Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,237,227 C→A 6.0% intergenic (‑334/+15) EO946_RS11350 ← / ← aroA tetratricopeptide repeat protein/3‑phosphoshikimate 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,237,2270CA 6.0% 1065.9 / 15.8 401intergenic (‑334/+15)EO946_RS11350/aroAtetratricopeptide repeat protein/3‑phosphoshikimate 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base C (213/146);  new base A (34/2);  total (252/149)
Fisher's exact test for biased strand distribution p-value = 7.13e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 401 total aligned reads displayed.

GTAAAACAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATT  >  NZ_CP034943/2237094‑2237365
                                                                                                                                     |                                                                                                                                          
gTAAAACAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAATCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAg                                                                                                                                      >  2:6374660/1‑140 (MQ=255)
gTAAAACAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAg                                                                                                                                      >  2:238735/1‑140 (MQ=255)
gTAAAACAGCCCTCTGACCTGCCGTTGTGTATAAAGCGCATCCCCCCGTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTGTGTTTTTTTGAGTCGGATTATGATTCTCACAGGCATGCTGCCCAAAAAAGCCTGacg                                                                                                                                      >  2:6170661/1‑138 (MQ=255)
    aaCAGCCCTCTGACCTGTCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTTTTTTATCTCTATAAAACAACCGAAGTTTTTTTTCCGCGGGTTATGATTGTCACAGGCATTGTGCCCAAAAAAAACTGAAGGaaa                                                                                                                                  >  1:2258838/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTGTTTTCTCTCTATAAGACAAGCTGTGTTTTTTTTCATCGGATTATGATTATAACAGGCAGTCTGAAAAAAAAATACTGAAGGaaa                                                                                                                                  >  2:6440671/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGaaa                                                                                                                                  <  2:5443864/140‑1 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATACAGAATGaaa                                                                                                                                  >  1:4995589/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTATATAAGGAAAGCTATGTTTTTTTGCCTAGGATTATAATTCTCAAACGCATTCTGCACAAAAAAAACAGAAGGaaa                                                                                                                                  >  1:868895/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTATATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGaaa                                                                                                                                  >  2:2431698/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTATTTTGTCTCTATAAGACAAGATATTTTTTTTTGCCTCGGATTATTATTCTCAAAGGCATTCTGCACAAAAAAAAAAGAAGGaaa                                                                                                                                  >  1:5345981/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTATTTTCTCTCTATAAGACAAGCGATGTTTTTTTGCCTGGGCTTATGATTGTCACATGCAATCTGCACAAAAAATACAGAAGGaaa                                                                                                                                  >  1:3511514/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTGTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGaaa                                                                                                                                  <  1:5862325/140‑1 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTCTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGaaa                                                                                                                                  >  2:5973659/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCGTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGaaa                                                                                                                                  >  1:1805117/1‑140 (MQ=255)
    aaCAGCCCTCTGACCTGCAGTTTTGTATAAAGCGCATCCCCCCTTTTAGTTTTTTATTTTCTATCTATAAGACAATCTATGTTTTTTTTCCTCGGATTATGATTCTCAAAGGCATTCTTCACAAAAAATCCTGAAGGaaa                                                                                                                                  >  2:772416/1‑140 (MQ=255)
      cAGCCCTCTGACCTGCCGTTTTTTATAAAGCGCATCCCCCCTTTTCGTGTTTTGTTTTGTCGAGATAAGACCAGCTTTGTTTTTTTTCCTGGGGTTTTGATTGTGACAGGCGTTCTGGCCAAAAAATCCTGAAGGAAAAt                                                                                                                                >  1:141224/1‑140 (MQ=255)
      cAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCGCCTGTTGGTTTTTTCTTTTCTCTGTATAAGACAAGCTATGTTTGTTTGCCTCGGATTCTGATTCTCACAGGGATTCTGCACAATAAATCCTggaggagact                                                                                                                                >  1:2767219/1‑136 (MQ=255)
      cAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGGGATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAAt                                                                                                                                >  1:3549157/1‑140 (MQ=255)
      cAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAAt                                                                                                                                >  2:3421963/1‑140 (MQ=255)
      cAGCCCTCTGACCTGCCGGTTTGTATAAAGCGCATCCCCCCTTGTCGTTTTTTCTTGTCTAGCGATAAGACAATCAATGGTTTGTTGCCTCGGCTTATGAGTCTCCAAGCCATTCAGCACCAAAAATCCTGAGTGAAAAt                                                                                                                                >  2:7060204/1‑140 (MQ=255)
         cccTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTGTTTTGTCTCTATAAGACAAGCTCTGTTTTTTTTCCTCGGATTATGATTGTCACAGGCATTATGCACAAAAAATACTGAAGGAAAATCtt                                                                                                                             >  1:5760286/1‑140 (MQ=255)
         cccTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTGTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGCTTATGATTATCACAGGCATTATTCACAAAAAATCCTGAAGGaaaagatt                                                                                                                             >  1:5987236/1‑136 (MQ=255)
         cccTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACCAGCGATGTTTTTTTTCCTCGGCTTATGATTATCACAGGCATTCTGCACAAAAAATACAGAAGGAAAATCtt                                                                                                                             >  1:7515937/1‑140 (MQ=255)
         cccTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTTCCTCGGATTATGGTTGTCACAGGCATTCTGCACAAAAAATACTGAAGGAAAATCtt                                                                                                                             >  1:3264588/1‑140 (MQ=255)
         cccTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTGTCAAAGGCATTCTGCACAAAAAATCCTGAATGAAAAtagt                                                                                                                             >  1:2359377/1‑137 (MQ=255)
           ctctGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTGTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCa                                                                                                                           >  2:311390/1‑140 (MQ=255)
               gACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTGTTTTGTCTCGATAATACAAGCGATTTTTTTTTTCCTCGGATTATGATTCTCAAACGCATTATGCACAAAAAATCCTGAAGGAAAATATTGAGGAt                                                                                                                       >  1:1280166/1‑140 (MQ=255)
               gACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGAt                                                                                                                       <  1:811563/140‑1 (MQ=255)
               gACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGAt                                                                                                                       <  1:3927878/140‑1 (MQ=255)
                     ccGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCGCTCTATAAGACCAGCGATTTTTTTTTTCCGCGGCTTATGTATATCACAGGGATTCTGCACAAAAAATGCAGCAGGAAAATCTTGCGGAtttgttt                                                                                                                 >  1:6467381/1‑140 (MQ=255)
                     ccGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTATTTTCTCTCTATAAGCCAAGGTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCAGTATGCACAAAAAATCCTGATGGAAAATCTTGAGGagtgggtt                                                                                                                 >  1:3132533/1‑133 (MQ=255)
                      cGTTTTGTACACAGCGCATCCCCCCCTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGATTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGAtttgtttg                                                                                                                <  2:7215720/140‑1 (MQ=255)
                         tttGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGaaa                                                                                                             <  1:5236255/140‑1 (MQ=255)
                         caggTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAATCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGaaa                                                                                                             <  2:6063474/137‑1 (MQ=255)
                          ttGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAg                                                                                                            <  2:1811911/140‑1 (MQ=255)
                           tGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGc                                                                                                           <  1:2492990/140‑1 (MQ=255)
                                  aGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTa                                                                                                    >  2:3512943/1‑140 (MQ=255)
                                       aTCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTTCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGc                                                                                               >  2:257890/1‑140 (MQ=255)
                                       aTCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGc                                                                                               >  2:127975/1‑140 (MQ=255)
                                       aTCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGc                                                                                               >  2:7590384/1‑140 (MQ=255)
                                       aTCCCCCCTTTTCGTGTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGGCTCGGATTAGGATTGTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTAAGGAGGTGTTTGATATCTTATTTAAATGc                                                                                               >  1:7313035/1‑140 (MQ=255)
                                               ttttCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGTATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGaaaaa                                                                                       <  2:5217704/140‑1 (MQ=255)
                                                 ttCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAgg                                                                                     >  2:6262090/1‑140 (MQ=255)
                                                  tCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGt                                                                                    <  1:3443496/140‑1 (MQ=255)
                                                   cGTTTTTTCTTTTCTCTCTATAATACAACCTATGTTTTTTTGCCGCGGATTATGATTATCACAGGCATTCTGCACAAAAAATCCTTAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGtt                                                                                   >  1:1636027/1‑140 (MQ=255)
                                                          tcttttctCTCTCTAAGACAAGCTATGTTTTTGTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAATATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAg                                                                            <  2:2645108/140‑1 (MQ=255)
                                                           cttttctCTCTATAATACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTTCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGa                                                                           <  1:3639592/140‑1 (MQ=255)
                                                           cttttctCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGa                                                                           <  1:1014212/140‑1 (MQ=255)
                                                           cttttctCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGa                                                                           <  2:3042278/140‑1 (MQ=255)
                                                           cttttctCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGa                                                                           <  1:7430683/140‑1 (MQ=255)
                                                                   tctATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGaa                                                                   <  1:4664313/140‑1 (MQ=255)
                                                                        aaGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCa                                                              >  2:2776585/1‑140 (MQ=255)
                                                                        aaGACAAGCTATGTCTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCa                                                              <  2:1090463/140‑1 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACTTGAATGGCAtctgtct                                                       >  1:6066720/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGACTGGCAtctgtct                                                       >  2:565639/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:1261063/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:3207836/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  2:3773783/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:4427896/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:2592929/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:6268834/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:6288744/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:6332190/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:2093134/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:6781196/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  2:1024287/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:6883843/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:1874369/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGAGTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:472724/1‑140 (MQ=255)
                                                                               gCTATGTTTTTTTGCCTCGGAGTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  1:4102421/1‑140 (MQ=255)
                                                                               gCTATGTTTGTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCAtctgtct                                                       >  2:2060414/1‑140 (MQ=255)
                                                                                  aTGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCt                                                    <  2:6781196/140‑1 (MQ=255)
                                                                                               tCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTgg                                       <  1:4250886/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  2:4726306/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  2:4995589/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  2:4170236/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  1:7668375/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  1:2996110/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  2:1312774/140‑1 (MQ=255)
                                                                                                    ttATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGATTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  1:7191221/140‑1 (MQ=255)
                                                                                                            ctcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTtata                          >  1:2675059/1‑140 (MQ=255)
                                                                                                            ctcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTtata                          >  1:3337127/1‑140 (MQ=255)
                                                                                                            ctcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTtata                          >  1:4387454/1‑140 (MQ=255)
                                                                                                            ctcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTtata                          >  1:7037175/1‑140 (MQ=255)
                                                                                                             tcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATac                         >  1:5502533/1‑140 (MQ=255)
                                                                                                             tcACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATac                         >  1:6617301/1‑140 (MQ=255)
                                                                                                              cacaGGCATTCTGCACAAAATATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAACAGGTTGGTTAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATaca                        <  2:1566879/140‑1 (MQ=255)
                                                                                                              cacaGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATaca                        <  1:6951618/140‑1 (MQ=255)
                                                                                                              cacaGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATaca                        <  2:3189322/140‑1 (MQ=255)
                                                                                                              cacaGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATaca                        <  1:2416918/140‑1 (MQ=255)
                                                                                                               acaGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATacac                       >  2:3325342/1‑140 (MQ=255)
                                                                                                                 aGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGa                     >  2:7491251/1‑140 (MQ=255)
                                                                                                                   gCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAg                   <  1:6099200/140‑1 (MQ=255)
                                                                                                                    cATTCGGCACAAAAAATCCTGACGGAAAATCGTCAGGATTTGTTTGAAAGCTTATTTAAATCCTCGAAAAAGGTTGGATAAGAAACCTGAAGGGCATCTGTCTGCTCACTTTCAATTGGCTCTTCAGTTATACACGACGc                  <  2:229664/140‑1 (MQ=255)
                                                                                                                     aTTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCg                 <  2:5324794/140‑1 (MQ=255)
                                                                                                                       tCTGCACAAAAAATCCTGAAGGAAAATCTTCCGGATTTGGTTGAAAGCTTATTTCAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCGTCAGTTATACACGAAGCGAt               >  2:3593979/1‑140 (MQ=255)
                                                                                                                          gCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATAcc            <  1:6296901/140‑1 (MQ=255)
                                                                                                                          gCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATAcc            <  1:1106203/140‑1 (MQ=255)
                                                                                                                               aaaaaTCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  >  2:4588698/1‑113 (MQ=255)
                                                                                                                               aaaaaTCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCtt                                  <  1:4588698/113‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                          
GTAAAACAGCCCTCTGACCTGCCGTTTTGTATAAAGCGCATCCCCCCTTTTCGTTTTTTCTTTTCTCTCTATAAGACAAGCTATGTTTTTTTGCCTCGGATTATGATTCTCACAGGCATTCTGCACAAAAAATCCTGAAGGAAAATCTTCAGGATTTGTTTGAAAGCTTATTTAAATGCTCGAAAAAGGTTGGATAAGAAACGTGAATGGCATCTGTCTGCTCAATTTCAATTGGCTCTTCAGTTATACACGAAGCGATACCAAGCATCATT  >  NZ_CP034943/2237094‑2237365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: