Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,270,052 G→C 14.4% L43V (CTT→GTT)  prsW ← glutamic‑type intramembrane protease PrsW

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,270,0520GC14.4% 640.9 / 24.4 342L43V (CTT→GTT) prsWglutamic‑type intramembrane protease PrsW
Reads supporting (aligned to +/- strand):  ref base G (124/146);  new base C (2/54);  total (130/212)
Fisher's exact test for biased strand distribution p-value = 7.19e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 343 total aligned reads displayed.

TAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCT  >  NZ_CP034943/2269916‑2270190
                                                                                                                                        |                                                                                                                                          
tAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTACGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGaac                                                                                                                                         <  2:5134008/140‑1 (MQ=255)
      cTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTTAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAAcccc                                                                                                                                   <  2:3094509/140‑1 (MQ=255)
      cTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAAcccc                                                                                                                                   <  2:6574803/140‑1 (MQ=255)
      cTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAAcccc                                                                                                                                   <  2:2935929/140‑1 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACTAAAAAGCTTCCGCCTCCCACATTATCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACACCTAg                                                                                                                                >  2:4189799/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGTAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGTGAAATACAAGAACACCCCCTAg                                                                                                                                >  2:1580682/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGTGAAATACAAGAACAACACCTAg                                                                                                                                >  2:2637075/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAGTACAAGAACAACCCCTAg                                                                                                                                >  1:2019482/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAATAAAAACCCCTAg                                                                                                                                >  2:5569431/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:479384/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  2:1230819/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:1028972/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:919784/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  2:345444/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:4955619/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:378602/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:3628589/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:1918389/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:2413334/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:2789229/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:1351210/1‑140 (MQ=255)
         aCCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAg                                                                                                                                >  1:3006702/1‑140 (MQ=255)
         aCCATCACAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCATCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACACGAAAAACCCCTAg                                                                                                                                >  2:2025773/1‑140 (MQ=255)
                   aaaaaCATTTTAATGATTCCTCCAAAAACCCTGAACATAAAAAAGACACCAAAACGATTCCGCCGCCCCAATTCTATTTTTAAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGAc                                                                                                                      <  2:6797346/139‑1 (MQ=255)
                     aaCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCg                                                                                                                    >  2:1026582/1‑140 (MQ=255)
                      aCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATGCAAGAACAACCCCTAGAAAAAACGACCGa                                                                                                                   >  1:681567/1‑140 (MQ=255)
                      aCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGa                                                                                                                   >  2:5374895/1‑140 (MQ=255)
                      aCCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCGAGAAAAAACGACCGa                                                                                                                   >  2:5491405/1‑140 (MQ=255)
                         tttttAATTATTACTCCAAAACCCCTTAAGATAAAAAACAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:3795917/139‑1 (MQ=255)
                         aTTTTATTTCTTCCTCCAACAACGCTGAACATAAAAAAGACACCAAAAAGCTTCCGCCTCCCCAATTCTCTTTTTCAACCCCGTAGTGGATAAACTTTGTGGGAAATAACAGAACAACCCGTACAAAACACGACCGAAGc                                                                                                                <  1:4501247/140‑1 (MQ=255)
                         aTTTTATTTATTCCTACAAAAACCCTTAAGTTAAAAAAGAAACCAAACAGCTTCCGCCTCCCACATTCTCTTTTTAATTCACTTACTGGATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:5266152/140‑1 (MQ=255)
                         aTTTTAATTATTCCTCCAAAAACCCTGAATAAAAAAAATAAACGAAAAAGCTTCCGCCTCCCAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:3338107/140‑1 (MQ=255)
                         aTTTTAATTATTCATCCAAAAACCCTGAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTAAAGCACTTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:6360494/140‑1 (MQ=255)
                         aTTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCAAAATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:7357067/140‑1 (MQ=255)
                         aTTTTAATGATTCCTCCAAAAACACTCAAGATAAAAAACAAACCAAAAAGCTGCCGCCTCCCACATTCTATTTTTCAAGCACGTACTGCATAAACATTGTGTCAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGc                                                                                                                <  2:4411956/140‑1 (MQ=255)
                         aTTTTAATGATTCCACAAAAACCCCTCAATATAAAAAACAAACCAAAACGCTTCCGCCTCCCACATTCTTTTTTTCAAGCCCGTACTGGATAAAAATTGTGGTAAATACACCAACACCCCCCACAAAAAACGACCGAAGc                                                                                                                <  1:1662875/140‑1 (MQ=255)
                          ttttAATGATTCCACCAAAAACCCTTAATATAAAAAAGAAACGAAAAAGGTTCCGCCACCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGTAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCa                                                                                                               <  2:1847285/140‑1 (MQ=255)
                           tttAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCAc                                                                                                              >  1:7532587/1‑140 (MQ=255)
                            ttAATTATTCCTCAAAAAACCCTCAATATAAAAAATAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATCCAAGAACAACCCCTACAAAAAACGACCGAAGCAcc                                                                                                             <  1:4137891/140‑1 (MQ=255)
                             ttattaTTCCACAAAAAACCCTGAACATAAAAAAGAACCGAAAAAGCTTCCGCCCCCAACATTCTCTTTTTCAAGCACTTACTGGATAAACATTGTGGGAAATACAACACCAACCCCGACAAAAAACGACCGAAGCACCa                                                                                                            <  1:7342323/135‑1 (MQ=255)
                              aaTTATTCCTCAAAAACCCCGGAAGATAAAAAAGAAACCAAACAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACACGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                           <  2:3237308/140‑1 (MQ=255)
                              aaTTATTCATCAAAAAACCCTGAAGATAAAAAAGAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACTTTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                           <  2:3554578/140‑1 (MQ=255)
                              aaTGATTCCTCCAACAACCCTGAAGATAAAAAATAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                           <  2:4490530/140‑1 (MQ=255)
                              aaTGATTCATCCAAAAACCCTGAAGATAAAAAACAAACGAAAACGCTGCGGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCat                                                                                                           <  2:2791854/140‑1 (MQ=255)
                                   ttCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTa                                                                                                      <  2:1525806/140‑1 (MQ=255)
                                    tcctccAAAAACCCTTAATATAAAAAACAAACGAAAAAGATTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                     <  2:1681360/140‑1 (MQ=255)
                                    tcctccAAAAACCCTGAAGATAAAAAAGAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                     <  2:1266005/140‑1 (MQ=255)
                                    tcctaaaaaaaCCCTGAAGATAAAAAAGAAACGAAAAAGGTTCCGCCTCCCACTTGCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTAc                                                                                                     <  2:7266691/134‑1 (MQ=255)
                                     cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                                                                                    >  2:108401/1‑140 (MQ=255)
                                     cctccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACa                                                                                                    >  2:2070140/1‑140 (MQ=255)
                                        ccAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGt                                                                                                 >  1:6706984/1‑140 (MQ=255)
                                         cAAAACCCCTGAAGATAAAAAACAAACCAAACAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACGGTATAAACATTGTGGGAAATACAACAACAACCCCCAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  2:6434752/140‑1 (MQ=255)
                                         cAAAACCCCTCAACATAAAAAACAAACCAAACCGCTTCCGCCCCCCACATTCTCTTTTTCACGCACGTACTGGATAAACTTTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:6978945/140‑1 (MQ=255)
                                         cAAAAACCCTTAAGATAAAAATGAAACGAAAACGCTGCCGCTTCCCACTTTATATTTTTCAAGCACGTCCTGTATAAATTTTGTGGTATCTCCAACACCAACCCGTCGAACAAACGACCGAAGCACCATATGTAGAGGtt                                                                                                <  2:627052/140‑1 (MQ=255)
                                         cAAAAACCCTGAACATAAAAAAGAAACCAAAAACCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  2:2663331/140‑1 (MQ=255)
                                         cAAAAACCCTCAACATAAAAAACAAACCAAAAAGGTTCCGCCTCCCACATTCTCTTTTACACGCACGTACTGGATAAACTTTGTGGTAAATACAACAACAACCCCAACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:7130630/140‑1 (MQ=255)
                                         cAAAAACCATCAAAATAAAAAACACACCAAAAAACTGCCCCCCCCCAAATACTCTTTTACATGCACGTACTGTATAAAAATTGTGGGAAATACACTAACAACCCCTAAAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:5082464/140‑1 (MQ=255)
                                         aaaaacCCCTAAACAAAAAAAACAAACCAAAAAGCTTCCGCCTCCCAAATTATATTTTTAAAGCACTTACTGTATAAACATTGTGGTAAATACAACAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:6283801/139‑1 (MQ=255)
                                         aaaaaaCCCTGATTAAAAAAAACAAACGAAAAAGCGGCCGCATCCCAAATTATTTTTTTAAACCACGTTCTGCATAAACATTGTGGTAAATACAAGAACAACCCATCGAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:4286462/139‑1 (MQ=255)
                                         aaaaaaCCCTCAATAAAAAAAAAAAACCAAAAAGCTCCCGCCCCCCAAATTATATTTTTAAAGCAGGTACTGGATAAACTTTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGtt                                                                                                <  1:4473859/139‑1 (MQ=255)
                                              ccccTTAATATAAAAAAAAAACCAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGTATAAACATTGTGGCAAATACAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                           <  1:5547597/139‑1 (MQ=255)
                                              ccccTCACGATAAACAACAAACGAAAACCCGTCCGCCTCCCACAGACGCTTTTTCACGCAGGTACTGGATAAACTTTGTGGGAAATACACCAACACCCCCTACAAAAAACGACCGACGCACCATATGTACAGGTTCATTa                                                                                           <  1:81774/139‑1 (MQ=255)
                                              acacTGAATATAAAAAAGAAACGAAAAAGATTCCGCCTCACACATTCTATTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTa                                                                                           <  2:7589064/137‑1 (MQ=255)
                                                  tGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCAt                                                                                       >  2:1988792/1‑140 (MQ=255)
                                                           aaaaGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCtt                                                                              >  1:5650949/1‑140 (MQ=255)
                                                              aGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTATta                                                                           >  1:5134970/1‑140 (MQ=255)
                                                                 aaCGAAAAAGCTTCCGCCTCCCACTTTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaat                                                                        <  2:855320/140‑1 (MQ=255)
                                                                 aaCGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAAGACAAGAACAACCCCTAGAAAAAACGACAGAAGCACCATAAGTACAGGTTCATTATCATAATGATCTTTtaaat                                                                        >  1:7647261/1‑140 (MQ=255)
                                                                    caaaaaGCTTCCGCCTCCCAAATTCTCTTTTTAAACCACGTACTGCATAAACTTTGTGGTAAATACAACAACACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTtaaataaa                                                                     <  1:720377/139‑1 (MQ=255)
                                                                      aaaaCCTTCCGCCTCCCACATCCTCTTTTTAAAGCACGTACTGCATAAACATTGTGTAAAATACAACACCACCCCCAACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGAACTTTTaaataaaaa                                                                   <  1:342964/140‑1 (MQ=255)
                                                                              ccGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACACCCCCTAGAAAAAACGACCGAAGCACCATATGGACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTaa                                                           >  2:473541/1‑140 (MQ=255)
                                                                                    ccaacaTTTTCTTTTACACCCACGTACTTTATAAACAGTGTGGTAAATAAAAGAACAACCCAAACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAACAATAACTTAACAgcgc                                                     <  1:2861224/137‑1 (MQ=255)
                                                                                    ccaaaaTTCTCTTTTTCAATCACTTACTTTATAAACTTTGTGGGAAATACAATAACAACCCATACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGACCTTTTAAATAAAAATAACTTAACAgcgc                                                     <  1:2197243/135‑1 (MQ=255)
                                                                                          ttCTCTTTTTCAAGCACTTACTGTATAAACTTTGTGTGAAATACAAGAAAAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGcc                                               <  2:937691/140‑1 (MQ=255)
                                                                                                ttttCATGATCGTATTGTAAAAACTTTTTGGTAAATACAACAACACCCCATACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                         <  2:7645080/140‑1 (MQ=255)
                                                                                                ttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                         >  1:979271/1‑140 (MQ=255)
                                                                                                ttttCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGAGGCCGGGAGc                                         <  2:1335422/140‑1 (MQ=255)
                                                                                                ttttCAAGAAGGTACTGGATAAACTTTGTGGGAAATCCAAGAAAACCCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGc                                         <  1:6079011/140‑1 (MQ=255)
                                                                                                   tCAAGCACGTACTGGATAAACATTGTGGGAAATCCAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGAGGCCGGGAGCAAt                                      <  2:2359762/140‑1 (MQ=255)
                                                                                                   tCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                      <  2:4570927/140‑1 (MQ=255)
                                                                                                   tCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                      <  2:1395775/140‑1 (MQ=255)
                                                                                                   tCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAAt                                      <  2:6992782/140‑1 (MQ=255)
                                                                                                       gCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCt                                  >  1:2336469/1‑140 (MQ=255)
                                                                                                         aCGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGc                                >  2:7205709/1‑140 (MQ=255)
                                                                                                           gTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTCAATAAAAATAACTTAACAGCGCGATGCCGGGAGCACTGCATGCag                              >  2:1118675/1‑140 (MQ=255)
                                                                                                            tACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCaga                             >  2:1344851/1‑140 (MQ=255)
                                                                                                               tgtaTAAACTTTGTGGGAAATAAAAGAACAACCCATAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgat                          <  2:6885841/137‑1 (MQ=255)
                                                                                                               tGGATAAACAGTGTGGTAAATACAACAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAgat                          <  2:6049131/140‑1 (MQ=255)
                                                                                                                     aaCATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGc                    >  1:4620654/1‑140 (MQ=255)
                                                                                                                      aCATTGTGTGAAATAAAAGAACAACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                   <  1:3220704/140‑1 (MQ=255)
                                                                                                                      aCATTGTGGGAAATACCAGAACAACCCCGAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACCGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                   <  2:3871333/140‑1 (MQ=255)
                                                                                                                      aCATTGTGGGAAATACAAGAACAACCCCTAGAACACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCa                   <  2:4204367/140‑1 (MQ=255)
                                                                                                                           gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATAGTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAATCAt              >  2:4272299/1‑140 (MQ=255)
                                                                                                                           gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt              >  2:5071861/1‑140 (MQ=255)
                                                                                                                           gtgGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACAt              >  1:1440898/1‑140 (MQ=255)
                                                                                                                                aaaTACAAGAACAACCCCTATAAAACACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGc         <  2:2447875/140‑1 (MQ=255)
                                                                                                                                 aaTACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCa        >  2:7067595/1‑140 (MQ=255)
                                                                                                                                   aacaacaacaACCCCTACAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAAc      <  1:6017398/139‑1 (MQ=255)
                                                                                                                                      aaGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctc   <  2:6706984/140‑1 (MQ=255)
                                                                                                                                       aGACCAACCCCTAGAAAAAACGACAGAAGCACCCTATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACACCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACctct  <  1:1752722/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TAAACACTGACCATCAGAATAAACCATTTTAATGATTCCTCCAAAAACCCTGAAGATAAAAAAGAAACGAAAAAGCTTCCGCCTCCCACATTCTCTTTTTCAAGCACGTACTGGATAAACATTGTGGGAAATACAAGAACAACCCCTAGAAAAAACGACCGAAGCACCATATGTACAGGTTCATTATCATACTGATCTTTTAAATAAAAATAACTTAACAGCGCGATGCCGGGAGCAATTCCTGCAGAGATGATTGCAAACATCAGGCAACCTCT  >  NZ_CP034943/2269916‑2270190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: