Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,830,550 G→T 12.9% A98S (GCA→TCA)  brnQ → branched‑chain amino acid transport system II carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,830,5500GT12.9% 794.9 / 25.4 388A98S (GCA→TCA) brnQbranched‑chain amino acid transport system II carrier protein
Reads supporting (aligned to +/- strand):  ref base G (141/183);  new base T (61/2);  total (203/185)
Fisher's exact test for biased strand distribution p-value = 2.41e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 388 total aligned reads displayed.

GTGTCGGCCTGCCGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTAAATCCTACAA  >  NZ_CP034943/2830413‑2830688
                                                                                                                                         |                                                                                                                                          
 tgtCGGCCTGCCGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTGGTTATTTAACAAAAGGACCGCTGGTTTCaa                                                                                                                                         >  1:4116905/1‑140 (MQ=255)
   tCGGCCTGCCGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTTACAATCGGACCGCTGTTTGCAAtt                                                                                                                                       >  1:4944099/1‑140 (MQ=255)
      gCCTGCCGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCcg                                                                                                                                    <  1:7285214/140‑1 (MQ=255)
           ccGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAAc                                                                                                                               <  2:4442061/140‑1 (MQ=255)
                       gttgtAGCTGTTGCTTTAAGAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGGTGGGTTTTTATGAATCGGGCCGCGGTTTTCAATTCCGCGAACAGGCACTGtttg                                                                                                                   >  1:2274054/1‑139 (MQ=255)
                        ttgtAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCAGTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGCACAGGCACGGtttgt                                                                                                                  >  1:566213/1‑138 (MQ=255)
                        ttgtAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAAACATTTTTACAGTCGTTCTTTATTTAAAAAACTGACCGCTGTTTTCAAATCCGCGAACAGGGACTGTTTca                                                                                                                  >  2:5499199/1‑139 (MQ=255)
                        ttgtAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATACGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCCCTGTTTTCAATTACGCGAACAGGCACTGTTTCt                                                                                                                  >  2:4861311/1‑140 (MQ=255)
                         tgtAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGCTTTATTTAACAATCGGACCGCTGGTTTCAATTCCGCGAACAGGCACGGTTTCtt                                                                                                                 >  1:3719103/1‑140 (MQ=255)
                           tAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCGTGCAGATAAAGCACAGCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTAt                                                                                                               <  2:7202784/140‑1 (MQ=255)
                               tGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCCCGGTTTGCAATTCCGCGAACAGGCACTGTTTGTTATTaaa                                                                                                           >  1:4398565/1‑140 (MQ=255)
                                gTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAAAAATCGGACCGCGGTTTTCAATTACCCGAACACGCCCAGTTTCTTATTaaaa                                                                                                          >  1:4159043/1‑139 (MQ=255)
                                gTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTAGTTAATTAACAAAAGGACCGCCGTTTGAAATTCAGAGAACAAGACCTGTttattagtaaaa                                                                                                          >  2:950856/1‑134 (MQ=255)
                                gTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATGTTTACAGTCGTTCTTTATTTAAAAAACGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAAt                                                                                                          >  2:1626921/1‑140 (MQ=255)
                                  tGCTTTACCAGGTACAGATGCGAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACCGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTg                                                                                                        <  1:4743018/140‑1 (MQ=255)
                                  tGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTg                                                                                                        <  1:819837/140‑1 (MQ=255)
                                  tGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTg                                                                                                        <  1:7124/140‑1 (MQ=255)
                                  tGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTg                                                                                                        <  1:5247408/140‑1 (MQ=255)
                                  tGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTg                                                                                                        <  1:5959616/140‑1 (MQ=255)
                                  tGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTATATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAAAAGGCACTGTTTCTTATTAAATTg                                                                                                        >  2:3856382/1‑140 (MQ=255)
                                   gCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGGTTTTTTTAACAATCGGACCGCGGTTTTCAATTCCGCGAACAGGCACAGTTTCTTATGTAAtttc                                                                                                       >  1:5532402/1‑138 (MQ=255)
                                       tAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCt                                                                                                   <  2:5229609/140‑1 (MQ=255)
                                           aGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTAc                                                                                               <  1:1511544/140‑1 (MQ=255)
                                             gTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAAACGGACCGCGGTTTGCAATTCCGCGAACAGGCCCGGTTTGTTATGAAATTTCGGCTGTACCt                                                                                             >  1:4983534/1‑140 (MQ=255)
                                             gTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTAACAGTCGTTCGTTATTTAAAAAAAGTACCGCGTTTTTCAATTACGCGAACAGGCAAGGTTTATTATTAAATTTCGGGTGTACCt                                                                                             >  2:2799080/1‑140 (MQ=255)
                                              tACAGATGCTCAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGGTCTTTATTTAACAATCGGACCGGTGTTTGCACTTCCGCGAACAGGCACAGTTTCTTATGAAATTGCGGCTGTACCtg                                                                                            >  2:4183168/1‑139 (MQ=255)
                                              tACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTACGCGAAAAGGCACTGTTTGTTATGAAAATGCGGCTGTACCtt                                                                                            >  2:2377424/1‑140 (MQ=255)
                                                  gATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGt                                                                                        <  1:433623/140‑1 (MQ=255)
                                                      cTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGGTTTATTTAAAAAACGGACCCCTGTTTGCAATTACCCCCAAACGCACTCTTTATTATTAAATATCGGCTGTACCAGTTGTAcgc                                                                                    >  1:6648751/1‑140 (MQ=255)
                                                       tAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAggcg                                                                                   <  2:7637229/140‑1 (MQ=255)
                                                        aaaGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAggcgg                                                                                  >  2:495562/1‑140 (MQ=255)
                                                        aaaGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAgg                                                                                     <  1:6344827/137‑1 (MQ=255)
                                                        aaaGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAAAAAACGGACCGCGGTTTTCAATTCCGCGAACAGGCACGGTTTGTTATGAAAATTCGGCTGTACCTTTTGTAtgcgg                                                                                  >  1:6970878/1‑140 (MQ=255)
                                                              cTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAAATCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGcc                                                                            >  2:2882606/1‑140 (MQ=255)
                                                               ttGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATAGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTATTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGcgg                                                                           >  2:4080623/1‑138 (MQ=255)
                                                               ttGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTAGTTAGTTAACACTCGGACCGCGGTTTTGAATTCCGCGTACAGGGCCTGGTTGTTATGACATTGCGGGGGTACCGTGTGTAgggggccagcag                                                                           >  2:1930439/1‑134 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTTCAATTACGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCgg                                                                          >  2:6675657/1‑140 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAAACATCGGACCGCGGTTTGCAATTCCGCGAACAGGCACGGTTTCTTATGAAATTTCGGCGGTACCATTTGTAGGCGGCCTGCCgg                                                                          >  1:3498650/1‑140 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGCTTTATTTAACAATCGGCCCGCTGATTGCAATACCCCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCgg                                                                          >  2:1814361/1‑140 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCgg                                                                          >  2:2160426/1‑140 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTGCGTTATTTAACAATCGGACCGCGGTTTGCAATTCCGCGAACAGGCCCGGTGTCTTATGAAATTGCGGGGGTAGCTTTTGTAGGCGGCAGGCCgg                                                                          >  1:1133100/1‑140 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGGCGTTCGTTATTTAACAAACGCACCGCGGTATGCAAATCCGCGAACACCGCCTGGTTCTTATGAAAAATCGGCAGTAGCTGTTGTTGCCCGCATGCCgc                                                                          >  2:5028842/1‑139 (MQ=255)
                                                                tGCAGATAAAGCACATCCGGTCTTTGGAACCATGTTTACAGTCGTTGTTTTTTTATACAGAGGCCCGGTGTTTGGCAATCCGCGAACAGGCACGGTTTCTTATGGAATTTGGGCGGTACCATGTGTCGGCGGCCTGCCgg                                                                          >  1:6779135/1‑140 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCACTTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGc                                                                         <  2:3442851/140‑1 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGc                                                                         <  2:1020671/140‑1 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGc                                                                         <  2:7553723/140‑1 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGc                                                                         <  2:7467680/140‑1 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGc                                                                         <  2:6602046/140‑1 (MQ=255)
                                                                 gCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAAGTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTACGCGGCATGCCGGc                                                                         <  2:6803810/140‑1 (MQ=255)
                                                                  cAGATAAAGCACAGCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCa                                                                        <  1:2047734/140‑1 (MQ=255)
                                                                    gATAAAGCACATCCGGTATTAGGAACCATATTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAAGTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATg                                                                      <  2:7399875/140‑1 (MQ=255)
                                                                       aaaGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTGTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCGGTACCTTTTGTAGGCGGCATGCCGGCATGGCt                                                                   >  2:5734729/1‑140 (MQ=255)
                                                                       aaaGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTGGTTATTTAACAATCGGACCGCTGTTTGCCATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCt                                                                   >  2:7063512/1‑140 (MQ=255)
                                                                       aaaGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCt                                                                   <  1:5904622/140‑1 (MQ=255)
                                                                       aaaGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCt                                                                   <  2:2528552/140‑1 (MQ=255)
                                                                       aaaGCACATCCGGTCTTTGGAACCATTTTGACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCt                                                                   <  2:2683878/140‑1 (MQ=255)
                                                                        aaGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCGTTATTTAACAATAGGACCGCGGTTTGCAAGTACGCGAACAGGCGCGGTTTGTTATGGAATTGCGGCTGTACCGTTTGTAGGCGGCCATCCGGCATGGCta                                                                  >  1:3268120/1‑139 (MQ=255)
                                                                            acaTCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTTACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAAATGCGGCTGTACCTTTTGTAGGCGGCATTCCGGCATGGCTCacac                                                              >  2:1840024/1‑140 (MQ=255)
                                                                               tCCGGTCTTTGGAACCATTTTTACAGTCGTTGTTTATTTAACAATCGGACCGCGGTTTTCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCGGGACCTTTTGTAGGCGGCCTGCCGGCATGGCTCACActtg                                                           >  2:2278675/1‑139 (MQ=255)
                                                                               tCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACActtc                                                           <  2:3265702/140‑1 (MQ=255)
                                                                               tCCGGTCTTTGGAACCATTTTTACAGTCGTGGTTTATTTAAAAATCGGACCGCGGTTTTGAATTACGCGAACACGCACGGGTTGTTATTAAATTGCGGGTGTAACGTTTTTAGGCGGCATGCCGGCATGGCTCACAcgtc                                                           >  1:3976438/1‑140 (MQ=255)
                                                                               tCCGGTCTGTGGAACCATTTTTACAGTCGTTCTTTATTTAAAAATCGGACCCCTGTTTGCAATTCCGCGAACAGGGACTGTTTGTTATGTAATTGCGGCTGTTCCGTGTGTATGCGGCATGCCGGCATGGCTCACActtg                                                           >  2:5627901/1‑139 (MQ=255)
                                                                                   gTCTTTGGAACCATTTTTACAGTCGTTGTTTATTTAAAAAAAGGACCGCTGTTTGCAATTCCGCGAACAGGCACAGTTTATTATGAAATTGCGGCTGTACCATTTTTAGGCGGCATGCCGGCCTGGCTCACACTTCTTAt                                                       >  2:2610418/1‑140 (MQ=255)
                                                                                    tCTTTGGAACCATTTTTACAGTCGTTGTTTATTTAACAAACGGACCGCGGTTTGCAATTCCGCGAAAAGGCACTGTTTCTTTTGAAATTGCGGCTGTACCTTTTGTAGGCGGCAATCCGGCATTGCTCACACATCTTAtt                                                      >  2:113395/1‑140 (MQ=255)
                                                                                      tttGGAACCATTTTTACATTCGTTGATAATTTAACAATAGGACCGCGGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAAATGGCTCAGTACCGTTTGTATGCGGCATGACGGCATGGCTAACAATTCTTAtttt                                                    >  2:1761102/1‑140 (MQ=255)
                                                                                        tGGAACCATTTTTACAGTCGTTGTTTATTTAAAAAAAGGACCGCGGTTTTGAAATCCGCGAACACGCACTGTTTCTTATGAAAATGCGGCGGTACCTTTTGTTGGCGGCATGCCGGCAGGGCTCACACGTGTTATTTTTa                                                  >  1:2228239/1‑140 (MQ=255)
                                                                                        tGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTa                                                  <  2:6959576/140‑1 (MQ=255)
                                                                                        tGGAACCATTTTTACAGTCGTTCTTTATTTAAAAATCGGACCGCTGTTTTCAAATCCGCGAACAGGCCCTGTTTCTTAAGAAATTTCGGCTGTACCTTTTGTAGGCGGCATTCCGGCATGGCTCACACGTCTTATTTTTa                                                  >  2:5899297/1‑140 (MQ=255)
                                                                                        tGGAACCATTTTTACAGTCGTGGGTTATTTAACAATAGGACCGCTGTTTGCAATTACGCGAACAGGCCCTGGTTCTTATGAAATTGCGGCTGTACCTTTTGTATGCGGCCAGCCGGCAAGGGTCACACATCTTATTTTTa                                                  >  1:1708779/1‑140 (MQ=255)
                                                                                          gAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACg                                                <  1:5341905/140‑1 (MQ=255)
                                                                                                tttttACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCGGTACCTGTTGTAGGCGGCATGCCGGCATGGCTCACACGTCGTATTTTTAAGtttgtt                                          >  2:5430439/1‑140 (MQ=255)
                                                                                                tttttACAGTCGTTCTTTATTTAACAATCGGACCCCTGTTTTCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGtttctt                                          >  2:6405044/1‑140 (MQ=255)
                                                                                                tttttACAGTCGTTCTTTATTTAACAAACGGACCGCGGTTTGCAATTCCGCGAACAGGCACTGTTTCGTATGAAAATGCGGCTGTACCTTTTTTAGGCGGCATGCCCGCAAGGCTCACACGTCTTAATTTTACGtttgat                                          >  2:3565520/1‑137 (MQ=255)
                                                                                                  tttACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGtttctttt                                        <  2:6296147/140‑1 (MQ=255)
                                                                                                     aCAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTttcttttctt                                     <  1:1824776/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTg                                   <  1:3620583/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTg                                   <  1:2634392/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTg                                   <  1:5134407/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTg                                   <  1:1887026/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTGTCTTTTCTTTg                                   <  2:2047105/140‑1 (MQ=255)
                                                                                                       aGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATGCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTg                                   <  1:7447896/140‑1 (MQ=255)
                                                                                                        gTCGTTCTTTAATTAAAAATCCGACCCCTGTTTTCAATTCCCCCAACAGGCACAGTTTCTTATTAAATTGCGGCTGTACCTATTGTAGGCGGCATTCCCGCATGGCTCACACTTCTTATTTTTACCtttattttaattta                                  >  2:1326994/1‑134 (MQ=255)
                                                                                                          cGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTg                                <  2:7250614/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  1:3050168/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  2:2971423/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  2:1444683/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAAGTGCGGCTGTACCTGTTGTAGGCGGCATGCCGGCATGGCTCACACTGCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  2:4780246/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTTTGCAAGTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  2:4333740/140‑1 (MQ=255)
                                                                                                            ttCTTTATTTAACAATCGGACCGCTGTGTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATGTTTACGTTTCTTTTCTTTGCTGtt                              <  2:6127522/140‑1 (MQ=255)
                                                                                                                   tttaACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTTCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGtaat                       >  1:1009059/1‑138 (MQ=255)
                                                                                                                      aaCAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTAcc                    <  1:2392669/140‑1 (MQ=255)
                                                                                                                      aaCAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTAcc                    <  1:2824230/140‑1 (MQ=255)
                                                                                                                      aaCAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTAcc                    <  1:4599460/140‑1 (MQ=255)
                                                                                                                           tCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGtt                              <  1:6159418/125‑1 (MQ=255)
                                                                                                                           tCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCt               >  2:4625290/1‑140 (MQ=255)
                                                                                                                               aCCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTaa           <  2:3027620/140‑1 (MQ=255)
                                                                                                                                   cTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTTATTcc       >  1:3905597/1‑140 (MQ=255)
                                                                                                                                    tGTTTGCAAGTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTAAATCCt      <  2:5819537/140‑1 (MQ=255)
                                                                                                                                    cgtgtGCAATTCCGCGACCAGTCACTGTGTCTTATGAAATTGCGGCTGTACCTTTTGGAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTGGCTGTTACGTACTACCTGGCTTTAAATCCt      <  2:3965876/136‑1 (MQ=255)
                                                                                                                                        tGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTAAATCCTACaa  >  1:131980/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
GTGTCGGCCTGCCGCTTTTAGGTGTTGTAGCTGTTGCTTTAACAGGTACAGATGCTAAAGGCCTTGCAGATAAAGCACATCCGGTCTTTGGAACCATTTTTACAGTCGTTCTTTATTTAACAATCGGACCGCTGTTTGCAATTCCGCGAACAGGCACTGTTTCTTATGAAATTGCGGCTGTACCTTTTGTAGGCGGCATGCCGGCATGGCTCACACTTCTTATTTTTACGTTTCTTTTCTTTGCTGTTACGTACTACCTGGCTTTAAATCCTACAA  >  NZ_CP034943/2830413‑2830688

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: