BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,678,408	G→T	6.5%	F333L (TTC→TTA)	ysxE ←	spore coat protein YsxE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,678,408	0	G	T	6.5%	1018.6 / 6.7	439	F333L (TTC→TTA)	ysxE	spore coat protein YsxE
Reads supporting (aligned to +/- strand): ref base G (187/192); new base T (2/48); total (190/249)
Fisher's exact test for biased strand distribution p-value = 3.33e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 454 total aligned reads displayed.

AATCAGAACCATTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAGCACC  >  NZ_CP034943/2678269‑2678546

aaTCAGAACCATTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTgg                                                                                                                                            <  2:7467000/140‑1 (MQ=255)

      aaCCATTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAAtttt                                                                                                                                      <  2:1541362/140‑1 (MQ=255)

      aaCCATTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAAtttt                                                                                                                                      <  2:7505801/140‑1 (MQ=255)

      aaCCATTTTCTTTCCGACAACCGTTGATGTGTTGCTGCTCGCAATCCTAATCTGTTTAGCTTGTGCTCGTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCCTTAAGACGTGGACGCGTTTTCAGCCTGGAAtttt                                                                                                                                      <  1:5598996/140‑1 (MQ=255)

          aTTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTtct                                                                                                                                  <  2:2236739/140‑1 (MQ=255)

           ttttCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTtctc                                                                                                                                 <  1:1836159/140‑1 (MQ=255)

                tttcCGACAACCTTTGAGGGTTTGCTGCTCGCAATCCTCATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGtt                                                                                                                            <  1:4147354/140‑1 (MQ=255)

                  tcCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTTAGCCTGGAATTTTTTCTCTTTTTCa                                                                                                                          >  1:2954258/1‑140 (MQ=255)

                  tcCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCa                                                                                                                          >  1:77032/1‑140 (MQ=255)

                  tcCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCa                                                                                                                          >  2:5796349/1‑140 (MQ=255)

                          ccTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAAtttt                                                                                                                                      <  1:7532900/120‑1 (MQ=255)

                                     ttGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTTTTCAGTCTGATGAATTTtcatca                                                                                                       >  2:5105175/1‑140 (MQ=255)

                                         tgctCGCAATCCTAATCTTTTTAGTTTGTGCTATTCATTTAGAATGTATATAAACAAACAAGTCCGGGCCATTAAGACGTGGACTCGTTTTCAGCCTGTAATTTTTTCACTTGTTAAGTCTGTTGAATTTTAATcacaca                                                                                                   <  2:7598913/140‑3 (MQ=255)

                                         tgctCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACaaa                                                                                                   <  2:2574349/140‑1 (MQ=255)

                                         tgctCGAAATCCTAAAATTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGAATCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACaaa                                                                                                   <  2:1756295/140‑1 (MQ=255)

                                         tgctCGAAATCATAATGTTTTTAGCTTGTGCTATTCATTTATAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACaaa                                                                                                   <  2:4276727/140‑1 (MQ=255)

                                         ggctCGCAATCCTAATTTTTTTCGCTTGTGCTCTTAATTTAGAATGGATATAAAAAAACAAGTCCGCGCCATTAAGAGGTGGAATCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCAACACaaa                                                                                                   <  1:7370509/139‑1 (MQ=255)

                                            ccGCAATCCGAATCTTTTTAGCTGGGGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTGTTGTTCAGTCTGATGAATTTTCATCACAAACgg                                                                                                <  2:4365148/139‑1 (MQ=255)

                                             cGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGc                                                                                               >  1:4816355/1‑140 (MQ=255)

                                              gCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGAAATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGTAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGct                                                                                              <  2:3271044/140‑1 (MQ=255)

                                              gCAATCCTAATCTTTTTAGCTTGTGCTCTACTTTTAGAATGGATATAAAAAAACAAGTCCGCCCCATTAACACGTGCACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCCGTCTGATGAATTTTCAACACACACGGct                                                                                              <  1:3358747/140‑1 (MQ=255)

                                                aaTCCTAATCTTTTTAGCTTGTGCTCTTCATTTATAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCATGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGctct                                                                                            <  2:476328/140‑1 (MQ=255)

                                                           ttttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAt                                                                                 >  1:3118316/1‑140 (MQ=255)

                                                            tttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAtt                                                                                >  1:500299/1‑140 (MQ=255)

                                                            tttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAtt                                                                                >  1:1424845/1‑140 (MQ=255)

                                                            tttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAtt                                                                                >  1:3475156/1‑140 (MQ=255)

                                                            tttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAtt                                                                                >  2:2529504/1‑140 (MQ=255)

                                                            tttAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATAACAAACGGCTCTATATTTTTCAtt                                                                                >  1:224164/1‑140 (MQ=255)

                                                            tttAGATTGTGCTATTAATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAtt                                                                                <  1:5759510/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTCGAATGGATATAAACAAACAAGGCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGTAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  1:6883190/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGATTGGATATAAACAAACAAGTCGGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCGGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  2:5380294/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGAATGTATATAAAAAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCATTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  2:1074994/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCTTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  2:6619213/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCCGTCTTATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  1:3900815/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  2:3773968/140‑1 (MQ=255)

                                                                   tgtgCTCTTCATTTAGAATGGATATAAAAAACAAAGTCCGCGCCATTAACACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCTGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  1:2589955/140‑1 (MQ=255)

                                                                   tgtgCTCTTAATTTATAATGGATATACACAAACAAGTCCGCGCAATTAAGAGGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGTTGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  2:6799437/140‑1 (MQ=255)

                                                                   tgtgCTCTACATTTAGAATGGATAAAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGTAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAAt                                                                         <  1:2028721/140‑1 (MQ=255)

                                                                    gtgCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATa                                                                        <  1:5300723/140‑1 (MQ=255)

                                                                    gtgCTCTAAATTTAGACTGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATa                                                                        <  1:4208342/140‑1 (MQ=255)

                                                                     gagacagcATTTCGAAGTGATATAAACAACCAAGTCCGGGCAATTAAGACGTGGACTGGTTTTCAGCCTGTAATTTTTTCTCTTTTTAAGTGTTATTATTTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATaa                                                                       <  1:4609663/133‑1 (MQ=255)

                                                                        tctgaaTTTAGAATGTATATACACAAACACTTCCGCGCTATAAAGACGTGGAATCGTTTTACGCATGGAATTTTTTCTCTTGTTCAGTCGGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGcc                                                                    <  1:5451262/135‑1 (MQ=255)

                                                                        tctTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGTACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGcc                                                                    <  2:7681468/140‑1 (MQ=255)

                                                                          ttCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGGTCTATATTTTTCATTTGGCCATAAGCCtt                                                                  >  2:4453060/1‑140 (MQ=255)

                                                                               ttAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTtaata                                                             >  1:764196/1‑140 (MQ=255)

                                                                               ttAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGACAATAAGCCTTtaata                                                             >  1:1980048/1‑140 (MQ=255)

                                                                                    aTGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGc                                                        >  2:726238/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGACTTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATAGTTTTCATTTGGCCATAAGCCGTTAATAATTGCGTCGCTTCAttttt                                          >  1:6040954/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCAttttt                                          >  2:1710172/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCAttttt                                          >  1:123674/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCAttttt                                          >  1:517529/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCAttttt                                          >  1:3773454/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATGCAttttt                                          >  2:6963384/1‑140 (MQ=255)

                                                                                                  acaaGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAGTTGCCAATAAGCCTGTAATAATTGCGTACATTCAttttt                                          >  2:6029379/1‑140 (MQ=255)

                                                                                                       tCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCAt                                     >  2:3884177/1‑140 (MQ=255)

                                                                                                        ccGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTTTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATc                                    <  2:6063048/140‑1 (MQ=255)

                                                                                                        ccGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATc                                    <  2:4994501/140‑1 (MQ=255)

                                                                                                        ccGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGCAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTGAATAAGTGCGTACATTCATTTTTTTCATc                                    <  1:6153798/140‑1 (MQ=255)

                                                                                                         cgcgCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTGTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAATCGGCTCTATCTTTTTCATTTGCCAATAAGCCGTTAATCATTGCGTACATTCCTTTTTTTCATcc                                   >  2:2701840/1‑140 (MQ=255)

                                                                                                                ttAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTAAATTCATTTTTTTCATCCTGTCttt                            >  2:5741631/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAt                                                                                 >  2:7098374/1‑79 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCAt                                                                                 <  1:7098374/79‑1 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTcc                         >  1:5918448/1‑135 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  2:5631710/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  2:4098804/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  1:2779848/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  2:1195750/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  1:5392213/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  1:5650204/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  2:2961636/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  2:6698995/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGtt                    >  1:1045861/1‑140 (MQ=255)

                                                                                                                        tGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTAATCCTGTCTTTTCCCGGtt                    >  1:4707694/1‑140 (MQ=255)

                                                                                                                            cTCGTTTTCAGCCGTGAATTTTTTATCTGTTTCATTCTGATTAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGGCTTTAATAATTGCGTACATTCATTTTTGTCATCCTGTCTTTTCACGGTTTGAt                <  1:7183662/140‑1 (MQ=255)

                                                                                                                              cgatttCAGCCTTGAATTTTTTCTCTTGTTAAGTATGATGAATTTTCATAACAAACGGCTCTATATTTTTCATTTGCCACTAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:1505061/137‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCGTTGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCAAAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:5110782/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATTAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  2:1345888/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  2:4807182/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:4416160/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  2:517529/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTGAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  2:652009/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCGGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:2406977/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCGGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:6726167/140‑1 (MQ=255)

                                                                                                                              cGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTAGCATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATaa              <  1:5835/140‑1 (MQ=255)

                                                                                                                               gTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAAc             >  2:3684825/1‑140 (MQ=255)

                                                                                                                                     atccTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTAATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  2:3478189/138‑1 (MQ=255)

                                                                                                                                     aggcTGGAATTTTTTCTCTGTTTCAGTCTGTTGATTTTTCATCAAAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATACTTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  2:3189895/137‑1 (MQ=255)

                                                                                                                                     aGCCTGTAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  1:1768040/140‑1 (MQ=255)

                                                                                                                                     aGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  2:6156293/140‑1 (MQ=255)

                                                                                                                                     aGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATGTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  2:200056/140‑1 (MQ=255)

                                                                                                                                     aGCCTGGAATTTTTTCTCTTGTTCAGTATGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCa       <  2:4687471/140‑1 (MQ=255)

                                                                                                                                      gcatGGAATTTTTTCTCTTGTTCAGTCTTATTAATTTTCATCAACAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCCTCCTGTCTTTTCCCGGTTTGATAACGGTTCAg      <  2:4604/137‑1 (MQ=255)

                                                                                                                                      gCCTGTAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAg      <  2:9395/140‑1 (MQ=255)

                                                                                                                                      gCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAg      <  2:6330790/140‑1 (MQ=255)

                                                                                                                                      gCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAg      <  2:867862/140‑1 (MQ=255)

                                                                                                                                       ccTGTAATTTTTTCTATTGTTCATTCTTATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAGc     <  2:2898502/140‑1 (MQ=255)

                                                                                                                                         tGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAGCAc   >  2:7437358/1‑140 (MQ=255)

                                                                                                                                          ggAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAGCAcc  <  1:913388/140‑1 (MQ=255)

                                                                                                                                          ggAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTGCCCGGTTTGATAACGGTTCAGCAcc  <  1:6592836/140‑1 (MQ=255)

                                                                                                                                          ggAATTTTTTCTCTGGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACAGTCATTTTTTTCCTCCTGTCTTTTCCCGGTTTGATAACGGTTCAGCAcc  <  1:5789519/140‑1 (MQ=255)

AATCAGAACCATTTTCTTTCCGACAACCTTTGATGTTTTGCTGCTCGCAATCCTAATCTTTTTAGCTTGTGCTCTTCATTTAGAATGGATATAAACAAACAAGTCCGCGCCATTAAGACGTGGACTCGTTTTCAGCCTGGAATTTTTTCTCTTGTTCAGTCTGATGAATTTTCATCACAAACGGCTCTATATTTTTCATTTGCCAATAAGCCTTTAATAATTGCGTACATTCATTTTTTTCATCCTGTCTTTTCCCGGTTTGATAACGGTTCAGCACC  >  NZ_CP034943/2678269‑2678546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`