Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,026 T→G 19.9% K367T (AAG→ACG)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,0260TG19.9% 613.3 / 49.4 321K367T (AAG→ACG) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (111/134);  new base G (74/2);  total (185/136)
Fisher's exact test for biased strand distribution p-value = 6.92e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

CTGCGCCAGCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGC  >  NZ_CP034943/2386887‑2387165
                                                                                                                                           |                                                                                                                                           
cTGCGCCAGCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCt                                                                                                                                             >  1:3038076/1‑140 (MQ=255)
   cgcCAGCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTa                                                                                                                                          >  2:3869814/1‑140 (MQ=255)
   cgcCAGCTACCATGATTTGTTTCATTTCCATTCAAAGCCCCTGTGTCGTCTGTTTTAATGAACTTGCACTATGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCGCCCCGTCGCTTTa                                                                                                                                          >  2:4460256/1‑140 (MQ=255)
   cgcCAGCTACCATGAGTTGTTTCATTTCCATTCAAATCCCCTTTTTCTTCTGTTTTAATGAACTTGCACTAATACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCGACACCCAGTCCCCCTCCCCGGCGCtgtt                                                                                                                                          >  1:2449126/1‑137 (MQ=255)
        gCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAttag                                                                                                                                     >  1:1697922/1‑138 (MQ=255)
        gCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCg                                                                                                                                     >  1:2652809/1‑140 (MQ=255)
        gCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTaaatag                                                                                                                                     >  1:204668/1‑136 (MQ=255)
        gCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCg                                                                                                                                     >  1:3928442/1‑140 (MQ=255)
        gCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCg                                                                                                                                     >  2:6052536/1‑140 (MQ=255)
        gCTACCATGATTTGTTTAATTTCCATTCAAATACCCTTTATCGTATGTTTTAATTAACTTGCACTAAGACGGCGTCACCCTGAGCTCAACAGCTCCAAATGGCGGCAACAACAAGTCCCCCTCCCCGTCGCTTTaaatac                                                                                                                                     >  1:4682804/1‑136 (MQ=255)
            ccATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTaaa                                                                                                                                 <  1:5388834/140‑1 (MQ=255)
             cATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAATACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGACCCCCTCCCCGTCGCTTGAATTAGTAAAc                                                                                                                                >  1:4542758/1‑140 (MQ=255)
             cATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTaaaa                                                                                                                                >  1:677901/1‑139 (MQ=255)
               tGATTTGTTTCATTTCCCTTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAAGTCGTAAACaa                                                                                                                              >  2:1382650/1‑140 (MQ=255)
               tGATTTGTTTCATTTCCATTCACATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGGAAAcca                                                                                                                              >  2:2460518/1‑138 (MQ=255)
               tGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACaa                                                                                                                              <  2:6291394/140‑1 (MQ=255)
               tGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTACTTACTAAACaa                                                                                                                              >  2:6760503/1‑140 (MQ=255)
                  tttGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAgtg                                                                                                                           <  2:3045014/140‑1 (MQ=255)
                    tGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTc                                                                                                                         <  2:5275035/140‑1 (MQ=255)
                    tGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTc                                                                                                                         <  2:6459348/140‑1 (MQ=255)
                    tGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAACAAGTGTc                                                                                                                         >  2:1280712/1‑140 (MQ=255)
                    tGTTTCATTTCCATTCAAATCCCCTGTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTc                                                                                                                         >  2:1688115/1‑140 (MQ=255)
                    tGTTTCATGTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTc                                                                                                                         <  2:6406741/140‑1 (MQ=255)
                      tttCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTGTAATTCGTAAACAAGTGTcgt                                                                                                                       >  1:3101583/1‑138 (MQ=255)
                      tttCATTTCCATGCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACaatggtaat                                                                                                                       >  1:7226545/1‑133 (MQ=255)
                      tttCATTTACATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCAt                                                                                                                       <  1:6197172/140‑1 (MQ=255)
                       ttCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATg                                                                                                                      >  2:6793988/1‑140 (MQ=255)
                       ttCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATg                                                                                                                      <  2:2816412/140‑1 (MQ=255)
                       ttCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATg                                                                                                                      <  2:6730522/140‑1 (MQ=255)
                       ttCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACGTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATg                                                                                                                      <  2:1102002/140‑1 (MQ=255)
                       ttCATTTCCATTCAAATCCCCTTGTTCGTCTGTTTTAATGAACTTGCACTACGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTCATg                                                                                                                      <  2:4709735/140‑1 (MQ=255)
                         cATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAg                                                                                                                    <  1:4350872/140‑1 (MQ=255)
                           tttCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTaaa                                                                                                                                 >  2:4916249/1‑125 (MQ=255)
                           tttCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTaaa                                                                                                                                 <  1:4916249/125‑1 (MQ=255)
                           tttCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCAAGAGTa                                                                                                                  >  2:619868/1‑140 (MQ=255)
                           tttCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCCAGAGTa                                                                                                                  >  2:557568/1‑140 (MQ=255)
                               cATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATtct                                                                                                              <  1:4183116/140‑1 (MQ=255)
                                 ttCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCg                                                                                                            <  1:252664/140‑1 (MQ=255)
                                 ctCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCACTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCg                                                                                                            <  1:7641057/139‑1 (MQ=255)
                                  tCAACTCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGc                                                                                                           <  1:2947867/140‑1 (MQ=255)
                                  tCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGc                                                                                                           <  1:4340003/140‑1 (MQ=255)
                                  tCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGc                                                                                                           <  1:6865096/140‑1 (MQ=255)
                                      aTCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTAACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAATAGTAAACAATTCTCAAGAGTATTAACGCACCg                                                                                                       >  2:220571/1‑140 (MQ=255)
                                          ccTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTg                                                                                                   <  1:6393302/140‑1 (MQ=255)
                                                tCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTTTCATGAGTATTCGAGCACCGCTTGCGccca                                                                                             >  2:7420151/1‑138 (MQ=255)
                                                tCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGa                                                                                             <  1:4776480/140‑1 (MQ=255)
                                                 cGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGAt                                                                                            >  2:7071788/1‑140 (MQ=255)
                                                 cGTCTGTTTTAATGAACTTGCACTAAGACGGAGTCACCCTGAGCTGCACCGCTGCAAATGGCCGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAACTGTCATTAGTATTCTCGCACAGCATTCGCCGAt                                                                                            >  2:4187439/1‑140 (MQ=255)
                                                                acgtGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                             <  1:518251/137‑1 (MQ=255)
                                                                aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                             <  2:3390320/140‑1 (MQ=255)
                                                                aCTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGc                                                                             <  1:1917576/140‑1 (MQ=255)
                                                                 cTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCATTAGTATTCTCGCACCGCGTGCCCCGATAGGGTGGCCGAGGGCg                                                                            >  1:1867538/1‑140 (MQ=255)
                                                                  ttGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGa                                                                           <  1:3572256/140‑1 (MQ=255)
                                                                  ttGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGa                                                                           <  1:5304222/140‑1 (MQ=255)
                                                                  gtGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGa                                                                           <  1:2869268/139‑1 (MQ=255)
                                                                        tAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGcac                                                                     <  2:6097203/140‑1 (MQ=255)
                                                                          aGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGCGGGCGCTCGcacca                                                                   >  2:1854197/1‑140 (MQ=255)
                                                                          aGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAGTGTCATGCGTATTATAGCAACGCTTGGGCCGGACGGGTGGCCGAGGGCGATCGgacca                                                                   >  2:2685871/1‑140 (MQ=255)
                                                                              ggCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGt                                                               <  2:7407950/140‑1 (MQ=255)
                                                                               gCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGtt                                                              <  2:4354075/140‑1 (MQ=255)
                                                                               gCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTTAAAAACTGGCATGGGTATTATCGCACCGCGTGCGCCGCACCGGGGGGCGAGGGGGAGCGCACCCCCggg                                                              >  1:3269768/1‑138 (MQ=255)
                                                                                cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCCGTCCCCCTCCCCGTCGCTTGTTGTGGGAAACCCCTGTGATGAGGTGTTTCGCACCGCGGTGGCCGCTCGGGTGGGCGCGGGGGATGGCCCCACCgggt                                                             >  1:6043596/1‑137 (MQ=255)
                                                                                cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTCGTAAACAAGTGTCATGTGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCCATGGCACCACCCttt                                                             >  2:3124136/1‑140 (MQ=255)
                                                                                cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTTATTAGTAAACCAGTGTGATGAGGATTCTCGCACCGCGTGCGCCGCTCGGGGGGGCGCGGGCGAGCGCcccccccggt                                                             >  1:5876612/1‑136 (MQ=255)
                                                                                cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTGGTAAACAAATGTGATGTGTTTTGTCGCACCCCGTGCGCCGCTCGGGGGGGCGGGGGGGATCGCaccacccggt                                                             >  1:498294/1‑136 (MQ=255)
                                                                                cGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAAAAAGTGTCAAGAGTATTGTAGCACCGCTTGCGCCCATCGGGTGGGCGAGGGCGATAGCACCACCGgtt                                                             >  1:1238454/1‑140 (MQ=255)
                                                                                cGTCACCCGGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTATACCAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGCTCGGGTGGCCGGGGGCAATCGCACCACCGgtt                                                             >  2:5417364/1‑140 (MQ=255)
                                                                                          aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTGCTAAAAAACTGTGAATAGTATTCGCGCACCGCTTGCGCCGGTCGGGTGGGCGATGGCGGTCGCACCACCGGTGACCTTTAAtt                                                   >  1:96735/1‑140 (MQ=255)
                                                                                          aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCCTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACtt                                                   >  2:3519599/1‑140 (MQ=255)
                                                                                            cTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAAAAAGTGTCAAGAGTATTCTCGCACCGCTTTCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTAGATTTAAtttt                                                 >  2:7159852/1‑140 (MQ=255)
                                                                                              gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTAGATTTACTTTTTc                                               <  1:7447499/140‑1 (MQ=255)
                                                                                              gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                               <  1:5630399/140‑1 (MQ=255)
                                                                                              gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                               <  2:3994236/140‑1 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAAACAGTGTGATGAGTATTCTCGCACCGCGTGCGCCCAACCGGTGGCCGCCGGCGATCGCAACACCCGTTACATTTACATTTTCa                                              >  2:296776/1‑140 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAGTCGTAAACAAGTGTGATGAGTATTCTCGCACCGCTGGCGCCGATCGGGTGGCCGAGGGCGATCGCCCCCCCGGTTACCTTTAATTTTTCa                                              >  2:7093633/1‑140 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAAATAGTAAAAAAGTGTCATGAGTATTATCGCACCCCGTTCGCCGATCGGGTGGCCGAGGGCGCTCGCACCACCCGGTACATTTACTTTTTCa                                              >  1:3494031/1‑140 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTATAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGCTCGGGGGGCCGCGGGCCGTCCCCCCACCCGTTACCTTTACTTTTTCa                                              >  2:3423360/1‑140 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTGGTAAAAAAATTTGATGAGTGTTGTCGCACCGCGTGCGCCGCGCGGGGGGGCGCGGGGGGGCGCACCCCCCGTTACCTTTACtttttga                                              >  1:1109197/1‑138 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGGAAACAATGGTCAAGAGTATTCTCGCACCCCATGCGCCCATCGGGTGGCCGCGGGCGATCGCAGCACCGTGTAACTTTAATTTTTCa                                              >  2:2867970/1‑140 (MQ=255)
                                                                                               cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCCCTTGAATTCGTAAACAAGTGTCAGGCGAATGCTCGCACCCCATGCGCCGTTCGGGTGGCCGAGGGCGAACGCACCCCCGGTTGCAGTTACTTTTTCa                                              >  2:3695831/1‑140 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTTAAAAAGTGTGAATAGTATTGTCGCACCGCGTGCGCCGGTCGGGGGGGCCCGGGCGCTAGCACCCCCCTTTACATTTAATTTTTAAatata                                         >  1:1409849/1‑139 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTTTCAGGTGTATTATCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGGGATCGCCCCACCGTGTTGAGTTAATTTTTTAAGAtg                                         >  1:7487080/1‑139 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAACTGTCATGAGTATTATGGCACCGCGTGCGCCGAACGGGGGGCCGCGGGCGATCGCCCCCCCGGTTAAATTTAAATTTTGAACATc                                         >  1:5767468/1‑140 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAAAAAATTTCAGGTGGATTCTCGCACCGCGTGCGCCGATCGGGGGGGCGAGGGGGATCGCCCCACCGTTGTCCTTTATTTTTTTAAGAta                                         >  1:3064707/1‑139 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAGTGTCATGAGTAATCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGGTTACATTTACTTTTTCAAGATc                                         >  2:7307200/1‑140 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTGGTAAACAAGTGTCATGAGGATTATCGCACCGCGTGCGCCGCTCGGGGGGCCGAGGGCGCTGGCACCCCCGGGTACATTTAATTTTTGAAGAta                                         >  1:1281302/1‑139 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTGAAGAGTATTCGCGCACCGCTTGCGGCGAGCGGGTGGCCGCGGGCGCGCGCACCCCCGGGGACATTTACTTTTTCAAGATc                                         >  2:6578803/1‑140 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAACTGTCATGAGTATTAGCGCACCCCGTGCGCCCCTCGGGTGGGCGAGGGCGATCGCCCCACCCGTTAGAATTAATTTTTTAAGAta                                         >  1:6983086/1‑139 (MQ=255)
                                                                                                    cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAATGTCATGTGTATTCTCGCACCGCGTGCGCCGATCGGGTGGGCGCGGGCGATCGGCCCACCGGGTACATTTTATTTTTAAAGaag                                         >  1:7033221/1‑138 (MQ=255)
                                                                                                    cTGCAAANGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTAGTAAACAAGTGTGATGGGTAATCTCGCACCCCGTGCGCCGCTCGGGGGGCCGGGGGCGGTCGCACCCCCCGGTTCAATTACTTTTTAAAGATc                                         >  1:6585682/1‑140 (MQ=255)
                                                                                                         aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTGATTAGTTTTGTCGCACCGCGTGCGCCGATCGGGTGGCCGGGGGCGCTCGCACCACCGGGTTCATTTACTTTTTGAAGATAGAAAc                                    >  1:3709953/1‑140 (MQ=255)
                                                                                                                       ccAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTa                      <  1:6317335/140‑1 (MQ=255)
                                                                                                                               ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa              <  2:5822285/140‑1 (MQ=255)
                                                                                                                               ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa              <  2:204668/140‑1 (MQ=255)
                                                                                                                               ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa              <  2:777885/140‑1 (MQ=255)
                                                                                                                                   cccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGATCGCACCACCGTTTAAATTTAATTTTTCAAGATCGAAACCGACCATTTTTTTAAATGTCaaaaaa          >  2:7518830/1‑138 (MQ=255)
                                                                                                                                    ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa         <  2:6926615/140‑1 (MQ=255)
                                                                                                                                    ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa         <  2:3077008/140‑1 (MQ=255)
                                                                                                                                       tCGCTTTAATTCGTAAACAAGTGTGATGAGTATTCTCGCACCGCTTGCGCCCATCCGGTGGCCGAGGGCGATCGCACCACCGTTTAAATTTACTTTTTAAAGATCGCAAACCACGCTTTTTTTACATTTaaaaaaaaaaa      >  2:4264511/1‑137 (MQ=255)
                                                                                                                                       tCGCGTTAAATCGTAAACAAGTGTCAAGAGTATTCTCGCCCCGCTTGCGCCCATAGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTAATTTTTCAAGATCGAAACCGTCCGTTTTTTTTAATGTGAAAAAAACAg      >  2:5134013/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
CTGCGCCAGCTACCATGATTTGTTTCATTTCCATTCAAATCCCCTTTTTCGTCTGTTTTAATGAACTTGCACTAAGACGGCGTCACCCTGAGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGC  >  NZ_CP034943/2386887‑2387165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: