Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,800,874 A→C 9.2% S3519R (AGC→CGC)  EO946_RS09135 → SDR family NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,800,8740AC 9.2% 610.2 / 9.5 243S3519R (AGC→CGC) EO946_RS09135SDR family NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (138/70);  new base C (2/33);  total (140/103)
Fisher's exact test for biased strand distribution p-value = 4.12e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 252 total aligned reads displayed.

ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGCCG  >  NZ_CP034943/1800738‑1801013
                                                                                                                                        |                                                                                                                                           
aTTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCg                                                                                                                                          >  2:7239303/1‑140 (MQ=255)
      cGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGc                                                                                                                                    >  2:1216256/1‑140 (MQ=255)
                 aaaCGGATCAAAAAGAGATCATCTAAGAATTTTACCGGGATTATGCGACTGAGCCACAAAAGAACCCTGAAGCTTTTATGGAAATTCATTCAGATAACGTCCCAGAAAACAATAATATAATCGACCGGCAATCTCACGcc                                                                                                                         <  2:1356920/139‑1 (MQ=255)
                  aaCGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAATCGACCGGCAATCTCACGCCg                                                                                                                        >  1:3550746/1‑140 (MQ=255)
                    cGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAg                                                                                                                      >  1:1030749/1‑140 (MQ=255)
                    cGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAg                                                                                                                      >  1:2544177/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  1:2189073/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  2:6596399/1‑140 (MQ=255)
                       aTCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCt                                                                                                                   >  1:5251689/1‑140 (MQ=255)
                         cacaAAGCCCTGATCGAACCATTTTACCGGGATGATGCGTCTGAGGCACAAAACCCCCCTTAACCTTATATGGAAATTCCTGCAGCTCTCGTACCACAAAACAAAAATATAAGCGACCGGCAATCTCACGCCGAGCCTAt                                                                                                                 <  2:7063705/140‑1 (MQ=255)
                         cacaAAGAGCTGATCGAAGCATTTTCCCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCACAAAACAATAATCTACGCGACCGGCAATCTCACGCCGAGCCTAt                                                                                                                 <  2:166366/140‑1 (MQ=255)
                         aacaAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTAt                                                                                                                 <  2:5458057/139‑1 (MQ=255)
                            aaccagCTGATCCAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCATAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                              <  2:6458750/136‑1 (MQ=255)
                            aaaGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGc                                                                                                              <  2:2189073/140‑1 (MQ=255)
                              agcgCTGATCGAAGCATTTTACGGGGTTCATGCTTCTGAGCCAAAAAACCCCCCGGAAGCTTATATGCAAATTCCTGCAGATCTCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCa                                                                                                            <  2:2937020/137‑1 (MQ=255)
                                 gCTGATCGTAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  2:601833/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCctta                                                                                                         >  1:2032606/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCattc                                                                                                         >  2:3801502/1‑139 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  2:2426027/1‑140 (MQ=255)
                                 gCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCatta                                                                                                         >  2:2520897/1‑140 (MQ=255)
                                  cTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCattat                                                                                                        >  2:1414217/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:2655578/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  2:4076349/1‑140 (MQ=255)
                                     aTCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGATCTCGTACCAGAAAACAATAATATAATCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTgg                                                                                                     >  1:2741742/1‑140 (MQ=255)
                                      tCGAATCATTTTACCGGGATTTTGCTTCTGAGGCACAAAAGCCCACTGAAGCTTATATGGAATTTCCTGCAGCTCTCGTACCATAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGc                                                                                                    <  2:2487151/140‑1 (MQ=255)
                                       tgAAGCATTTTACCGGGATGATGCATCAGAGGCGCAAAAGCCCCCTGAAGCTTATGCAGTAATTCCTGTAGCTTTAGAACCAGAAGCCAGTAAAAAAAGCATTCGGCAGGTTCACGATGAGCCTATTGCCATTATCGGCa                                                                                                   <  2:189589/139‑1 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:6929415/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  2:6101630/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:5542323/1‑140 (MQ=255)
                                          aGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGa                                                                                                >  1:5985687/1‑140 (MQ=255)
                                            cATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATACTATAAGCGACCGGCAATCACACTCCGAGCCTATCGCACTTATTGTCATGAgg                                                                                              >  2:4173666/1‑139 (MQ=255)
                                            cATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAgg                                                                                              >  2:6801092/1‑139 (MQ=255)
                                              ttttAGGGGTATGATGCGTCTGAGCCACAAAACCCCCCTGAAGCTGATTTGGAAATTCCTGCTGCACTCGTACCATAAAACAATAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTgg                                                                                            <  1:4775704/140‑1 (MQ=255)
                                                  cccGGGATGATGCGTCTGAGCCACAAAACCCCCCTCAAGCTGATTTGGAAATGCGTGCCCCTCTCGTACCACAAACCACTAATACACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAgg                                                                                        <  1:6724253/139‑1 (MQ=255)
                                                  aCCGGGCTGCGGCGTCTGAGCCACAAACCCCCCCTGACCCTGATATGGACAGTCGTGCAGCACTCGTACCAGAAAACAACACTCTCCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAgg                                                                                        <  1:6570057/140‑1 (MQ=255)
                                                     gggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGttt                                                                                     >  1:579001/1‑140 (MQ=255)
                                                      ggATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTc                                                                                    >  1:1896690/1‑140 (MQ=255)
                                                        atgtggCGTCTGCGCCACAAACGCCCCCGGACCCTCATCTGTAACTTCCTGCAGCTCCCGCACCACAAAACAATAATAACCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:6101630/135‑1 (MQ=255)
                                                        aggatgCGTCTGAGTCACAAAAGCCCCCTCAAGCTCATTTTCAAATTCGTGAAGCTCTCGTACCCGAAAAAAATAAGATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCt                                                                                  <  1:7618092/138‑1 (MQ=255)
                                                            tgCGTCTGAGGCACACAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATAGAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTGTCCTCAAg                                                                              <  2:1463574/140‑1 (MQ=255)
                                                            ggCGTCTGAGGCACAAACGCCCCGTGACGGGGATAGGGACAGGCCTGCCGCTCTCGTCCCCGAAAACACTCATCTCAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCCAg                                                                              <  2:3690181/139‑1 (MQ=255)
                                                               gTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTg                                                                           >  1:7266733/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  2:7013969/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  2:826626/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  1:2298331/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  1:4664805/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGa                                                                          >  2:4712892/1‑140 (MQ=255)
                                                                tCTGAGGCACAAAAGCCCCCTGAAGCTGAGATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTCTCGCCATTATTGGCAGGCGTGGCAGGTTTCCTCAAGCTGa                                                                          >  1:3008765/1‑140 (MQ=255)
                                                                  tGAGGCACAAAAGCCCCCTGAAGCTGATTTGGAAATTCCTGCCGCTCTCTTACCATAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGAtt                                                                        <  2:1970515/140‑1 (MQ=255)
                                                                   gAGGCACAAAAGCCCCCTTAAGCTTATATGTAAATTCCTGCAGCTCTCGTACAAGAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  2:6266479/140‑1 (MQ=255)
                                                                   gAGGCACAAAAGCCCCCTGAAGGTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  2:5231880/140‑1 (MQ=255)
                                                                   gAGGCAAAAAAGCCCCCTGAAGCTTATATGGAAATTCCGGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTc                                                                       <  2:133153/140‑1 (MQ=255)
                                                                      gCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGt                                                                    >  1:3594937/1‑140 (MQ=255)
                                                                        acaAAAGCCCCCTGAAGCTTATATGGAAATTCCTGCAGCTCTCGTACCACAAAACAATAATATCCGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTcc                                                                  <  1:708758/140‑1 (MQ=255)
                                                                          aaaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAt                                                                >  1:1647705/1‑140 (MQ=255)
                                                                          aaaaCCCCCCTTAAGGTTATATGTAAATTCCTGCAGCTCTCTTACCACAAAAAAAAAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAt                                                                <  1:2583787/140‑1 (MQ=255)
                                                                           aacgCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTATCGTACCAGAAAAAAATAATCTAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  1:5600625/137‑1 (MQ=255)
                                                                           aaaGCCCCCTGATCCTTTTATGTAAATTCCTGCAGCTCTCGTACCACAAAAAAATATTATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  1:1416558/140‑1 (MQ=255)
                                                                           aaaGCCCCCTGAAGCTGATATGTAAATTCCTGCAGCTCTCGTACCATAAAAAAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  1:1758797/140‑1 (MQ=255)
                                                                           aaaGCCCCCTGAAGCTGATATGGAAATTCCTGCTGCTCTCGTACCTGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  2:1730454/140‑1 (MQ=255)
                                                                           aaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCATAAAACAATACTCTAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  2:5587468/140‑1 (MQ=255)
                                                                           aaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  2:5542323/140‑1 (MQ=255)
                                                                           aaaGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCACAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               <  1:3923433/140‑1 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCCGAAAACAATAATAGACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTCTTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                         >  2:2525267/1‑140 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATg                                                               >  2:7267853/1‑134 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                         >  1:2417989/1‑140 (MQ=255)
                                                                                 cccTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTAt                                                         >  1:1223840/1‑140 (MQ=255)
                                                                                     gAAGCTGATATGGAATTTCCTGCCGCTCTCGTACCAGAAACCAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGa                                                     <  2:6915065/140‑1 (MQ=255)
                                                                                      aaGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGAc                                                    >  2:4888595/1‑140 (MQ=255)
                                                                                         cTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAAt                                                 >  1:1488923/1‑140 (MQ=255)
                                                                                         cTGATATGCAAATTCCTGCAGCTACCGTACCACAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAAt                                                 <  2:685533/140‑1 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTCTGGGACAATCt                                               >  1:763193/1‑140 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCt                                               >  1:4558301/1‑140 (MQ=255)
                                                                                           gATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCt                                               >  1:3260931/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:5324515/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:2334690/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:6323118/1‑140 (MQ=255)
                                                                                            atatGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTg                                              >  1:5451082/1‑140 (MQ=255)
                                                                                                  aattcccTGCACCTCTCTCACAATAAAAAAAAAATAAACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAAc                                        <  1:40339/135‑1 (MQ=255)
                                                                                                   aaTTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGCCAATCTGAAAAACg                                       >  1:7191774/1‑140 (MQ=255)
                                                                                                     cgccggCAGCTCGGGTCCCAGAAACCATTACTCTAAGCGCCCCGCACTCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTGTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGc                                     <  2:6377791/135‑1 (MQ=255)
                                                                                                      tCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCa                                    >  2:497758/1‑140 (MQ=255)
                                                                                                       ccTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCaa                                   >  1:4994949/1‑140 (MQ=255)
                                                                                                       ccTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCaa                                   >  1:6650654/1‑140 (MQ=255)
                                                                                                        cTGCAGCTCTCGTACCATAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCaaa                                  <  1:7289524/140‑1 (MQ=255)
                                                                                                          gCATCTCTCGTACCATCAAACACTCACAGACGCGACCGGCCAGCTCACGCCGAGCCTATCGCCAATATTGGCATGAGTGGCAGGTTGCCTCAAGCTGATTCGCTCCATGAGCTATGGGACAATCTGAAAAACCGCAAACa                                <  2:3315224/140‑1 (MQ=255)
                                                                                                          gCAGCTCTGGTACCAGAAAACAATACTATCAGCCACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGGTATGGGACAATCTGAAAAACGGCAAACa                                <  2:5317194/140‑1 (MQ=255)
                                                                                                          gCAGCTCTCGTACCACAAACCAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACa                                <  2:2439528/140‑1 (MQ=255)
                                                                                                                ctcGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGGAt                          >  1:6385182/1‑140 (MQ=255)
                                                                                                                ctcGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGATATGGGACAATCTGAAAAACGGCAAACATTGTAt                          >  1:4505784/1‑140 (MQ=255)
                                                                                                                    tACCAGAAAACAATAATATACGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATctct                      <  2:3418547/140‑1 (MQ=255)
                                                                                                                    tACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATctct                      <  2:3142741/140‑1 (MQ=255)
                                                                                                                          aaaaCAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATc                >  2:6773415/1‑140 (MQ=255)
                                                                                                                                      taAGCGACCGGAAATCTCACGCCGAGCCTATCTCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTTCATGAGCTATGTGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCAGAAAGATcgc    <  2:5288147/140‑1 (MQ=255)
                                                                                                                                      aaaGCGACCGGCAATGTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGCTTCGGTCCATGAGCTATGGGACAATCTGAAAACCGGGAAACATTGTATCTCTGACATCCCTACAGAAcgc    <  2:4385831/139‑1 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  2:5710144/1‑140 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  1:3168085/1‑140 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  1:6633224/1‑140 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  1:6902382/1‑140 (MQ=255)
                                                                                                                                        aGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCAGGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAAcgccg  >  2:3483387/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
ATTGATCGGCTTCTTCTTAACGGATCACAAAGAGCTGATCGAAGCATTTTACCGGGATGATGCGTCTGAGGCACAAAAGCCCCCTGAAGCTGATATGGAAATTCCTGCAGCTCTCGTACCAGAAAACAATAATATAAGCGACCGGCAATCTCACGCCGAGCCTATCGCCATTATTGGCATGAGTGGCAGGTTTCCTCAAGCTGATTCGGTCCATGAGCTATGGGACAATCTGAAAAACGGCAAACATTGTATCTCTGACATCCCGAAAGAACGCCG  >  NZ_CP034943/1800738‑1801013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: