Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,502,242 T→G 20.0% T274P (ACG→CCG)  hrcA ← heat‑inducible transcriptional repressor HrcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,502,2420TG20.0% 365.2 / 31.7 233T274P (ACG→CCG) hrcAheat‑inducible transcriptional repressor HrcA
Reads supporting (aligned to +/- strand):  ref base T (110/66);  new base G (54/2);  total (165/68)
Fisher's exact test for biased strand distribution p-value = 1.48e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 253 total aligned reads displayed.

TAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGT  >  NZ_CP034943/2502106‑2502379
                                                                                                                                        |                                                                                                                                         
  atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATGTTAATCGAAATCCCCGggtgtg                                                                                                                                      >  2:4434645/1‑140 (MQ=255)
  atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAATAAGCCGTAATCAGACTGCAGTTTTCGATCTCTTCATAGTCGTTTTCTTTCCCGATTTTATTCGAAATACCCGggtgtg                                                                                                                                      >  2:6952007/1‑140 (MQ=255)
  atTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAAACCAAATCCCCGtgtgtg                                                                                                                                      >  2:4079760/1‑140 (MQ=255)
   ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTGCCGATTTTAATCGAAATACCCGGGTGTgg                                                                                                                                     >  1:920811/1‑140 (MQ=255)
   ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGTGTgggg                                                                                                                                     >  1:4378680/1‑140 (MQ=255)
   ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAATAGAAATCCCCGTGTGTgg                                                                                                                                     >  2:2486020/1‑140 (MQ=255)
   ttCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTACCCATTTTAATAGAAATACCAGGGTGTgg                                                                                                                                     >  1:4508699/1‑140 (MQ=255)
       tGCGGGTCGGACCGTTGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGAt                                                                                                                                 >  2:7075472/1‑140 (MQ=255)
       tGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAt                                                                                                                                 >  1:6066142/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGGTTCCCGATTTTATTCGAAAACCCCGTGGGTGGGGAtt                                                                                                                                >  1:2224578/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                                                                >  1:3695638/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGAtt                                                                                                                                >  2:7260479/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGAtt                                                                                                                                >  2:1930689/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTACCGATTTTAATAGAAAACCCCGTGGGTGGGGAtt                                                                                                                                >  1:1049477/1‑140 (MQ=255)
        gCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGGTCTTTCCCGCTTTTCATCGAAATCCCCGTggggggggctg                                                                                                                                >  2:4396509/1‑137 (MQ=255)
            gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGACTAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAAc                                                                                                                            >  2:7591502/1‑140 (MQ=255)
            gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGa                                                                                                                                  <  1:46952/134‑1 (MQ=255)
            gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGACATCCCCGGGTGTGGGGATTGAAc                                                                                                                            >  2:474458/1‑140 (MQ=255)
            gTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAAc                                                                                                                            >  2:3948065/1‑140 (MQ=255)
             tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                           <  1:7371529/140‑1 (MQ=255)
             tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                           <  2:3984082/140‑1 (MQ=255)
             tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGCGTGGGGATTGAACg                                                                                                                           <  1:6208617/140‑1 (MQ=255)
             tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCGTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                           <  1:3322375/140‑1 (MQ=255)
             tCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACg                                                                                                                           <  2:344721/140‑1 (MQ=255)
                      tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTaaa                                                                                                                  <  1:7355747/140‑1 (MQ=255)
                      tgatCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCCTGTGGGGGGATTGTAAGGGCTTTaaa                                                                                                                  >  2:1927536/1‑140 (MQ=255)
                          cGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAATAGAAATACCCGTGTGTGGGGATTTAACGCGCGTTAAAACAt                                                                                                              >  2:6506196/1‑140 (MQ=255)
                            cAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATcc                                                                                                            <  1:1532956/140‑1 (MQ=255)
                            cAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATcc                                                                                                            <  1:7068430/140‑1 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTGCCGATTTTAATAGAAATCACCGGGTGTGGGGATTTAACGTGCTTTAAAAAATGCTGTTAttttta                                                                                                 >  1:503924/1‑139 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATCCCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCGGTTCTTTTTc                                                                                                 >  1:1419941/1‑140 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTTAAAGAGGTTTAAAACATACTGTTCtttttg                                                                                                 >  1:5311223/1‑139 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCCATTTTAATCGAAAAACCCGGGGGTGGGGATTGAACGAGCTTTAAAAAATACTGTTCttttta                                                                                                 >  1:4731888/1‑139 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgtgt                                                                                                                                       <  1:289585/102‑1 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCgtgtgt                                                                                                                                       >  2:289925/1‑102 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCCGGTGGGGGGATTGAACGCGCTTTAAAACAACAGGTTCTTTTTc                                                                                                 >  2:6101041/1‑140 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCCATTTTAATCGAAAACCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTc                                                                                                 >  1:7495649/1‑140 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTGTGTTTACCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGAAAGAGCTTTAAAACCTCGTTTTCTTTTTc                                                                                                 >  2:3882918/1‑140 (MQ=255)
                                       aTCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATACCCGGGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTGTTTTTc                                                                                                 >  1:3210161/1‑140 (MQ=255)
                                           gCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAAtt                                                                                             <  2:1241099/140‑1 (MQ=255)
                                            cTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATGGAAATCCCCGTGTGGGGGGATTGTACGAGCGTTAAAACCTCCTGTTCTTTTTAAATTa                                                                                            >  1:5225044/1‑140 (MQ=255)
                                                  aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTGTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAAAACCCGGGTGTGGGGCTTTAAAGAGCTTTTAAACATTCTGTTATTTTTCAATTAATgagc                                                                                      >  2:2647319/1‑139 (MQ=255)
                                                  aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTTATCGAAATCCCCGGGGGTGGGGGTTTTACGAGCTTTAAAAAATCCTGGTGTTTTTGAATTAATtaga                                                                                      >  1:6872298/1‑140 (MQ=255)
                                                  aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTACCGATTTTAATCGAAATACCCGGGTGTGGGGATTGTACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATtaga                                                                                      >  1:6373007/1‑140 (MQ=255)
                                                  aTCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATtaga                                                                                      >  1:41841/1‑140 (MQ=255)
                                                      aaggAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTATTTAACGAATTTAATCGAAAAACCCCTGTTTGCGGATTGAAAGAGCTTTAAAAAATGCTGTTCATTTTAAATTAAAAAGAGAAg                                                                                  >  1:1596535/3‑140 (MQ=255)
                                                      aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTGTTTCCCGATTTTAATCGAAAAGCCCGGGGGTGGGGATTGGACGAGCTTTTAAACATCCTGTTCTTTTTATATTAATGAGTGAAg                                                                                  >  1:3241645/1‑140 (MQ=255)
                                                      aCGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAAAATACGGTTCTTTTTGAATTAATTATAGAAg                                                                                  >  1:6817247/1‑140 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTGTTTTTCAATTAATGAGAGAAGc                                                                                 >  2:5609097/1‑140 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTATTTTTAAATTAATTAGAGAAGc                                                                                 >  2:6837693/1‑140 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAAACCCGGGTGTTGGGATTGAACCAGCTTTAAAAAATGCGGTTATTTTTAAATTAATTAGAGaatc                                                                                 >  1:5512763/1‑138 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGGGTGTGGGGATTGAAAGTGCGTTAAAACATCCGGTTGTTTTTAAATTAATgagattagc                                                                                 >  1:1818251/1‑135 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTTAAAAATCCTGTTCTTTTTCAATTAATTTGAGAAGc                                                                                 >  1:2266837/1‑140 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATAGAAATACCCGTGTGTGGGGATTGTACGAGCGTTAAAACAGCCTGTTCTTTTTCAATTAATGAGAGAAGc                                                                                 >  1:3545742/1‑140 (MQ=255)
                                                       cGGAATAAGAAGCCGTAATCAGACTGCAATTTTCCATCTCTTCATAGTCGTTGTATTTTACGATTTTAATCGAAATTAACCAGTGAGGGGATTGAACCACCATTAAAAAAACCAGTTGATTGTGAAATAATgaaaagacc                                                                                 >  1:733796/1‑133 (MQ=255)
                                                               gaagCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAAc                                                                         <  2:2197293/140‑1 (MQ=255)
                                                                 agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTACCGATTTTAATAGAAAACCCCGGGGGGGGGGATTGTAAGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCgaacgaacgc                                                                       >  1:2150819/1‑138 (MQ=255)
                                                                 agCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTGCCGCTTTTAATCGAAATACCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATTATTGTAGCGACCGAActc                                                                       >  1:378935/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTgg                                                                    >  2:1713513/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAAAACCCGGGGGTGGGGATTTAACGAGGTTTAAAACATCCTGTTCTTTTTAAATTAATGAGAGAAGAGACCGAActcttg                                                                    >  2:6342386/1‑138 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGAATTTAATAGAAATACCCGTGTGTGGGGATTGACAGAGCTTTAAAACATCCTGTTCTTTTTCAATTACTGAGAGAAGCGACCGAACTCTgg                                                                    >  2:5029929/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATCGAAAACCCCCTGTGTGGGGATTGAACGAGCTTTAAAAAATCCGGTTCTTTTTGAATTAATTAGAGAAGCGACCGAACTCTgg                                                                    >  1:511924/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGATTTTAATAGAAAAACCCGTGTGTGGGGGTTTAACCAGCTTTAAAAAATCCGGTTGTTTTTCAAATAATGAGTGAATCGACCCAACTCggg                                                                    >  1:2497798/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTCCCCATTTTAATCGAAAAACCCGGGTGGGGGGCTTGAACGTGCTTTAAAAAAACCTGTTGTTTTTGAATTAATGTTAGAAGCGACCGAACACTgg                                                                    >  1:6577226/1‑140 (MQ=255)
                                                                    cGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGGTTGCCGATTTTAATCGAAATACCCCGGGGGGGGGATTGAACGAGCTTTAAAAAATCCTGTTGTTTTTGAATTAATGGGAGTATCGACCCAACGCTgg                                                                    >  1:2637375/1‑140 (MQ=255)
                                                                     gTAATCAGAGTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTGTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                                                   <  2:4255930/140‑1 (MQ=255)
                                                                     gTAATCAGACTGCAGTTTTCAATCTATTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGACCGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGt                                                                   <  2:5175342/140‑1 (MQ=255)
                                                                      tAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTGTTGTTTGCCGCTTTTTATCGAAATACCCCGGTGGGGGGATTTAACGAGCGTTAAAAAATCCGGTTGTTTTTCAATTAATGTTAGAAGCGACCGAACTCGGGTg                                                                  >  1:121198/1‑140 (MQ=255)
                                                                       aaTCAGACGGCAGTTTTCCATCTCTTCATAGTGGTTTTCTTTCCGGTTTGTAATGGAAAACCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGa                                                                 <  1:3026422/140‑1 (MQ=255)
                                                                         tCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGata                                                               <  1:1710849/140‑1 (MQ=255)
                                                                            gACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCa                                                            <  1:7260479/140‑1 (MQ=255)
                                                                             aCTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATCCCCGTGTGTGGGGATTGTACGAGCTTTAAAACATCCTGTTGTTTTTGAATTAATGAGAGAATCGACCGAACTCTGGGGATATCAt                                                           >  2:7642921/1‑140 (MQ=255)
                                                                             aCTGCAGTTTTCCATCTCTTCATAGTCGTGTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACGCTGGTGTTATCAt                                                           >  2:5430134/1‑140 (MQ=255)
                                                                                   gTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACt                                                     <  1:702219/140‑1 (MQ=255)
                                                                                      ttCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCg                                                  <  2:2224578/140‑1 (MQ=255)
                                                                                      ttCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATAGAAATACCCGGGTGTGGGGATTGTACGTGCTTTAAAACAACCGGTTCTTTTTCAAATAAAGAGAGAAGCGACCGAACGCGGGTGATATCATGGaaatacg                                                  >  1:3462975/1‑135 (MQ=255)
                                                                                       tCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATACCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTTTTCTTTTTCAATTAATGAGAGAAGCGACCGAAATCTGGTGTTATCATGGAACTCCgg                                                 >  2:5800073/1‑140 (MQ=255)
                                                                                        ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAATCATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:3695629/140‑1 (MQ=255)
                                                                                        ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:1049477/140‑1 (MQ=255)
                                                                                        ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  2:3387004/140‑1 (MQ=255)
                                                                                        ccATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGACAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGc                                                <  1:5884630/140‑1 (MQ=255)
                                                                                         cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCAAGGAACTCCGGCt                                               >  2:5899073/1‑140 (MQ=255)
                                                                                         cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGGGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGGGATATAATGGAACTCCGGCt                                               >  2:6031258/1‑140 (MQ=255)
                                                                                         cATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCCGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGGTCTTTTTCCATTAATGAGAGAAGCCACCGAACTCTGGTGATATCATGGAACTCCCGCt                                               >  2:6684520/1‑140 (MQ=255)
                                                                                           tctcTTCATAGTCGTTTTGTTTCCCCATTTTAATCGAAATACCCGTGTGGGGGGATTTAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAACCGACCGAACTCTGGTGATATCAAGGAAATCCCGCggg                                             >  2:2866415/1‑140 (MQ=255)
                                                                                           tctcTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATACTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTgg                                             >  2:4470034/1‑140 (MQ=255)
                                                                                             tctTCATTGTCGTTTTGTTTGCGGATTTTACTCCAAATGCCTGTGTTTGGGGATTTAAGGAGCTTTAAAACATCATGTTATTTTTAAATTAATGCGATAAGGGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                           <  1:573345/140‑1 (MQ=255)
                                                                                             tctTCATAGTCGTTTTCTTTCCCGATTATAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                           <  1:5485769/140‑1 (MQ=255)
                                                                                             gctaaaaaGTCTTTTTCTTCCCCGATTTTACTCGAAATCCCCGGGTGGGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGTGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGtt                                           <  1:4396509/133‑1 (MQ=255)
                                                                                              ctTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGGGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTc                                          >  1:5685834/1‑140 (MQ=255)
                                                                                                tCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGCGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAg                                        >  2:2307356/1‑140 (MQ=255)
                                                                                                 cATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGc                                       >  2:3341791/1‑140 (MQ=255)
                                                                                                 cATAGTCGTTTTCGTTCCCGATTTTAATAGAAAAACCCGTGGGGGGGGATTGAACCAGCTTTAAAAAATCCGGTTCTTTTTCAATTAATGAGAGAATCGACCGAACACGGGTGATATCATTGAACTCCGGCTGGTTCAGc                                       >  2:3179922/1‑140 (MQ=255)
                                                                                                 cATAGTCGTGTTCTTTCCCGATTTTAATCGAAAACCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGc                                       >  2:1805646/1‑140 (MQ=255)
                                                                                                         ttttGTTTGCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGTACGAGCTTTAAAACAAGCTGTTGTTTTTCAATTAATGAGAGAAGCGAACGGACTCTGGTGTTATCATGGAACTACGGCGGGTTCAGCATATTGAt                               >  2:4690264/1‑140 (MQ=255)
                                                                                                         ttttCTTTCCCGATTTTAATAGAAAAACCCCTGTGTGGGGATTGAACGAGCTTTAAAACATACAGTTATTTTTCAATTAATGAGATAAGCGACCGAAATCTGGTGATATCATGGAACACCGGCTGGTTCAGCATATTTAt                               >  2:2388129/1‑140 (MQ=255)
                                                                                                         ttttCTTTCCCCATTTTAATAGAAATACCCGTGTGTGGGGATTGAACGAGCGTTAAAAAATCCTGTTATTTTTGAAATAATGAGAGAATCGACCGAACACTGGGGATATCATGGTAATCCCGCTGGTTCAGCAAATTTAt                               >  2:4392324/1‑140 (MQ=255)
                                                                                                         tgttctttcCCGATTTTAAACGAAATACCCGGGTGTGGGGATTGAACGAGCTTTAAAAAATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCCAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTTAt                               >  2:623995/3‑140 (MQ=255)
                                                                                                                                    cccGTGTCTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAAc    <  1:7536919/140‑1 (MQ=255)
                                                                                                                                      cGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGt  >  2:3104852/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                         
TAATTCATGCGGGTCGGACCGATGATCGCAATTGAGCCGATCTGCTTCTGATCAACGGAATAAGAAGCCGTAATCAGACTGCAGTTTTCCATCTCTTCATAGTCGTTTTCTTTCCCGATTTTAATCGAAATCCCCGTGTGTGGGGATTGAACGAGCTTTAAAACATCCTGTTCTTTTTCAATTAATGAGAGAAGCGACCGAACTCTGGTGATATCATGGAACTCCGGCTGGTTCAGCATATTGATTTTTCCGCCGAAAAACAATTTTTCAACGT  >  NZ_CP034943/2502106‑2502379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: