Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,514,854 T→G 12.0% intergenic (+58/+1) EO946_RS07915 → / ← EO946_RS07920 YlaH‑like family protein/YlaI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,514,8540TG12.0% 780.2 / 25.9 334intergenic (+58/+1)EO946_RS07915/EO946_RS07920YlaH‑like family protein/YlaI family protein
Reads supporting (aligned to +/- strand):  ref base T (83/197);  new base G (51/3);  total (134/200)
Fisher's exact test for biased strand distribution p-value = 4.92e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 337 total aligned reads displayed.

TTGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAG  >  NZ_CP034943/1514716‑1514992
                                                                                                                                          |                                                                                                                                            
ttGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCtt                                                                                                                                             >  2:5303858/1‑140 (MQ=255)
 tGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCttg                                                                                                                                            >  2:6431095/1‑139 (MQ=255)
  gATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTa                                                                                                                                           >  2:6789826/1‑140 (MQ=255)
  gATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTa                                                                                                                                           >  1:2783954/1‑140 (MQ=255)
  gATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTa                                                                                                                                           >  1:2468402/1‑140 (MQ=255)
        cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                                     >  1:4765943/1‑140 (MQ=255)
        cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAtttttt                                                                                                                                     >  2:1133139/1‑140 (MQ=255)
        cgctgcgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTGTAtttttt                                                                                                                                     >  2:6376457/1‑140 (MQ=255)
             cgctgATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGActt                                                                                                                               <  2:5641963/140‑1 (MQ=255)
                  aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGGCTTGTTTTGTGGTTTGTCTGGTAAAGCGGCCGCGTGATTTTTTGAcgttttgt                                                                                                                          >  1:3682811/1‑138 (MQ=255)
                  aTTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCGGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGGTATTGTTTGAgtttttgt                                                                                                                          >  2:5394578/1‑138 (MQ=255)
                          cATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTg                                                                                                                  <  2:1052284/140‑1 (MQ=255)
                             ttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTGCTGt                                                                                                              <  2:2097227/140‑1 (MQ=255)
                                cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGCGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                            <  1:992183/140‑1 (MQ=255)
                                cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                            <  2:2008134/140‑1 (MQ=255)
                                cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                            <  2:320259/140‑1 (MQ=255)
                                cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAAGCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTcc                                                                                                            <  1:6995919/140‑1 (MQ=255)
                                     ttCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGt                                                                                                       <  2:7645172/140‑1 (MQ=255)
                                     gtCGGCTGGATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGt                                                                                                       <  2:5868439/139‑1 (MQ=255)
                                            ttATCAGTCGAAAAAGGGACAGTCTGCAAAATGATAAAAAGACTCAGCTGCGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGt                                                                                               <  1:5883197/139‑1 (MQ=255)
                                            gTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGt                                                                                               <  1:7478939/140‑1 (MQ=255)
                                               tCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGaa                                                                                            <  2:6276400/140‑1 (MQ=255)
                                               tCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGTCTTTTTCTTTTTTTTTGTGTGCCGGGTGTAAAGTGaa                                                                                            >  1:1225873/1‑140 (MQ=255)
                                                       aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGGAGTTTGGTTTTTTTTGGTTGGCCCGGGGAAAATTGAATTTTACCg                                                                                    >  1:4105261/1‑140 (MQ=255)
                                                       aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCg                                                                                    >  2:1582433/1‑140 (MQ=255)
                                                       aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTGTTTTTTTTTCTGTGCCGGGTGTAAATTTAATTTTACCg                                                                                    >  1:3032903/1‑140 (MQ=255)
                                                             gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGTAAAGTGAATTTTACCGTTTTCa                                                                              >  2:31356/1‑140 (MQ=255)
                                                             gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTTTCTTTTTGTTTTTTTTTGTGTCCCGGGTGAAAAGTGAATTTTCCCGttttaa                                                                              >  1:6908228/1‑138 (MQ=255)
                                                             gACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTTTTGTATGGTAAAGCGGCCGCGTTAATTTTTGTCTTTTTCTTTTTTTTTGTGTCCCGGGTGAACAGTGAATTTTCCCGTTTTCa                                                                              >  1:6021005/1‑140 (MQ=255)
                                                             gACAGTCTGCAAAATCATAAAAGACTCAGCTGAAAGCTGAGTCTTTTTTGTGTTCTGTATGGTTAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTCTGCTGTCCCGGGTGAAAAGTAAATTTTCCCGTTTTCAGcc                                                                           >  2:495490/1‑140 (MQ=255)
                                                                aGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGGCTGTTTGTTTTTTTTTGTTTCCCGGGTGTAAAGTGTATTTTGCCGTTTTGAGcc                                                                           >  1:2343711/1‑140 (MQ=255)
                                                                 gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTGTGTTTTTGTTTTTTTTTCTTTGCCGGGTGAAAAATTGAATTTACCGGtttaagacg                                                                           >  1:1073671/1‑134 (MQ=255)
                                                                 gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTTTCTTTTTGTTTTTTTTTCTTTGCCGGGTGCAAAGTGTATTTTACCGTTTTCAGGCg                                                                          >  1:4277263/1‑140 (MQ=255)
                                                                 gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGGTATTTTTTTACTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAATGAATTTTGCCGGTTTCAGcag                                                                          >  1:3116109/1‑138 (MQ=255)
                                                                 gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGGTTTGTATGGTAAAGCGGCCGCGGTATTTTTTTACTTTTTGTTTTTTTTTGTGTACCGGGTGAAAAGTTAAATTTACCGTTTTAAGCCg                                                                          >  1:650392/1‑140 (MQ=255)
                                                                 gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTGTTGTATGGTAACGCGGCCGCTTTATTTTTTGTATTTTTGTTGTTTTTGCTGTACCGGGTGAAAAGTGGATTTTGCCGTTTTGAGCCg                                                                          >  2:2785903/1‑140 (MQ=255)
                                                                    tGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTTTTTTTTGACTTTTTGTTTTTTTTTGTTTCCCGGGTGAAAAGTTAATTTTACCGTTTTAAGCCggtg                                                                       >  1:1939899/1‑137 (MQ=255)
                                                                        aaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                                   <  2:7269638/140‑1 (MQ=255)
                                                                           tCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAGTTTTTCTTTTTTTTGGTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTATTCTGtt                                                                >  2:364298/1‑140 (MQ=255)
                                                                            cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                       <  1:3348471/116‑1 (MQ=255)
                                                                            cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTGGTGTTTTGTATGGTAAAGCGGCCGCTTGATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAACAGTGAATGTTCCCGTTTTCAGGCGTGCTTCTGttt                                                               >  2:6099487/1‑140 (MQ=255)
                                                                            cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTGTTTTGTGTGTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTTTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTGGGTCTGttt                                                               >  2:2649311/1‑140 (MQ=255)
                                                                              tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGGATGGTAAAGCGGCCGCTTTATTTTTTGACGTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTCGTCTGttttt                                                             >  2:567918/1‑140 (MQ=255)
                                                                                  aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                         <  1:1930099/140‑1 (MQ=255)
                                                                                  aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTGCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                         <  2:5492789/140‑1 (MQ=255)
                                                                                  aaGACGCCGCTGAGAAGCGGAGTCTTTTATTGTGTTGTGTAGGGTAACGCGGCCGCTTAATTTTTTGACTGTTTCTTTTTGTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                         <  2:5215005/140‑1 (MQ=255)
                                                                                          gCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCgg                                                 <  2:3667186/140‑1 (MQ=255)
                                                                                           cgcagaAGCTGAGTCTTTTTTTGTGTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                                <  2:4279118/137‑1 (MQ=255)
                                                                                           cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                                <  1:6660833/140‑1 (MQ=255)
                                                                                                aaGCTTAGTCATTTTATGTGATTTGTATGGTAAACCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTgg                                           <  1:1795817/140‑1 (MQ=255)
                                                                                                aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTGGTTTACCCGGTGAAAAGGTAATTTTGCCGTTTTCAGGCGTTCTTGTGTTTTTTGGGCAAAACGGTCGTgg                                           >  2:242285/1‑140 (MQ=255)
                                                                                                 aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGACAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                          <  2:1255551/140‑1 (MQ=255)
                                                                                                 aGCTGAGTCTTTTTTTGTGTATTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                          <  2:3971410/140‑1 (MQ=255)
                                                                                                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTTCTTTTTGTTTTTTTTTGTGTCCCCGGTGAAAAGTTAATTTTACCGTTTTAATCCGTTCTTGTGTTTTTTTGGCGATACGGTCGTGGCAt                                        >  1:1092125/1‑140 (MQ=255)
                                                                                                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTGTTTTTTTTTCTTTACCCGGTGTAAAATGAATTTTAACGTTTTGAGCCGGTGTTCTGTTTTTTTGGGAATACGGTGGTGGCAt                                        >  1:5106038/1‑140 (MQ=255)
                                                                                                     gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAGTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                      >  2:2729451/1‑140 (MQ=255)
                                                                                                     gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTc                                                                                       >  2:1464639/1‑91 (MQ=255)
                                                                                                     gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                      >  2:7379781/1‑140 (MQ=255)
                                                                                                        tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTTTTTTTTTTCTTTTTTTTTTTTTTTGTTTACCGGGTGAAAAGTTAATTTTACCGTTTTAAGACGTTATTGTTTTTTTTTTGCAATACGGTCGTTGGATTACTg                                   >  1:7122442/1‑140 (MQ=255)
                                                                                                        tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTTCTTTCCCGGGTTAAAAGTGAATTTTACCGTTTTAAGGCGTTCTTATTTTTTTTTGTCAATAAGGTAGTGGCATTGCTg                                   >  2:1376312/1‑140 (MQ=255)
                                                                                                        tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTGTATTTTTTTTGTTTTTTTTTTTTTTTGTTTCCCGGGTGAAAAATGAATTTTTCCGTTTTGAGACGTTCTTGTGTTTTTTTGTGAAACAGCTCGTGGCATTACTg                                   >  1:5839301/1‑140 (MQ=255)
                                                                                                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                  <  1:4184080/140‑1 (MQ=255)
                                                                                                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                  <  2:6138829/140‑1 (MQ=255)
                                                                                                          tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                 <  1:1038095/140‑1 (MQ=255)
                                                                                                          tttgtttgTGTTTTGTATGGTAACGCGGCCGCTTTATTTTGTGACTTTTTCTTTTTTTTGCTGGCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                 <  2:5360303/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATTGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                <  1:4643488/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAATGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                <  2:3395594/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                <  1:5081807/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                <  1:2550627/140‑1 (MQ=255)
                                                                                                           ttttttGTGTTTTGTATGCTAAAGCGGCCGCTTTATTTTGTGACTTTTTCTGTTTTTTGCTGTCCCGGGTGCAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                <  2:3268613/140‑1 (MQ=255)
                                                                                                            tttttGTGTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcac                               <  2:6648901/140‑1 (MQ=255)
                                                                                                            tttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcac                               <  2:2889593/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTAACGTTTTAAGCCGTTCTTCTGTTTTTTTGGCAATACGGTCGTGGCAATCCTGGcaca                              >  2:2191041/1‑140 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  1:6017257/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  2:4765943/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  1:5303858/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  2:6609046/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  1:5731971/140‑1 (MQ=255)
                                                                                                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                              <  1:7139040/140‑1 (MQ=255)
                                                                                                              tttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                             <  2:420859/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTTTTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:7581962/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:1554147/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:1450634/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:2118742/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  2:4614436/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  2:3702537/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:3388526/140‑1 (MQ=255)
                                                                                                               ttGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                            <  1:6450465/140‑1 (MQ=255)
                                                                                                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                           <  1:5439124/140‑1 (MQ=255)
                                                                                                                    ttttGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                       <  2:4105261/140‑1 (MQ=255)
                                                                                                                    ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTTAATTTTACCGTTTTCAGCCGTTCTTGTGTTTTTTGGGCAATACGGTCGTTGCATTCCTGGCACATAaatgt                       >  2:7156684/1‑140 (MQ=255)
                                                                                                                    ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                       <  1:849536/140‑1 (MQ=255)
                                                                                                                     tttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgta                      <  1:6834569/140‑1 (MQ=255)
                                                                                                                         tagggTAACGCGGCCGCTTTATTTTTTGCCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGa                  <  1:7192255/137‑1 (MQ=255)
                                                                                                                         tATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGa                  <  1:3555220/140‑1 (MQ=255)
                                                                                                                                 aGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTTCTGTACCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATACGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCgg          >  2:2797539/1‑140 (MQ=255)
                                                                                                                                 aGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTTCTGTACCCGGTGAAAAGTGAATTTTACCCGTTTGATCCGTTCTTCTGTTTTTTTGGCAAAACGGTCGTTGCATTACTGGCACATAAATGTATTGATTTGGCgg          >  2:2478008/1‑140 (MQ=255)
                                                                                                                                   cGGCCGCTTTATTTTTTTACTTTTTGGTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGGCGTTCTTCTTTTTTTTGTGCAATCCGGTCGTGGCATTCCTGGCACATATATGTAAGGATTGGGCGGtt        >  2:2997652/1‑140 (MQ=255)
                                                                                                                                   cGGCCGCGTTATTTTTTTACTTTTTGTTTTTTTGCTGTCCCGGGTGAAAAATTAATTTTGACGTTTTGAGCCGGTGTTGTGTTTTTTTGGGAATACGGTGGTGGCATTACAGGCCCATAAATGTATTGATTTGGGGGttt       >  1:4397033/1‑140 (MQ=255)
                                                                                                                                      ccGCTTGCTTTTTTGACTTTTTGTTTTTTTTGCTGTCCCGGGTGAAAAGTGACTTTTCCCGTTTTCAGCCGTTCTTGTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTTGCACATATATTTCTGGGTTGGGCGGTGTct     >  2:6989320/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                            
TTGATCGTCGCTGCGCTGATTTTAATCATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAG  >  NZ_CP034943/1514716‑1514992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: