Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,812,782 T→G 8.0% K202T (AAG→ACG)  EO946_RS14520 ← SDR family oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,812,7820TG 8.0% 906.8 / 7.0 429K202T (AAG→ACG) EO946_RS14520SDR family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (194/172);  new base G (57/2);  total (254/175)
Fisher's exact test for biased strand distribution p-value = 3.02e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 430 total aligned reads displayed.

CATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGC  >  NZ_CP034943/2812645‑2812920
                                                                                                                                         |                                                                                                                                          
cATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACCAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTc                                                                                                                                          <  1:3035350/140‑1 (MQ=255)
cATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTc                                                                                                                                          >  2:1020880/1‑140 (MQ=255)
cATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTc                                                                                                                                          >  2:5521483/1‑140 (MQ=255)
cATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGATTCGCTTc                                                                                                                                          >  1:2697057/1‑140 (MQ=255)
cATTGAGACCTCCGGTGGCTTCAATGACAGGGCCTGTCACAAGGTCTGAGGTCTCCTCACACACGAAAGCGATAATCCGGGCGAGATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTc                                                                                                                                          <  1:5934709/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCATACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                     <  2:5206297/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                     <  1:6025532/140‑1 (MQ=255)
     agaCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGc                                                                                                                                     <  2:3941304/140‑1 (MQ=255)
       aCCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCtg                                                                                                                                   >  1:562562/1‑139 (MQ=255)
        ccTCCGGTGGCTTCAATGACAGTGCCTGTCACACGGTCTGAGTGCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                  <  1:3172421/140‑1 (MQ=255)
        ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                  <  1:3349687/140‑1 (MQ=255)
        ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                  <  1:2313365/140‑1 (MQ=255)
        ccTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGGTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTc                                                                                                                                  <  1:2494800/140‑1 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTGGCGTCCTGGCttgcgg                                                                                                                               >  1:7086435/1‑136 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTGTTCCTTGCCGATTAGCTTCCTGGCTTCctc                                                                                                                               >  1:7485140/1‑140 (MQ=255)
           ccGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCGTGCCGATTCCCGTCCGGGCGTCcta                                                                                                                               >  1:6852910/1‑139 (MQ=255)
                 gCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTgt                                                                                                                         >  2:3856172/1‑139 (MQ=255)
                 gCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTCGCTTCCTGGGTGCCGCGATTgt                                                                                                                         >  1:5126335/1‑139 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                       <  2:6811284/140‑1 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATg                                                                                                                       <  1:6584205/140‑1 (MQ=255)
                   ttCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGAGTGGCTTCCTGGCTTCCTCgatggatg                                                                                                                       >  1:6916119/1‑140 (MQ=255)
                    tCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGc                                                                                                                      <  1:86367/140‑1 (MQ=255)
                    tCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCGTGCTCTATTGATgg                                                                                                                      >  1:7631020/1‑139 (MQ=255)
                       aTGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGcttc                                                                                                                   <  1:3580157/140‑1 (MQ=255)
                           cAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGcttccttc                                                                                                               <  2:4588239/140‑1 (MQ=255)
                             gTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAATCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTGTCCTAGCCGATTCGCTTCCTGGATTCCTCGAGTGATGGTTCCTTCaa                                                                                                             >  2:4362532/1‑139 (MQ=255)
                              tGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGGCGATTCGCTTACTGGGTTCCACTAATGATTCGTCCGTCATg                                                                                                            >  1:587073/1‑140 (MQ=255)
                              tGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTGGCTTCCTGGCTGCCGAGATTTCTGCGTCCGTCATg                                                                                                            >  1:217185/1‑140 (MQ=255)
                              tGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTTCCGATTCGCTTGCTGGCGTCCTCGATTTATGCGTGCGTCATg                                                                                                            >  1:2417491/1‑140 (MQ=255)
                               gCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGt                                                                                                           <  1:1303012/140‑1 (MQ=255)
                               gCCTCTCAAAATGTCTCCGTTCTCCTCACACAAGAAAGCGATCATCCGGTCGATATCCTCGCCCGTGCCAGATCTTCCTATCGGCGTTTTTTCCTGGCCGATTCGCTTCCTGGCTTCCTCTCTTGAGGCTTCCTTCAtct                                                                                                           <  1:2398164/140‑3 (MQ=255)
                                ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGGTTGCTTGCTTGCTCGATTGATTCTTGCTTGATGTc                                                                                                          >  2:6457920/1‑140 (MQ=255)
                                ccTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTc                                                                                                          >  2:3554838/1‑140 (MQ=255)
                                   gTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGCTTCGCGTCCTGGCTTCCTCTATTGATGGTTCCTTCATGTCGcc                                                                                                       >  2:1946413/1‑140 (MQ=255)
                                    tCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTCGCCg                                                                                                      >  2:2398824/1‑140 (MQ=255)
                                        aaGGTCTGAGTTCTCCTCACACCAGAAAGCGATACTCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACaa                                                                                                  <  2:6742448/140‑1 (MQ=255)
                                        aaGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGCCGTCCTCTATTGATGCTGCCTTCAAGTCGCCGACaa                                                                                                  >  2:5986026/1‑140 (MQ=255)
                                        aaGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCGGTGCCAGATCGTCCTATCGGCGGTGTTTCCTTGCCGAGTCGCTTCCTGGCGGCCGGGATTGATGCGTCCTGCGTGTCGCCGACaa                                                                                                  >  2:5291552/1‑140 (MQ=255)
                                        aaGGGCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATAGCCTCGCCTGTGCCCGATCTTCCTAACGGCGTTTTTTGCTTGCCGATGCGGGTCCTGGCTGCCGCGGATGATGCTTCCGTCAGGGAGCCGACaa                                                                                                  >  2:5627758/1‑140 (MQ=255)
                                              tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGGGTCCTCTATTGATGCTTCCGTCATGTCGCCGACCAAGTCAc                                                                                            >  1:5298188/1‑140 (MQ=255)
                                              tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCAc                                                                                            <  2:6560256/140‑1 (MQ=255)
                                              tGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTTCCGATTAGCTTCCTGGCTTGCGCTATTGATGCTTCCTTGAAGTCGCCGCCAAAGTAAc                                                                                            >  1:2778961/1‑140 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTTCCAGATCTTCCTATCGGCGTTTTTTACTTGCCGATTCGCGTCCGTGCTTCCGCTGATGTTGCGTGCGTCATTGCGCCGCCCATTGGACCCggggc                                                                                     >  1:385554/1‑138 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCGTGCGGGCTTGCTGTATTGATGGTTCCTTCATGTCGCCGACCCTGTCACCCGGGCa                                                                                     >  1:2245629/1‑140 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCGCTATTGATGCTTCCTTCATGTCGCCGACAAAGTAACCCGGGCa                                                                                     >  2:2523255/1‑140 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGATTAGCTTACTGGCTTCCTCTATTGATGCTTCCATCATTTCGCCGACAAAGTCACCCGGGCa                                                                                     >  2:1166527/1‑140 (MQ=255)
                                                     tcctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTACTTGCCGATTAGCTTCCTGGCTTGCGCTATTTATTCTTCCTTCATGTAGCCGACAAAGTCACCCGGGCa                                                                                     >  1:4182133/1‑140 (MQ=255)
                                                      cctcTCACAAGAAAGCGATCATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCGTAGCGGCGTGGTTTGCTGGGCGATTCGCGTAGTGGGTTGCTCTATTGATGCGTGCGTCAGTTCGCCGGCCAAGTCACCCGGGCaa                                                                                    >  1:1673826/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTCCTGGCTGGCTCTATTGATGCTTCCTTCATGTCGCCGACCATGTCACCCGGGCaa                                                                                    >  2:3434815/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCaa                                                                                    >  2:1761694/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCAGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACCATGTCAACCGGGCaa                                                                                    >  2:3209386/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCATGCCCATTCGCTTCCTGGCGTCCTCTATTGATGCTTCCTTCATGTAGCCGAAAATTTCACCCGGGCaa                                                                                    >  2:2840401/1‑140 (MQ=255)
                                                      cctcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTCCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGAGTCGCTTGCGTGGGTGCTCTATTGATTGTTCCTTCATGTCGCCGGCAAAGTCACCCGGGcca                                                                                    >  1:108968/1‑138 (MQ=255)
                                                        tcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAc                                                                                  <  1:4255105/140‑1 (MQ=255)
                                                        tcACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAc                                                                                  <  1:3985342/140‑1 (MQ=255)
                                                         cacacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTGCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAcc                                                                                 <  2:5772400/140‑1 (MQ=255)
                                                         cacacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAAcc                                                                                 <  1:772902/140‑1 (MQ=255)
                                                          acacaAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCCAACCa                                                                                >  2:5115903/1‑140 (MQ=255)
                                                               aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTGCTTGCCGATTAGCGTCCTTGCGTCCTCGATTGTTGGTTGCTTCAAGTCGCCGCCAATGTCCACCCGGCAAACCATGTTg                                                                           >  1:974758/1‑140 (MQ=255)
                                                               aGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTGTTTTCCTTGCCGGTTCGCTTCATGGCTTCATCTCTTGAGTCTTTCGTGAAGTCGCCGACAAAGTCACCCGGGCCAACCaacttg                                                                           >  1:4900602/1‑135 (MQ=255)
                                                                 aaaGCGATAATCCGGGCGCTATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTACTTGCCCATTCGCTTGCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAAATTCACCCGGGCAAAACAAGTTGGc                                                                         >  2:2700478/1‑140 (MQ=255)
                                                                      gATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGAGTCGCTTCCTGGGGTCCTCTATTTATGCTTGCGTCATGTGGCCGACAAGTGCACCCGGGCCAACCCTGTTGGGAGTTa                                                                    >  1:1006558/1‑140 (MQ=255)
                                                                        tAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATg                                                                  <  1:678031/140‑1 (MQ=255)
                                                                          aTCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATTTCGCCGACAAAGTCACCCGGGCAAACCAAGTTGGCAGTTATGcc                                                                >  2:7575532/1‑140 (MQ=255)
                                                                              gagacagatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTAATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTAGGCCGAAt                                                            <  1:2609425/133‑1 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTGCTTGCTTCCTCTATTTATGGTTCCGTGATGTCGCCGACAATGTCACCCCGGCAAACCATGTTTGGAGTTATTCCGAAtt                                                           >  1:6345685/1‑140 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTTCCGATTCGCTTCCTGGCGTCCGCGATTTATTCTTACGTCATGTGGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAAtt                                                           >  1:1569632/1‑140 (MQ=255)
                                                                               ggCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCGTCCTCTATTTATTCGTCCTTCATGTCGCCGACAAAGTCACCCGGGCCAAACAGGTTGGGAGGTATGCCGaaat                                                           >  1:6670722/1‑138 (MQ=255)
                                                                                gCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGATTCGCTTCCTGGCTTGCTCTATTGATTCTTCCGTCATGTAGCCGACAATGTCACCCCGGCAAACCATGTTGGCAGTTATTCCGAAtta                                                          >  1:3744639/1‑139 (MQ=255)
                                                                                   atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                       <  1:4078657/140‑1 (MQ=255)
                                                                                   atatCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTGTCCTGGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                       <  2:2625127/140‑1 (MQ=255)
                                                                                   atatCCTCGCCTGTGCCAGATATTCCTATCGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTCCTCTCTTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGc                                                       <  1:1759877/140‑1 (MQ=255)
                                                                                     atCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTGCCGCTTCGCGTCCTGGCGTCCTCTATTGATTCTTCCTTAATGTCGCCGCCAAAGTCACCCCGGCAAACCCTGTTGGCCGGTATGCCCCATTAGGcgt                                                     >  1:4502603/1‑138 (MQ=255)
                                                                                          cGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTGCTTTCCCATTCGCATCCTGGCGTCCGCTATTGATGCGTCCTTCATGTCGCCGACAATGTGACCCGGGCCAACCAAGTTGGCAGTTATGCCGAAATCGGGGTCTTca                                                >  1:5429034/1‑140 (MQ=255)
                                                                                           gCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaa                                               <  1:3282055/140‑1 (MQ=255)
                                                                                           gCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaa                                               <  1:297224/140‑1 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTTGCTTTCCGCTTAGCTTCCTGGGTTCCGGTATTGATGCTTGCGTCATGTGGCCGACAAAGTAAACCCGGCAAAACATGTTTGCAGTTATGCCGTAATACGCTTCTTaaaatgca                                          >  1:7180917/1‑135 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGCTTCCCTTACTTGCCTCCTCTATTGATGCTTCCGTCATTTAGCCGACAAAGTCACCCCGGCAAACAAGAGTGGCAGTGATGACCACTTCCGCTACTAcaatagca                                          >  2:4285190/1‑140 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCTCTTTTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCCAACCATGTTGGCAGTTATGCCGACTTAGGCTTCTTcaattgca                                          >  2:6923470/1‑140 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGAGTCGCAACCTTGCGTCCGCAAATGATGCGTCCGTCAAGGCGCCGCCAAAGGCACCCGGGCCAACCAAGGTGGCACTAATGCCCAAGTCGGCTTAATcaaaggca                                          >  2:3133228/1‑140 (MQ=255)
                                                                                                tgCCAGATCTTCCTATCGGCGTTTTTGAGGTGCCGATTCGCGTCCAGGCTTGCGCTATTTATTGTTGCTGAAATTCGCCGACAATGTCCCCCGGGCAAAACAATTTTGCAGTTATGCCGAATTAGGCTTGTTccatgggc                                          >  1:4762745/1‑138 (MQ=255)
                                                                                                 gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                         <  1:5741985/140‑1 (MQ=255)
                                                                                                 gCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTcaatggcaa                                         <  1:7552132/140‑1 (MQ=255)
                                                                                                     gATCTTCCTATCGGCGTTTTTTCCTTGCCGATTAGCTTCCTGGGGTCCTCTATTGATGGTTCCTTCATGTGGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGGTTCTTCAATGGCAATGGt                                     >  2:4952567/1‑140 (MQ=255)
                                                                                                     gATCTTCCTATCGGCGTTTTTTCCTTGCCCATTCGCTTCCAGGCTTCCTCTATTGATGCGTCCTTAATGTCGCCGACAATGTCACCCGGGCAAACAATTTTGGCAGTTATGCCGAATTCGGCTTCTTCAAAGGCAATTGt                                     >  2:4133737/1‑140 (MQ=255)
                                                                                                      aTCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTc                                    <  2:1517801/140‑1 (MQ=255)
                                                                                                      aTCTTCCTATCGGCGTTTTGTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCCTGTTGGCAGTTATTCCGAATTCGGCTTCTTCAATGGCAATTGTc                                    <  1:1820174/140‑1 (MQ=255)
                                                                                                          tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCTCTATTGATGCTTCCTTCCTGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGGTATTCCGCATTCGGCGTCTTCAATGGCAATGGTCTTCg                                >  2:6141330/1‑140 (MQ=255)
                                                                                                          tCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCGCTATTGATGCTTCCTTCATGTCGCCGACAAAGTCACCCGGGCAAACCATGTTGGCAGGTATGCCCAAATCGGCGTCTTCAATGGCAATGGTCTTCg                                >  2:2237288/1‑140 (MQ=255)
                                                                                                               tCGGCGTTTTTTGCTTGCCCAATCGCGTCCTTGCTTCCTCGATTGATGCTTCCTTCATGTCGCCCACAAAGTCACCCGGGCAAACCATGTTGGCAGTTATGCCCAATTCGGCTTCTTCAATGGCAATGGTCTTCCTCaaa                           >  2:754357/1‑140 (MQ=255)
                                                                                                               tCGGCGTTTTTTCCGTGCCGATTCGCTTGCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGGTATGCCGAATTCGGCTTCTTCAATGGCAATGGTGTTCGTCaaa                           >  2:1595896/1‑140 (MQ=255)
                                                                                                                cGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGGTTCCGCTATTGATGCTTCCTTCATGTCGCCGACAATGGCACCCGGGCAAACCAGGTTGGCAGTTATGCCGAATTAGGCTTCTTCACTGGCAATGGTCTTCGTCCaag                          >  2:7121664/1‑140 (MQ=255)
                                                                                                                cGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTAGTGATGCTTCCTTCATGTCGCCGACAATGTCACCCCGGCAAACCATGTTTGCCGTTTTGCCGAATTCGGCTTCTTCAATGGCAATTGTATTAGTCAaag                          >  2:1105732/1‑140 (MQ=255)
                                                                                                                cGGCGTTTTTTCCTTGCCGATTCGCGTCCTGGCTTGCTCTATTGATGCTTCCGTCATGTCGCCGACAATGTCACCCCGGCCAACCAAGTTGGCAGTTATTCCGAATTCGGCTTCTTCAATTGCAATGGTGTTCGTCaaat                          >  2:862429/1‑139 (MQ=255)
                                                                                                                   cGTTTTTTCCTTGCCGATTCGCTTCCAGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCCAGTTGGCAGTTATTCCGAATTAGGCGTCTTCAAAGGCAATGGTCGTCGTCAaagaac                       >  2:2091289/1‑139 (MQ=255)
                                                                                                                     ttttttCCTTGCCGATTCGCTTCCTGGCTTCCTCTAGTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGcc                     >  2:7414648/1‑140 (MQ=255)
                                                                                                                        tttCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCaaa                  <  2:5429034/140‑1 (MQ=255)
                                                                                                                             ttGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCAc             >  2:1266209/1‑140 (MQ=255)
                                                                                                                             ttGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTAGTCAAAGAAGCCAAACCCAc             >  2:4077367/1‑140 (MQ=255)
                                                                                                                                    ttCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCa      <  2:298001/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CATTGAGACCTCCGGTGGCTTCAATGACAGTGCCTGTCACAAGGTCTGAGTTCTCCTCACACAAGAAAGCGATAATCCGGGCGATATCCTCGCCTGTGCCAGATCTTCCTATCGGCGTTTTTTCCTTGCCGATTCGCTTCCTGGCTTCCTCTATTGATGCTTCCTTCATGTCGCCGACAATGTCACCCGGGCAAACCATGTTGGCAGTTATGCCGAATTCGGCTTCTTCAATGGCAATGGTCTTCGTCAAAGAAGCCAAACCCACTTTCGCAGCGC  >  NZ_CP034943/2812645‑2812920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: