Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,387,114 C→A 8.2% V338L (GTA→TTA)  EO946_RS12195 ← acetyl‑CoA C‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,387,1140CA 8.2% 976.6 / 18.9 383V338L (GTA→TTA) EO946_RS12195acetyl‑CoA C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (173/160);  new base A (43/2);  total (220/163)
Fisher's exact test for biased strand distribution p-value = 1.29e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 385 total aligned reads displayed.

AGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGCT  >  NZ_CP034943/2386977‑2387253
                                                                                                                                         |                                                                                                                                           
aGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTAGTAAACAAGTGTGATGAGGATTATCGCACCCCTTGCGCCCCTCGGGGGGCCGCGGGCGGGCGGACCCCCCGGTACATTTAAtt                                                                                                                                           >  1:2626959/1‑140 (MQ=255)
    gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                                                                                                                       <  1:471351/140‑1 (MQ=255)
    gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                                                                                                                       <  2:677901/140‑1 (MQ=255)
    gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                                                                                                                       <  1:1991719/140‑1 (MQ=255)
    gCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTc                                                                                                                                       <  1:5630399/140‑1 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTGTACGTCGTAAACCAGGGTCATGATTATTCGCGCACCGCTTGCGCCGAGCGGGTGGCCGAGGGCGATCGCCCCACCGTTTAACTTTTCTTTTTCa                                                                                                                                      >  2:5616055/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTGGTAAAAAAATTTGATGAGTGTTGTCGCACCGCGTGCGCCGCGCGGGGGGGCGCGGGGGGGCGCACCCCCCGTTACCTTTACtttttga                                                                                                                                      >  1:1109197/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCATGAGTATTATCGCACCGCTTGCGCCGATCGGGTGGCCGTGGGCGATCGCAACACCGGTTACATTTACTTTTTCa                                                                                                                                      >  2:3380112/1‑140 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAAAAAGTGTGATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGTTCGCACCACCGGTTACATTTAAtttttaa                                                                                                                                      >  2:6317335/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAACAAGTGTGATTAGTATTGTAGCACCGCGGGCGCCGATCGGGTGGGCGCGGGCGGTCGCACCACCGGTTAGATTTAAtttttaa                                                                                                                                      >  1:2470627/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATGCGTAAAAAACTGTGCTGAGTATTATCGCACCCCGTTCGCCGATCGGGGGGCCGAGGGCGCTCCCACCAACGTGTACAATTAAattttaa                                                                                                                                      >  1:4311218/1‑138 (MQ=255)
     cACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCCCTTGAATTCGTAAACAAGTGTCAGGCGAATGCTCGCACCCCATGCGCCGTTCGGGTGGCCGAGGGCGAACGCACCCCCGGTTGCAGTTACTTTTTCa                                                                                                                                      >  2:3695831/1‑140 (MQ=255)
     aaCCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAAAAAATGTCATGGCTATTGTCGCACCCCATGCGCCCAACTGGTGGCGGCCGTCGGACGGACAACCCGTTACATTTAATTTTTCa                                                                                                                                      >  1:5136415/2‑140 (MQ=255)
      aCCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTTAACAAGTGTGATTAGTATTATCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTGTACATTTTATTTTTaaa                                                                                                                                     >  2:5374041/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTTTCAGGTGTATTATCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGGGATCGCCCCACCGTGTTGAGTTAATTTTTTAAGAtg                                                                                                                                 >  1:7487080/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTGGTAAACAAGTGTCATGAGGATTATCGCACCGCGTGCGCCGCTCGGGGGGCCGAGGGCGCTGGCACCCCCGGGTACATTTAATTTTTGAAGAta                                                                                                                                 >  1:1281302/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTTATTGGTAAAAAACTGTCATGAGTATTGTCGCACCCCGTGGGCCGCTCGGGTGGGCGCGGGCGCTCGCACCCCCGGGTGCATTTAATTTTTAAatata                                                                                                                                 >  1:777885/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCATGAGGATTCTCGCACCGCGTGCGCCGCTCGGGGGGCCGCGGGCGCTCGCACCACCGGTTACAGTTACTTTTTCAAGATc                                                                                                                                 >  2:4988944/1‑140 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTAGTAAAAAAGTGTGATGAGTATTGTCGCACCCCGTGCGCCGGGCGGGTGGGCGGGGGCGGTCGGCCCCCCGGTTGCCTTTAATTTTTGAatata                                                                                                                                 >  1:6767204/1‑139 (MQ=255)
          cTGCAAATGGCGGCAACACCCAGTCACCCTCCCCGTCGCTTTAATTAGTACACAATTGTAATGAGTAGTCTCGCACCCCTTGCGCCCATCCGGTGGCCCCGGGGGAATGCACCACCGAGTACATTTAAATTTTCAAGATc                                                                                                                                 >  2:6444084/1‑140 (MQ=255)
               aaTGGCGGCAACACCCAGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTGATTAGTTTTGTCGCACCGCGTGCGCCGATCGGGTGGCCGGGGGCGCTCGCACCACCGGGTTCATTTACTTTTTGAAGATAGAAAc                                                                                                                            >  1:3709953/1‑140 (MQ=255)
                    cggcAACACCCCGTCCCCCTCCCCGTCGCGTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACcgacg                                                                                                                       <  2:4682684/140‑1 (MQ=255)
                            ccccGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGAttttttt                                                                                                               <  2:728540/140‑1 (MQ=255)
                             ccAGTCCCCCTCCCCGTCGCTTTAATTCGGAAACAAGTGTCATGAGTATTCTCGCCCCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTGTTCAAGATCGAAACCGACGATTTTTTTa                                                                                                              <  2:7084196/140‑1 (MQ=255)
                                     ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                                                                      <  2:204668/140‑1 (MQ=255)
                                     ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                                                                      <  2:777885/140‑1 (MQ=255)
                                     ccTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCaa                                                                                                      <  2:3064707/140‑1 (MQ=255)
                                         cccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATAGGGTGGCCGAGGGCGATCGCACCACCGTTTAAATTTAATTTTTCAAGATCGAAACCGACCATTTTTTTAAATGTCaaaaaa                                                                                                  >  2:7518830/1‑138 (MQ=255)
                                          ccGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAaacaa                                                                                                 <  2:3118239/140‑1 (MQ=255)
                                          ccGTCGCTTTAAGTCTTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACAGACGATTTTTTTACATGTCAAaacaa                                                                                                 <  2:3928442/140‑1 (MQ=255)
                                             tCGCTTTAATTCGTAAACAAGTGTGATGAGTATTCTCGCACCGCTTGCGCCCATCCGGTGGCCGAGGGCGATCGCACCACCGTTTAAATTTACTTTTTAAAGATCGCAAACCACGCTTTTTTTACATTTaaaaaaaaaaa                                                                                              >  2:4264511/1‑137 (MQ=255)
                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                         <  1:7321586/140‑1 (MQ=255)
                                                  ttAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCa                                                                                         <  2:5518823/140‑1 (MQ=255)
                                                   tAATTCGTAAAAAAGTGTCATGAGTATTCTCGCACCGCGTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAATATCGAAACCGACGATTTTTTTACATTTCAAAACAAAAGAAGCaa                                                                                        >  2:1634986/1‑140 (MQ=255)
                                                   tAAGTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCaa                                                                                        <  1:6321239/140‑1 (MQ=255)
                                                     aTTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAGCCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAAc                                                                                      <  1:6602324/140‑1 (MQ=255)
                                                        cGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCt                                                                                   <  1:6732063/140‑1 (MQ=255)
                                                          tAAACAAGTGTCATGAGTATTCTCGCACAGCTTGCGCCGATCGGGTGGCCGCGGGCGATCGCACCACCGGTTACATTTACTGTTTCAAGATCGAACCCGACGATTTTTTTACAGGTCAAAACAACAGCAGCCAACGCGTc                                                                                 >  2:4528099/1‑140 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                     <  2:1109197/140‑1 (MQ=255)
                                                                      aTGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCaaa                                                                     <  2:1697922/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:7092946/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:3213630/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    >  1:5906857/1‑140 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:5455926/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:348788/140‑1 (MQ=255)
                                                                       tGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:4474185/140‑1 (MQ=255)
                                                                         aGTATTCTCGCACCGCTTGCGTCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                                                                  >  1:166094/1‑140 (MQ=255)
                                                                         aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACaa                                                                  >  1:7350018/1‑140 (MQ=255)
                                                                         aGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTGTACCGTTACTTTTTCTCGATCGAAACCGACGATTTTTTTACATGTCAAAACAACCGAAGCTAACGCTTCATTTACTTccagcat                                                                  >  1:281603/1‑134 (MQ=255)
                                                                           tATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGt                                                                >  1:226645/1‑140 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCTCACCAACGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATTTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              <  1:6040023/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAacaaca                                                                                               >  2:5734730/1‑107 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  1:6943932/134‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              <  2:7144364/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              >  2:3602307/1‑140 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              <  2:6648248/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              <  1:3376860/140‑1 (MQ=255)
                                                                             ttCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              <  2:6282417/140‑1 (MQ=255)
                                                                              tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa                                                             >  1:6402879/1‑140 (MQ=255)
                                                                              tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa                                                             >  1:645439/1‑140 (MQ=255)
                                                                              tctcGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCaa                                                             >  2:6045948/1‑140 (MQ=255)
                                                                                     ccGCTTGCGCCTATCGGTTGGACGAGTGAGATCTCAAAAACTTTTACATTTAATTTTTCAAGATCTAAACATAAGATTTTTTTACATGTCAAAACAACAGAAGCAAACTTTTAATTTAATTAAATCAAGTCAATATCTTg                                                      <  2:2470627/140‑1 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTc                                                               >  2:4626455/1‑131 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                      <  2:2819864/140‑1 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                      <  1:6531467/140‑1 (MQ=255)
                                                                                     ccGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTg                                                      <  2:1363483/140‑1 (MQ=255)
                                                                                       gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACCTCTAAAAACAACAGAAGCAAACGCTTCATTTACTTCAACCAAGTCAATATATTGGa                                                    <  1:2875581/140‑1 (MQ=255)
                                                                                       gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                    <  1:7184870/140‑1 (MQ=255)
                                                                                       gCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                    <  1:3519599/140‑1 (MQ=255)
                                                                                        cTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGAc                                                   >  1:3802095/1‑140 (MQ=255)
                                                                                         ttGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAACCCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGTACg                                                  >  2:513069/1‑140 (MQ=255)
                                                                                          tGCGCCGATCGGGTGGCCTATGTAGATCGCACAACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACgg                                                 >  2:6988675/1‑140 (MQ=255)
                                                                                                gATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACTCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAAc                                           >  2:935187/1‑140 (MQ=255)
                                                                                                gATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCa                                                              >  1:4833720/1‑121 (MQ=255)
                                                                                                   cgtgTGGCCGAGGGAGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAATCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGt                                        <  2:2930842/137‑1 (MQ=255)
                                                                                                           cGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    >  1:5817799/1‑104 (MQ=255)
                                                                                                           cGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAAc                                                                    <  2:5817799/104‑1 (MQ=255)
                                                                                                              ggtctaTCGCACCACCTTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAgg                             <  1:1452900/135‑1 (MQ=255)
                                                                                                              gggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAgg                             <  1:4279386/140‑1 (MQ=255)
                                                                                                              gggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAgg                             <  1:3124136/140‑1 (MQ=255)
                                                                                                               ggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGa                            >  1:5331856/1‑140 (MQ=255)
                                                                                                               ggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGa                            >  1:6167560/1‑140 (MQ=255)
                                                                                                               ggCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGa                            >  1:626134/1‑140 (MQ=255)
                                                                                                                gCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTTGACGGTTAAACCGTTCTTTTTCAGGAg                           >  2:3209430/1‑140 (MQ=255)
                                                                                                                gCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAg                           >  2:616312/1‑140 (MQ=255)
                                                                                                                    tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGa                                                    >  2:659252/1‑111 (MQ=255)
                                                                                                                    tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTg                       >  2:6916691/1‑140 (MQ=255)
                                                                                                                    tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTg                       >  2:4800528/1‑140 (MQ=255)
                                                                                                                    tCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTg                       >  1:366119/1‑140 (MQ=255)
                                                                                                                         ccaccGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTAAAAACAACAGAAGCAAAGGCTTCATTTACTTCAAAAAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTAAGGAGCTTGTTAAt                  >  1:4558315/1‑140 (MQ=255)
                                                                                                                          caccGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTGTTTTTCAGGAGCTTGTTAAtt                 >  2:3502317/1‑140 (MQ=255)
                                                                                                                             cGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCa              <  1:6988684/140‑1 (MQ=255)
                                                                                                                             cGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCa              <  1:7615522/140‑1 (MQ=255)
                                                                                                                               tttAGATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATAGTGGAAGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCaaa            >  2:278161/1‑140 (MQ=255)
                                                                                                                               tttACATTTACTTTTTCAAGATCGAAACCGAGGATTTGTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAATCAAGTCAATATCTTGGACGGTTAACCCGTTCTTTTTCAGGAGCTTGTTAATTGCaaa            >  2:1528018/1‑140 (MQ=255)
                                                                                                                               tttACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCaaa            >  2:5205845/1‑140 (MQ=255)
                                                                                                                               tttACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCaaa            >  2:189245/1‑140 (MQ=255)
                                                                                                                                      ttACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTGCTTCAAACAAGTCAATATCTTGGACGGTTACACCGTTCTTTTTGAGGAGCTTGTTAATTGCAAAACCCGGa     >  1:3926097/1‑140 (MQ=255)
                                                                                                                                       tACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAg    <  1:918058/140‑1 (MQ=255)
                                                                                                                                        aCTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAACACAACAGAAGCACCCGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGc   <  1:1854197/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AGCTGCACCGCTGCAAATGGCGGCAACACCCAGTCCCCCTCCCCGTCGCTTTAATTCGTAAACAAGTGTCATGAGTATTCTCGCACCGCTTGCGCCGATCGGGTGGCCGAGGGCGATCGCACCACCGTTTACATTTACTTTTTCAAGATCGAAACCGACGATTTTTTTACATGTCAAAACAACAGAAGCAAACGCTTCATTTACTTCAAACAAGTCAATATCTTGGACGGTTAAACCGTTCTTTTTCAGGAGCTTGTTAATTGCAAACCCCGGAGCT  >  NZ_CP034943/2386977‑2387253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: