Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NZ_CP034943 2,901,671 A→C 13.5% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,901,6710AC13.5% 863.1 / 43.4 347K411T (AAA→ACA) EO946_RS14945extracellular solute‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (254/33);  new base C (3/56);  total (257/90)
Fisher's exact test for biased strand distribution p-value = 3.11e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 363 total aligned reads displayed.

GATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901532‑2901809
                                                                                                                                           |                                                                                                                                          
 aTGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATGTCGAAGAAGCTGGGACGAGAATACaaa                                                                                                                                           >  2:6735569/1‑140 (MQ=255)
            gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCTGGa                                                                                                                                >  1:4667813/1‑140 (MQ=255)
            gAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGa                                                                                                                                >  1:6266283/1‑140 (MQ=255)
             aGCTTTACTCCGACCTTTTATATTTGCAAAAGGTATTGTGGTTTGTCGTGGGCAGGGTCTCACAGGCGATTTTGGCTGAAATTTTGACCCATAAACATTTTGCTAAGAAGCTGGTATGACAATACAAACGACTCCGGGAg                                                                                                                               <  2:3593651/140‑1 (MQ=255)
             aGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAg                                                                                                                               >  2:7067098/1‑140 (MQ=255)
                 ttAGTTCGACGTTTTTTTTTTCCCAACGGGATTGTGGTTTGGCGTGGGCACGGTCGCCCCGGTGGTTTTGGCTGACTTTTTCCCGCATCAAGTCTTTTCCAAGAAGCTGGCAAGAGAATACAAACGACTCCGGGAGCAAt                                                                                                                           <  2:4842715/140‑1 (MQ=255)
                   aCTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACAAACGACTCCGGGAGCAATCg                                                                                                                         >  1:7263332/1‑140 (MQ=255)
                         aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  2:425276/1‑140 (MQ=255)
                         aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:7343666/1‑140 (MQ=255)
                         aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:6124695/1‑140 (MQ=255)
                         aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:5200715/1‑140 (MQ=255)
                         aCCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAaca                                                                                                                   >  1:4911168/1‑140 (MQ=255)
                             tttATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAgg                                                                                                               >  2:1866496/1‑140 (MQ=255)
                              ttATCCTTCCAAAAGGGATTGTGGTTTGTCCGGGGCAGGGTCGGCCTGGTGTTTATGGCTTACTTTTTGACCCCACAAGAGATTTCCAATACGCTGGTAAGACAATACACACGACTCCGGGAGCAATCGGAAACACAggg                                                                                                              <  2:5764472/140‑1 (MQ=255)
                                ataCTTGCCAAACTTATTGTGTGTTGTCCTGGGCACGGTCGCACAGGAGGTTATGGCTTAAATTTTGACGCCACAATATATTTCTAAGAAGCTGGTAAGATAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAg                                                                                                            <  2:6943666/140‑1 (MQ=255)
                                     tGCCAAACGGAATGTGGGATTTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaa                                                                                                       >  1:7512170/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGactgtctc                                                                                                      >  1:225091/1‑133 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:5147787/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:7189683/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:3337445/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:451745/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:498541/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:2402939/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  1:4138244/1‑140 (MQ=255)
                                      gCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaag                                                                                                      >  2:159805/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGACTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  2:4297326/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  1:5358348/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  1:862049/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  1:5740099/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  1:1003017/1‑140 (MQ=255)
                                       ccAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTgaaga                                                                                                     >  2:796440/1‑140 (MQ=255)
                                                       tggTCCTGGCCACGGACGCACAGGATTTTTTGGCTGAAATTTTGACGCCTCAAGAGTTTGCGAATCCGCGGGGAACAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCg                                                                                     <  2:2148219/138‑1 (MQ=255)
                                                        tGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGc                                                                                    >  2:7222067/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGACGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATCAGTGAGATCGCa                                                                                   >  1:7466783/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACTACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATTAGTGAGATCTCa                                                                                   >  2:1312186/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:1250899/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  2:6583194/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  1:6471256/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  1:2820448/1‑140 (MQ=255)
                                                         gTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCa                                                                                   >  1:4040592/1‑140 (MQ=255)
                                                                    tgTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCCAGAGATTTCGAATAAGCTGGGAAGAGAATACAAACCAGTCCGGGCGCAATCGGACACACAGGGAGCTGAAGCACAATGAGTGAGATAGCAAGAGACGTTct                                                                        >  2:5990682/2‑139 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGCGCTGAAGAACCATGAGTGAGATCGCAAGAGACGGTCa                                                                        >  2:2472747/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  1:6811715/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:345929/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:5290452/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:2236923/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:6095941/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:2082060/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:1363756/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:1925323/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGCTGACATTTTGACACCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:1086376/1‑140 (MQ=255)
                                                                    ggTCGGACAGGAGCTTATGGATGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCa                                                                        >  2:933278/1‑140 (MQ=255)
                                                                     gTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCAt                                                                       >  1:2527781/1‑140 (MQ=255)
                                                                         gACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGa                                                                   >  1:481094/1‑140 (MQ=255)
                                                                         gACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGa                                                                   >  1:5654371/1‑140 (MQ=255)
                                                                          aCAGTAGGTTATGGCTTAAATTTGGACGCCTCACGAGATTGCTAAGACGCTGGGAAGATAATACAAACGCCTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGaa                                                                  <  2:6364955/140‑1 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAActg                                                              >  1:3313506/1‑138 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                              >  1:3736627/1‑140 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                              >  1:1606869/1‑140 (MQ=255)
                                                                              gAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAg                                                              >  1:367111/1‑140 (MQ=255)
                                                                                gCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAACCAGGt                                                            >  1:5834318/1‑140 (MQ=255)
                                                                                    aTGGCTGAGATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAAGACAATCGACTCCGGTAGCAATCGGATAGACAGGGAGCTGAAGAACCATGAGTGAGATCGCACGCGAGGTTCATGTGAATCAGGTGAAg                                                        >  2:6430341/1‑140 (MQ=255)
                                                                                    aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  1:20065/1‑140 (MQ=255)
                                                                                    aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  1:3095436/1‑140 (MQ=255)
                                                                                    aTGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAg                                                        >  1:142990/1‑140 (MQ=255)
                                                                                        cTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGa                                                    >  1:924087/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACTGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  1:1932746/1‑140 (MQ=255)
                                                                                           aCATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGaaaa                                                 >  2:3252589/1‑140 (MQ=255)
                                                                                               tttGCCGCCTCACGAGATTCCGAAGACGCGGCCAACACAACACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCa                                             <  2:1441724/140‑1 (MQ=255)
                                                                                                      ccTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACTACTCCGGGAGCAATCGGAAACACAGGGAGCTGACGAACAATGAGTGAGATCGCTCGAGAAGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCGCa                                      >  1:7671021/1‑140 (MQ=255)
                                                                                                       cTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCAc                                     >  2:2633282/1‑140 (MQ=255)
                                                                                                          aaGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACttt                                  >  2:732846/1‑140 (MQ=255)
                                                                                                              tttttCGAAGAAGGTGGGACTAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtg                              <  2:5654371/138‑1 (MQ=255)
                                                                                                               ttttCGACGACGGTGGGAAGACAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:6876779/139‑1 (MQ=255)
                                                                                                               tttaggaagaaGGTGGTAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  2:6005627/135‑1 (MQ=255)
                                                                                                               tttaccaagatgCTGGTAATACAACAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  1:3922267/134‑1 (MQ=255)
                                                                                                               aTTTCTAAGACGCTGGTAAGAGAATACAAACGACTCCGCGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             <  2:3960434/140‑1 (MQ=255)
                                                                                                                 ttCGAAGAAGCTGGTAAGACAATCCAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGAt                           <  2:6177702/140‑1 (MQ=255)
                                                                                                                    taataagCTGGCAAGACAAAACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGta                        <  2:1003017/136‑1 (MQ=255)
                                                                                                                    gaagacGCTGGGAAGAGAATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGta                        <  2:405747/140‑1 (MQ=255)
                                                                                                                      agaagCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGtata                      >  2:5552120/1‑140 (MQ=255)
                                                                                                                          gCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTcc                  >  2:7608941/1‑140 (MQ=255)
                                                                                                                                acgacaaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCttt            <  2:23142/135‑1 (MQ=255)
                                                                                                                                   ataaTAAACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         <  2:3889161/138‑1 (MQ=255)
                                                                                                                                   agaATACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         <  2:4911168/140‑1 (MQ=255)
                                                                                                                                   agaATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         <  2:174271/140‑1 (MQ=255)
                                                                                                                                   agaATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         <  2:5147787/140‑1 (MQ=255)
                                                                                                                                   agaATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGct         >  2:2777080/1‑140 (MQ=255)
                                                                                                                                     aaaacacacGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:4523643/137‑1 (MQ=255)
                                                                                                                                     aaTCCACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:6612022/140‑1 (MQ=255)
                                                                                                                                     aaTACACACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  1:1289788/140‑1 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCACTCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       >  1:55423/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACATGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTCTCTTCCTGCTTTGctgt       >  2:6655779/1‑138 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTtggtgg                             >  2:7463904/1‑118 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       >  2:6990814/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       >  2:3068798/1‑140 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  2:6503252/140‑1 (MQ=255)
                                                                                                                                     aaTACAAACGACTCCGGGAGCAATAGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGATACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGctct       <  2:5454724/140‑1 (MQ=255)
                                                                                                                                        aCAAACGACTCCGGGAGCAATCGGAAACACCGGGAGCTGAAGAACAATGTGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCg    >  1:1840612/1‑140 (MQ=255)
                                                                                                                                          aaaCGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAAAAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGtt  >  1:4837228/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GATGTCAAAGTCGAGCCTTACTTCGACCGTTTATACTTGCCAAACGGAATGTGGGATGTCCTGGGCACGGTCGGACAGGAGCTTATGGCTGACATTTTGACGCCTCAAGAGATTTCGAAGAAGCTGGGAAGAGAATACAAACGACTCCGGGAGCAATCGGAAACACAGGGAGCTGAAGAACAATGAGTGAGATCGCAAGAGACGTTCATGTGAAACAGGTGAAGCCGAAAAAGCAGTCCTCACTTTGGTGGATGTATATTCCTGCTTTGCTCTCCGTT  >  NZ_CP034943/2901532‑2901809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: