Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,591,355 T→G 11.1% W360G (TGG→GGG)  carB → carbamoyl‑phosphate synthase (glutamine‑hydrolyzing) large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,591,3550TG11.1% 1112.3 / 27.4 469W360G (TGG→GGG) carBcarbamoyl‑phosphate synthase (glutamine‑hydrolyzing) large subunit
Reads supporting (aligned to +/- strand):  ref base T (203/194);  new base G (2/70);  total (205/264)
Fisher's exact test for biased strand distribution p-value = 2.82e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 472 total aligned reads displayed.

ACAGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCGGATGTGTA  >  NZ_CP034943/1591217‑1591491
                                                                                                                                          |                                                                                                                                        
aCAGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTg                                                                                                                                         <  2:3939385/140‑1 (MQ=255)
 cAGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTgg                                                                                                                                        >  2:1279960/1‑140 (MQ=255)
  atgATATCCGATGGCAAAGCTCGCTGCTAACATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACACGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGc                                                                                                                                       <  2:4283565/138‑1 (MQ=255)
  aGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGATAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCAAAAATTCCGCGCTGGc                                                                                                                                       <  2:1587086/140‑1 (MQ=255)
  aGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGc                                                                                                                                       <  2:4018273/140‑1 (MQ=255)
          cGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  1:6455300/140‑1 (MQ=255)
          cGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  1:4332004/140‑1 (MQ=255)
          cGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCATTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  1:3807939/140‑1 (MQ=255)
          cGATGGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  1:3972290/140‑1 (MQ=255)
           gATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGTCTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  2:6092360/139‑1 (MQ=255)
           gATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               >  1:4451291/1‑139 (MQ=255)
            aTTGCAAAGCTCGCTGCTAAAATTGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATaa                                                                                                                             >  2:4779371/1‑140 (MQ=255)
            aTTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATaa                                                                                                                             >  2:4563253/1‑140 (MQ=255)
              tGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCGGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGt                                                                                                                           >  1:4631601/1‑140 (MQ=255)
              tGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGt                                                                                                                           >  2:1373503/1‑140 (MQ=255)
              tGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGt                                                                                                                           >  2:7589984/1‑140 (MQ=255)
              tGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGt                                                                                                                           >  1:3390308/1‑140 (MQ=255)
                  aaGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGa                                                                                                                       >  2:3608981/1‑140 (MQ=255)
                        gctgctAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGc                                                                                                                 <  1:2146887/140‑1 (MQ=255)
                         ctgctAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCa                                                                                                                <  1:3479131/140‑1 (MQ=255)
                                    gCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCATACAGAACGCt                                                                                                     >  1:3864853/1‑140 (MQ=255)
                                    gCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCt                                                                                                     >  2:5641857/1‑140 (MQ=255)
                                      tGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCg                                                                                                   >  1:2709657/1‑140 (MQ=255)
                                      tGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCg                                                                                                   >  1:7167982/1‑140 (MQ=255)
                                      tGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCg                                                                                                   >  1:1947375/1‑140 (MQ=255)
                                           gCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACg                                                                                              <  1:1279960/140‑1 (MQ=255)
                                                tCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAAt                                                                                         >  2:2509580/1‑140 (MQ=255)
                                                 cATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATg                                                                                        <  2:3873738/140‑1 (MQ=255)
                                                   ttAGTTGAAATGATGAACCCGGTGACAGGAAAAACATATGCACCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAATTTTGAATCAGAAAACAGAAAGCTCGGCACGAAAATGaa                                                                                      <  2:1687803/140‑1 (MQ=255)
                                                      gATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAt                                                                                                                               <  2:6252973/96‑1 (MQ=255)
                                                       aTGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCg                                                                                  >  2:21930/1‑140 (MQ=255)
                                                           aaTGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACgg                                                                              >  2:5259788/1‑140 (MQ=255)
                                                           aaTGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACgg                                                                              >  2:5151019/1‑140 (MQ=255)
                                                             ttatgaACCCGGGGACAGGAAAAACATCTGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGCCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATCAAAGCGACGGga                                                                            <  2:1134385/138‑1 (MQ=255)
                                                             tgatgaACCCGGTGACAGGAAAAACATCTGCCGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAACCAGAAAGCTCGGCCCGCAAATGAAAGCGACGGga                                                                            <  2:5929702/140‑1 (MQ=255)
                                                               atgaACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGgaga                                                                          <  2:3452475/140‑1 (MQ=255)
                                                                  aaCCCGGTGACAGGAAAAACTTATCCAGCTTTTCAACCGGCTCTGGACTCCGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATACGTTTTAATCACCAAACAGAAAGCGCGGCACGCAAATGAAAGCGACGGGAGAGGt                                                                       <  1:3021171/140‑1 (MQ=255)
                                                                   aCCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTc                                                                      >  1:7559462/1‑140 (MQ=255)
                                                                    cccGGTGACCGGAAAAACATCTCCAGCATTTGACCCGGCTCTTGACGACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGAGAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCa                                                                     <  2:2301125/140‑1 (MQ=255)
                                                                    cccGGTCACAGGAAAAACATATGCACCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATACGTTTGAATCCGCAAACACAAAGCTCGGAACGCAAATGAAAGCGACGGGAGAGGTCa                                                                     <  1:144311/140‑1 (MQ=255)
                                                                         tGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCTGCACACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCg                                                                >  1:7479216/1‑140 (MQ=255)
                                                                         tGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCg                                                                <  2:2103788/140‑1 (MQ=255)
                                                                                 aaaaCATATGCAGCATTTTAACGGGCTCTTTACTACGTCTTATCCAAAATTCCGCGCGGGCCGTTTTATAAGTTTGAATCAGCAAACATAAACCTCGGCACGAAAATCAAAGCGACGGGAGAGGTCATGGCGATTGGCCg                                                        <  1:6039185/140‑1 (MQ=255)
                                                                                   accATATGCCGCATTTGAACCGGCTCTTGACCACGTCTTATCCAAACTTCCGCGGTGGCCGTTTGATAAGTTTGAATCAGCACACAGAACGCGCGGCACGCAAATTAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  1:138146/138‑1 (MQ=255)
                                                                                   aaCATATGCAGCATTTTAACCGGCTCTTGACTACGTCGTATCAAAAATTCCGCGCGGGCCGTTTTATAAGTTTGAATCAGCAAACAGAAAGCTCGGCCCGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  2:4889904/140‑1 (MQ=255)
                                                                                   aaCATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAAACAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  2:1729866/140‑1 (MQ=255)
                                                                                   aaCATATCCAGCATTTGACCCGGCTCTTGCCTACGTCGTAACCAAATTTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  1:1600135/140‑1 (MQ=255)
                                                                                   aaCATAGCCCGCATTGGAACCGGCTCTGGACTACGTCGTACCCAAAATGCCGCGCGGGCCGTTTGATAAGTTTGAACCAGAAAACACAAAGCTCGCCACGCAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  1:6323753/140‑1 (MQ=255)
                                                                                          gccgcaTTTGAACCGGCTCTTGACTACGTCGTATCCAACTGTCCGCGCTGGCCGTTTGATAAGTTTGACTCAGCAACCAGAACGCTCGGCACGCAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGa                                               <  2:2810975/137‑1 (MQ=255)
                                                                                          gcagcaTTTGAACGGGCTGTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATTAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGa                                               <  2:3341394/140‑1 (MQ=255)
                                                                                          ccagcaTTTTAACGGGCTCTTGACTACGTCTTATCCAAAATCCCGCGCTGGCCGTTTGATAATTTTGAATCAGCAAACAGAAAGCTCGGAACGAAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGa                                               <  1:5020443/139‑1 (MQ=255)
                                                                                              caTTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAagag                                           >  2:849168/1‑140 (MQ=255)
                                                                                              caTTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAagag                                           >  1:6817778/1‑140 (MQ=255)
                                                                                               aTTTGAACCGGCTGTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAACTTTGAATCAGCAAACAGAAACCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGt                                          <  2:6303476/140‑1 (MQ=255)
                                                                                               aTTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGt                                          <  2:2908090/140‑1 (MQ=255)
                                                                                                tttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCACATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTc                                         >  1:4234187/1‑140 (MQ=255)
                                                                                                tttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCTATTGGCCGCACGCTTGAAGAGTc                                         >  1:5416503/1‑140 (MQ=255)
                                                                                                tttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTc                                         >  1:959054/1‑140 (MQ=255)
                                                                                                tttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTc                                         >  2:11994/1‑140 (MQ=255)
                                                                                                 tttaaCCGGCTCTTGACTCCGTCGTATCCAAAATTCCGCGCTGGCCGTTTTATAAGTTTGAATCAGCAACCATAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:1184646/137‑1 (MQ=255)
                                                                                                 ttGACCCGGCTCTGGACTACGTCGGAGCCAAAATTCCGCGCGGGCGGTTTGATAAGTTTCAATCAGCAAACAGAACGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:526180/140‑1 (MQ=255)
                                                                                                 ttGAACCGGGTCTTGACTCCGTCGTAACCACAATTCCGCGCTGGCCGTTTGATACGTTTGAAGCCGCAAACACAAAGCTCGGCACGAAAATCAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:2545360/140‑1 (MQ=255)
                                                                                                 ttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAACGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:1724850/140‑1 (MQ=255)
                                                                                                 ttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  2:7374168/140‑1 (MQ=255)
                                                                                                 ttGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAACCTCGGCACGCAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:2430782/140‑1 (MQ=255)
                                                                                                 ttGAACCGGCTCTGGACTCCGTCGTATCAAAAATTCCGCGCGGGCCGTTTTATAATTTTGAATCAGAAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        <  1:4937567/140‑1 (MQ=255)
                                                                                                       cGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTg                                  <  2:5434800/140‑1 (MQ=255)
                                                                                                       cGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCCCGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTg                                  <  2:4653139/140‑1 (MQ=255)
                                                                                                       cGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCGGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTg                                  <  2:7169667/140‑1 (MQ=255)
                                                                                                        ggCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGa                                 >  1:2246232/1‑140 (MQ=255)
                                                                                                        ggCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCGTGAAGAGTCATTGCTGa                                 >  2:6513213/1‑140 (MQ=255)
                                                                                                            ctTGACTACGGCGTATCCAAAATTCCGCGCGGGCGGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGCAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGc                             <  2:1056192/140‑1 (MQ=255)
                                                                                                                  tACGTCGTATCAAAATTTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACCCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCg                       <  1:3079992/140‑1 (MQ=255)
                                                                                                                   aggtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGt                      >  1:20964/3‑140 (MQ=255)
                                                                                                                   aCGTCGTATCCAAAATTCCGCGCGGGCCGTTTTATAAGTTTCAATCAGCAAACAGAAACCTCGGCACGCAAATGAACGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGt                      <  1:6093480/140‑1 (MQ=255)
                                                                                                                    cgtcgtCTCAAAAATGCCGCGCTGGCCGTTTTTTAATTTTGAATCAGCAAACATAATTCTCGTCACGAAAATCAAAGCGAGGGGAGAGGTCATGGCGATTGGCCTCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     <  1:5682696/140‑1 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:1117493/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:3191727/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:3603151/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:2297533/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:4865718/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:6758292/1‑140 (MQ=255)
                                                                                                                    cgtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGtt                     >  1:5161673/1‑140 (MQ=255)
                                                                                                                     gtcgtATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTc                    >  2:3730978/1‑140 (MQ=255)
                                                                                                                       cgtCTCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCAc                  >  2:7425078/1‑140 (MQ=255)
                                                                                                                       cgtATCCAAAATTCCGCGCTGGCCGTTTGAGAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGCGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCAc                  >  2:2584850/1‑140 (MQ=255)
                                                                                                                         tATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCa                                                      <  2:4359488/102‑1 (MQ=255)
                                                                                                                         tATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTg                >  2:701945/1‑140 (MQ=255)
                                                                                                                         tATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTg                >  2:6691037/1‑140 (MQ=255)
                                                                                                                         tATCCAAAATTCCGCGCTGGCCGTTTGATAAGTGTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTg                >  1:7349518/1‑140 (MQ=255)
                                                                                                                         tATCCAAAATTCCGCGCTGGCCGGTTGATAAGGTTGAAGCAGCAAACAGAACGCTCGGCACGCAAATGAATGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGTGTCCTTGCTGAAGGCTGTGGGTTCACTg                >  2:3034422/1‑140 (MQ=255)
                                                                                                                             cAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAg            <  2:1875933/140‑1 (MQ=255)
                                                                                                                             cAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAg            <  2:2732884/140‑1 (MQ=255)
                                                                                                                             cAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAAGGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAg            <  1:7055868/140‑1 (MQ=255)
                                                                                                                                aaTTCCGCGCTGGCCTTTTGATAAGTTTGAATCACCAAACACAACGCTCGGCACGCAAATCAACGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCgg         <  1:1003392/140‑1 (MQ=255)
                                                                                                                                aaTTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCa                                        >  1:2777767/1‑109 (MQ=255)
                                                                                                                                 aTTCCGCGCGGGCCGTTTGATACGTTTGAATCAGCAAACAGAACGCTCGGCACGCAAATCAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCGGa        <  1:4721028/140‑1 (MQ=255)
                                                                                                                                       cgcTGGCGGTTTGATACGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCGGATGTGTa  <  2:3154846/140‑1 (MQ=255)
                                                                                                                                       cgcTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGAGGGGAGAGGTCATGGCGATTGGCCGCAGGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTCGAAGCGGATGTGTa  >  1:805326/1‑140 (MQ=255)
                                                                                                                                       cgcTGGCCGTTTGATAAGTTTGAAGCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCGGaggggga  >  1:1792675/1‑134 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ACAGGATATCCGATTGCAAAGCTCGCTGCTAAAATCGCTGTCGGCCTTTCATTAGATGAAATGATGAACCCGGTGACAGGAAAAACATATGCAGCATTTGAACCGGCTCTTGACTACGTCGTATCCAAAATTCCGCGCTGGCCGTTTGATAAGTTTGAATCAGCAAACAGAAAGCTCGGCACGCAAATGAAAGCGACGGGAGAGGTCATGGCGATTGGCCGCACGCTTGAAGAGTCATTGCTGAAGGCTGTGCGTTCACTGGAAGCGGATGTGTA  >  NZ_CP034943/1591217‑1591491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: