Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,582,930 C→A 9.9% H41N (CAT→AAT)  ylyA → sporulation‑related RNA polymerase‑binding protein YlyA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,582,9300CA 9.9% 934.3 / 16.2 398H41N (CAT→AAT) ylyAsporulation‑related RNA polymerase‑binding protein YlyA
Reads supporting (aligned to +/- strand):  ref base C (204/139);  new base A (3/52);  total (207/191)
Fisher's exact test for biased strand distribution p-value = 2.64e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 437 total aligned reads displayed.

AGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCTTGCTGT  >  NZ_CP034943/1582791‑1583069
                                                                                                                                           |                                                                                                                                           
agagCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCaatcaatc                                                                                                                                             >  1:5012991/1‑140 (MQ=255)
agagCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCaatcaatc                                                                                                                                             >  1:1741288/1‑140 (MQ=255)
agagCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCaatcaatc                                                                                                                                             >  1:1939115/1‑140 (MQ=255)
  agCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTATTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCAt                                                                                                                                           <  1:864275/140‑1 (MQ=255)
   gCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATa                                                                                                                                          <  1:6380787/140‑1 (MQ=255)
     aGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATaaa                                                                                                                                        >  2:6233260/1‑140 (MQ=255)
       gAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATaaaaa                                                                                                                                      >  2:7316028/1‑138 (MQ=255)
            gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAATCCCGCTCGCAGCAATCAATAATAAACAAagag                                                                                                                                 >  2:4937840/1‑140 (MQ=255)
            gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACaaaaac                                                                                                                                 >  1:4503612/1‑139 (MQ=255)
            gtgATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAg                                                                                                                                 >  2:2868455/1‑140 (MQ=255)
                  aatgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCAGCAAGCAGCAATCAATCATAAACAAAAAGCAActc                                                                                                                           >  2:870232/1‑140 (MQ=255)
                  aatgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTAAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAActc                                                                                                                           >  1:6045741/1‑140 (MQ=255)
                    tgaatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTg                                                                                                                         >  2:5535305/1‑140 (MQ=255)
                      aatgaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGAt                                                                                                                       >  2:4809887/1‑140 (MQ=255)
                      aatgaTAAACTGACCGAAATATATACGGAACTTCTTCTGACTAAAGAATAATTACAGTCGAGTTTATTTGAGTATTCTTGCTTTCAGGCTTCAACACGCCCGAAAGCAGAAATAAATCATAAACAAAAAGCAACTCTGAt                                                                                                                       <  2:6789619/140‑1 (MQ=255)
                         gctCAACTCACCGCAATATTCACGTAACTTATTCTGCCTAACTAAGAATTACAGTCGAGATAATTTGATTATTCTTGCTTTCAGGCTTAAACAAGCCCGCAAGCACAAATAAATCATAAAAAAAAAGCAACTCTGATCTa                                                                                                                    <  1:1025750/138‑1 (MQ=255)
                         gaTCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTa                                                                                                                    >  2:7062568/1‑140 (MQ=255)
                          aacAACTGACCGCAATATATCGGGAACTTCTTCTGACCAACGAAGAATTACAGTCGCTATTATTTGAGTTTTCTTGCTTTCTGGCTTCAACACGCCCGCACGCAGCAATCAATCAAAAACAAAAAGCAACTCTGATCTAt                                                                                                                   <  1:7505457/138‑1 (MQ=255)
                          aaaaaCTCACCGAAATATATACGGAACTTCTTCTTACTAAAGAATAATTACATTCTAGTTTATTTGAGTATTCTTGCTTACCGGATTCAACACGCCCGCAAGCAGCAATCAATAATAAAAAAACAGCAACTCTGATCTAt                                                                                                                   <  1:7592843/137‑1 (MQ=255)
                          aTCAACTGACCGCAATATATCCGGAACTTCTTCTGACGAAACAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                                   <  2:2154313/140‑1 (MQ=255)
                          aTCAACTGACCGCAATATATACGTAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTTTTTTAGTATTCTTGCTTTCAGGCTTAAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAt                                                                                                                   <  2:1286049/140‑1 (MQ=255)
                            cAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATca                                                                                                                 >  2:412066/1‑140 (MQ=255)
                            cAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATca                                                                                                                 >  2:7582004/1‑140 (MQ=255)
                            aaaCTGACCTCAATATATCCTGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTTTTCTTGCTTTAAGGCTTCACCAAGCCCGCAAGATGCAATCAATCATAAACAAAAAGCAACTCTGATCTATca                                                                                                                 <  2:5011948/139‑1 (MQ=255)
                              aCTGACCGAAATATATACCGAACTCCTACTGACTAAATAACAATTACAGTCGAGATTATTTTAGGATTCTTGCTTTCAGGCTTCAACACGCCCGCACCCAGCAATCAATAAAAAACAAAAAGCAACTCTGATCTATcaca                                                                                                               <  1:6655076/140‑1 (MQ=255)
                                  aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                           >  1:7037727/1‑140 (MQ=255)
                                  aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                           >  1:140797/1‑140 (MQ=255)
                                  aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                           >  1:5213262/1‑140 (MQ=255)
                                  aCCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTaa                                                                                                           >  1:2515159/1‑140 (MQ=255)
                                    cGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaag                                                                                                         >  1:4673040/1‑140 (MQ=255)
                                      cAATATATACGGAACTTCTTCTGACGAAAGAATAATTACTGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:7448567/140‑1 (MQ=255)
                                      cAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACATTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGAAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:1378111/140‑1 (MQ=255)
                                      cAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGATTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGAAATCAATAATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:1982114/140‑1 (MQ=255)
                                      cAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:5570067/140‑1 (MQ=255)
                                      cAATATATACGCAACTTCTTCTGACGAAAGAATAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:3406845/140‑1 (MQ=255)
                                       aaTATATCGGTAACTTCTACTGACTAAAGAATAATTACTGTCTATTTTATTTGTGTTTTCTTGATTTCAGGCTTAAACACGCCCGAAAGCAGCAAAAAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAaagaag                                                                                                      <  1:6805846/140‑1 (MQ=255)
                                       aaTATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaag                                                                                                      <  2:3965091/140‑1 (MQ=255)
                                       aaTATAGCCGGAAATTCTTCTGAGGAAAGAAGAATTACAGTCGTGTTTATTTGAGTATTCTTGGTTTCAGGCTTAAACAAGCCCGCAAGCAGCAATAAATATTAAACAAAAAGCAACTCTGATCTATCACATTAaagaag                                                                                                      <  2:4470915/140‑1 (MQ=255)
                                            ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTCTTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                                 >  2:2502029/1‑140 (MQ=255)
                                            ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                                 >  1:5536026/1‑140 (MQ=255)
                                            ataCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCtt                                                                                                 >  2:2739194/1‑140 (MQ=255)
                                             taCGGAACTTCTTCTTACTAAAGAATAATTACAGTCGTTTTTTTTTTATTTTTCTTGATTTCAGGCTTCAACACCCCCGCACGCAGCAATCAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTc                                                                                                <  1:539628/140‑1 (MQ=255)
                                              aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                               >  2:1827222/1‑140 (MQ=255)
                                              aCGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCa                                                                                               >  2:4597719/1‑140 (MQ=255)
                                                ggAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATAATTTGAGTATTATTGCTTTCAGGCTTCAACAAGCCCGAAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATATATCACATTAAAGAAGAGCTTCaat                                                                                             >  1:3090064/1‑139 (MQ=255)
                                                ggAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGAGTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAg                                                                                             >  1:3608719/1‑140 (MQ=255)
                                                 gaccttcttCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAaagaa                                                                                                       <  2:429157/126‑1 (MQ=255)
                                                 gAACTTCTTCTGACGAAAGAAGCATTACATTCGAGATTATTTGAGTATTCTTGCTTTCATGCTTCAACAAGACCGCAAGCAGCAATCAATCATAAACAAAAAGAAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                            >  1:4078656/1‑140 (MQ=255)
                                                 gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCAGATTAAAGAAGAGCTTCAAGa                                                                                            >  1:6906339/1‑140 (MQ=255)
                                                 gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                            >  1:5965877/1‑140 (MQ=255)
                                                 gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                            >  2:2344601/1‑140 (MQ=255)
                                                 gAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGa                                                                                            >  1:2190244/1‑140 (MQ=255)
                                                   aattcttcTGACGAAAGAATAATTACAGTCGAGATTATTTTAGTATTCTTGCTTTCAGGCTTCAAAAAGCCCGCAAGCAGCAAACAATCATAAACAAAAAGCAACTCATATATATAACAATAAAGAAAAGCTTAAAGACg                                                                                          >  2:5722224/3‑140 (MQ=255)
                                                   aCTTCTTCTGACGAAAGAAGAATTACAGTCGAGATATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAACAAGAGCTTCAAGACGt                                                                                         >  2:3066027/1‑140 (MQ=255)
                                                    cttcttCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGt                                                                                         >  2:375704/1‑140 (MQ=255)
                                                     ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGtt                                                                                        >  2:6946048/1‑140 (MQ=255)
                                                     ttcttcTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATGAAAGAAGAGCTTCCAGACttt                                                                                        >  2:5341750/1‑140 (MQ=255)
                                                       cttctGACGAAAGAAGAATTACCGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTtct                                                                                      >  2:6462459/1‑140 (MQ=255)
                                                         tctGACCTAACAATAATTACCGTCTAGATTATTTGAGTATTCTTGATGTCAGGTTTCAACACGCCCGCACGAAGAAATCAATAATAAAAAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTctcc                                                                                    <  1:474669/140‑1 (MQ=255)
                                                            gACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCg                                                                                 >  2:7650692/1‑140 (MQ=255)
                                                            gACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCg                                                                                 >  1:2714358/1‑140 (MQ=255)
                                                              ccaaaGAAGATTTACTTGCTAGTTTATTTGATTATTCTTGTTTTCAGGCTTCAACAAGCCCGAAAGCAGCAATCAATAATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                               <  1:2945236/138‑1 (MQ=255)
                                                              cGAAAGAAGAATACCACTCCCGATTATTTGAGTATTGTTGCTATCAGGCTCAAACCACCCCGCAAGCAGCAAACACTAATAAACAAAAAGCAACGCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCa                                                                               <  1:6610788/140‑1 (MQ=255)
                                                                 aagaagAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATTACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTg                                                                            >  1:4936241/1‑140 (MQ=255)
                                                                  agaagaATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGt                                                                           >  2:320624/1‑140 (MQ=255)
                                                                     acaaTTACATTCCAGATTTTTTGATTATTCTTGGTTACAGGCTACAACACCCCCGCACGCACCAATAAATAATAAACAAAACGCAACTCTGATCTATCACATTAAAGAATAGCTTCAAGACGTTCTCCTCGCATTGTCCa                                                                        <  1:965728/138‑1 (MQ=255)
                                                                                  agtttttttGATTTTTGTTGATTTCTGGATTCAACACGCCCGCCAGCAGCAATAAATAATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACgg                                                           <  1:1538924/134‑1 (MQ=255)
                                                                                    tttattTGATTATTCTTGCTTTCCGGCTTCAACAAGCCCGCACGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                         <  1:1644106/139‑1 (MQ=255)
                                                                                    attattTGAGTATTCTTGCTTTCAGTCTTCAACAAGCCCGCAACCAGAAATCAATCATAAACAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                         <  1:113584/140‑1 (MQ=255)
                                                                                    attattTGAGTATGCTTGCTTTAAGGCTTCAACAAGCCCGCAAGCAGCAATCACCCAGAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTa                                                         <  2:3490519/140‑1 (MQ=255)
                                                                                           gAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCgg                                                  <  2:6605704/140‑1 (MQ=255)
                                                                                           gAGTATTCTTGCTTTCAGGATACAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCgg                                                  <  2:3285713/140‑1 (MQ=255)
                                                                                              tATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATa                                               >  2:7140458/1‑140 (MQ=255)
                                                                                              tATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATa                                               >  1:3704278/1‑140 (MQ=255)
                                                                                               aTTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATAc                                              <  1:2787997/140‑1 (MQ=255)
                                                                                               aTTCTTGCTTTAAGGCTTCAACAAGCCCGCAAGCAGCAATCAATAATAAAAAAAACGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATAc                                              <  1:1312488/140‑1 (MQ=255)
                                                                                                ttCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACt                                             >  1:3910935/1‑140 (MQ=255)
                                                                                                ttCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACt                                             >  1:6519895/1‑140 (MQ=255)
                                                                                                  cTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGc                                           >  2:4889984/1‑140 (MQ=255)
                                                                                                    tGCTTTAAGGCTCCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGGTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCga                                         <  2:163437/140‑1 (MQ=255)
                                                                                                      cTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCgaag                                       >  1:3477710/1‑140 (MQ=255)
                                                                                                      cTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCgaag                                       >  2:6845240/1‑140 (MQ=255)
                                                                                                        ttCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGaa                                                                <  2:4423798/113‑1 (MQ=255)
                                                                                                        ttCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGaa                                                                >  1:4423798/1‑113 (MQ=255)
                                                                                                           aGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACt                                  >  2:5929106/1‑140 (MQ=255)
                                                                                                             gCTTCAACAAGCCCGCAAGAAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgg                                <  2:6038937/140‑1 (MQ=255)
                                                                                                               ttCACCACGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTAGCACATTAAAGACGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                              <  1:402548/140‑1 (MQ=255)
                                                                                                               ttCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTgggg                              <  2:2596849/140‑1 (MQ=255)
                                                                                                                       gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCATGACGTTCTCCTCGCATTGTCCAAAATCGAAAACCGTACATACGGATACTGCGAAGAAACTGGGGCTCCCAtg                      >  2:523783/1‑139 (MQ=255)
                                                                                                                       gCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCAtt                      >  2:1107651/1‑140 (MQ=255)
                                                                                                                            cAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCt                 >  1:5739237/1‑140 (MQ=255)
                                                                                                                            cAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTAAATTCGGCTACTGCGAAGAAACTGGGGCTCCCATTCACCt                 >  1:6236876/1‑140 (MQ=255)
                                                                                                                             aagacgcaATAAATCATAAAAAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGGTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCtt                <  1:3253744/135‑1 (MQ=255)
                                                                                                                             aaGCAGCAATCAATAATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCtt                <  2:3841622/140‑1 (MQ=255)
                                                                                                                                  gcaATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGGTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaaa           <  2:2219808/140‑1 (MQ=255)
                                                                                                                                  ccaatcaatAATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCaaa           <  2:2070038/139‑1 (MQ=255)
                                                                                                                                   caatcaatCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAg          >  2:1520017/1‑140 (MQ=255)
                                                                                                                                     atcaatcaTACACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCt        >  1:7101376/1‑140 (MQ=255)
                                                                                                                                       caatcaTAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCTtg      <  1:4204104/140‑1 (MQ=255)
                                                                                                                                       aaatcaTAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCACAGCTtg      <  1:2646962/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
AGAGCAGGAGTTGTGATGAATGAATGATCAACTGACCGCAATATATACGGAACTTCTTCTGACGAAAGAAGAATTACAGTCGAGATTATTTGAGTATTCTTGCTTTCAGGCTTCAACAAGCCCGCAAGCAGCAATCAATCATAAACAAAAAGCAACTCTGATCTATCACATTAAAGAAGAGCTTCAAGACGTTCTCCTCGCATTGTCCAAAATCGAAAACGGTACATTCGGATACTGCGAAGAAACTGGGGCTCCCATTCCCCTTTCAAAGCTTGCTGT  >  NZ_CP034943/1582791‑1583069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: