Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 138,085 C→A 5.5% I19I (ATC→ATA rpsS → 30S ribosomal protein S19

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP034943138,0850CA 5.5% 2050.8 / 29.5 712I19I (ATC→ATArpsS30S ribosomal protein S19
Reads supporting (aligned to +/- strand):  ref base C (216/437);  new base A (52/6);  total (269/443)
Fisher's exact test for biased strand distribution p-value = 1.22e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 715 total aligned reads displayed.

CGTCGTCGTAAAAATAAATAACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATCTCTGAAGATA  >  NZ_CP034943/137951‑138224
                                                                                                                                      |                                                                                                                                           
cgtcgtcgtAAAAATAAATAACGGGGTTGTATACGGTTCACTTCGGACCGTAGTTCAATCACGAAGGGAGGTTCCAAAATGTCTCGCAGCTTGAAAAAATGAACATTTGTCGATGGACACTTGATGAAAAAAATAGAGaa                                                                                                                                        >  2:1520664/1‑140 (MQ=255)
 gtcgtcgtAAAAATAAATAACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGaaa                                                                                                                                       <  1:4046037/140‑1 (MQ=255)
     tcgtAAAAATAAATAACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTaa                                                                                                                                   >  1:6356692/1‑140 (MQ=255)
      cgtAAAAATAAATAACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTaaa                                                                                                                                  >  1:2393494/1‑140 (MQ=255)
           aaataaatAACGGTGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAATAAGGACCATTTTTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGaaa                                                                                                                             <  2:4548144/140‑1 (MQ=255)
           aaataaatAACGGGGTTGTCTACGTTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGaaa                                                                                                                             <  2:583210/140‑1 (MQ=255)
            aataaataACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAAc                                                                                                                            <  1:127810/140‑1 (MQ=255)
            aataaataACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAAc                                                                                                                            <  2:5051679/140‑1 (MQ=255)
               aaataaGGGGGTTTTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGa                                                                                                                         <  1:6812042/140‑1 (MQ=255)
                       gggTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAAc                                                                                                                 <  2:7350954/140‑1 (MQ=255)
                                    ttCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAAc                                                                                                    >  1:5725704/1‑140 (MQ=255)
                                    ttCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACATGATTACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTACTaaaaaa                                                                                                    >  2:3383202/1‑139 (MQ=255)
                                     tCACTTCGGACCGTAGTGCAATCACGAAGGGAGCTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACt                                                                                                   <  1:4998811/140‑1 (MQ=255)
                                      cACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATGTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGAAAAAACtt                                                                                                  >  1:1926413/1‑140 (MQ=255)
                                       aCTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATTGCTCGCAGCTTGAAAAAAGTACCATTTTTCGATGGACACTTTATGAAAAAAATAGAGAAAATAAATTAAAAAGAAAAGAAAAAAGTCGTAAAAAcatt                                                                                                 >  2:3221200/1‑137 (MQ=255)
                                       aCTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTTTCGATGGACACTATATGAGAAAAATAGAGAAATTAAATGAAAGAGACAATAAACAACTCGTAATAAATTg                                                                                                 >  2:622827/1‑140 (MQ=255)
                                       aCTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTaaa                                                                                                                                  >  2:2483994/1‑107 (MQ=255)
                                       aCTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTg                                                                                                 >  1:2833497/1‑140 (MQ=255)
                                       aCTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTg                                                                                                 >  1:1220853/1‑140 (MQ=255)
                                           cGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGtct                                                                                             <  2:2342771/140‑1 (MQ=255)
                                             gACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCtcg                                                                                           >  1:7032867/1‑140 (MQ=255)
                                             gACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCtcg                                                                                           <  2:1089434/140‑1 (MQ=255)
                                             gACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCtcg                                                                                           <  1:5602219/140‑1 (MQ=255)
                                             gACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCtcg                                                                                           <  2:633265/140‑1 (MQ=255)
                                              aCCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGTCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAATCTTGGTCtcgt                                                                                          >  1:2963158/1‑140 (MQ=255)
                                              aCCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCtcgt                                                                                          >  1:251710/1‑140 (MQ=255)
                                              aCCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTAGCAGCTTGAAAAAAGCACAATTTTTCGATGGAAACTAGAAGACAAAAATAGAGAAATGAAAAAAAACACACCACAAAAAAGTCGAAAAAACATGGTCtagt                                                                                          >  2:4731905/1‑140 (MQ=255)
                                                  tAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTAGTAAAAACTTGGTATCGTCGtt                                                                                      >  2:588816/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTTTCGATGGACACTTGATGACAAAAATAGAGAAATTAAATGAAAAAGACAAGAAAAAAGTCGTAAAAACATTGTCTCTTCGtta                                                                                     >  2:5314658/1‑139 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTc                                                                                     >  1:725130/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTc                                                                                     >  1:3439388/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTc                                                                                     >  1:1412888/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTc                                                                                     >  2:273462/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTc                                                                                     >  1:61216/1‑140 (MQ=255)
                                                   aGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAACAAACAAGTCGTAAAAACTTAGTCTCGTCGtta                                                                                     >  2:4047449/1‑139 (MQ=255)
                                                    gTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTct                                                                                    <  2:4653508/140‑1 (MQ=255)
                                                      gCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTctac                                                                                  <  2:6695492/140‑1 (MQ=255)
                                                       cAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTctact                                                                                 >  1:4800245/1‑140 (MQ=255)
                                                          tCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACCAGTCGTAAAAACTTGGTCTCGTCGTTCTACTAtt                                                                              <  2:258155/140‑1 (MQ=255)
                                                          tCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTAtt                                                                              >  1:817904/1‑140 (MQ=255)
                                                            aCGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTAtttt                                                                            >  1:3304335/1‑140 (MQ=255)
                                                               aaGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTccc                                                                         >  1:5510977/1‑140 (MQ=255)
                                                               aaGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTccc                                                                         >  1:4439395/1‑140 (MQ=255)
                                                               aaGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGAAACTTGATGACAAAAATCGAGAAATTAAATAAAACAGACAATAAACAAGTAGTAAAAACTTGGTCTCGTCGTTCTACTAATTTcac                                                                         >  2:7517243/1‑138 (MQ=255)
                                                                aGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCca                                                                        <  2:83295/140‑1 (MQ=255)
                                                                aGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCca                                                                        <  2:372849/140‑1 (MQ=255)
                                                                aGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCca                                                                        <  2:2811956/140‑1 (MQ=255)
                                                                aGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCca                                                                        <  1:4079925/140‑1 (MQ=255)
                                                                    aGGTTCCAAAATGGCTCGAAGCTTGAAAAAAGGACCATTTGTAGATGGACACTTGATTACAAAAATCGAGAAATTAAATGAAACAGACAAGAAAAAATTCGTAAAAACTTGGTCTCTTCGTTATACTAATTTACCACAGt                                                                    >  2:7162956/1‑140 (MQ=255)
                                                                      gTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGttt                                                                  <  1:5944415/140‑1 (MQ=255)
                                                                      gTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGCACACTTTATGACAAAAATCGAGAAATTAAATGAAACAGACAATAAACAACTCGTAAAAACTTGGTCTCGTCGTTCTACTAAATTCAAACAGttt                                                                  >  2:4322933/1‑140 (MQ=255)
                                                                        tCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAAc                                                                                                                            <  1:5877537/80‑1 (MQ=255)
                                                                         ccACAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACAATTGATGACAAAAATCGAGAAATTAAATGAAACAGACAACAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTAtt                                                               <  2:6107837/140‑1 (MQ=255)
                                                                            aaaTGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATTAAAAAAATCGAGAAATTAAATGAAAAAGAAAAGAAACAAGTAGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCACACAGGTTATTGGt                                                            >  2:220331/1‑140 (MQ=255)
                                                                            aaaTGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTgtt                                                            >  1:6660225/1‑138 (MQ=255)
                                                                            aaaTGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGt                                                            <  1:526572/140‑1 (MQ=255)
                                                                              aTGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGCCAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTca                                                          <  2:1267922/140‑1 (MQ=255)
                                                                                ggCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTcaca                                                        >  2:6844519/1‑140 (MQ=255)
                                                                                 gCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTccc                                                                         <  2:3041713/122‑1 (MQ=255)
                                                                                 gCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTCATGACAAAAAAAGAGAAATTAAATTAAACAGACAAGTAAAAAGACGTAAAAACTTAGTCTCGACGTTCTACAATTTTCCCACATTTTATTGGTcacac                                                       >  2:5794037/1‑140 (MQ=255)
                                                                                      catcTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCTAGAAATTAAATGAAACAGACAACAAACAACTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  2:6013526/137‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGCCCATGGGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  2:6529785/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  2:3913810/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  2:4180508/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  1:2144939/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  1:1510664/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  1:4692505/140‑1 (MQ=255)
                                                                                      cAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCg                                                  <  1:4367179/140‑1 (MQ=255)
                                                                                       aGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAACTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGc                                                 <  2:4844820/140‑1 (MQ=255)
                                                                                        gCTTGAAAACAGGACCATTTGTCGATGGACACTTGATGCCAAAAATCGAGAAATTAAATGAAACAGACAAGAAAAACGTCGTAAAAACTGGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCa                                                <  1:2271358/140‑1 (MQ=255)
                                                                                        gCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACTAAAATCGAGAAATTAAATGAAACAGACAAGAACCAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCa                                                <  2:5170592/140‑1 (MQ=255)
                                                                                        gCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCa                                                <  1:103390/140‑1 (MQ=255)
                                                                                                 aaGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgac                                       <  1:3198019/140‑1 (MQ=255)
                                                                                                  aGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGCCCAGACACAAGTCGTAAACACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacg                                      <  1:1839676/140‑1 (MQ=255)
                                                                                                  aGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacg                                      <  2:3569439/140‑1 (MQ=255)
                                                                                                  aGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacg                                      <  2:494609/140‑1 (MQ=255)
                                                                                                   ggACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATTAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacgg                                     <  2:5556563/140‑1 (MQ=255)
                                                                                                   ggACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacgg                                     >  1:4227672/1‑140 (MQ=255)
                                                                                                   ggACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATgacgg                                     >  1:5383628/1‑140 (MQ=255)
                                                                                                       cATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGt                                 <  1:3654653/140‑1 (MQ=255)
                                                                                                              cGATGGACACTTGATGACAAAAATCGATAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACg                          <  2:700740/140‑1 (MQ=255)
                                                                                                              cGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACg                          <  2:1360754/140‑1 (MQ=255)
                                                                                                              agATGTACACTTGATGAAAAAAATCGAGAAATTAAATGAAAAAGACAAGAAACAAGTAGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACATTTTATTGGTCACACAATCTCAGTCTATGACGGACGTAAACACg                          <  1:7433041/139‑1 (MQ=255)
                                                                                                                 tGGACACTTGATGACAAAACTCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGc                       <  1:6503470/140‑1 (MQ=255)
                                                                                                                 tGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGc                       <  2:1944774/140‑1 (MQ=255)
                                                                                                                 cgcacacTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGACACAAGTCGTAAAAACTTGGTATCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGc                       <  1:2642997/137‑1 (MQ=255)
                                                                                                                  ggACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGcc                      <  1:484791/140‑1 (MQ=255)
                                                                                                                        ttGATGACAAAAATCGAGAAATTAAATGAAACAGACCAGAAACAACTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCAGAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTAtt                <  2:1609403/140‑1 (MQ=255)
                                                                                                                        ttGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTTTTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTAtt                <  2:1737235/140‑1 (MQ=255)
                                                                                                                        ttGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTAtt                <  2:5670701/140‑1 (MQ=255)
                                                                                                                           atgaCAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCAt             <  1:1685619/140‑1 (MQ=255)
                                                                                                                             gaCAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCAtct           >  1:3775580/1‑140 (MQ=255)
                                                                                                                              aCAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCAtctc          >  1:3077745/1‑140 (MQ=255)
                                                                                                                               cAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATctct         <  1:357118/140‑1 (MQ=255)
                                                                                                                               cAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATctct         <  2:7647665/140‑1 (MQ=255)
                                                                                                                               cAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATctct         <  1:6896502/140‑1 (MQ=255)
                                                                                                                                 aaaaTCGAGAAATTAAATGAAACAGACAAGAAACACGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTATATGACGGACGTAAACACGTGCCTGTATTCATCTCTGa       <  1:4532307/140‑1 (MQ=255)
                                                                                                                                 aaaaTCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATCTCTGa       <  2:2132138/140‑1 (MQ=255)
                                                                                                                                 aaaaTCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATCTCTGa       <  1:3503691/140‑1 (MQ=255)
                                                                                                                                  aaaTCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATCTCTGaa      >  2:6735541/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                           
CGTCGTCGTAAAAATAAATAACGGGGTTGTCTACGGTTCACTTCGGACCGTAGTGCAATCACGAAGGGAGGTTCCAAAATGGCTCGCAGCTTGAAAAAAGGACCATTTGTCGATGGACACTTGATGACAAAAATCGAGAAATTAAATGAAACAGACAAGAAACAAGTCGTAAAAACTTGGTCTCGTCGTTCTACTATTTTCCCACAGTTTATTGGTCACACAATCGCAGTCTATGACGGACGTAAACACGTGCCTGTATTCATCTCTGAAGATA  >  NZ_CP034943/137951‑138224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: