Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,669,737 C→T 100% Q88* (CAG→TAG)  fliM → flagellar motor switch protein FliM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,669,7370CT98.9% 1260.8 / ‑0.2 348Q88* (CAG→TAG) fliMflagellar motor switch protein FliM
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base T (196/147);  total (200/148)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 348 total aligned reads displayed.

TCAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACCAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTAAT  >  NZ_CP034943/1669600‑1669873
                                                                                                                                         |                                                                                                                                        
tCAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAg                                                                                                                                        <  2:7120020/140‑1 (MQ=255)
 cAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAgg                                                                                                                                       >  2:4177552/1‑140 (MQ=255)
         cgcTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTccc                                                                                                                                   >  2:1693399/1‑136 (MQ=255)
         cgcTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTAt                                                                                                                               >  2:4800048/1‑140 (MQ=255)
               ggTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGaa                                                                                                                         <  1:6392582/140‑1 (MQ=255)
                gTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAAt                                                                                                                        <  2:475336/140‑1 (MQ=255)
                        aGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGa                                                                                                                >  1:5214333/1‑140 (MQ=255)
                         ggATCAGATCCTCAGTCTAACGATAATTCATGACAATTTTGCAAGACTTCTTAATACTCACTTTTCTGCTCATCTCAGGACCTTTTTTCACATTTCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                               <  2:5700624/140‑1 (MQ=255)
                         ggATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                               <  2:585619/140‑1 (MQ=255)
                         ggATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGat                                                                                                               <  2:5214333/140‑1 (MQ=255)
                           aTCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGatcc                                                                                                             >  2:5654489/1‑140 (MQ=255)
                           aTCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGatcc                                                                                                             >  2:375362/1‑140 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGTCTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                   >  1:1533176/1‑140 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                   >  1:744568/1‑140 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                   >  2:4056201/1‑140 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                   >  1:5886296/1‑140 (MQ=255)
                                     cAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTGATGAGGAATTTATCAGATCCATCCCGAACa                                                                                                   >  1:3660117/1‑140 (MQ=255)
                                        tCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACCAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGa                                                                                                <  2:6904800/140‑1 (MQ=255)
                                            aCGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCGTATGAGGAATTTATCAGATCCATCCCGAACATGACGAt                                                                                            >  1:4740275/1‑140 (MQ=255)
                                            aCGAGAATTCATGACAATGGTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGAt                                                                                            >  1:6219880/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                      >  1:3541736/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                      >  1:698045/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                      >  2:7370644/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATACCGAACATGACGATTTTGaa                                                                                      >  2:6420529/1‑140 (MQ=255)
                                                  aTTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCACTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGaa                                                                                      >  2:3018156/1‑140 (MQ=255)
                                                   ttCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAAt                                                                                     <  1:893394/140‑1 (MQ=255)
                                                               ttGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTc                                                                         <  2:1803796/140‑1 (MQ=255)
                                                                  cAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCAt                                                                      >  2:7347155/1‑140 (MQ=255)
                                                                   aaGACTTCTTACTACTCACTTTTCTGCTCATCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGACGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATc                                                                     <  1:6143873/140‑1 (MQ=255)
                                                                   aaGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATc                                                                     <  2:2130848/140‑1 (MQ=255)
                                                                   aaGACTTCTTACTACTCACTTGTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATc                                                                     <  2:2655530/140‑1 (MQ=255)
                                                                   aaGACTTCTTACTACGCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATc                                                                     <  2:4537707/140‑1 (MQ=255)
                                                                      ccttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGa                                                                  <  2:3299534/139‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  1:4158336/140‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  2:7006341/140‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  1:1638210/140‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  2:247482/140‑1 (MQ=255)
                                                                        ttcttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCGTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  2:4740275/140‑1 (MQ=255)
                                                                          cttACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTCTCAGCTCTGTTGACTAGGTCCCTTATGAGGAAGTTATCAGATCCATCGCGAACATGACGATGTTGAAGAGGGTTGAGGTGCATCAGATCGa                                                              >  2:5940263/1‑140 (MQ=255)
                                                                             actcctGACTTTTCGGCTCAGCTCAGGACCTATATTCACATTTCTGTCAGCTCTGTTGACTAGGTCCCTTCTGAGGAATTTATCAGATCCATCCCGAACATGTCGCATTTGAATCTGTTTGACGTCCATCCGATGGaatg                                                           <  1:5670537/140‑3 (MQ=255)
                                                                             actactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                           <  1:2894824/140‑1 (MQ=255)
                                                                             actactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                           <  2:3792688/140‑1 (MQ=255)
                                                                              ctactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTATGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGACTCTGTTTGACGTCCATCCGATGGAAGGt                                                          <  1:5419914/140‑1 (MQ=255)
                                                                              ctactCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGt                                                          <  1:318742/140‑1 (MQ=255)
                                                                                 ctCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTTAATCTGTTTGACGTCCATCCGATGGAAGGTAgg                                                       >  1:6716883/1‑140 (MQ=255)
                                                                                 ctCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGt                                                          <  1:4683620/137‑1 (MQ=255)
                                                                                 ctCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAgg                                                       >  2:5032469/1‑140 (MQ=255)
                                                                                    aCTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                >  1:2570561/1‑128 (MQ=255)
                                                                                    aCTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATg                                                                <  2:2570561/128‑1 (MQ=255)
                                                                                           tGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGAc                                                                                               <  1:6507047/90‑1 (MQ=255)
                                                                                                agctcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGtt                                        <  2:6589716/140‑1 (MQ=255)
                                                                                                agctcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGtt                                        <  2:6200317/140‑1 (MQ=255)
                                                                                                agctcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGtt                                        <  2:3330841/140‑1 (MQ=255)
                                                                                                agctcagGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGtt                                        >  1:5953498/1‑140 (MQ=255)
                                                                                                     agGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTCTGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCTTCCGATGGAGGGTCGGATTAGGATGGCGGTTAAccc                                   >  2:3912495/1‑140 (MQ=255)
                                                                                                     agGACCTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAAccc                                   >  2:5974080/1‑140 (MQ=255)
                                                                                                          cTATATTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACaa                              <  1:4402618/140‑1 (MQ=255)
                                                                                                           tataTTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAt                             <  2:1533176/140‑1 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGACGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  1:5516726/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTCGGATTATGATGGAGGTTATCCCGACAAtt                            >  1:5739936/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:959372/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:872628/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  1:2223602/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:1160763/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  1:243180/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  1:736119/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:7669565/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:294152/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTAGGATGGAGGTTAACCCGACAAtg                            >  2:6543816/1‑139 (MQ=255)
                                                                                                            atatTCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATAAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAAtt                            >  2:2864355/1‑140 (MQ=255)
                                                                                                            atatTCACATATCTGGCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAGTATGATGGAGGTTAACCCGACAAtt                            >  2:1483469/1‑140 (MQ=255)
                                                                                                               ttCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCa                         <  2:5458742/140‑1 (MQ=255)
                                                                                                               ttCACATATCTGTCAGCTCTGGTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCa                         <  2:1624354/140‑1 (MQ=255)
                                                                                                                tCACATATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCat                        <  1:7285673/140‑1 (MQ=255)
                                                                                                                 cacaTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCata                       <  1:6940401/140‑1 (MQ=255)
                                                                                                                   caTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCAtata                     <  1:6369530/140‑1 (MQ=255)
                                                                                                                   caTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCAtata                     <  1:4898752/140‑1 (MQ=255)
                                                                                                                   caTATCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCAtata                     <  1:7669565/140‑1 (MQ=255)
                                                                                                                   aatatCTGTCAGCTCTGTTGACTAGGTCCGTTATGAGGAATTTATCAGATCAATACCGAACATGACGATTTTGAATCTGTTTGAAGTCCATCCGATGGAAGGTAGGATTATTATGTAGGTTAACCCGACAATTGCAtata                     <  1:7187005/139‑1 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGTGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGGTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATAc                    >  2:4477136/1‑140 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACa                               <  2:2491719/129‑1 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACACGACAATTGCATATAc                    >  2:2032729/1‑140 (MQ=255)
                                                                                                                    atatCTGTCAGCTCTGATGACTAGGTCCCTTATGAGGAATTTATCAGATCAATCCCCAACATGAAGATTTTGAATCTGTTTGACGTCCATCCTATGTAAGGTAGGATTATGATGGAGGTTAACAAGACAATTTCATATAc                    >  2:6816625/1‑140 (MQ=255)
                                                                                                                       tCTGTCAGTTCTGTTGATCAGGTTCCGTATGAGGAATTTATCAGATCGATTCCAAACATGACGATTCTGAATCTATTTGATGTTCATCCGATGGAAGGAAGAATTATGATGGAGGTCAACCCCACGATAGCTTATAAgat                 >  2:423799/1‑140 (MQ=255)
                                                                                                                       tCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACgat                 >  1:2964182/1‑140 (MQ=255)
                                                                                                                       tCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACgat                 >  2:3532427/1‑140 (MQ=255)
                                                                                                                       tCTGTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACgat                 >  2:3123098/1‑140 (MQ=255)
                                                                                                                          gTCAGCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACgatgat              >  2:5414973/1‑140 (MQ=255)
                                                                                                                              gCTCTGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGAt          <  1:1251886/140‑1 (MQ=255)
                                                                                                                               ctctGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATc         <  2:2223602/140‑1 (MQ=255)
                                                                                                                                 ctGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCgg       <  1:3995522/140‑1 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                           >  2:1400196/1‑87 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                           <  1:1400196/87‑1 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                     >  1:7577446/1‑93 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                     <  2:7577446/93‑1 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGAt                                                     >  1:2058864/1‑93 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCggg      >  1:6065715/1‑140 (MQ=255)
                                                                                                                                  tGTTGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCggg      >  2:3647891/1‑140 (MQ=255)
                                                                                                                                    ttGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAgg                                                           <  1:4489355/85‑1 (MQ=255)
                                                                                                                                    ttGACTAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTa    >  2:5891167/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TCAAACGTGCGCTGCGGTTTTCCAAGGATCAGATCCGCAGTCTAACGAGAATTCATGACAATTTTGCAAGACTTCTTACTACTCACTTTTCTGCTCAGCTCAGGACCTATATTCACATATCTGTCAGCTCTGTTGACCAGGTCCCTTATGAGGAATTTATCAGATCCATCCCGAACATGACGATTTTGAATCTGTTTGACGTCCATCCGATGGAAGGTAGGATTATGATGGAGGTTAACCCGACAATTGCATATACGATGATGGATCGGGTAAT  >  NZ_CP034943/1669600‑1669873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: