Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,512,548 A→T 5.7% intergenic (‑76/‑37) EO946_RS07905 ← / → typA YlaF family protein/translational GTPase TypA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,512,5480AT 5.7% 857.6 / 6.0 268intergenic (‑76/‑37)EO946_RS07905/typAYlaF family protein/translational GTPase TypA
Reads supporting (aligned to +/- strand):  ref base A (176/69);  new base T (2/18);  total (180/88)
Fisher's exact test for biased strand distribution p-value = 5.49e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

AATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCC  >  NZ_CP034943/1512416‑1512687
                                                                                                                                     |                                                                                                                                            
aataCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCGTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGaac                                                                                                                                      >  2:5080945/1‑139 (MQ=255)
aataCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGTATGTGTGGTATAATGTGaaa                                                                                                                                      >  2:2998273/1‑140 (MQ=255)
  taCTGAAAACCGCTGCAACTGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCGTATGATAAGTTTACAGAAAAAGGTGAAGCTTTTAAAGAGGATGTGTTGTATAATGTGAAAGt                                                                                                                                    >  1:3332766/1‑140 (MQ=255)
  taCTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGt                                                                                                                                    >  1:2537395/1‑140 (MQ=255)
     tGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAATAGGATGTGTGGTATAATGTGAAAGTTAt                                                                                                                                 >  2:5996563/1‑140 (MQ=255)
     tGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTAt                                                                                                                                 >  1:5582109/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCCTAAGTTTACAGAAAAACGTGTCGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTAtgt                                                                                                                               >  1:3884269/1‑138 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  2:2731472/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:5928913/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:5395093/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  2:3140177/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:4216368/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:365648/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:6899757/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:7320967/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:3004522/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:2855084/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  2:1137869/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:171183/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  2:1807100/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTGTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:4485034/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTGTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCt                                                                                                                               >  1:743028/1‑140 (MQ=255)
       aaaaCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTGTTACGTCTTATCATAAGTTTACAGACAAACGTGACGCTTTTCAAGAGGATGTGTGGTATAATGTGAAAGTTAtat                                                                                                                               >  2:3904412/1‑138 (MQ=255)
       aaaaCCGCTGCAAATGAAAATAATAGTAAAAGCCAATTCATTTTTTCATATTTTCGATGCCCTTTTAAGTCTTATCATAAGTTTACAGAAAAACGTGACTCTTTTAAATATGATGTGTGGTATAAGGTGAAAGTTATCTa                                                                                                                              <  2:1512220/140‑1 (MQ=255)
        aaaCCGCTGCAAATGCAAATAATAGTAAAAGACAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTa                                                                                                                              <  2:3566510/140‑1 (MQ=255)
          aCCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAt                                                                                                                            >  1:2298063/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTCAttt                                                                                                                          >  2:5561232/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  1:6423532/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  2:6166441/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  1:6231892/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  2:263599/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  2:4637088/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  1:3178869/1‑140 (MQ=255)
            cGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGATTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAAttt                                                                                                                          >  2:5207433/1‑140 (MQ=255)
              cTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTtta                                                                                                                        >  2:6624524/1‑140 (MQ=255)
                gcaaatgcaaatAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTttatt                                                                                                                      <  2:2973604/140‑1 (MQ=255)
                   aatgcaaatAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAgg                                                                                                                   <  2:74670/140‑1 (MQ=255)
                     tgcaaatAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGgag                                                                                                                 <  2:222201/140‑1 (MQ=255)
                                gTACAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTGACAGAGGCTGTGTGGGATAATGTGAACGTTCTCTCATTTTATGAGGAGATGTCAAAGTg                                                                                                      >  2:3975491/1‑140 (MQ=255)
                                gTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTg                                                                                                      >  1:3562712/1‑140 (MQ=255)
                                gTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGATTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAACAGTg                                                                                                      >  1:6061829/1‑140 (MQ=255)
                                      gCCAATTGATTTTTTTCATATTTTCCATCCCCTTTTACGTCTAATCATATTTTTAAATACAAACTTTACTCTTTTAAAGAGGATGTTTGGTATAATGTGAAAGTTATATAATTTAATTACGACATGAAAAAGTGAAACtt                                                                                                >  1:6792039/1‑140 (MQ=255)
                                      gCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACtt                                                                                                >  2:7090455/1‑140 (MQ=255)
                                           ttCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGACAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGaaa                                                                                           >  1:7534566/1‑140 (MQ=255)
                                               tttttttCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGAt                                                                                       <  2:5342439/140‑1 (MQ=255)
                                                           tttCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATc                                                                           <  1:7090455/140‑1 (MQ=255)
                                                            ttCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAAGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATcg                                                                          <  1:5681130/140‑1 (MQ=255)
                                                               cATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGa                                                                       <  2:7338134/140‑1 (MQ=255)
                                                                aTCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGTATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGat                                                                      <  2:5887139/140‑1 (MQ=255)
                                                                aTCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGat                                                                      <  2:1345308/140‑1 (MQ=255)
                                                                aTCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGat                                                                      <  2:353612/140‑1 (MQ=255)
                                                                 tCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGatt                                                                     <  1:3473816/140‑1 (MQ=255)
                                                                     cTTTTACGTATTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTg                                                                 <  2:1029632/140‑1 (MQ=255)
                                                                     cTTTTAAGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTg                                                                 <  2:324337/140‑1 (MQ=255)
                                                                       tttACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                               <  2:7565469/140‑1 (MQ=255)
                                                                               ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                       >  2:3680709/1‑140 (MQ=255)
                                                                               ttATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGa                                                       >  1:636296/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCTCGATTATTGCCCACGTTGAc                                                      >  2:7283084/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTTCCCACGTTGAc                                                      >  2:5688348/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                      >  2:5840806/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                      <  1:5126612/140‑1 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                      >  1:4052505/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                      >  2:7252831/1‑140 (MQ=255)
                                                                                tATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGAc                                                      >  2:885856/1‑140 (MQ=255)
                                                                                  tCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGAGTCTTGCCCACGTGGTCCa                                                    >  1:6124879/1‑140 (MQ=255)
                                                                                        gTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGc                                                                <  1:1064815/122‑1 (MQ=255)
                                                                                         tttACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCTAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGaaa                                             >  2:1940060/1‑140 (MQ=255)
                                                                                         tttACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGaaa                                             >  2:2367280/1‑140 (MQ=255)
                                                                                         tttACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACAATGGGaaa                                             >  2:6947247/1‑140 (MQ=255)
                                                                                         tttAAATAAAAACGTGAAGATTTTAAAGATTTTGTGTGGTATAATTTGAAATTTATATAATTTTATTAGGAGATTAAAAAGGTAAACTTCGAAATTATCTTCGCAACCTCGCGATTATTGCCCACGTTGCCCATGGGaaa                                             <  2:870676/140‑1 (MQ=255)
                                                                                           tACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAAc                                           >  2:957025/1‑140 (MQ=255)
                                                                                           tACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAAc                                           >  1:1252256/1‑140 (MQ=255)
                                                                                           tACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAAc                                           >  1:788327/1‑140 (MQ=255)
                                                                                            aCAGAAAAACGTGACGCTTTCAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                               >  2:4736265/1‑119 (MQ=255)
                                                                                            aCAGAAAAACGTGACGCTTTCAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGcc                                                               <  1:4736265/119‑1 (MQ=255)
                                                                                                         actcTTTAAAAGAGTTTGTGTGTTATATTTTTAAAGTTATATAATTTTATTATGATATGAAAAATTTAAACTTCTAAATGATCTTCGCAAAATCGCTATTTTTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATc                             <  2:4674112/137‑1 (MQ=255)
                                                                                                         aCGCTTTTAACGAGTCTGGTGGTTCTTATGTGAAAGTTATCAAATTTTATTAGGAGATGAAAACGTGAAAATTCAAAATGCTCTTCGCAACATCGCTATTATTGCCCAGGTGGACCAGGGCAAAACGACTCTAGTCGATc                             <  2:4447568/140‑1 (MQ=255)
                                                                                                         aCGCTTTTAAAGAGGTTGTTTGTTATATTTTTAAAGTTATTTAATTTTATTAGGAGATCAAAAAGTTAAACTTCGAAATGATCTTCGCAACATCGCTATTTTTGCCCAGGTTGACATTGGGAAAACGACTCTAGTCGATc                             <  2:3357801/140‑1 (MQ=255)
                                                                                                               taaaaGAGGTTGTGTTTTATACTGTGAATGTTATAAATTTTTTTTAGGTGATTAAAAAGTAAAAGTTCTAATTGATCTCCGCAACATCGCTTTTTTTGCCCCGTTTCACCATGGAAAAACGACTCTAGTCGATCAGCttt                       <  1:5080945/138‑1 (MQ=255)
                                                                                                                      ggATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACAATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCa                >  1:4827874/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCTCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  2:1641621/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCac               >  2:6371987/1‑139 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  2:4287533/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  1:995741/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  1:3548325/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  1:2763895/1‑140 (MQ=255)
                                                                                                                       gATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAg               >  1:1993216/1‑140 (MQ=255)
                                                                                                                        ttttttgGTATATTTTTAATGTTATCTATTTTTTTTTGTTGATTAAAATGTTAAAGTTGTAATTGATCTGCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAgg              <  2:5243080/135‑1 (MQ=255)
                                                                                                                        tttggtgtTATAATGTTAAATTTTTTTTTTTTTTTTTGTTGATGAAAAATTGAAAGTTCGAAATTTTTTTCGAAACATCGGTTTTATTGCCCACTTTTACCATGGGAACACGACTCTAGTCGATCAGCTTTTACACCAgg              <  2:6662197/136‑1 (MQ=255)
                                                                                                                        ttgtgttTTATATTTTTAAATTTATTTATTTTTATTTGGATATTAAAAATTTAAATTTCTAAATGATGTTCGCAACTTCGCTTTTATTGCCCAGGTTGACCTGGGTAACACGACTCTAGTCGATCAGCTTTTACACCAgg              <  2:6439225/139‑1 (MQ=255)
                                                                                                                        ttgtgttGTATATTGTTAATTTTATAATTTTTTTTTAGGAGATTAAAATTTCAAACTTCGAAATTATCTTCCCAACATCGCTTTTTTTGCCCAGGTTCACCATGGCAACACGACTCTAGTCGATCAGCTTTTACACCAgg              <  1:972913/139‑1 (MQ=255)
                                                                                                                        ttgtgtgTTATATTGTTAATTTTATATATTTTTATTAGGAGATTAAAATGTCAAACTTCCAAATGATATTCCCAACCTCGCTTTTATGCCCCACGTGCACCTGGGCAACACGACTCTAGTCGATCAGCTTTTACACCAgg              <  1:2360008/139‑1 (MQ=255)
                                                                                                                        tggggtgGTATAATTTTAAATTTTTATATTTTTATTTGTCGTTTAAAAAGTTAAACTTCGAACGGATCTTCGAAACCTCGCTTTTATTGCCCAGGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAgg              <  2:841031/136‑1 (MQ=255)
                                                                                                                        gggggtgGTATAAGTGTAAAGTTATCTAATTTTTTGAGCAGATTAAAACGTGAAACTGCGAATTTTTCTTCGAAACACCGCTATTCTGGCCCACGTTCACAATGGCAAAACGACTCTAGTCGATCAGCTTTTACACCAgg              <  1:630281/136‑1 (MQ=255)
                                                                                                                         tgtgtgGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTACTCAACATCGCGATTATTGACCACGTAGACCATGGGAAAACCACTCTAGTCGATCACCTTTTAAACCAGGc             >  2:6051823/1‑140 (MQ=255)
                                                                                                                              tgttataTTGTTAATGTTATCTAATTTTTTTAGGAGATTAAAAATTTAAACTTCTAAATGATGTTCCAAACATCGCTTTTTTTCCCCCCGTTGACCTTGGCAACACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGt         <  1:2177888/137‑1 (MQ=255)
                                                                                                                               gTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTAc       >  1:636676/1‑140 (MQ=255)
                                                                                                                               gTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTAc       >  1:1104022/1‑140 (MQ=255)
                                                                                                                                   taATGTTAAATTTATCTATTTTTATTAGTTTATGAAAAAGTTAAACTTCTAAATTATCTTCGAAACATCGCGTTTTTTGCCCACTTTCACCTTGGTAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGtt    <  2:2101073/140‑1 (MQ=255)
                                                                                                                                     aTGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTcc  >  1:145540/1‑140 (MQ=255)
                                                                                                                                     |                                                                                                                                            
AATACTGAAAACCGCTGCAAATGCAAATAATAGTAAAAGCCAATTCATTTTTTTCATATTTTCCATCCCCTTTTACGTCTTATCATAAGTTTACAGAAAAACGTGACGCTTTTAAAGAGGATGTGTGGTATAATGTGAAAGTTATCTAATTTTATTAGGAGATGAAAAAGTGAAACTTCGAAATGATCTTCGCAACATCGCGATTATTGCCCACGTTGACCATGGGAAAACGACTCTAGTCGATCAGCTTTTACACCAGGCTGGTACGTTCC  >  NZ_CP034943/1512416‑1512687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: