Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,514,935 G→T 5.1% Q44K (CAA→AAA)  EO946_RS07920 ← YlaI family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,514,9350GT 5.1% 1049.5 / 12.5 350Q44K (CAA→AAA) EO946_RS07920YlaI family protein
Reads supporting (aligned to +/- strand):  ref base G (57/266);  new base T (25/2);  total (82/268)
Fisher's exact test for biased strand distribution p-value = 1.71e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

AAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCACCTCT  >  NZ_CP034943/1514797‑1515073
                                                                                                                                          |                                                                                                                                          
        aGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTCTTCTGTTTTTTGGGCAATCCg                                                                                                                                   >  2:7323839/1‑140 (MQ=255)
          cgcagaAGCTGAGTCTTTTTTTGTGTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                                                                                                                 <  2:4279118/137‑1 (MQ=255)
          cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGt                                                                                                                                 <  1:3411694/140‑1 (MQ=255)
               aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGGTATGTTTTGTCTGTTTGTTTTTTTTGGTGTGCCGGGTGAAAAGTGAATTTTACCGTTTTGAGCGGGGCTGCGGTTTTTTTGGCAATCCGGTCgggg                                                                                                                            >  1:2018339/1‑140 (MQ=255)
                aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                                                                                           <  2:3055307/140‑1 (MQ=255)
                aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                                                                                           <  1:6656437/140‑1 (MQ=255)
                  cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGTCTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTCCCCTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCAt                                                                                                                         >  2:4690294/1‑140 (MQ=255)
                    gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGAGTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                                                                                                       >  2:2729451/1‑140 (MQ=255)
                     aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                                                                                                                      <  2:3994015/140‑1 (MQ=255)
                       tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTGTTTTTTTTGCTTTACCGGGTGAAAATTTAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTTGGCAATACGGTAGTGGCATTCCTg                                                                                                                    >  2:2863433/1‑140 (MQ=255)
                       tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTGTTTTTTTTTCTTTCCCGGGTTAAAAGTGAATTTTACCGTTTTAAGGCGTTCTTATTTTTTTTTGTCAATAAGGTAGTGGCATTGCTg                                                                                                                    >  2:1376312/1‑140 (MQ=255)
                       tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                    <  2:537621/140‑1 (MQ=255)
                       tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                    <  1:1582433/140‑1 (MQ=255)
                        cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                   <  2:7223721/140‑1 (MQ=255)
                        cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTGTTGCTGGCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                   <  2:5617131/140‑1 (MQ=255)
                        cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGATTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                   <  1:6968851/140‑1 (MQ=255)
                         tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                                                                                  <  1:1038095/140‑1 (MQ=255)
                         tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGGTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                                                                                  <  1:7657358/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  2:1459471/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  1:7110162/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  2:3697396/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  2:3394754/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  1:4369126/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  1:1551239/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  2:5224734/140‑1 (MQ=255)
                          ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTGGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                                 <  2:2460022/140‑1 (MQ=255)
                           tttttGTGTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcac                                                                                                                <  2:6648901/140‑1 (MQ=255)
                            ttttGTGTTTTGTTTGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  1:5187195/140‑1 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTAACGTTTTAAGCCGTTCTTCTGTTTTTTTGGCAATACGGTCGTGGCAATCCTGGcaca                                                                                                               >  2:2191041/1‑140 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  1:5303858/140‑1 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  1:6017257/140‑1 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  1:7580846/140‑1 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTGTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  2:6829261/140‑1 (MQ=255)
                            ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTCTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                               <  1:2951498/140‑1 (MQ=255)
                             tttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                                                                              <  2:420859/140‑1 (MQ=255)
                             tttGTGTGTTGGATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTGTGCTGTCCCGGGTGAAAAGTGAAATGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                                                                              <  2:6179149/140‑1 (MQ=255)
                              ttGTGTTTTGTTTGGTAAAGCGGCGGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGTATTCCTGGCACata                                                                                                             <  1:573166/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:7312679/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:961738/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  2:6120149/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  2:4431785/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:1091978/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:6789826/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:1346773/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:2118742/140‑1 (MQ=255)
                              ttGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                             <  1:6450465/140‑1 (MQ=255)
                               tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                                                                                                            <  1:1899226/140‑1 (MQ=255)
                                 tgtTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTAAATTTTGTTTTTTTTTCTTTAACCGGTGAAAAATTAAATTTAAACTTTTAAAACGTTATTATGTTTTTTTTGCAAAACCGTAGTTGCAATACTGGcaaaaaaaa                                                                                                          >  1:2474282/1‑133 (MQ=255)
                                 tgtTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGTATTTTTCTTTTTTTTTGTGTACCGGGTGAAAAGTGAATTTTCCCTTTTTAGGCCGGTGTTTGGTTTGTTGGGGACTCCGGGGGGGGGATTGGTGGCACatctat                                                                                                          >  1:4234577/1‑140 (MQ=255)
                                   ttttGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                                                                                                        <  2:4105261/140‑1 (MQ=255)
                                   ttttGTATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                                                                                                        <  2:6486431/140‑1 (MQ=255)
                                   ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTCCCGGGTGAAAAATTAATTTTAACGTTTTCAGCCGTTCTTCTGTTTTTTGGGAAATACGGTCGTGGCATTGCTGGCACATAtatgt                                                                                                        >  2:5463563/1‑140 (MQ=255)
                                   ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                                                                                                        <  2:2044293/140‑1 (MQ=255)
                                   tattGTATGGTAAAGCGGCCGCTTTATTTGTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                                                                                                        <  1:7133580/138‑1 (MQ=255)
                                      tGTATGGTAAAGCGGCCGCTTTATTTTTTGACGTTTTCTTTTTTTTTCTTTCCCGGGTGAAAATTGAATTTTCCCGTTTTAAGCCGTTCTTATGTTTTTTGTGCAATCAGGTCGTGGCATTACTGGCACATAtatgtatg                                                                                                     >  2:6350091/1‑140 (MQ=255)
                                              aacgCGGCCGCTTTATTTTTTGACTTGTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGc                                                                                             <  1:3071344/137‑1 (MQ=255)
                                                aGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTCTGTACCGGGTGAAAAATGAATTTTAACGTTTTAAGCCGTTGTTCTGTTTTTTGGGCAATCCGGTCGTGGAATTCCTGGCACATATATTTATGGATTGGGCgg                                                                                           >  2:3664134/1‑140 (MQ=255)
                                                aGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATACGGTCGTTGCATTACTGGCACATATATTTATGGATTGGGCgg                                                                                           >  2:1853317/1‑140 (MQ=255)
                                                     ccGCTTTATTTTTAGACGTTTTCTTTTATTTTCTGTCACCTGTGAAAAGTGAATATTCCCCATTTAAGAGGTTGTTCTGTTTTTTTGGGAATCCGGTCGTGGCATTCCTTTCACATATATTGATGGAGTGGGGGGTTTCg                                                                                      >  2:4961035/1‑140 (MQ=255)
                                                                  tGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTcgcg                                                                         <  2:5959628/140‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGTATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  2:1073671/140‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                    <  2:114796/95‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                    >  1:114796/1‑95 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  1:3950132/140‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  2:3333563/140‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  1:7412922/140‑1 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGGCCCGTTTTCAGCCTTTCTTCTTTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACAGCTATGTATGGATTGGGCGGTTTAGAAGCCGTTTcgcgct                                                                       <  1:4633382/140‑3 (MQ=255)
                                                                    aCTTTTTCTTTTTTTTGCGGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  1:5836398/140‑1 (MQ=255)
                                                                    aCTTTGTCTTTTTTTTGCTGTCCCGGGTGATAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  1:3694706/140‑1 (MQ=255)
                                                                    aCTGTTTCTTTTTTTGGCGGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTATTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                       <  2:7470535/140‑1 (MQ=255)
                                                                     ctttttctttttTTTGCTGTCCCGGGTGAACAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCAGTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc                                                                      <  2:5319002/140‑1 (MQ=255)
                                                                      tttttctttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCa                                                                     <  1:5088385/140‑1 (MQ=255)
                                                                           ctttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTa                                                                <  2:2795268/140‑1 (MQ=255)
                                                                             tttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatc                                                              <  1:557774/140‑1 (MQ=255)
                                                                                        tCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCACTCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGGTTCGCGATCAGGGTATCATCATCAATTg                                                   >  2:2651883/1‑140 (MQ=255)
                                                                                             ggTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCa                                              >  2:2961925/1‑140 (MQ=255)
                                                                                             ggTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCa                                              >  1:5075609/1‑140 (MQ=255)
                                                                                               tGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAAt                                            >  1:5975408/1‑140 (MQ=255)
                                                                                                    aGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTAt                                       >  2:1617965/1‑140 (MQ=255)
                                                                                                    aGTGAATGTTCCCGTGTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTAt                                       <  2:4857441/140‑1 (MQ=255)
                                                                                                      tGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATAGGGCTGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTCTCATCATCAATTGTTTCAATTTTATCg                                     >  2:5333358/1‑140 (MQ=255)
                                                                                                             tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                              <  1:4903981/140‑1 (MQ=255)
                                                                                                             tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                              <  2:5827031/140‑1 (MQ=255)
                                                                                                              cccGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGa                             <  1:3257880/140‑1 (MQ=255)
                                                                                                                      cAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGTTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGa                     <  2:6537789/140‑1 (MQ=255)
                                                                                                                        tccGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAcc                   <  2:3876840/139‑1 (MQ=255)
                                                                                                                        gCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAcc                   <  1:2592333/140‑1 (MQ=255)
                                                                                                                        gCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAcc                   <  1:1617965/140‑1 (MQ=255)
                                                                                                                         ccGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAccc                  <  1:2729451/140‑1 (MQ=255)
                                                                                                                         ccGGTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAccc                  <  1:6376457/140‑1 (MQ=255)
                                                                                                                          cGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCt                 <  2:2343711/140‑1 (MQ=255)
                                                                                                                           gTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtc                <  2:1939899/140‑1 (MQ=255)
                                                                                                                            ttcttcTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtca               <  2:3142081/140‑1 (MQ=255)
                                                                                                                            ttcttcTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtca               >  2:7079744/1‑140 (MQ=255)
                                                                                                                             tcttctGTTGTTTGGGCACTCCGGGCGTGGCATTCCTGGCACAGATATGGATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGAAACATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtcat              <  1:2183863/140‑1 (MQ=255)
                                                                                                                             tcgtctGTTGTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtcat              <  1:7251236/137‑1 (MQ=255)
                                                                                                                              cttctGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtcatc             >  1:3516258/1‑140 (MQ=255)
                                                                                                                                     tttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAc      <  2:6021005/140‑1 (MQ=255)
                                                                                                                                      ttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAcc     <  1:4690294/140‑1 (MQ=255)
                                                                                                                                      ttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAcc     <  2:3493993/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                          
AAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCACCTCT  >  NZ_CP034943/1514797‑1515073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: