Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 1,936,589 T→G 10.8% K3554N (AAA→AAC EO946_RS09735 ← non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349431,936,5890TG10.8% 972.6 / 14.7 413K3554N (AAA→AACEO946_RS09735non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base T (133/212);  new base G (65/2);  total (198/215)
Fisher's exact test for biased strand distribution p-value = 2.78e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

CGAGTCCAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGC  >  NZ_CP034943/1936452‑1936722
                                                                                                                                         |                                                                                                                                      
cGAGTCCAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGtgtt                                                                                                                                      >  1:2829006/1‑140 (MQ=255)
        aGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTg                                                                                                                              >  1:5626540/1‑140 (MQ=255)
           aTCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCct                                                                                                                           <  1:2433053/140‑1 (MQ=255)
                 aGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTctcctc                                                                                                                     >  2:3110068/1‑140 (MQ=255)
                   aaaCTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAATTTCCTCTCCTCCa                                                                                                                   >  1:2124746/1‑140 (MQ=255)
                        tCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGTAAGTGGCTCTCCTCCAATTaa                                                                                                              >  2:6061891/1‑140 (MQ=255)
                                 aaCAGGGACGGACCGCCACACGTGTCGGTTGGTCCCTATACAATTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTCTAGGAACTTGGTTACCGTCGATTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  2:7632353/140‑1 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTTCCGCTCCTCCAATTAAAACATGACg                                                                                                    >  1:497933/1‑140 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  2:425487/140‑1 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  1:4877959/140‑1 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  2:1549615/140‑1 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  2:4598391/140‑1 (MQ=255)
                                 aaCAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTGGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACg                                                                                                    <  1:4528926/140‑1 (MQ=255)
                                      ggACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCTCGCCTCCAAATAAAAGATGTCGAAGCg                                                                                               >  2:1518544/1‑140 (MQ=255)
                                        aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                             <  2:4670906/140‑1 (MQ=255)
                                        aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                             <  2:1893501/140‑1 (MQ=255)
                                        aCGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGt                                                                                             <  2:907767/140‑1 (MQ=255)
                                           cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCCAATAAGTGTCTGAACTTGGTTACCGTCGTTTTTTGCAGTGCCGCTCCGCCCAATAACACATGACGAAGCGGTACt                                                                                          >  2:2838181/1‑140 (MQ=255)
                                           cATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTCACCGCCAAATAAAACATGACGAAGAGGTAcg                                                                                          >  2:4330673/1‑139 (MQ=255)
                                           cATCGACACAAGTTTCGGGTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTTCCTCTCCTCCAATTAAAAGATGACGAAGCGGTAca                                                                                          >  2:7230214/1‑139 (MQ=255)
                                                   caAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcccc                                                                                  <  2:349125/140‑1 (MQ=255)
                                                   caAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTcccc                                                                                  <  2:7402687/140‑1 (MQ=255)
                                                      gTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCCAGTAACACATGAAGAATCGGTACTCCATCCCcaac                                                                               >  2:6685969/1‑138 (MQ=255)
                                                           ggTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTg                                                                          <  2:2386002/140‑1 (MQ=255)
                                                                gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTTCCGGGCCGCCCATTTACATATTACGAAGCGGTACTCCTTACCCCACATATGCAGcg                                                                     >  1:3292458/1‑139 (MQ=255)
                                                                gTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTTCCTCTCCTCCAATTAAAATAGGAAGAAGCGGTAATCCTTCCACATAATCTGCAGCt                                                                     >  1:749193/1‑140 (MQ=255)
                                                                   cATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGTAGTGGCGCTCCGCCCAATAAAACATGACGAAGCGGTACACCGTACCCAACATCTGCCGCGAcc                                                                  >  1:1145494/1‑140 (MQ=255)
                                                                     tctaCATTTGGGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCACTTAACAGATGACGAAGCGGTACTCATTCCCCATCATCTGCAGCTACcag                                                                <  1:7662546/138‑1 (MQ=255)
                                                                     tataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTTCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTACCCATCATCTGCAACTACcag                                                                >  2:6310674/1‑140 (MQ=255)
                                                                     tataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcag                                                                <  1:2976862/140‑1 (MQ=255)
                                                                     tataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcag                                                                <  1:3599113/140‑1 (MQ=255)
                                                                     tataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcag                                                                <  1:2288724/140‑1 (MQ=255)
                                                                      ataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcagc                                                               <  1:5146862/140‑1 (MQ=255)
                                                                      ataCATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACcagc                                                               <  1:4397967/140‑1 (MQ=255)
                                                                          aTTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCTCCGCCACTTAAAATATGACGCAGCGGTGCTCCGTCCCCCTCAAATGCAGCGACcagcaccg                                                           >  1:3086173/1‑137 (MQ=255)
                                                                          aTTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCACGCCGCCCAATAACAGATGACGGAGCGGTACTCCATCCCCATAATCTTCACCGACCagccgcg                                                           >  1:5521278/1‑139 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGGCTCTCCTGCAATTAACAGATTACGAAACGGTACTCCATACCCATCATCTGCAGCGAACaccagcgt                                                          >  1:3178519/1‑138 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGTAGTGGCGGTCCTGCCATTTAAAGATGAAGAAGCGGTACTCCTTCCCCCTCATCTGCAGCTACCagcagcgt                                                          >  1:5361937/1‑138 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCGGTACTCCCATTAACAAAATTACAAGCGGTAATCCTTACCCAACAGATGGACCGACcagcaacgt                                                          >  1:6888222/1‑136 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCtt                                                          >  2:4738018/1‑140 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAAAAGATGACGAAGCGGTTATCCTTCCCCAACATCTGCAGCTACCAGCAGCtt                                                          >  1:3734376/1‑140 (MQ=255)
                                                                           tttGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAAATAAAACATGACGAAGCGGTACTCCTTACCCCACATCTGCAGCTACCagcagcgt                                                          >  1:664232/1‑138 (MQ=255)
                                                                             tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTTCCGGTCCTCCCATTAAAAGATTACGAAGCGGTAATACTTCCCCATAATATGCAGCTACCAGCAGCTTCa                                                        >  1:960171/1‑140 (MQ=255)
                                                                             tgtgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCGCTCCGCCCAATAACAAATTACGAACCGGTACTCCATACCCAACATAAGCAGCGACCAGCAGCTTCa                                                        >  2:2931996/1‑140 (MQ=255)
                                                                               tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTAGCCTGCAAATAACAGATGACCAAGCCGTTATCCTTACCCATAATCTTCAGAAACCACCAGCATCaaa                                                      >  2:5320619/1‑140 (MQ=255)
                                                                               tgATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCGGTCCGCCAATTAACAGATGACGAAGCGGTACTGCTTCCCCCTCATCTGCAGCTACCCGCAGCTTCaaa                                                      >  2:5139271/1‑140 (MQ=255)
                                                                                   ggCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATggg                                                  <  1:7373432/140‑1 (MQ=255)
                                                                                        gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCACTCCTACAAATAAAAGATGAAGAAGCGCTACAACTTAACCAAAAAATTCAGCTAACAGCAGCTTAAAAAgggcgggg                                             >  2:1096590/1‑139 (MQ=255)
                                                                                        gTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATgggcgggc                                             >  2:1378638/1‑140 (MQ=255)
                                                                                           ctgctCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCAACATCTGCAGCGACCACCAGCTTCAAATGGGGGGGCGTc                                          >  2:7096768/1‑140 (MQ=255)
                                                                                            tgctCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTcc                                         <  1:6800647/140‑1 (MQ=255)
                                                                                              ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGGTGAGGAAGCGGTACTCCTTCCCCAACATATGCAGCTACCCGCACCTTCAAATGGGCGGGGGTGCCg                                       >  2:1168024/1‑140 (MQ=255)
                                                                                              ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCg                                       <  1:2618452/140‑1 (MQ=255)
                                                                                              ctctATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGGAGTGCCGCTCCGCCAATTAACAGATTGCGAAGCGGTACTCCTTCCCCCTCATCTGCAGCTACCCGCACCGTCAAATTGGCGGGCGTgccc                                       >  1:849771/1‑139 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGGAGTGCCTCTCGTACAATTAAAACATGAAGAAGCCGTACTCCTTCCCCATAATCTGCAGCTACAAGCACCTTCAAATGGGCGGGCGTGCCGTCggg                                  >  2:5538992/1‑139 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACAGATTACGAAGCGGTAAACCTTCCCCAAAATCTGCAGCTACCCGCAGCGTAAAATGGGCGGGCGTCCcggcggg                                  >  2:3334854/1‑139 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                  <  1:1688591/140‑1 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                  <  1:7581817/140‑1 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCGACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGt                                  <  2:6619261/140‑1 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCGCGCCTCCCATTAACAGATTACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCCGCGTCAACTGGGCGGGCGGCCCCGCGGt                                  >  2:7314330/1‑140 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGGAGTGCCTCTCCTCCCATTAAAACATTAAGAACCGGTAATACTTCCCCCGCAACTGCCCCTACCAGCCGCATCAAATGGGCGGGCGTGCcggcggg                                  >  1:1576427/1‑139 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGTAGTTCCGCTCCGGCAAATAAAAGATGACGAACCGGGACAACGTACCCCAAAACTGCAGCTAACAGCAGCGTCAAAAGGGCGGGCGGCCcggcggg                                  >  1:6348387/1‑139 (MQ=255)
                                                                                                   tCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTGGAAGTGGCTCTCCGCCCAATAACAGATGACGAAGCGGTACTCCTTGCCCATCATCTGCAGCTACCAGCACCGTCAAATGGGCGGGCGTCCCGGCGGt                                  >  1:7251792/1‑140 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAAGTGCCTCTCCTCCAATTAACACATTACGAAGCGGTACTACTTACCCATAATCTGCACCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGGTAAGt                             >  2:4092843/1‑140 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTTGAACTGCCGCTCCTCCAATTAACACATTACGAAGCGGTACGCCTTACCCATCATCTGCAGCGACCAGCAGCTTCAAATTGGCGGGCGTCCCGTCGGTTAcct                             >  2:5802810/1‑138 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGTAGTGCCGCTCCTCCAATTAAAAGATGACGAAGCGGTACTCCGTGCCCCTCATCTGCAGCTACCAGCAGCGTCAAATTGGCGGGGGTCCCGTCGGTTACgg                             >  1:3461797/1‑139 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATTACGAAACGGTAATCCTTGCCCCTCAACTGCAGCTACCAGCAGCGTCAAATGGGCGGGCGTCCCGTCGGGTAAGt                             >  2:3700048/1‑140 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGt                             <  1:4304648/140‑1 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTACCCAACATCAGCAGCTACCAGCAGCTTCAAAAGGGCGGGCGTCCCCTCGGTTAcct                             >  2:65585/1‑138 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAAATGAAGAAGCGGTACTCCGTCCCCATCAACTTCAGCGACCCGCAGCGTCAAATTGGCGGGCGTCCCGTCGGGTACgg                             >  2:3235323/1‑139 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCTCTGCGCCAATTAACAGATTACGAAGCGGTACTCCTTACCCATCATCTGCAGCTACCACCAGCTTAAAATGGGCGGGCGGCCCGTCCGTTACGt                             >  2:1231186/1‑140 (MQ=255)
                                                                                                        gcCAAATAAGTGTATGAACTTGGTTACCGTCGTGTTTTGAAGTGCCGCTCCGCCAAATAACACAAGACGAAGCGGTACTCCATCCCCATCAGCTGCACCTACCAGCACCTTCAAAAGGGCGGGCGGCCCGGGGGGTACGt                             >  1:464082/1‑140 (MQ=255)
                                                                                                           aaaTAAGTGTATGAACTTGGTTACCGTCGTGTTTTGTAGTGCCTCTCCTCCAAATAACAGATGACGAAGCGGTACTCCGTCCCCCACATCTGCAGCTAACCGCAGCTTCAAATGGGCGGGCGGCCCGTCGGTTAAGTCaa                          >  2:2882024/1‑140 (MQ=255)
                                                                                                             aTAAGTTTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGTGCGGGCGTCCCGTCGGTTACGTCAATg                        <  1:4033409/140‑1 (MQ=255)
                                                                                                               aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTACCAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                      <  1:7118056/140‑1 (MQ=255)
                                                                                                               aaGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCg                      <  2:5434771/140‑1 (MQ=255)
                                                                                                                  tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                   <  1:6589816/140‑1 (MQ=255)
                                                                                                                  tgtATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATg                   <  2:2538745/140‑1 (MQ=255)
                                                                                                                    tATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCt                 <  1:1040025/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  2:5278623/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  1:4771465/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  1:1378638/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  1:5057730/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  1:1952696/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  2:1663145/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  2:2728126/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  2:5866841/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  1:3527046/140‑1 (MQ=255)
                                                                                                                         aCTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGa            <  2:3551909/140‑1 (MQ=255)
                                                                                                                            tGGTTACCGTCGTTTTTGGAAATGCCTCTCCTCCAATTAACAGATGACGAATCGGTACTACTTCCCCCTCATCTGCAGCTACCACCAGCGTCAAATTGGGGGGCGTCCCCTCGGTTACCTCAATTCGATTCGGTCGATAg         >  2:2233200/1‑140 (MQ=255)
                                                                                                                              gTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTa       <  2:4934068/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  2:303016/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  1:4096543/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  1:6061891/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  1:5859434/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  2:7563083/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGGACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  2:1989292/140‑1 (MQ=255)
                                                                                                                               ttACCGTCGTGTTTGGACGTGCCTCTCCTCCAATGAACAGATGACGAAGCGGTACTCCTTCCCCAGCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTAt      <  2:3964133/140‑1 (MQ=255)
                                                                                                                                   cgtcgtTTTTTGAAGTGCCTCTCCTCCAATTAAAAAAAGACGAACCGGTACTCCTTCCCCATCAACTGCAGCAACCAGCAGCTTCAAATGGGCGGGCGTCCCCGCGGGTGAGGCAATGCGTTGCGGTCGATattatttgg  >  2:5684494/1‑137 (MQ=255)
                                                                                                                                   cgtcgtTTTTGGAAGTGCCTCTCCTCCAATTAACAGATTACGAAGCGGTACTCCTTCCCCATCATCTGCACCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAAAGCGATGCAGTCGATAGGAtttgg  >  2:7034891/1‑137 (MQ=255)
                                                                                                                                         |                                                                                                                                      
CGAGTCCAAGCATCCGCAGAAACTTCGATGTTGAACAGGGACGCATCGACACAAGTTTCGGTTGGTCCATATACATTTGTGATGGCAGGTGCTGCTCTATCCGCGCCAAATAAGTGTATGAACTTGGTTACCGTCGTTTTTGGAAGTGCCTCTCCTCCAATTAACAGATGACGAAGCGGTACTCCTTCCCCATCATCTGCAGCTACCAGCAGCTTCAAATGGGCGGGCGTCCCGTCGGTTACGTCAATGCGATGCTGTCGATAGTATTGGC  >  NZ_CP034943/1936452‑1936722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: