                                                                    <  1:1729324/140‑1 (MQ=255)
           aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                               >  1:7065565/1‑140 (MQ=255)
           aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                               >  1:2432081/1‑140 (MQ=255)
           aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                               >  1:5738451/1‑140 (MQ=255)
           aTCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtt                                                                                                                               >  1:2890416/1‑140 (MQ=255)
             cGGAATGACATATCGGGCCGTAAGCTGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGAGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACAtttt                                                                                                                             >  1:3761969/1‑140 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATGGCTAACGCGGCGAACACCCTCCAACACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  2:5089856/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  1:2637449/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  2:5334164/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  1:4430481/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          >  1:3426486/1‑140 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  1:4828234/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  1:5265664/140‑1 (MQ=255)
                aaTGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          <  1:1520652/140‑1 (MQ=255)
                     cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTgcg                                                                                                                          >  1:2114610/1‑135 (MQ=255)
                     cATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCgctgct                                                                                                                     <  1:274329/140‑1 (MQ=255)
                         tCGGGCCGTAAGCGGGAGGATATGAAGAGACAGCAGAAGTCCCTGCGTCACAAGTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                 <  2:1484458/140‑1 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACGGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                >  1:1183254/1‑140 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                >  1:3964029/1‑140 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                >  2:4264973/1‑140 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                >  2:3663101/1‑140 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGc                                                                                                                >  2:2258122/1‑140 (MQ=255)
                          cGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTGGATAACATTTTGCGCTGCTACTGc                                                                                                                >  1:3613936/1‑140 (MQ=255)
                           gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                               <  2:275215/140‑1 (MQ=255)
                           gggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGcc                                                                                                               <  2:6239117/140‑1 (MQ=255)
                            ggCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCa                                                                                                              <  1:607603/140‑1 (MQ=255)
                              ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGACCCGATTGTATTTTTCTTTCGTTTGATAACAGTTTGCGCTGCTACTGCCAAc                                                                                                            >  1:293653/1‑140 (MQ=255)
                              ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATa                                                                                                                                    >  2:3570363/1‑116 (MQ=255)
                              ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                            <  2:1654914/140‑1 (MQ=255)
                              ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                            <  1:6036500/140‑1 (MQ=255)
                              ccGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAAc                                                                                                            >  2:6434458/1‑140 (MQ=255)
                              ccGAAAGCGGGATGATATGAATAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTCTATTTTTCTTTCGTGTGATAACATTTTGCGCTGCTACTGACAAc                                                                                                            >  1:3784426/1‑140 (MQ=255)
                                gTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAt                                                                                                          >  2:6844911/1‑140 (MQ=255)
                                gTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAt                                                                                                          >  2:4618184/1‑140 (MQ=255)
                                 tAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAtt                                                                                                         <  2:4060649/140‑1 (MQ=255)
                                 tAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAtt                                                                                                         >  2:6128199/1‑140 (MQ=255)
                                    gCGGGATGATATGAATAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAAc                                                                                                      <  2:6523984/140‑1 (MQ=255)
                                     cGGGATTATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACa                                                                                                     <  2:403184/140‑1 (MQ=255)
                                      gggATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAg                                                                                                    <  1:81537/140‑1 (MQ=255)
                                       ggATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGc                                                                                                   >  1:2486583/1‑140 (MQ=255)
                                       ggATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGc                                                                                                   >  2:1411785/1‑140 (MQ=255)
                                                gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                          <  2:7099728/140‑1 (MQ=255)
                                                gAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCCAACGCGGCGAACACCCTCCACCCCGAACCGATTGTATTTTTCTTTCGTTTGATAACATTGTGCGCTGCTACTGCCAACATTAACAGCACCATCAGc                                                                                          <  2:7013036/140‑1 (MQ=255)
                                                  agagACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCaa                                                                                        >  2:5546220/1‑140 (MQ=255)
                                                    agaCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGa                                                                                      >  2:767416/1‑140 (MQ=255)
                                                      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                 <  1:2541415/111‑1 (MQ=255)
                                                      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTg                                                                                                                 >  2:2541415/1‑111 (MQ=255)
                                                      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATa                                                                                    >  1:7524058/1‑140 (MQ=255)
                                                      aCAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACAGTAACAGCACCATCAGCAAGATa                                                                                    >  2:6665659/1‑140 (MQ=255)
                                                       cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                                   <  1:3184596/140‑1 (MQ=255)
                                                       cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                                   >  2:3352446/1‑140 (MQ=255)
                                                       cagcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaa                                                                                   >  2:6933041/1‑140 (MQ=255)
                                                        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACCTTAACAGCACCATGAGCAAGATaaa                                                                                  >  2:1271867/1‑140 (MQ=255)
                                                        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaa                                                                                  <  1:4693414/140‑1 (MQ=255)
                                                        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATaaa                                                                                  <  1:5630257/140‑1 (MQ=255)
                                                        agcagAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACGGCCAACATTAACAGCACCAGCAGCAAGATaaa                                                                                  >  2:2430671/1‑140 (MQ=255)
                                                           agAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAAc                                                                               <  1:6313157/140‑1 (MQ=255)
                                                               ttCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGa                                                                           >  2:4830235/1‑140 (MQ=255)
                                                                  ccTGCGTCACAATTTCTACAATCGCTAACGCGGCGTACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAACACAGGATgg                                                                        >  2:3192249/1‑140 (MQ=255)
                                                                  ccTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAAAAGCACCATCAGCAAGATAAAAACAGGATgg                                                                        >  1:6906701/1‑140 (MQ=255)
                                                                                 ccaCAATCCATAACGCGGCTAACACCCTCCAAACCGCACCTATTTTTATTTTCTTTCTTTTGATAACATTTTGGGCGGCTACTGCAAACATTAAAAGAACAATCAGCAAGATAAAAACTGTTTGGTCTCCGCGAAAAATa                                                         <  2:2465348/138‑1 (MQ=255)
                                                                                      ctCGCTAACGCGGCTACCACCACCCAAACCGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACAATCAGCAAGATAAAAACAGGATTGTCTCCGCGAAAAATAACCGa                                                    <  2:7450321/139‑1 (MQ=255)
                                                                                      ctCGCTAACGCGGCTAACACCCTCCAAAAGGAACCTTTTTTATTTTTCTTTGGTTTTATAACTTTTTGGGCTTCTAGTGCCAACATTAACAGCACCATCAGCAAGATAAAAAATGGATGGTCTCGGCTAAAAATAAGCGa                                                    <  2:2320386/139‑1 (MQ=255)
                                                                                      aTCGCTAACGCGGCTAACACCCCCAAAAACCAACCTTTTTTATTTTTCTTTCGTTTGATAAAATTTTGCGCTGATACTGCAAACATTAACAGCACAATCAGAAAGATAAAAACAGGATGGTCTCCGGGAAAAATAAGCGa                                                    <  2:1226309/140‑1 (MQ=255)
                                                                                      aTCGCTAACGCGGCGAACAGCCTACAACACGAACCGATTGTATTTTTCTTTCGTTTGATAACATATTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAACAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                    <  2:5673779/140‑1 (MQ=255)
                                                                                      aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTGGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGAGGGTCTCCGCGAAAAATAAGCGa                                                    <  2:7145410/140‑1 (MQ=255)
                                                                                      aTCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGa                                                    <  2:7268271/140‑1 (MQ=255)
                                                                                      aTCGCTAACGCGGCGAACACCCCCCAAACCGACCCGATTGTATTTTTCTTGCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACCGCACCAACAGCAAGATAAAAACAGGTGGGTCTCCGCGAAAAATAAGCGa                                                    <  2:4757195/140‑1 (MQ=255)
                                                                                            aaCGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGCCAGTAc                                              >  2:5503374/1‑140 (MQ=255)
                                                                                                 ggctAAAACCCTCAAAAACTCACCTATTGTATTTTTATTTCTTTTGATAACATTTTGCGTTGCTAGTGCAAACATTAACAGCACCATCAGCAATATAAAAACCGGATGGTCTCCGCGAAAAATACGCGACAGTACATAAc                                         <  2:3109006/140‑1 (MQ=255)
                                                                                                 ggcgAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAAc                                         <  2:7524058/140‑1 (MQ=255)
                                                                                                 ggcgAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAAc                                         >  1:5498444/1‑140 (MQ=255)
                                                                                                         cccGCCAAACCGACCCGGTTGTATTTTTCTTTCGTTTGATAACATTTTCCGCTGCTAGTCCCAAGATTAACAGCACCTTCAGCAAGATAAAAACTGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATa                                 <  2:5774032/140‑1 (MQ=255)
                                                                                                         ccatCCAAAACGAACTTTTTTTATTTTTCTTTCTTTTTATAACTTTTTGCGCTGCTACTGCCAACTTAAACACCACCATCAGAATCATAAAAACCGGTTGGTCTCCGCTAAAAATACGCGACAGTACATAACCGATGATa                                 <  1:4901156/137‑1 (MQ=255)
                                                                                                           cTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAGAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAag                               >  1:4275274/1‑140 (MQ=255)
                                                                                                           cTCAAAAACGAACCGATTTTATTTTTCTTTCGTTTTATAACTTTTTGCGCTGATACTGCAAACATTAACTGCACCATCAGAAAGATAAAAACAGGAGGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAag                               <  2:1571777/140‑1 (MQ=255)
                                                                                                            tcaaaaaCGAACCTTTTTTTTTTTTCTTTCGTTTTATAACATTTTGGGCTGATACTCCAAAAATTAACACAACAATCACAAATATAAAAACAGGTTGTTCTCCGCCAAAAAAAAGCGACAGTACATAACCGATGATAagc                              <  1:6475702/137‑1 (MQ=255)
                                                                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                              >  2:1015838/1‑140 (MQ=255)
                                                                                                            tCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagc                              >  1:3379429/1‑140 (MQ=255)
                                                                                                             ccAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagca                             >  1:7462517/1‑140 (MQ=255)
                                                                                                              caacccGAACCGTTTGTTTTTTTCTTTCGTTTTATAACTTTTTGCGCGGCTACTGCCAACATTAACAGCACCATCCGCAAGATAAAAACCGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAagcag                            <  2:2890416/135‑1 (MQ=255)
                                                                                                                    gAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAc                      >  2:590612/1‑140 (MQ=255)
                                                                                                                    gAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAc                      >  2:1918331/1‑140 (MQ=255)
                                                                                                                    cacccGATTTTATTTTAATTTCGTTTTATAACATTTGGCGCTGCTACTCCCAACATTAACCGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTAc                      <  1:7341635/137‑1 (MQ=255)
                                                                                                                      aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAATCGAAAGTACATAACCGATGATAAGCAGCTGTACCg                    >  1:1370238/1‑140 (MQ=255)
                                                                                                                      aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                    >  1:4152887/1‑140 (MQ=255)
                                                                                                                      aCCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCg                    >  1:1921741/1‑140 (MQ=255)
                                                                                                                         gATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTg                 >  1:2297285/1‑140 (MQ=255)
                                                                                                                               aTTTTTGTTTCGTTTGATAACTTTTTGCGCTGCGACTGCCAAATTTAACCGCACCATCACCAACATAAAACCAGGAGGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGa           <  1:7236278/140‑1 (MQ=255)
                                                                                                                               aTTTTTGTTTCGTTTCATAACATTTTGCGCGCCTACTCCCAAAATTAACACCACCATCACCAATATAAACACAGGTTGTTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGa           <  1:5850413/140‑1 (MQ=255)
                                                                                                                               aTTTTTCTTTCGTTTTATAACATTTTGCGCTGCTACTGCCAACATTACCCGCACCATCCGCAAGATAAAAACAGGTTGTTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGa           <  2:2984146/140‑1 (MQ=255)
                                                                                                                                 ttttttttGTTTTTATAACATTTGGCGCTGATACTCCAAACATAAACCCCACCACCACAAATATAAAAACTGGTTGGTCGCCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtg         <  1:1015838/140‑1 (MQ=255)
                                                                                                                                  tttctttcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtgg        <  2:4152887/140‑1 (MQ=255)
                                                                                                                                   ttctttcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtggt       >  1:1622566/1‑140 (MQ=255)
                                                                                                                                   ttctttcGTTTGATAACATTGTCCGCTGCTACTCCCAACATTAAAAGCAACATCAGAAAGATAAAACCAGTATGGACTCCGCTAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGAtggt       <  2:2162078/140‑1 (MQ=255)
                                                                                                                                       ttcTTTTTATAACATTTTGCGCTCCTACTCCAAAAATTAACACAACCAACACCAATAAAAAAACAGTATGGTCACCGCCAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa   <  1:6591995/140‑1 (MQ=255)
                                                                                                                                       ttcGTTTGATAACATTTTGCGCTGCTACTGCCAACTTTAACAGCACCATCAGAAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCa   <  2:2613685/140‑1 (MQ=255)
                                                                                                                                        tcGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCaa  >  1:3117706/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CCATTCCGCTGATCGGAATGACATATCGGGCCGTAAGCGGGATGATATGAAGAGACAGCAGAATTCCCTGCGTCACAATTTCTACAATCGCTAACGCGGCGAACACCCTCCAAAACGAACCGATTGTATTTTTCTTTCGTTTGATAACATTTTGCGCTGCTACTGCCAACATTAACAGCACCATCAGCAAGATAAAAACAGGATGGTCTCCGCGAAAAATAAGCGACAGTACATAACCGATGATAAGCAGCTGTACCGCTGCCCGGATGGTGGCAA  >  NZ_CP034943/1263811‑1264086

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: