Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,642,832 C→T 100% P128L (CCT→CTT)  rimM → ribosome maturation factor RimM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,642,8320CT99.2% 1146.7 / ‑3.1 312P128L (CCT→CTT) rimMribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base T (171/138);  total (171/141)
Fisher's exact test for biased strand distribution p-value = 9.12e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 312 total aligned reads displayed.

CGCAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCCTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTAATAGA  >  NZ_CP034943/1642698‑1642967
                                                                                                                                      |                                                                                                                                       
cGCAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAATACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAg                                                                                                                                    >  2:375710/1‑140 (MQ=255)
cGCAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAg                                                                                                                                    <  2:6208801/140‑1 (MQ=255)
  cAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCt                                                                                                                                  >  1:6233316/1‑140 (MQ=255)
  cAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCt                                                                                                                                  >  1:3845062/1‑140 (MQ=255)
          aaaGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGt                                                                                                                          <  1:3191420/140‑1 (MQ=255)
               gCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTggg                                                                                                                     >  1:3907743/1‑140 (MQ=255)
                ccTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTCATGACGTTTGGGt                                                                                                                    >  1:2129046/1‑140 (MQ=255)
                ccTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGt                                                                                                                    >  2:2773739/1‑140 (MQ=255)
                 cTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGtt                                                                                                                   >  2:3902218/1‑140 (MQ=255)
                          aTTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGa                                                                                                          >  2:7647712/1‑140 (MQ=255)
                             tAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGaaaa                                                                                                       <  2:6233316/140‑1 (MQ=255)
                             tAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGaaaa                                                                                                       <  2:3561675/140‑1 (MQ=255)
                             tAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGaaaa                                                                                                       <  2:3845062/140‑1 (MQ=255)
                                   agCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaa                                                                                                 >  2:5862139/1‑140 (MQ=255)
                                   agCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaa                                                                                                 >  1:4550827/1‑140 (MQ=255)
                                   agCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaa                                                                                                 >  1:3008005/1‑140 (MQ=255)
                                   agCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaa                                                                                                 >  1:1178825/1‑140 (MQ=255)
                                    gCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaaa                                                                                                >  1:6847981/1‑140 (MQ=255)
                                    gCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaaa                                                                                                >  1:743968/1‑140 (MQ=255)
                                    gCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaaa                                                                                                >  2:5197703/1‑140 (MQ=255)
                                     cTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGaaaaa                                                                                               >  2:1393963/1‑140 (MQ=255)
                                        aaTGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGa                                                                                            <  1:6514821/140‑1 (MQ=255)
                                        aaTGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGa                                                                                            <  2:7401498/140‑1 (MQ=255)
                                        aaTGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGa                                                                                            <  2:1367948/140‑1 (MQ=255)
                                             gggAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCAc                                                                                       <  2:4179743/140‑1 (MQ=255)
                                             gggAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCAc                                                                                       <  2:7252803/140‑1 (MQ=255)
                                              ggAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACt                                                                                      >  2:1697992/1‑140 (MQ=255)
                                              ggAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACt                                                                                      >  2:6465319/1‑140 (MQ=255)
                                              ggAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACt                                                                                      >  2:505760/1‑140 (MQ=255)
                                               gagaATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTc                                                                                     <  2:7496643/140‑1 (MQ=255)
                                               gagaATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTc                                                                                     <  2:2404791/140‑1 (MQ=255)
                                               gagaATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTc                                                                                     <  2:2984355/140‑1 (MQ=255)
                                                  aaTTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAAt                                                                                                                               >  2:6080971/1‑95 (MQ=255)
                                                  aaTTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCAtt                                                                                  >  2:834743/1‑140 (MQ=255)
                                                  aaTTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCAtt                                                                                  >  1:3667278/1‑140 (MQ=255)
                                                     tttATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCt                                                                               <  2:6700035/140‑1 (MQ=255)
                                                     tttAGTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCt                                                                               <  2:3282891/140‑1 (MQ=255)
                                                     tgatgtgccATGAATTCTTTGGGTGCGACTTATTTACGCAGCACGTTCAAGCCAGGGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCt                                                                               <  2:3926969/133‑1 (MQ=255)
                                                              aTGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGaa                                                                      >  2:7071584/1‑140 (MQ=255)
                                                               tGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAAt                                                                     <  1:2510322/140‑1 (MQ=255)
                                                               tGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAAt                                                                     <  2:1081625/140‑1 (MQ=255)
                                                                 aaaTCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCa                                                                   <  1:3341838/140‑1 (MQ=255)
                                                                   aTCATGGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCACAGAAATTTTGACGCTGGGAGCTACTGACGGTTGGGTTCTCTGGCGACAAGGGAAAAAAGACGCTCTCAGTCCTTACCTTGAATCact                                                                 >  2:4946100/1‑138 (MQ=255)
                                                                    tCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTg                                                                <  1:6123433/140‑1 (MQ=255)
                                                                    tCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTg                                                                <  1:286595/140‑1 (MQ=255)
                                                                      aTTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGt                                                              <  2:2726975/140‑1 (MQ=255)
                                                                      aTTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGt                                                              <  1:3299164/140‑1 (MQ=255)
                                                                       ttGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTc                                                             <  2:2123440/140‑1 (MQ=255)
                                                                       ttGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTAAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTc                                                             <  1:3589775/140‑1 (MQ=255)
                                                                        tGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCa                                                            >  2:5602608/1‑140 (MQ=255)
                                                                         gggTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCaa                                                           <  1:2660918/140‑1 (MQ=255)
                                                                           gTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTc                                                                                     >  2:2627902/1‑112 (MQ=255)
                                                                            tGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAata                                                        >  1:1338522/1‑140 (MQ=255)
                                                                            tGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAata                                                        >  1:1858103/1‑140 (MQ=255)
                                                                              cGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAatata                                                      >  1:3486128/1‑140 (MQ=255)
                                                                              cGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAatata                                                      >  1:7231119/1‑140 (MQ=255)
                                                                                      ttACCGAAGACTGTGAACTCATCGGAAAGGTCAAAGTAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTGAAATATATCGATGTc                                              >  1:7383565/1‑140 (MQ=255)
                                                                                      ttACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTc                                              >  1:7083482/1‑140 (MQ=255)
                                                                                        aCCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGt                                                              <  2:5790091/122‑1 (MQ=255)
                                                                                           gAAGACGGTGAACTCATCGGAAAGGTCAAAGAACTGTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCga                                         <  1:6714230/140‑1 (MQ=255)
                                                                                           gAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCga                                         <  1:7301171/140‑1 (MQ=255)
                                                                                           gAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCga                                         <  1:3141782/140‑1 (MQ=255)
                                                                                           cacgccGGGGAACTCATCGGAAAGGTCAAAGAAATTGGGACGCTTGGAGCGACTGACGTTTGGGTTATCGGGCGAACAGGGAAAAAAGACGCACTCAGTCCGTACCTTGAATCAGTGGTCAAATATATCGATGTCAGCga                                         <  1:7465326/135‑1 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTAt                                                                                                                 >  1:5576478/1‑66 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGaaaa                                                                                                       >  1:4697571/1‑76 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGaaaa                                                                                                       <  2:4697571/76‑1 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  1:4270605/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  2:1649501/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  2:6858321/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  1:4799602/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  1:5062931/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  2:4347154/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  1:5250653/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaa                                       >  1:7601108/1‑140 (MQ=255)
                                                                                             aGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCAGTCCTTACATTGTCTCAGTGGTCATATATATCGATGTCCGCgaca                                       >  2:5326003/1‑138 (MQ=255)
                                                                                                   tGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCgaaaagaaaa                                 >  1:3400971/1‑140 (MQ=255)
                                                                                                         cATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCt                                                                               >  2:1878991/1‑88 (MQ=255)
                                                                                                         cATCGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTgaga                           >  2:124783/1‑140 (MQ=255)
                                                                                                            cGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTg                        >  2:2362635/1‑140 (MQ=255)
                                                                                                            cGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTg                        >  2:2575749/1‑140 (MQ=255)
                                                                                                            cGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTg                        <  2:1002923/140‑1 (MQ=255)
                                                                                                            cGGAAAGGTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTg                        <  2:6903012/140‑1 (MQ=255)
                                                                                                                  ggTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCa                  >  1:5967240/1‑140 (MQ=255)
                                                                                                                  ggTCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCa                  >  1:5465449/1‑140 (MQ=255)
                                                                                                                    tCAAAGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATg                >  2:5132435/1‑140 (MQ=255)
                                                                                                                      aaaGAAATTTTGACGCTTGGAGGTAATGACGTTTGGGTTATCGGGCGACAAGGGAAAAAAGACGCACTCAGTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGa              <  1:1124306/140‑1 (MQ=255)
                                                                                                                      aaaGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGa              <  1:904685/140‑1 (MQ=255)
                                                                                                                      aaaGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGa              <  1:817625/140‑1 (MQ=255)
                                                                                                                      aaaGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGa              >  1:1007337/1‑140 (MQ=255)
                                                                                                                      aaaGAAATTTTGACGCCTGGAGCCAATGACGTTTGGGTCATCGGACGAAAAGGAAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTTAAACATATCGATGTCAGGGAAAAGAAAATTGAGATTGAACTCATGGa              <  1:4089294/140‑1 (MQ=255)
                                                                                                                        aGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGACTCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAg            >  2:5501253/1‑140 (MQ=255)
                                                                                                                        aGAAATTTTGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAg            <  1:6998568/140‑1 (MQ=255)
                                                                                                                             ttttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCa                                                                   >  2:4858895/1‑80 (MQ=255)
                                                                                                                             ttttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTa       >  1:5554671/1‑140 (MQ=255)
                                                                                                                             ttttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTa       >  1:2019572/1‑140 (MQ=255)
                                                                                                                             ttttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTa       >  1:1585811/1‑140 (MQ=255)
                                                                                                                              tttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTaa      >  1:5796522/1‑140 (MQ=255)
                                                                                                                               ttGACGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTAAt     <  2:7471083/140‑1 (MQ=255)
                                                                                                                                  aCGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTAATAGa  <  1:7649849/140‑1 (MQ=255)
                                                                                                                                  aCGCTTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTAATAGa  <  1:4347154/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGCAATCATTAAAGTGCCTGAAGAAGATTTAGGAGAGCTGAATGAGGGAGAATTTTATTTCCATGAAATCATTGGGTGCGAAGTATTTACCGAAGACGGTGAACTCATCGGAAAGGTCAAAGAAATTTTGACGCCTGGAGCTAATGACGTTTGGGTTATCGGGCGAAAAGGGAAAAAAGACGCACTCATTCCTTACATTGAATCAGTGGTCAAATATATCGATGTCAGCGAAAAGAAAATTGAGATTGAACTCATGGAAGGATTAATAGA  >  NZ_CP034943/1642698‑1642967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: