Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,169,199 C→G 100% G81G (GGG→GGC EO946_RS10950 ← class I SAM‑dependent methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,169,1990CG97.8% 1090.3 / 9.8 317G81G (GGG→GGCEO946_RS10950class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base G (162/144);  total (164/153)
Fisher's exact test for biased strand distribution p-value = 2.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 340 total aligned reads displayed.

TTTTCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCCCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCTGACTTCAGCAGGTTT  >  NZ_CP034943/2169062‑2169334
                                                                                                                                         |                                                                                                                                       
ttttCACTCCCAATCACAGAACACGTTTCAACTAGTGCCATACTTGCGATAATGGCATCCGAACCGAGACCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGGTcgcc                                                                                                                                       <  1:6219193/140‑1 (MQ=255)
 tttCAATCCCAATCACTGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTGCCCTTCCGATAAACCAGCGGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:3600315/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:2702326/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:7020965/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:6811476/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  1:194832/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:4561167/140‑1 (MQ=255)
 tttCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgccc                                                                                                                                      <  2:5513119/140‑1 (MQ=255)
       tCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATACTCGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATg                                                                                                                                <  2:4248287/140‑1 (MQ=255)
       tCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATg                                                                                                                                <  1:5261820/140‑1 (MQ=255)
       tCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATg                                                                                                                                <  1:4913127/140‑1 (MQ=255)
       tCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATg                                                                                                                                <  1:6509050/140‑1 (MQ=255)
         ccAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:4084549/140‑1 (MQ=255)
         ccAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:115542/140‑1 (MQ=255)
         ccAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:7465796/140‑1 (MQ=255)
         ccAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:804048/140‑1 (MQ=255)
            aTCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCg                                                                                                                           >  2:1568730/1‑140 (MQ=255)
                  gAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGTTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGc                                                                                                                     >  1:5085194/1‑140 (MQ=255)
                   aaCCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGct                                                                                                                    >  1:2960614/1‑140 (MQ=255)
                   aaCCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGct                                                                                                                    <  1:662370/140‑1 (MQ=255)
                   aaCCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGct                                                                                                                    <  2:173836/140‑1 (MQ=255)
                   aaCCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGct                                                                                                                    <  1:407715/140‑1 (MQ=255)
                     cccGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         >  1:3173761/1‑133 (MQ=255)
                     cccGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         <  2:3173761/133‑1 (MQ=255)
                        gTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         >  2:2134545/1‑130 (MQ=255)
                        gTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGaa                                                                                                               <  1:25839/140‑1 (MQ=255)
                         tttCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAAc                                                                                                              >  2:517492/1‑140 (MQ=255)
                          ttCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCGTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACa                                                                                                             <  2:5913606/140‑1 (MQ=255)
                           tCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACAt                                                                                                            <  2:2234149/140‑1 (MQ=255)
                               cTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCt                                                                                                        <  1:3586937/140‑1 (MQ=255)
                                 aCTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGt                                                                                                      >  1:2231570/1‑140 (MQ=255)
                                    gCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTAtt                                                                                                   >  2:3432349/1‑140 (MQ=255)
                                       aTACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCgg                                                                                                >  1:1032625/1‑140 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:1625988/104‑1 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              <  2:1332017/104‑1 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGaa                                                                                                                              >  1:1332017/1‑104 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         <  2:1529455/109‑1 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGaaa                                                                                          >  1:871973/1‑140 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGaaa                                                                                          >  2:3286384/1‑140 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGaaa                                                                                          >  2:3656375/1‑140 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGaaa                                                                                          >  1:2092580/1‑140 (MQ=255)
                                             gCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGaaa                                                                                          >  1:1732368/1‑140 (MQ=255)
                                                aTAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACAt                                                                                                            >  2:1795770/1‑119 (MQ=255)
                                                aTAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACAt                                                                                                            <  1:1795770/119‑1 (MQ=255)
                                                       cATCTGACCCCAACCCGAGGGTGCAATCTAAAAACGTATCCCCTTCAGATAAGCCGGCAGCCCGAAGCATAGGTTCTTTCTCCCCCTGTAAGAAGCGTTTGGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTg                                                                                <  1:3219768/140‑1 (MQ=255)
                                                         tCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgcc                                                                                                                                       >  2:966961/1‑83 (MQ=255)
                                                         tCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         >  2:7145380/1‑97 (MQ=255)
                                                         tCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         <  1:7145380/97‑1 (MQ=255)
                                                            gAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCAcc                                                                           <  1:517492/140‑1 (MQ=255)
                                                                 gAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGtt                                                                      <  2:1463566/140‑1 (MQ=255)
                                                                 gAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGtt                                                                      <  2:2468315/140‑1 (MQ=255)
                                                                  aGCCCGAGTGTACAATCTAAAAACGTGTCCCCTGCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGtt                                                                                                                         <  1:3901609/88‑1 (MQ=255)
                                                                   acccGAGTGTGCAATCTAAAAACGTATCCCCTTCAGATAAGCCGGCAGCCCGAAGCATAGGTTCTTTCTCCCCCTGTAAGAAGCGTTTGGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTc                                                                    >  2:1100692/2‑140 (MQ=255)
                                                                        aGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGtata                                                               >  2:5278736/1‑140 (MQ=255)
                                                                        aGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGtata                                                               <  1:5938337/140‑1 (MQ=255)
                                                                        aGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGGATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGGACCTTGTTTCGtata                                                               >  1:7058876/1‑140 (MQ=255)
                                                                        aGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCGGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGtata                                                               <  1:1526190/140‑1 (MQ=255)
                                                                            tACAATGTACAAACCTTTCCCCTAACGATAAACCTGCTGCCCGATGCATGGGCTCCTGCTCGCCCCCTAAGAACCCTTTACCTCTGAACATCGCTGTATTCGGATTAAAAAAGAATTACGCACCTTGGTTCGTATaacct                                                           <  2:3883828/140‑4 (MQ=255)
                                                                            tACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGt                                                           >  2:6365987/1‑140 (MQ=255)
                                                                                 tCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCTTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCaaa                                                      <  1:7554519/140‑1 (MQ=255)
                                                                                 tCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCaaa                                                      >  1:7209602/1‑140 (MQ=255)
                                                                                 tCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCaaa                                                      >  1:3400946/1‑140 (MQ=255)
                                                                                 tCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCaaa                                                      <  1:6044010/140‑1 (MQ=255)
                                                                                 tCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCaaa                                                      <  1:4566129/140‑1 (MQ=255)
                                                                                    aaaaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCg                                                   >  1:965813/1‑140 (MQ=255)
                                                                                    aaaaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCg                                                   >  1:7275643/1‑140 (MQ=255)
                                                                                    aaaaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGAATGAAGCGTTTAGCTCTGAACATCGCTGTATTCTGATGAAAAAAGAATTTGGCACATTGTTTCGTATAAAGTTAAAAcag                                                   >  1:5541096/1‑138 (MQ=255)
                                                                                      aaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTcgcc                                                                                                                                       >  1:5367270/1‑54 (MQ=255)
                                                                                      aaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGct                                                 <  2:6607268/140‑1 (MQ=255)
                                                                                      aaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGct                                                 >  1:3862132/1‑140 (MQ=255)
                                                                                      aaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGct                                                 >  2:5984002/1‑140 (MQ=255)
                                                                                      aaaCGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGct                                                 <  2:1260071/140‑1 (MQ=255)
                                                                                      aaaCGTGTCCCCGTCCCATAACCCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTAGTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGct                                                 <  2:3964147/140‑1 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTg                                            >  1:4297560/1‑140 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTg                                            >  1:4666486/1‑140 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTg                                            >  1:5776183/1‑140 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTg                                            >  2:4793962/1‑140 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGGTTCGTATAAAGTTCAAACCGCTCTTTg                                            >  1:2813460/1‑140 (MQ=255)
                                                                                           tgtCCCCTTCCGATAAACCACCTGCCCGAAGCATAGGCTCCTGCTCGCCCCTTATGAAGAGTTTAGCTCTGAACATCGCTGTCTTAGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCtttt                                            >  1:3996780/1‑139 (MQ=255)
                                                                                             tCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATACAGTTCAAACCGCTCTTTGcc                                          >  1:1408962/1‑140 (MQ=255)
                                                                                                  ttCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCa                                     <  1:3092377/140‑1 (MQ=255)
                                                                                                  ttCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCa                                     <  1:3365760/140‑1 (MQ=255)
                                                                                                  ttCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCa                                     <  1:1829081/140‑1 (MQ=255)
                                                                                                     cGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTa                                  <  1:2249617/140‑1 (MQ=255)
                                                                                                           aCCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAAt                            >  2:6690649/1‑140 (MQ=255)
                                                                                                           aCCAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGGATGAAGCGTTTAGCTCTGAACATCGCTGTATGCGGATGAAAAAAGAATTTGGCACCTTGGTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAAt                            >  2:7400006/1‑140 (MQ=255)
                                                                                                            ccAGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATc                           <  2:1367290/140‑1 (MQ=255)
                                                                                                              aGCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCAc                         >  2:7552141/1‑140 (MQ=255)
                                                                                                               gCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGACGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACg                        >  2:561455/1‑140 (MQ=255)
                                                                                                               gCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACg                        >  2:1950646/1‑140 (MQ=255)
                                                                                                               gCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACg                        >  2:6090939/1‑140 (MQ=255)
                                                                                                               gCTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACg                        >  1:2177269/1‑140 (MQ=255)
                                                                                                                cTGCCCGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGt                       >  2:6431155/1‑140 (MQ=255)
                                                                                                                    ccGAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAg                   <  2:2783490/140‑1 (MQ=255)
                                                                                                                    ccGAAGAATCGGCTCGTGCTCGCCCAGTATGAAGCGTTTAGCTCTGAAAATCGCTGTATTAGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAg                   <  2:4125026/140‑1 (MQ=255)
                                                                                                                      gAAGCATCGGCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCt                 <  1:1475604/140‑1 (MQ=255)
                                                                                                                               gcgccTGCTCGCCCCGTATGAAGAGTGTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAAGTTGGCACCTTGTTTCGTATAAAGTACAAACCCCGCTTTGCCCACCACTCGCAAAACACGTTCACCTGACTTcagc        <  1:691726/137‑1 (MQ=255)
                                                                                                                               gCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCTGACTTcagc        <  2:4917165/140‑1 (MQ=255)
                                                                                                                               gCTCCTGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCTGACTTcagc        <  2:2848958/140‑1 (MQ=255)
                                                                                                                                    tGCTCGCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCTGACTTCAGCAGGtt   >  1:162359/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TTTTCAATCCCAATCACAGAACCCGTTTCACCTACTGCCATACTTGCGATAATGGCATCCGAACCGAGCCCGAGTGTACAATCTAAAAACGTGTCCCCTTCCGATAAACCAGCTGCCCGAAGCATCGGCTCCTGCTCCCCCCGTATGAAGCGTTTAGCTCTGAACATCGCTGTATTCGGATGAAAAAAGAATTTGGCACCTTGTTTCGTATAAAGTTCAAACCGCTCTTTGCCGACCACTAGCAAATCACGTTCAGCTGACTTCAGCAGGTTT  >  NZ_CP034943/2169062‑2169334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: