Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 135,117 G→T 6.8% intergenic (+164/‑74) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943135,1170GT 6.8% 1630.7 / 23.4 699intergenic (+164/‑74)EO946_RS00715/rpsJalpha/beta hydrolase/30S ribosomal protein S10
Reads supporting (aligned to +/- strand):  ref base G (317/308);  new base T (70/2);  total (388/311)
Fisher's exact test for biased strand distribution p-value = 4.95e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 706 total aligned reads displayed.

ACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134979‑135256
                                                                                                                                           |                                                                                                                                           
acCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  1:986695/140‑1 (MQ=255)
acCAGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  1:4028285/140‑1 (MQ=255)
  cTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCa                                                                                                                                          <  2:5246669/140‑1 (MQ=255)
     tataATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATgg                                                                                                                                       <  2:3679627/140‑1 (MQ=255)
       taataaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCa                                                                                                                                     <  1:5189390/140‑1 (MQ=255)
        aataaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGGTGCTGCCACACCCGGCCGCTTTGCCATGGCaa                                                                                                                                    <  1:2339545/140‑1 (MQ=255)
          taaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAg                                                                                                                                  <  2:2350408/140‑1 (MQ=255)
          taaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAg                                                                                                                                  >  1:1394029/1‑140 (MQ=255)
          taaAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAg                                                                                                                                  <  2:3030178/140‑1 (MQ=255)
            aaaaaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGAATTCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAAgt                                                                                                                                >  1:800994/1‑140 (MQ=255)
               aaaGTGTGCAAACCATGCATCTTTTAAATAAGTCTTGCAAGATACGTCTATTTTCTGTAGAATGGTGTATGTTGGTCTTTGACTGCGATGAAGGGAGAGGTTGCTGACACACCCGGCCGCTTGGCCAGGGCAAAGgtgtg                                                                                                                             >  2:709962/1‑139 (MQ=255)
               aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            >  2:1809230/1‑125 (MQ=255)
               aaaGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGtt                                                                                                                             <  1:1185462/140‑1 (MQ=255)
                    gtgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTGAGGt                                                                                                                        >  2:4660318/1‑140 (MQ=255)
                    gtgCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCATGGCAAAGGTGTTGAGGt                                                                                                                        >  1:6581153/1‑140 (MQ=255)
                         aaCCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  1:4349451/140‑1 (MQ=255)
                         aaCCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:3972757/140‑1 (MQ=255)
                         aaCCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:194174/140‑1 (MQ=255)
                         aaCCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTtc                                                                                                                   <  2:7393080/140‑1 (MQ=255)
                               gCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTGAGGTTTTTCTCACgg                                                                                                             >  1:289489/1‑140 (MQ=255)
                                 atatTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGgag                                                                                                           <  1:6891801/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTTCAACATACGTCTATTTTCTGTATATTGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:1329166/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:6774213/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:4728923/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:4245673/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:2750587/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  1:1780244/140‑1 (MQ=255)
                                   atTTTAAATAAGTCTAGCAACATCCGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGaa                                                                                                         <  2:2532331/140‑1 (MQ=255)
                                     tttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACt                                                                                                       >  2:6602429/1‑140 (MQ=255)
                                     tttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACGGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACt                                                                                                       >  2:1612609/1‑140 (MQ=255)
                                      ttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCAGGGCAAGGGGTTGAGGTTTTTATGACGGAGAAcgg                                                                                                      >  1:5034064/1‑138 (MQ=255)
                                      ttAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTg                                                                                                      >  2:7003896/1‑140 (MQ=255)
                                      ttAAATAAGTCTTGCAAAATACGTCTATTTTCTGTAAAATGGTGTATGTTGTTCTTAGACTGCGATGAAGTTAGAGGTTGCTGACACACACGGCCGCTTTTCGAATGCAAAGGGTTTCAGGTTTTTCACACGGAGaaaat                                                                                                      >  2:413249/1‑137 (MQ=255)
                                       tAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGt                                                                                                     <  1:5585127/140‑1 (MQ=255)
                                             aGTCTTGCAACATACGTCCATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACt                                                                                               <  2:6036309/140‑1 (MQ=255)
                                               tCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCCTGGGCAAGGTGTTCAGGTTTTTATCACGGGGAACGGTCGAACTTg                                                                                             >  1:72389/1‑140 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCCTGGGAAAGGTGGTCAGGTTTTTATGACGGCGAACTGTCGAACTTGCTGTAGGCgg                                                                                   >  1:2087239/1‑139 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAAATTTATGTAGGCGa                                                                                   >  2:6878911/1‑140 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTATGCGa                                                                                   >  2:719600/1‑140 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAGGGCCAAGGTGTTGAGGTTTTTGTGACGGAGAAAGGTATAAAGTGATGGAGTCgg                                                                                   >  1:6823112/1‑139 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCCTGGCAAATGTGGTGAGGTTTTTATGACGGGGAACTGTAGAAATTTCTGTAGGGGa                                                                                   >  1:6267948/1‑140 (MQ=255)
                                                         tACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCAGGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACATTCAAAATTTATGTTGGCgc                                                                                   >  1:1110415/1‑139 (MQ=255)
                                                            gTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTAAGGTTTTTATCACGGAGAACTGTCGAACGTGATGGAGGCGaaaa                                                                                >  1:5758839/1‑140 (MQ=255)
                                                             tCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAag                                                                               <  1:1517652/140‑1 (MQ=255)
                                                              cTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAagg                                                                              <  1:7545304/140‑1 (MQ=255)
                                                                 ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCAAGGCAAACGTGTTCAGGTTTTTCTAAAGGAGAACAGTATAAAATGAAGTAGGCGAAAaggagg                                                                           >  1:7351149/1‑140 (MQ=255)
                                                                 ttttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAATGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATTTAGGCGAAAaggagg                                                                           >  2:5340620/1‑140 (MQ=255)
                                                                  tttCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAggg                                                                          <  1:330704/140‑1 (MQ=255)
                                                                      tGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAAATGTCTAACTTTATGTAGGCGAAAAGGAGGGaaaa                                                                      >  2:2835547/1‑140 (MQ=255)
                                                                        tataATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTGCCTGGCCAAAGTGTTTAGGTTTTTGTCACGGAGAACATTGTAAATTGTTTTAGGCGGAAAGGGGGGaaaaaa                                                                    >  1:1005434/1‑138 (MQ=255)
                                                                             tGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCACAGGTGTTCAGGTTTTTCTCACGGAGAACTATCTAACTTGATGTAGGCGAAAATGAGGGAAAATAATTGc                                                               >  2:1154307/1‑140 (MQ=255)
                                                                                tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            <  2:4814737/60‑1 (MQ=255)
                                                                                tgtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATTGcaaa                                                            >  2:3132806/1‑140 (MQ=255)
                                                                                 gtATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCAAGGCAAAGGTGTTGAGGTTTTTCTAACGGAGAACTGTGTAAAATGATGTAGGCGACACGGAGGGGCAATAAAGGcaaaa                                                           >  1:3899925/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTGCGGTTTTTCTCACGGAGAACTGTCTAACTTGATGGAGGCGAAAAGGAGGGGAAATAAATGcaaaac                                                          >  1:6704042/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTGTCAAGGAGAAATTTATAAAGTGATTTATGCGAAAACGAGGGAAAAAAATGTcaaaaa                                                          >  1:3152851/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCAAAGGTGTTCAGGTTTTTCTAACGGACAACAGTATAAATTGATTTAGGCGAAAAGGAGGGAAAATAATGGcaaaac                                                          >  1:6482730/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCAGGGCAAAGGGGTTGATGTTTTTTTAACGGAGAAATGTCTAACGTGATGTAGGCGAAAAGGAGGGAAAATAATGTcaaaac                                                          >  1:7171761/1‑140 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTACAAGGGAAAATTGTTAAGGTTTTTATAACGGAGAACTGTATAACATGATGTAGGGGAAAACAAGGTGAAATAATAGcaaaaa                                                          >  1:4471032/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGGCCTGGGAAAGGTGTTCAGGTTTTTGTGACGGAGAACAGTATAACTTGATGTAGGCGAAAAGGAGGGAAAAAAATTGcaaaaa                                                          >  1:2997604/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATTGCCAAGGTGTTCAGGTTTTTATCAAGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGCAAATAATTGcaaaaa                                                          >  1:1982454/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCCAGGGCAAAGGTGTTAACGTTTTTGTCACGGAGAACTGTCTTACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGccaaaa                                                          >  1:5861214/1‑139 (MQ=255)
                                                                                  tATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTGCACGTGCAAACGTGTTAAGGTGTTTGTAACGGAGAACGGTCTAAATTGATTGAGGCGAAAATGAGGGCAAATAATAGgaaaaa                                                          >  1:2502557/1‑139 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAat                                                   >  2:3846071/1‑140 (MQ=255)
                                                                                         gTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTCCATGGCAAAGGTGTTCAGGTTTTTCTAACGGAGAACGTTCTAAATTGATGTTGGCGGAAAGGCGGGAAAAAAAATGGAAAACAAAAAat                                                   >  1:5458227/1‑140 (MQ=255)
                                                                                              tGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAATGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcgta                                              >  2:2091199/1‑140 (MQ=255)
                                                                                                aCTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAattcgtatt                                            <  2:2421043/140‑1 (MQ=255)
                                                                                                   gCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCGTTTGCATGGCAAAGGGGTTGAGGTTTTTGTGACGGTGAACAGTCTAAATTTATTTATGCGGAAAGGAGGGCAAAAAAATGCAAAACAAAAAattagtattggt                                         >  1:7099294/1‑140 (MQ=255)
                                                                                                    caaTGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGc                                                                                                                                            >  1:6151470/3‑40 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTTCCATGGCACAGGTGTTCAGGTTTTTCTGACGGAGAAATGTCTAACATGCTGTAGGCGAAAAGGCGGGGAAATAATTGCAAAAAAAAAAATTAGTTTTGGtt                                        >  1:4631896/1‑140 (MQ=255)
                                                                                                    cGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTCATGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAATGAGGGAAAATAATGTCAAAAAAAAAAATTCGGATTAGtt                                        >  1:3600696/1‑140 (MQ=255)
                                                                                                      aTGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCCAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAAAAATGGCAAAAAAAAAAATTCGTATTCGTTTg                                      >  2:2589892/1‑140 (MQ=255)
                                                                                                       tGAAGTGAGAGGTTTCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                     <  2:4944002/140‑1 (MQ=255)
                                                                                                       tGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGa                                     <  2:3315437/140‑1 (MQ=255)
                                                                                                        gAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGaa                                    <  1:6990185/140‑1 (MQ=255)
                                                                                                        gAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGaa                                    <  1:1607076/140‑1 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTTGCATGGCACATGTGTTCAGGTTTTTCTAACGGATAAATGTCTAAAATTATGTTTGCGAAAAGGCTGGAAAATAATGTCAAAAAAAAAAATTCGTATTAATTTGAAAGCa                                >  1:4886965/1‑140 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  1:5994779/140‑1 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  2:225852/140‑1 (MQ=255)
                                                                                                            tGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCa                                <  1:527972/140‑1 (MQ=255)
                                                                                                              agagGTTGCTGACACACCCGGCCGCTTTGCCCTGGGAAAGGTGTTCCGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATTATGGCAAAACAAAAAATTCCTATTCGTTTGAAAGCata                              >  2:5486032/1‑140 (MQ=255)
                                                                                                              agagGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCata                              <  2:4296650/140‑1 (MQ=255)
                                                                                                                  gTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGAt                          <  2:774707/140‑1 (MQ=255)
                                                                                                                   ttGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATc                         >  2:139779/1‑140 (MQ=255)
                                                                                                                     gCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAAAAAAAAATTCGTATTCGGTTGAAATCATATGATCAt                       >  2:6446363/1‑140 (MQ=255)
                                                                                                                      cTGACACACCCGGCCGCTTTGCCCTGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGTTGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                      >  2:3498457/1‑140 (MQ=255)
                                                                                                                      cTGACACACCCGGCCGCCTTGCCTTGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAACGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATa                      <  2:6159414/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:5095570/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:7567423/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  2:6792628/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:2648368/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:855926/140‑1 (MQ=255)
                                                                                                                       tGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAg                     <  1:6355681/140‑1 (MQ=255)
                                                                                                                         acacacCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTATCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTGGTTTGAAAGAATATGATCATAGaa                   >  2:5943304/1‑140 (MQ=255)
                                                                                                                           acacCCGGCCGCTTTGCCCAGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGTTGTAGGCGAAAAGGAGGGAAAATAAAGGCAAAAAAAAAAATTCGTATTCGGTTGAAACCAAATGATAATAGAAta                 >  2:459540/1‑139 (MQ=255)
                                                                                                                             acCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGGATTCGGTTGAAAGCATATGATCATAGAATCCt               >  2:5731742/1‑140 (MQ=255)
                                                                                                                                        tttGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGCGAACTGTCTAACTTGATGGAGGCGAAAAGGAGGGAAAAAAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATGCTTGATCAATCt     >  2:446898/1‑140 (MQ=255)
                                                                                                                                        tttGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGAGGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTACTTTTAAAGCATATTATAATAGAAACCTTGATCaaaat     >  2:2702570/1‑137 (MQ=255)
                                                                                                                                          tGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGGTTGAAAGCATATGATCATAGAATCCTTGATCACTCTGc   >  2:6349166/1‑140 (MQ=255)
                                                                                                                                           gCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCa  <  1:3477909/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                           
ACCTGTATAATAAAAAAAGTGTGCAAACCATGCATATTTTAAATAAGTCTTGCAACATACGTCTATTTTCTGTATAATGGTGTATGTTGGTCTTTGACTGCGATGAAGTGAGAGGTTGCTGACACACCCGGCCGCTTTGCCATGGCAAAGGTGTTCAGGTTTTTCTCACGGAGAACTGTCTAACTTGATGTAGGCGAAAAGGAGGGAAAATAATGGCAAAACAAAAAATTCGTATTCGTTTGAAAGCATATGATCATAGAATCCTTGATCAATCTGCA  >  NZ_CP034943/134979‑135256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: