Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,293,314 C→T 5.2% C129C (TGC→TGT EO946_RS06740 → sigma‑70 family RNA polymerase sigma factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,293,3140CT 5.2% 740.8 / 10.8 261C129C (TGC→TGTEO946_RS06740sigma‑70 family RNA polymerase sigma factor
Reads supporting (aligned to +/- strand):  ref base C (173/62);  new base T (2/11);  total (176/85)
Fisher's exact test for biased strand distribution p-value = 3.67e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 261 total aligned reads displayed.

CATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACG  >  NZ_CP034943/1293176‑1293451
                                                                                                                                          |                                                                                                                                         
catcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCt                                                                                                                                          >  2:691289/1‑140 (MQ=255)
 atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                                         <  2:5897249/140‑1 (MQ=255)
 atcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                                         >  2:503596/1‑140 (MQ=255)
  tcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCttt                                                                                                                                        >  1:392214/1‑140 (MQ=255)
  tcGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCttt                                                                                                                                        >  1:2156718/1‑140 (MQ=255)
     aaTCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGACAAAGAAATGTTTTTGCTGCATCAGGTAGAATGCTTTTCt                                                                                                                                     >  2:4220780/1‑140 (MQ=255)
     aaTCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCt                                                                                                                                     >  2:1807921/1‑140 (MQ=255)
     aaTCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCt                                                                                                                                     >  2:6921127/1‑140 (MQ=255)
             tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                             <  2:7652078/140‑1 (MQ=255)
             tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                             <  2:299999/140‑1 (MQ=255)
             tCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACg                                                                                                                             <  2:7162946/140‑1 (MQ=255)
                aGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAAt                                                                                                                          >  1:341096/1‑140 (MQ=255)
                 gCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATc                                                                                                                         >  2:114207/1‑140 (MQ=255)
                  cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                                        <  1:762735/140‑1 (MQ=255)
                  cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                                        <  1:2389327/140‑1 (MQ=255)
                  cACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                                        <  1:1474028/140‑1 (MQ=255)
                      atgatgGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGa                                                                                                                    <  2:3684399/140‑1 (MQ=255)
                      atgatgGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGa                                                                                                                    <  2:5060926/140‑1 (MQ=255)
                            gTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCtt                                                                                                                                         >  1:937865/1‑113 (MQ=255)
                               gAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCgg                                                                                                           >  1:3486240/1‑140 (MQ=255)
                                aGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGgt                                                                                                          >  1:327240/1‑140 (MQ=255)
                                   cggacggaCAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTa                                                                                                       <  2:1535272/140‑1 (MQ=255)
                                    ggacggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaa                                                                                                      >  2:4343482/1‑140 (MQ=255)
                                     gacggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaa                                                                                                     >  2:2343808/1‑140 (MQ=255)
                                      acggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaa                                                                                                    >  1:2002482/1‑140 (MQ=255)
                                       cggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaaa                                                                                                   >  1:2969875/1‑140 (MQ=255)
                                       cggacAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTaaaaa                                                                                                   >  2:2880520/1‑140 (MQ=255)
                                                 tCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACtgt                                                                                         >  1:7257732/1‑140 (MQ=255)
                                                    gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                      >  2:6138652/1‑140 (MQ=255)
                                                    gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                      >  2:2767746/1‑140 (MQ=255)
                                                    gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                      >  1:6336677/1‑140 (MQ=255)
                                                    gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                      >  1:275931/1‑140 (MQ=255)
                                                    gaagaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCa                                                                                      >  1:4121349/1‑140 (MQ=255)
                                                      atatgaCAGACCGAGACTCCAAGACGCTTTATCCCTTTTACGGGGAAGACAAAAATAAATGTTTTTGCTGAATACGGTTTATTGATTTTATTATGAACGAATCGCTTATTTTCTGGTTGTAAAAAAATCGACTGTGCaaa                                                                                    <  2:2489911/136‑1 (MQ=255)
                                                       taagaCAGATTGATAATCAAAGATGGTTTATCCGTTTTAAGGGGCATATAAAAACAAATTTTTTTGCTGAATAAGTTACAATGCTTTTCTTATTAACTAATCGCTGTTCTTCTCGTTTTAAAAAAATCGACTGTGCAAAc                                                                                   <  2:33751/139‑1 (MQ=255)
                                                       gaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTCAAAAAATCGACTGTGCAAAc                                                                                   >  1:6102515/1‑140 (MQ=255)
                                                       gaagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAAc                                                                                   >  1:6045128/1‑140 (MQ=255)
                                                        atgaCAGAGAGAGAATACAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTGTTGCTGCTTAAGGTAGAATGCTTTTCTTATCAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                  >  1:1875327/3‑140 (MQ=255)
                                                        aagaCAGAGATAGAATTCGAGAAGCATTAGCCCTGTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCaaaag                                                                                  >  2:6510846/1‑138 (MQ=255)
                                                        aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                  >  1:1325915/1‑140 (MQ=255)
                                                        aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                  >  1:1085454/1‑140 (MQ=255)
                                                        aagaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACg                                                                                  >  2:7019683/1‑140 (MQ=255)
                                                         agaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGa                                                                                 >  2:5639420/1‑140 (MQ=255)
                                                         agaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGa                                                                                 >  2:538374/1‑140 (MQ=255)
                                                          gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                                >  1:6404198/1‑140 (MQ=255)
                                                          gaCAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAc                                                                                >  1:6896316/1‑140 (MQ=255)
                                                               agagagaATCCAAGAAGCGTTATCCGTGTTAAGGGGCAGAGAACATGAAATGTTTTTGCTGCCTACGGTAGAATGCTTTTCTTGTGAACGAATCGCTGATCTTCTCGGTGTAAAAAGATCGTCTGTGCAACCGACCagcc                                                                           >  2:4141515/1‑137 (MQ=255)
                                                               agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCCGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATTGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                           >  1:3070323/1‑140 (MQ=255)
                                                               agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                           >  2:3486288/1‑140 (MQ=255)
                                                               agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                           >  2:3611402/1‑140 (MQ=255)
                                                               agagagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCa                                                                           >  1:33751/1‑140 (MQ=255)
                                                                  gagaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAAc                                                                        >  2:2921053/1‑140 (MQ=255)
                                                                   agaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACg                                                                       >  1:1859522/1‑140 (MQ=255)
                                                                   agaATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACg                                                                       >  1:5949482/1‑140 (MQ=255)
                                                                     aaTCAAAGACGCGTTATCCCTGTTAACGGGCAGATAAAAATAAATGTTTTTGCTGAATAAGGTCGAATGCTTTTCTTATGAACCAATCGCTGATGTTCTGGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACggg                                                                     <  2:5546427/140‑1 (MQ=255)
                                                                         cAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCg                                                                                                                        <  2:5961124/85‑1 (MQ=255)
                                                                           agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCATACGGGCGATCg                                                               >  2:3735960/1‑140 (MQ=255)
                                                                           agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCACCAAACGGGCGATCg                                                               >  2:2001033/1‑140 (MQ=255)
                                                                           agaagCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTAGGAACGACTCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCg                                                               >  2:7256350/1‑140 (MQ=255)
                                                                               gatttAACCCTGTTAAGGGGCAGATAAAAAGAACTTTTTTTGCTGAATAAGGTAGATTGATTTTCTTAGGAACGAACCGCTGATCTTCTGGGTTAAAAAAACTCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                           <  1:864166/137‑1 (MQ=255)
                                                                               gCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTaaa                                                           <  2:6404198/140‑1 (MQ=255)
                                                                                  ttATCCCTGTTAAGGGGCAGATAAAAATAATTTTTTTTGCTGCATATGGTATAATGCTTTTCTTATGACCTAATCGGTTATCTTATGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGAt                                                        <  2:2108347/140‑1 (MQ=255)
                                                                                    aTCCCTGTTAAGGGGCAGATAAAAATAAATGTTTTTGATGCTTAAGTTAGAATGCTTTTCTTATTAACTAATCGCTGATCTTCTGGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                                      <  2:1310134/140‑1 (MQ=255)
                                                                                    aTCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                                      >  2:2598267/1‑140 (MQ=255)
                                                                                    aTCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGc                                                      >  2:5460810/1‑140 (MQ=255)
                                                                                     ccctttttAACGGGCATATAAAAATAATTTTTTTTGCTGAATATGTTAGATTGGTTTTCTTATCACCCAATCGCTTATGTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCa                                                     <  1:2529201/134‑1 (MQ=255)
                                                                                       ccTTTTAACGGGCATATAAAACTAAATGTTTTTGCTGCATAAGGTATAATGGTTTTCTTATTAACTAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCaaa                                                   <  2:5177009/140‑1 (MQ=255)
                                                                                          gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGAGCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                >  1:1718476/1‑140 (MQ=255)
                                                                                          gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGAc                                                >  1:6659696/1‑140 (MQ=255)
                                                                                          gTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGAAAACGACCATCAAACGGGCTATCGTAAAGATGCAAAGAc                                                >  1:4566196/1‑140 (MQ=255)
                                                                                          gTTAACGGACAGAGAAAAGGAAATGTTTTTGCTGCATAAGGTAGAATGTTTTTCTTATGAACGGATCGCCGATCTTCTCGGCGTAAAAAAATCGACAGTGCAAACGACGATTAAACGGGCGAGGTTAAAGATGCAAAGAc                                                >  1:5784413/1‑140 (MQ=255)
                                                                                                  cactacaaaaaGAAATGTTTTTGATGAATACGGTTAAAGGTTTTTCTTATCAACGAATCGATTATATTCTCGGTGTAAAAAAATCGAAGGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCaggag                                        <  2:6191972/134‑1 (MQ=255)
                                                                                                   cAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATAGACTGTGCAAACGACCATCAAACGGGCGATCGTCAAGAAGCAAAGAAAGCcggagg                                       >  2:3882411/1‑140 (MQ=255)
                                                                                                    atataaaatGAAATGTTTTTGCTGCATAAGTTATAATGATTTTCTTATTAACTAATCGCTGATATTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAggagga                                      <  1:5639420/136‑1 (MQ=255)
                                                                                                     gagaaagagaaaTGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTAACGGTGTAAAAACATCGACTGTGCAATCGACCATCAAACGGGCGCTCGTAAAGATGCACAGACAGCCGGAGCaa                                     >  2:6015020/1‑140 (MQ=255)
                                                                                                     gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                     >  1:5033605/1‑140 (MQ=255)
                                                                                                     gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                     >  1:4794055/1‑140 (MQ=255)
                                                                                                     gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                     >  1:7483681/1‑140 (MQ=255)
                                                                                                     gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGaa                                     >  2:5572485/1‑140 (MQ=255)
                                                                                                     gagaAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGGACAGATGCAAAGACAGCAGGAGGaa                                     >  1:320683/1‑140 (MQ=255)
                                                                                                            ataattGTTTTGGCTTCATAAGGTATAAGGCTTTTCTTATGAACGAATGGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatc                              <  1:1807921/135‑1 (MQ=255)
                                                                                                            ataaaTGTTTTTGCTGCATAAGGTCTACTGCTTTTATTATCAACGAATCGCTGATATTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatc                              <  1:6921127/138‑1 (MQ=255)
                                                                                                                aTGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatcgatc                          >  1:3791624/1‑140 (MQ=255)
                                                                                                                aTGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAatcgatc                          >  1:1180650/1‑140 (MQ=255)
                                                                                                                   tttttGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGTTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                       <  1:5086667/140‑1 (MQ=255)
                                                                                                                   tttttGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                       <  1:4343482/140‑1 (MQ=255)
                                                                                                                   tttttGCTGCATAAGGTACAATGCTTTTCTTATCAACCACTCGCTGATCTTATCGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                       <  1:114207/140‑1 (MQ=255)
                                                                                                                   tttttGCCGCATACGGTACATTGTTTTTCTTATGACCGAATGGCTGATTTTCCCGGTGAAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCt                       <  1:2107468/140‑1 (MQ=255)
                                                                                                                        gctgcATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACAATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCt                  >  1:7057865/1‑140 (MQ=255)
                                                                                                                         ctgaATATGGTATAATGCTTTTCTTATGACCGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCACACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTg                 <  1:3659638/140‑1 (MQ=255)
                                                                                                                            cATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGaaa              >  2:4532939/1‑140 (MQ=255)
                                                                                                                            cATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGaaa              >  2:165656/1‑140 (MQ=255)
                                                                                                                              tatggTATAATGCTTTTTTTATGACCGACTCGCTTATCTTCTGGGTTTAAAAAAATCGAATGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGc            <  2:4902082/137‑1 (MQ=255)
                                                                                                                                   tAGAATGTTTTTCTTATGAACGAATCGCTTATCTTCTGGGTTTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGGATCGATCGCTTGCCTGAAAGCTTGTc       <  1:5296729/140‑1 (MQ=255)
                                                                                                                                    aGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                          >  1:3238139/1‑104 (MQ=255)
                                                                                                                                    aGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCagg                                          <  2:3238139/104‑1 (MQ=255)
                                                                                                                                      aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa    >  1:5488184/1‑140 (MQ=255)
                                                                                                                                      aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa    >  1:7338129/1‑140 (MQ=255)
                                                                                                                                      aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa    >  2:1189529/1‑140 (MQ=255)
                                                                                                                                      aaTGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCACGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATa    >  1:5563924/1‑140 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CATCGAATCATTTTCCAGCACGATGATGGTTGAGCCGGACGGACAGGTATCAGAAGAAGACAGAGAGAGAATCCAAGAAGCGTTATCCCTGTTAACGGGCAGAGAAAAAGAAATGTTTTTGCTGCATAAGGTAGAATGCTTTTCTTATGAACGAATCGCTGATCTTCTCGGTGTAAAAAAATCGACTGTGCAAACGACCATCAAACGGGCGATCGTAAAGATGCAAAGACAGCAGGAGGAAATGAATCGATCGCTTGCCTGAAAGCTTGTCATACG  >  NZ_CP034943/1293176‑1293451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: